Research Topics
Genomes and Genes | Greg A JohnsonSummaryAffiliation: Texas A and M University Country: USA Publications
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Publications
Pig conceptuses increase uterine interferon-regulatory factor 1 (IRF1), but restrict expression to stroma through estrogen-induced IRF2 in luminal epitheliumMargaret M Joyce
Center for Animal Biotechnology and Genomics, Texas A and M University, College Station, Texas 77843 4458, USA
Biol Reprod 77:292-302. 2007....
Secreted phosphoprotein 1 binds integrins to initiate multiple cell signaling pathways, including FRAP1/mTOR, to support attachment and force-generated migration of trophectoderm cellsJinYoung Kim
Center for Animal Biotechnology and Genomics, Texas A and M University, College Station, TX 77843, USA
Matrix Biol 29:369-82. 2010..These cell signaling pathways, acting in concert, mediate adhesion, migration and cytoskeletal remodeling of Tr cells essential for expansion and elongation of conceptuses and attachment to uterine LE for implantation...
Effects of long-term progesterone exposure on porcine uterine gene expression: progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epitheliumDaniel W Bailey
Department of Veterinary Integrative Biosciences, Texas A and M University, College Station, Texas 77843 4458, USA
Reproduction 140:595-604. 2010..We hypothesize that a servomechanism involving sequential effects of multiple hormones and cytokines, similar to those for sheep and humans, is required for GE differentiation and function, including the synthesis and secretion of SPP1...
Uterine receptivity to implantation of blastocysts in mammalsFuller W Bazer
Department of Animal Science, Texas A and M, University CollegeStation, TX 77843 2471, USA
Front Biosci (Schol Ed) 3:745-67. 2011....
Uterine histotroph and conceptus development: select nutrients and secreted phosphoprotein 1 affect mechanistic target of rapamycin cell signaling in ewesFuller W Bazer
Center for Animal Biotechnology and Genomics, Texas A and M University, College Station, Texas, USA
Biol Reprod 85:1094-107. 2011..This minireview focuses on components of histotroph that affect conceptus development in the ewe...
Osteopontin is synthesized by uterine glands and a 45-kDa cleavage fragment is localized at the uterine-placental interface throughout ovine pregnancyGreg A Johnson
Center for Animal Biotechnology and Genomics, Texas A and M University, College Station, Texas 77843, USA
Biol Reprod 69:92-8. 2003....
Conceptus-uterus interactions in pigs: endometrial gene expression in response to estrogens and interferons from conceptusesG A Johnson
Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843 4458, USA
Soc Reprod Fertil Suppl 66:321-32. 2009....
Osteopontin: roles in implantation and placentationGreg A Johnson
Center for Animal Biotechnology and Genomics, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843, USA
Biol Reprod 69:1458-71. 2003..OPN has the potential to profoundly impact pregnancy, and investigators are now challenged to focus on the mechanistic nature of the functions of this multifaceted and major component of the uterine-placental microenvironment...
Osteopontin expression in uterine stroma indicates a decidualization-like differentiation during ovine pregnancyGreg A Johnson
Center for Animal Biotechnology and Genomics, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843, USA
Biol Reprod 68:1951-8. 2003..Results suggest that uterine stromal decidualization is common to species with different types of placentation, but the extent is variable and correlates with the depth of trophoblast invasion during implantation...
Select nutrients in the ovine uterine lumen. IX. Differential effects of arginine, leucine, glutamine, and glucose on interferon tau, ornithine decarboxylase, and nitric oxide synthase in the ovine conceptusJinYoung Kim
Center for Animal Biotechnology and Genomics, Texas A and M University, College Station, USA
Biol Reprod 84:1139-47. 2011....
Stanniocalcin 1 is a luminal epithelial marker for implantation in pigs regulated by progesterone and estradiolGwonhwa Song
Center for Animal Biotechnology and Genomics, Texas A and M University, College Station, TX 77843 2471, USA
Endocrinology 150:936-45. 2009..The temporal and cell type-specific expression of STC1 makes this gene a unique marker for implantation in pigs...
