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| Katrin HinrichsSummaryAffiliation: Texas A and M University Country: USA Publications
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Publications
Update on equine ICSI and cloningKatrin Hinrichs
Department of Veterinary Physiology and Pharmacology, Texas A and M University, 4466 TAMU, College Station, TX 77843 4466, USA
Theriogenology 64:535-41. 2005..Horse owners are beginning to realize the potential of cloning for salvaging valuable equine genetics that may otherwise be lost...
Assisted reproduction techniques in the horseKatrin Hinrichs
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843 4466, USA
Reprod Fertil Dev 25:80-93. 2012..Equine expanded blastocysts may be vitrified after collapsing them via micromanipulation, with normal pregnancy rates after warming and transfer. Many of these recently developed techniques are now in clinical use...- Evaluation of foal production following intracytoplasmic sperm injection and blastocyst culture of oocytes from ovaries collected immediately before euthanasia or after death of mares under field conditionsKatrin Hinrichs
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843, USA
J Am Vet Med Assoc 241:1070-4. 2012..To evaluate the efficiency of foal production following intracytoplasmic sperm injection (ICSI) and blastocyst culture of oocytes from mares that died or were euthanized under field conditions... - In vitro-produced equine embryos: production of foals after transfer, assessment by differential staining and effect of medium calcium concentrations during cultureK Hinrichs
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843, USA
Theriogenology 68:521-9. 2007..More work is needed to optimize culture systems for in vitro production of equine embryos... - Equine cloningKatrin Hinrichs
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843 4466, USA
Vet Clin North Am Equine Pract 22:857-66. 2006.... - Production of horse foals via direct injection of roscovitine-treated donor cells and activation by injection of sperm extractK Hinrichs
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, 77843 4466, USA
Reproduction 131:1063-72. 2006.... - Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycinK Hinrichs
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, Texas 77843, USA
Reproduction 134:319-25. 2007..One foal died of pneumonia 48 h post partum and one foal died at 1 week of age after complications during induction of anesthesia; the remaining seven foals are currently 10-14 months of age... - Chromatin configuration within the germinal vesicle of horse oocytes: changes post mortem and relationship to meiotic and developmental competenceK Hinrichs
Department of Veterinary, College of Veterinary Medicine, Texas A and M University, Texas 77843 4466, USA
Biol Reprod 72:1142-50. 2005..Time of oocyte recovery and duration of maturation significantly affect the rate of blastocyst development. Oocytes with Cp and Ex cumuli have similar developmental competence to the blastocyst stage... - In vitro production of equine embryos: state of the artK Hinrichs
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, Texas 77843 4466, USA
Reprod Domest Anim 45:3-8. 2010..Pregnancy rates after transfer of in vitro-produced blastocysts are similar to those for embryos recovered ex vivo... - In vitro fertilization of in vitro-matured equine oocytes: effect of maturation medium, duration of maturation, and sperm calcium ionophore treatment, and comparison with rates of fertilization in vivo after oviductal transferK Hinrichs
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843 4466, USA
Biol Reprod 67:256-62. 2002..Further work is needed to determine the optimum environment for sperm capacitation and IVF in the horse... - The equine oocyte: factors affecting meiotic and developmental competenceKatrin Hinrichs
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843 4466, USA
Mol Reprod Dev 77:651-61. 2010..Fertilization in vitro may be achieved using intracytoplasmic sperm injection; culture of injected oocytes in a medium with high glucose can yield over 30% blastocyst development... - Treatments resulting in pregnancy in nonovulating, hormone-treated oocyte recipient maresK Hinrichs
Section of Large Animal Clinical Sciences, Tufts University School of Veterinary Medicine, North Grafton, MA 01536, USA
Theriogenology 54:1285-93. 2000..One foal died at birth. Establishment and maintenance of pregnancy in these mares indicates that nonovulating, hormone-treated mares may offer an alternative to cyclic recipients in oocyte transfer programs... - Meiotic competence in horse oocytes: interactions among chromatin configuration, follicle size, cumulus morphology, and seasonK Hinrichs
Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
Biol Reprod 62:1402-8. 2000..Condensation of chromatin in oocytes in smaller apparently viable follicles, while associated with acquisition of meiotic competence, may represent a pre-atretic change... - Activation of equine nuclear transfer oocytes: methods and timing of treatment in relation to nuclear remodelingYoung-Ho Choi
Departments of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843-4466, USA
Biol Reprod 70:46-53. 2004..These results indicate that although nuclear remodeling occurs rapidly in equine cytoplasts, early activation does not improve embryonic development after reconstruction... - Effect of ovary storage and oocyte transport method on maturation rate of horse oocytesLinda B Love
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843, USA
Theriogenology 59:765-74. 2003..Use of 1 ml of equilibrated maturation medium in a 1 ml glass vial was associated with maturation equivalent to that for standard incubation... - Physical and clinicopathologic findings in foals derived by use of somatic cell nuclear transfer: 14 cases (2004-2008)Aime K Johnson
Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843, USA