Select nutrients in the ovine uterine lumen. VI. Expression of FK506-binding protein 12-rapamycin complex-associated protein 1 (FRAP1) and regulators and effectors of mTORC1 and mTORC2 complexes in ovine uteri and conceptusesHaijun Gao
Center for Animal Biotechnology and Genomics, Texas A and M University, College Station, Texas 77843 2471, USA
Biol Reprod 81:87-100. 2009..These results suggest that the FRAP1 cell signaling pathway mediates interactions between the maternal uterus and peri-implantation conceptuses and that P4 and IFNT affect this pathway by regulating expression of RHEB and EIF4EBP1...
Secreted phosphoprotein 1 (SPP1, osteopontin) binds to integrin alpha v beta 6 on porcine trophectoderm cells and integrin alpha v beta 3 on uterine luminal epithelial cells, and promotes trophectoderm cell adhesion and migrationDavid W Erikson
Department of Veterinary Integrative Biosciences, Texas A and M University, College Station, Texas 77843, USA
Biol Reprod 81:814-25. 2009....
Uterine MHC class I molecules and beta 2-microglobulin are regulated by progesterone and conceptus interferons during pig pregnancyMargaret M Joyce
Center for Animal Biotechnology and Genomics, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843 4458, USA
J Immunol 181:2494-505. 2008....
Select nutrients in the ovine uterine lumen. ii. glucose transporters in the uterus and peri-implantation conceptusesHaijun Gao
Center for Animal Biotechnology and Genomics, Departments of Animal Science and Veterinary Integrative Biosciences, Texas A and M University, College Station, Texas 77843, USA
Biol Reprod 80:94-104. 2009....
Secreted phosphoprotein 1 (osteopontin) is expressed by stromal macrophages in cyclic and pregnant endometrium of mice, but is induced by estrogen in luminal epithelium during conceptus attachment for implantationFrankie J White
Center for Animal Biotechnology and Genomics, Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843, USA
Reproduction 132:919-29. 2006..We suggest that Spp1 at the apical surface of LE provides a mechanism to bridge conceptus to LE during implantation, and that Spp1-positive macrophages within the stroma may be involved in uterine remodeling for conceptus invasion...
Integrins and extracellular matrix proteins at the maternal-fetal interface in domestic animalsRobert C Burghardt
Department of Veterinary Anatomy and Public Health, Texas A and M University, College Station, Texas 77843, USA
Cells Tissues Organs 172:202-17. 2002....
Cathepsin B, cathepsin L, and cystatin C in the porcine uterus and placenta: potential roles in endometrial/placental remodeling and in fluid-phase transport of proteins secreted by uterine epithelia across placental areolaeGwonhwa Song
Center for Animal Biotechnology and Genomics, Department of Veterinary Integrative Biosciences, and Department of Animal Science, Texas A and M University, College Station, Texas 77843 2471, USA
Biol Reprod 82:854-64. 2010..Cysteine proteases and their inhibitors may also specifically modify proteins for successful utilization and fluid-phase transport across uterine, placental, and neonatal gut epithelia...
Mechanistic mammalian target of rapamycin (MTOR) cell signaling: effects of select nutrients and secreted phosphoprotein 1 on development of mammalian conceptusesFuller W Bazer
Center for Animal Biotechnology and Genomics and Department of Animal Science, Texas A and M University, College Station, TX, USA
Mol Cell Endocrinol 354:22-33. 2012..Thus, arginine and SPP1 mediate growth, migration, cytoskeletal remodeling and adhesion of trophectoderm essential for pregnancy recognition signaling and implantation...
Select nutrients in the ovine uterine lumen. VII. Effects of arginine, leucine, glutamine, and glucose on trophectoderm cell signaling, proliferation, and migrationJin Young Kim
Center for Animal Biotechnology and Genomics, Department of Animal Science, Texas A and M University, College Station, Texas 77843 2471, USA
Biol Reprod 84:62-9. 2011....
Pig conceptuses secrete estrogen and interferons to differentially regulate uterine STAT1 in a temporal and cell type-specific mannerMargaret M Joyce
Center for Animal Biotechnology and Genomics, Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, Texas 77843, USA
Endocrinology 148:4420-31. 2007..The interactions of estrogen and interferons to regulate cell-type-specific expression of STAT1 highlight the complex interplay between endometrium and conceptus for pregnancy recognition and implantation...
Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancyDaniel W Bailey
Department of Veterinary Integrative Biosciences, Texas A and M University, College Station, Texas 77843 4458, USA
Reproduction 140:583-94. 2010..Furthermore, LE and vascular development are highly responsive to the effects of progesterone...
Interferons and progesterone for establishment and maintenance of pregnancy: interactions among novel cell signaling pathwaysFuller W Bazer
Texas A and M University, College Station, TX 77843 2471, USA
Reprod Biol 8:179-211. 2008..The magnitude of the LH surge was reduced in cows receiving endotoxin...
Select nutrients in the ovine uterine lumen. V. Nitric oxide synthase, GTP cyclohydrolase, and ornithine decarboxylase in ovine uteri and peri-implantation conceptusesHaijun Gao
Center for Animal Biotechnology and Genomics, Texas A and M University, College Station, Texas 77843 2471, USA
Biol Reprod 81:67-76. 2009..Thus, biosynthesis of NO and polyamines in ovine uterine endometria and conceptuses is potentially regulated at transcriptional, translational, and posttranslational levels to favor conceptus development and implantation...
The influence of estrogen on hepatobiliary osteopontin (SPP1) expression in a female rodent model of alcoholic steatohepatitisAtrayee Banerjee
Department of Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843 4467, USA
Toxicol Pathol 37:492-501. 2009....
Insulin-like growth factor binding protein-1 in the ruminant uterus: potential endometrial marker and regulator of conceptus elongationRebecca M Simmons
Department of Animal Science, Texas A and M University, College Station, Texas 77843, USA
Endocrinology 150:4295-305. 2009..These studies reveal that IGFBP1 is a common endometrial marker of conceptus elongation in sheep and cattle and most likely regulates conceptus elongation by stimulating migration and attachment of the trophectoderm...
Comparative aspects of implantationFuller W Bazer
Department of Animal Science Veterinary Integrative Biosciences, Texas A and M University, 2471 TAMU, College Station, TX 77843 2471, USA
Reproduction 138:195-209. 2009....
Pregnancy and interferon tau regulate major histocompatibility complex class I and beta2-microglobulin expression in the ovine uterusYoungsok Choi
Center for Animal Biotechnology and Genomics, Department of Animal Science, Texas A and M University, College Station 77843, USA
Biol Reprod 68:1703-10. 2003..The silencing of MHC class I alpha chain and beta2MG genes in the endometrial LE and sGE during pregnancy recognition and establishment may be a critical mechanism preventing immune rejection of the conceptus allograft...
Enhanced focal adhesion assembly reflects increased mechanosensation and mechanotransduction at maternal-conceptus interface and uterine wall during ovine pregnancyRobert C Burghardt
Department of Veterinary Integrative Biosciences, Texas A and M University, College Station, Texas 77843 4458, USA
Reproduction 137:567-82. 2009....
Intrauterine infusion of latency-associated peptide (LAP) during early porcine pregnancy affects conceptus elongation and placental sizeDana A Massuto
Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX, USA
Biol Reprod 82:534-42. 2010..Results suggest TGFB and integrins are contributing factors in the regulation of conceptus elongation and placental and fetal size...
Progesterone and placentation increase secreted phosphoprotein one (SPP1 or osteopontin) in uterine glands and stroma for histotrophic and hematotrophic support of ovine pregnancyKathrin A Dunlap
Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, Texas 77843, USA
Biol Reprod 79:983-90. 2008..Collectively, P(4) induces and the conceptus further stimulates SPP1 in uterine GE and stroma, where SPP1 likely influences histotrophic and hematotrophic support of conceptus development...
Interferon stimulated gene 15 conjugates to endometrial cytosolic proteins and is expressed at the uterine-placental interface throughout pregnancy in sheepMargaret M Joyce
Center for Animal Biotechnology and Genomics, Department of Veterinary Integrative Biosciences, Texas A and M University, College Station, Texas 77843-4458, USA
Endocrinology 146:675-84. 2005....
Galectin 15 (LGALS15): a gene uniquely expressed in the uteri of sheep and goats that functions in trophoblast attachmentShaye K Lewis
Center for Animal Biotechnology and Genomics, Department of Animal Science, Texas A and M University, College Station, Texas 77843, USA
Biol Reprod 77:1027-36. 2007..These results support the hypothesis that LGALS15 is uniquely expressed in Caprinae endometria and functions as an attachment factor important for peri-implantation blastocyst elongation...
Pregnancy recognition and conceptus implantation in domestic ruminants: roles of progesterone, interferons and endogenous retrovirusesThomas E Spencer
Center for Animal Biotechnology andGenomics, Department of Animal Science, Texas A and M University, College Station, TX 77843, USA
Reprod Fertil Dev 19:65-78. 2007....
Steroid regulation of cell specific secreted phosphoprotein 1 (osteopontin) expression in the pregnant porcine uterusFrankie J White
Department of Veterinary Integrative Biosciences, Texas A and M University, College Station, 77843, USA
Biol Reprod 73:1294-301. 2005..We conclude that, in pregnant pigs, SPP1 is induced by conceptus estrogen in uterine LE and is regulated in GE in a manner coincident with CL/placental progesterone production...
Ovine placental lactogen specifically binds to endometrial glands of the ovine uterusSekoni Noel
Center for Animal Biotechnology and Genomics and Department of Animal Science, Texas A and M University, College Station, Texas 77843-2471, USA
Biol Reprod 68:772-80. 2003..Thus, effects of oPL on the endometrial glands may be mediated by receptors for oPRL and oGH...
The sphingosine 1-phosphate (S1P) signaling pathway is regulated during pregnancy in sheepKathrin A Dunlap
Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, Texas
Biol Reprod 82:876-87. 2010..Thus, S1P signaling may be crucial for proper fetal-placental development...
Select nutrients in the ovine uterine lumen. VIII. Arginine stimulates proliferation of ovine trophectoderm cells through MTOR-RPS6K-RPS6 signaling cascade and synthesis of nitric oxide and polyaminesJin Young Kim
Center for Animal Biotechnology and Genomics, Department of Animal Science, Texas A and M University, College Station, Texas 77843 2471, USA
Biol Reprod 84:70-8. 2011..These results indicate that L-Arg enhances production of polyamines and NO and activates the MTOR/FRAP1-RPS6K-RPS6 signaling pathway to stimulate proliferation and migration of oTr cells...
Glycosylation dependent cell adhesion molecule 1-like protein and L-selectin expression in sheep interplacentomal and placentomal endometriumJésus J Muniz
Department of Veterinary Integrative Biosciences, Texas A and M University, College Station, Texas 77843, USA
Reproduction 131:751-61. 2006..These data suggest that GlyCAM-1 assumes distinct functions in compartment-specific regions of the sheep uterus...
Functional effects of transforming growth factor beta on adhesive properties of porcine trophectodermLaurie A Jaeger
Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, Texas 77843 4458, USA
Endocrinology 146:3933-42. 2005..These results suggest multiple effects of TGFbeta at the porcine conceptus-maternal interface, including integrin-mediated conceptus-maternal communication through LAP...
Analysis of osteopontin at the maternal-placental interface in pigsJane E Garlow
Center for Animal Biotechnology and Genomics, Department of Animal Science, Texas A and M University, College Station, Texas 77843-2471, USA
Biol Reprod 66:718-25. 2002..e., integrins) on conceptus and uterus to promote conceptus development and signaling between these tissues as key contributors to attachment and placentation in the pig...
Select nutrients in the ovine uterine lumen. I. Amino acids, glucose, and ions in uterine lumenal flushings of cyclic and pregnant ewesHaijun Gao
Center for Animal Biotechnology and Genomics, Departments of Animal Science and Veterinary Integrative Biosciences, Texas A and M University, College Station, Texas 77843, USA
Biol Reprod 80:86-93. 2009....
Progesterone and placental hormone actions on the uterus: insights from domestic animalsThomas E Spencer
Center for Animal Biotechnology and Genomics, Texas A and M University, College Station, TX 77843, USA
Biol Reprod 71:2-10. 2004..Strategic manipulation of these physiological mechanisms may offer therapeutic schemes to improve uterine capacity, conceptus survival, and reproductive health of domestic animals and humans...