J Am Vet Med Assoc 236:983-90. 2010..To describe the health status of foals derived by use of somatic cell nuclear transfer (NT) at a university laboratory... - Microinjection of mouse phospholipase C zeta complementary RNA into mare oocytes induces long-lasting intracellular calcium oscillations and embryonic developmentSylvia J Bedford-Guaus
Department of Clinical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY 14853, USA
Reprod Fertil Dev 20:875-83. 2008.... - Holding immature equine oocytes in the absence of meiotic inhibitors: effect on germinal vesicle chromatin and blastocyst development after intracytoplasmic sperm injectionY H Choi
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Science, Texas A and M University, College Station, TX 77843, USA
Theriogenology 66:955-63. 2006.... - Cloning companion animals (horses, cats, and dogs)Mark Westhusin
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843-4466, USA
Cloning Stem Cells 5:301-17. 2003 - Effects of gas conditions, time of medium change, and ratio of medium to embryo on in vitro development of horse oocytes fertilized by intracytoplasmic sperm injectionYoung-Ho Choi
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843-4466, USA
Theriogenology 59:1219-29. 2003..There were no significant differences among ratio treatments in rates of cleavage or development to blastocyst... - Effect of holding technique and culture drop size in individual or group culture on blastocyst development after ICSI of equine oocytes with low meiotic competenceY H Choi
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843, United States
Anim Reprod Sci 102:38-47. 2007..The greatest blastocyst rate (39%) was obtained with group culture in a 5microl droplet... - Effect of sperm extract injection volume, injection of PLCzeta cRNA, and tissue cell line on efficiency of equine nuclear transferYoung Ho Choi
College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843 4466, USA
Cloning Stem Cells 11:301-8. 2009..7 and 3.0%, respectively). A healthy foal was produced from a blastocyst originating from a metaphase I oocyte... - Suppression of meiosis by inhibitors of m-phase proteins in horse oocytes with low meiotic competenceKatrin Hinrichs
College of Veterinary Medicine, Texas A and M University, College Station 77843-4466, USA
Zygote 10:37-45. 2002.... - Assessment of canine oocyte viability after transportation and storage under different conditionsCarol Hanna
College of Veterinary Medicine, Department of Physiology and Pharmacology, Texas A and M University, College Station, TX, 77843 4466, USA
Anim Reprod Sci 105:451-6. 2008..Hepes buffered media increased viability rates for isolated oocytes transported at 37 degrees C compared to a similar medium buffered with sodium bicarbonate... - Recovery of mare oocytes on a fixed biweekly schedule, and resulting blastocyst formation after intracytoplasmic sperm injectionCandace C Jacobson
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, Texas 77843 4466, USA
Theriogenology 73:1116-26. 2010..As performed in these separate experiments, aspiration of immature follicles provided more blastocysts per aspiration session... - Blastocyst development in equine oocytes with low meiotic competence after suppression of meiosis with roscovitine prior to in vitro maturationY H Choi
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843-4466, USA
Zygote 14:1-8. 2006..These data indicate that equine oocytes with compact cumuli can be held in roscovitine before maturation without any harmful effect on blastocyst formation... - Blastocyst formation rates in vivo and in vitro of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injectionY H Choi
Department of Veterinary Physiology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843, USA
Biol Reprod 70:1231-8. 2004..While we identified an in vitro culture system that provided repeatable blastocyst development without coculture, this yielded only half the rate of development achieved in vivo... - Activation of cumulus-free equine oocytes: effect of maturation medium, calcium ionophore concentration and duration of cycloheximide exposureY H Choi
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843-4466, USA
Reproduction 122:177-83. 2001..The highest rate of activation (82%) was observed in oocytes matured in TCM-199, treated with 50 micromol calcium ionophore l(-1) and cultured with cycloheximide for 24 h... - Heat shock protein 70 gene expression in equine blastocysts after exposure of oocytes to high temperatures in vitro or in vivo after exercise of donor maresC J Mortensen
Department of Animal Science, College of Agriculture and Life Sciences, Texas A and M University, College Station, TX 77843 2471, USA
Theriogenology 74:374-83. 2010..Embryos produced in vitro (including controls) had increased levels of HSPA1A mRNA relative to 18S rRNA compared to in vivo-produced embryos, suggesting a response to environmental insult... - Production of nuclear transfer horse embryos by Piezo-driven injection of somatic cell nuclei and activation with stallion sperm cytosolic extractY H Choi
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843, USA
Biol Reprod 67:561-7. 2002..that direct nuclear injection using the Piezo drill is an efficient method for nuclear transfer in horse and cattle oocytes and that sperm extract can efficiently activate horse oocytes both parthenogenetically and after nuclear transfer.. - A viable foal obtained by equine somatic cell nuclear transfer using oocytes recovered from immature follicles of live maresYoung Ho Choi
College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, Texas, USA
Theriogenology 79:791-6.e1. 2013..In conclusion, this is apparently the first report of production of a viable cloned foal from oocytes collected from immature follicles of live mares, supporting the possibility of cloning using oocytes from selected mares... - Factors affecting developmental competence of equine oocytes after intracytoplasmic sperm injectionY H Choi
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843-4466, USA