Insulin-like growth factor II activates phosphatidylinositol 3-kinase-protooncogenic protein kinase 1 and mitogen-activated protein kinase cell Signaling pathways, and stimulates migration of ovine trophectoderm cellsJinYoung Kim
Center for Animal Biotechnology and Genomics, Department of Animal Science, Texas A and M University, College Station, Texas 77843 2471, USA
Endocrinology 149:3085-94. 2008..Collectively, these results support the hypothesis that IGF-II coordinately activates multiple cell signaling pathways critical to survival, growth, and differentiation of the ovine conceptus during early pregnancy...
Regulation of expression of fibroblast growth factor 7 in the pig uterus by progesterone and estradiolHakhyun Ka
Center for Animal Biotechnology and Genomics, Texas A and M University, College Station, Texas 77843, USA
Biol Reprod 77:172-80. 2007....
Novel pathways for implantation and establishment and maintenance of pregnancy in mammalsFuller W Bazer
Departments of Animal Science, Texas A and M Health Sciences Center, College Station, TX 77843, USA
Mol Hum Reprod 16:135-52. 2010..Understanding the roles of the variety of hormones, growth factors and endogenous retroviral proteins in uterine receptivity for implantation is essential to enhancing reproductive health and fertility in humans and domestic animals...
The temporal expression of osteopontin (SPP-1) in the rodent model of alcoholic steatohepatitis: a potential biomarkerAtrayee Banerjee
Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX, 77843-4467, USA
Toxicol Pathol 34:373-84. 2006..The data indicates that OPN is transcribed and translated within the biliary epithelium. These findings suggest a potential role of OPN as an early biomarker in predicting inflammatory liver diseases such as ASH...
Implantation mechanisms: insights from the sheepThomas E Spencer
Center for Animal Biotechnology and Genomics, Animal Science and Veterinary Anatomy and Public Health, Texas A and M University, College Station, Texas 77843, USA
Reproduction 128:657-68. 2004....
Polyamine synthesis from proline in the developing porcine placentaGuoyao Wu
Department of Animal Science, Texas A and M University, College Station, Texas 77843 2471, USA
Biol Reprod 72:842-50. 2005..Our novel findings provide a new base of information for future studies to define the role of proline in fetoplacental growth and development...
Glutamine synthesis in the developing porcine placentaJon T Self
Department of Animal Science, Texas A and M University, College Station, Texas 77843-2471, USA
Biol Reprod 70:1444-51. 2004....
A highly efficient method for porcine cloning by nuclear transfer using in vitro-matured oocytesShawn C Walker
Center for Animal Biotechnology and Genomics, Texas A and M University, College Station, Texas 77843-4458, USA
Cloning Stem Cells 4:105-12. 2002..The method described here can be undertaken by a single micromanipulator at a reasonable cost, and should facilitate the broad utilization of porcine cloning technology in transgenic and nontransgenic applications...
Role of the cytoskeleton in formation and maintenance of angiogenic sproutsKayla J Bayless
Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, College Station, TX 77843 1114, USA
J Vasc Res 48:369-85. 2011....
Roles of Stat1, Stat2, and interferon regulatory factor-9 (IRF-9) in interferon tau regulation of IRF-1M David Stewart
Center for Animal Biotechnology and Genomics, Department of Animal Science, Texas A and M University, College Station, Texas 77843, USA
Biol Reprod 66:393-400. 2002..Collectively, results indicate that IFNtau effect on IRF-1 expression is primarily regulated by tyrosine-phosphorylated Stat1alpha or Stat1beta dimers, whereas the decline of IRF-1 after 6 h of IFNtau treatment is regulated by IRF-9...
Molecular characterization and expression of porcine bone morphogenetic protein receptor-IB in the uterus of cyclic and pregnant giltsJong G Kim
U S Department of Agriculture, Agricultural Research Service, Roman L Hruska U S Meat Animal Research Center, Clay Center, Nebraska 68933 0166, USA
Biol Reprod 68:735-43. 2003....
Premature estrogen exposure alters endometrial gene expression to disrupt pregnancy in the pigJason W Ross
Department of Animal Science, Oklahoma State University, Stillwater, OK 74078, USA
Endocrinology 148:4761-73. 2007....