Reproduction 127:187-94. 2004..These data indicate that developmental competence differs between Ex and Cp equine oocytes, and is differentially affected by the duration of maturation and by composition of embryo culture media... - Developmental competence in vivo and in vitro of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection with fresh or frozen-thawed spermatozoaY H Choi
Departments of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843-4466, USA
Reproduction 123:455-65. 2002..2 medium results in a similar cleavage rate but reduced number of cells compared with in vivo culture within the oviduct. Bovine oocytes may be useful as models for assessing sperm function in horses... - Effect of co-culture with theca interna on nuclear maturation of horse oocytes with low meiotic competence, and subsequent fusion and activation rates after nuclear transferYoung-Ho Choi
Department of Veterinary Physiology, College of Veterinary Medicine, Texas A and M University, College Station 77843-4466, USA
Theriogenology 57:1005-11. 2002..In vitro matured equine oocytes have the potential to develop into embryos after nuclear transfer; this is the first full report of production of cloned embryos in this species... - Equine blastocyst development after intracytoplasmic injection of sperm subjected to two freeze-thaw cyclesY H Choi
Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, TX 77843-4466, USA
Theriogenology 65:808-19. 2006..Non-motile spermatozoa from reprocessed semen may also achieve embryo development after ICSI. To our knowledge, this is the first report evaluating the ability of refrozen spermatozoa to produce embryos by ICSI in any species... - Relationships among oocyte-cumulus morphology, follicular atresia, initial chromatin configuration, and oocyte meiotic competence in the horseK Hinrichs
Section of Theriogenology, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
Biol Reprod 57:377-84. 1997..The association of follicle atresia with increased meiotic competence suggests that acquisition of meiotic competence is related to a loss of suppressive activity by the degenerating follicle... - Hyperactivation of stallion sperm is required for successful in vitro fertilization of equine oocytesL A McPartlin
Departments of Clinical Sciences and Biological and Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
Biol Reprod 81:199-206. 2009..Conversely, sperm incubated in capacitating conditions but not treated with procaine did not fertilize (0%). These results support the hypothesis that capacitation and hyperactivation are required for successful IVF in the equine... - Effects of stage of oestrous cycle and progesterone supplementation during culture on maturation of canine oocytes in vitroL A Willingham-Rocky
Department of Veterinary Physiology and Pharmacology, Texas A and M University, College of Veterinary Medicine, College Station, Texas 77843 4466, USA
Reproduction 126:501-8. 2003..However, stage of oestrous cycle is a key factor in the selection criteria for meiotically competent canine oocytes for use in in vitro experiments... - Description and genetic analysis of three sets of monozygotic twins resulting from transfers of single embryos to recipient maresSemira S Mancill
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843, USA
J Am Vet Med Assoc 238:1040-3. 2011..3 sets of monozygotic twins resulting from transfers of single embryos to recipient mares were examined... - Ejaculate and type of freezing extender affect rates of fertilization of horse oocytes in vitroL M Roasa
Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843, USA
Theriogenology 68:560-6. 2007..These factors may help to explain the great variability in fertilization rates reported with equine IVF, both among and within laboratories... - In vitro development of equine nuclear transfer embryos: effects of oocyte maturation media and amino acid composition during embryo cultureY H Choi
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station 77843-4466, USA
Zygote 11:77-86. 2003..CZB-C medium may be used for culture of equine embryos with results similar to those obtained with G1.2 medium, thus providing a base medium that may be modified for further study of culture requirements of equine embryos... - Transport of equine ovaries for assisted reproductionB I Ribeiro
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843 4466, USA
Anim Reprod Sci 108:171-9. 2008..Further work is needed to determine if there is a critical time before 7h post-mortem by which equine oocytes should be recovered to maximize developmental competence... - Effect of holding at room temperature on initial chromatin configuration and in vitro maturation rate of equine oocytesC C Love
Department of Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station 77843, USA
Theriogenology 57:1973-9. 2002..018)... - Embryo recovery from exercised maresC J Mortensen
Department of Animal Science, College of Agriculture and Life Sciences, Texas A and M University, College Station, TX 77843 2471, USA
Anim Reprod Sci 110:237-44. 2009..051). These data indicate that exercising mares in a hot and humid environment are associated with changes in ovarian follicle development and ovulation, and a reduction in embryo recovery... - Patterns of intracellular calcium oscillations in horse oocytes fertilized by intracytoplasmic sperm injection: possible explanations for the low success of this assisted reproduction technique in the horseSylvia J Bedford
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts 01003, USA
Biol Reprod 70:936-44. 2004..These findings may have implications to improve the success of ICSI in the equine and other livestock species... - Meiotic competence of equine oocytes and pronucleus formation after intracytoplasmic sperm injection (ICSI) as related to granulosa cell apoptosisMaria Elena Dell'Aquila
Department of Animal Production Section of Reproductive Biology and Veterinary Obstetrics, University of Bari, 70010 Valenzano, Bari, Italy
Biol Reprod 68:2065-72. 2003..These results provide selection criteria for horse oocytes used in assisted reproductive techniques so that embryo production may be maximized...