Research Topics
| Thomas FichtSummaryAffiliation: Texas A and M University Country: USA Publications
Research Grants
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Detail Information
Publications
Mariner mutagenesis of Brucella melitensis reveals genes with previously uncharacterized roles in virulence and survivalQingmin Wu
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
BMC Microbiol 6:102. 2006..melitensis genome. Conjugation was performed efficiently and rapidly in less than one generation in order to minimize the formation of siblings while assuring the highest level of genome coverage...- Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expressionJenni N Weeks
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843 4467, USA
BMC Microbiol 10:167. 2010..In an effort to further delineate the role of VjbR to virulence and survival, gene expression under the control of VjbR and AI was characterized using microarray analysis... 
In vitro mutagenesis of Brucella speciesThomas A Ficht
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station, TX, USA
Methods Mol Biol 634:15-35. 2010..Toward this goal, this chapter describes current approaches used to mutagenize Brucella spp...- Brucella taxonomy and evolutionThomas Ficht
Texas A and M University, Veterinary Pathobiology, TAMUs 4467, College Station, TX 77843, USA
Future Microbiol 5:859-66. 2010.... - Brucellosis: the case for live, attenuated vaccinesThomas A Ficht
Department of Veterinary Pathobiology, Texas A and M University and TX AgriLife Research, College Station, TX 77843 4467, USA
Vaccine 27:D40-3. 2009..Based upon preliminary data, it is postulated that such vaccine delivery systems can be storage stable, administered orally or intranasally, and generally applicable to a number of agents... - Immunization with a single dose of a microencapsulated Brucella melitensis mutant enhances protection against wild-type challengeAngela M Arenas-Gamboa
Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, TX 77845 1114, USA
Infect Immun 76:2448-55. 2008..Together, these results suggest that microencapsulation of live attenuated organisms offers the ability to increase the efficacy of vaccine candidates... - Enhanced immune response of red deer (Cervus elaphus) to live rb51 vaccine strain using composite microspheresAngela M Arenas-Gamboa
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, 4467 TAMU, College Station, Texas 77843 4467, USA
J Wildl Dis 45:165-73. 2009..These data suggest that alginate-VpB microspheres provide a method to enhance the RB51 vaccine performance in elk... 
Evaluation of novel Brucella melitensis unmarked deletion mutants for safety and efficacy in the goat model of brucellosisMelissa M Kahl-McDonagh
Department of Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, TX 77843 4467, United States
Vaccine 24:5169-77. 2006..Both strains were protective against abortion and against infection in the majority of pregnant goats, although BMDeltaasp24 was more efficacious than BMDeltavirB2 against challenge infection...- Differential requirements for VirB1 and VirB2 during Brucella abortus infectionAndreas B den Hartigh
Department of Medical Microbiology and Immunology, Texas A and M University Health Science Center, College Station, TX 77843-1114, USA
Infect Immun 72:5143-9. 2004..In contrast, the nonpolar virB1 mutant persisted at wild-type levels, showing that the function of VirB1 is dispensable in the mouse model of persistent infection... - Oral vaccination with microencapsuled strain 19 vaccine confers enhanced protection against Brucella abortus strain 2308 challenge in red deer (Cervus elaphus elaphus)Angela M Arenas-Gamboa
Department of Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, Texas 77845 1114, USA
J Wildl Dis 45:1021-9. 2009..This system is a potential improvement for efficacious Brucella-vaccine delivery to wildlife in the GYA... - Polymeric particles in vaccine deliveryAllison C Rice-Ficht
Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, College of Medicine, College Station, TX 77843 1114, USA
Curr Opin Microbiol 13:106-12. 2010..The size and dissolution rates of particles as well as surface chemistry and charge appear to be central in tuning adaptive immunity... - Intracellular survival of Brucella: defining the link with persistenceThomas A Ficht
Veterinary Pathobiology, Texas Agricultural Experiment Station, Texas A and M University, College Station, TX 77843 4467, USA
Vet Microbiol 92:213-23. 2003..The results suggest that Brucella utilizes a battery of metabolic functions to sustain itself in intracellular environments in conjunction with altering the intracellular course of infection... - Discovery of Brucella virulence mechanisms using mutational analysisThomas A Ficht
Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, TX 77843 4467, USA
Vet Microbiol 90:311-5. 2002..Direct and controlled mutagenesis of Brucella remains a valuable experimental approach to characterize the role of these genes in survival and virulence... - RNAi screen of endoplasmic reticulum-associated host factors reveals a role for IRE1alpha in supporting Brucella replicationQing Ming Qin
Department of Plant Pathology and Microbiology, Texas A and M University, College Station, Texas, United States of America
PLoS Pathog 4:e1000110. 2008..Finally, we propose a model in which IRE1alpha, and other ER-associated genes uncovered in our screen, mediate Brucella replication by promoting autophagosome biogenesis... - Evidence of Brucella abortus OPS dictating uptake and restricting NF-kappaB activation in murine macrophagesJianwu Pei
Department of Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, TX 77843 4467, USA
Microbes Infect 10:582-90. 2008..These results provide evidence that the presence of B. abortus OPS dictates the interactions between Brucella and specific cell surface receptors minimizing macrophage activation and enhancing Brucella survival and/or persistence... - Cytotoxicity in macrophages infected with rough Brucella mutants is type IV secretion system dependentJianwu Pei
Department of Veterinary Pathobiology, Texas A and M University, College Station, TX 77843 4467, USA
Infect Immun 76:30-7. 2008..Taken together, these results demonstrate that Brucella cytotoxicity in macrophages is T4SS dependent... - Brucella abortus rough mutants are cytopathic for macrophages in cultureJianwu Pei
Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, Texas 77843-4467, USA
Infect Immun 72:440-50. 2004..These findings suggest a role for O antigen during the early stages of host-agent interaction that is essential in establishing an intracellular niche that maintains and supports persistent intracellular infection resulting in disease... - Brucella abortus rough mutants induce macrophage oncosis that requires bacterial protein synthesis and direct interaction with the macrophageJianwu Pei
Department of Veterinary Pathobiology, Texas A and M University, College Station, TX 77843-4467, USA
Infect Immun 74:2667-75. 2006..In conclusion, Brucella rough mutant infection induces necrotic and oncotic macrophage cell death that requires bacterial protein synthesis and direct interaction of bacteria with the target cells... - Rapid and sensitive detection of Mycobacterium avium subsp. paratuberculosis in bovine milk and feces by a combination of immunomagnetic bead separation-conventional PCR and real-time PCRSangeeta Khare
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843-4467, USA
J Clin Microbiol 42:1075-81. 2004.... - Epidemiologic study of results of pulsed-field gel electrophoresis of isolates of Rhodococcus equi obtained from horses and horse farmsNoah D Cohen
Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843-4475, USA
Am J Vet Res 64:153-61. 2003..Only rarely will it be possible to link infections to a given site or region on the basis of analysis of isolates by use of PFGE of chromosomal DNA... - Virulence criteria for Brucella abortus strains as determined by interferon regulatory factor 1-deficient miceJinkyung Ko
Laboratory of Cellular and Molecular Immunology, Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison 53706, USA
Infect Immun 70:7004-12. 2002..Thus, IRF-1(-/-) mice are important to determining the level of Brucella virulence, to evaluating Brucella mutants for attenuation, and to investigating adaptive immunity in brucellosis... - Identification of the Brucella melitensis vaccine strain Rev.1 in animals and humans in Israel by PCR analysis of the PstI site polymorphism of its omp2 geneSvetlana Bardenstein
Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan 50250, Israel
J Clin Microbiol 40:1475-80. 2002..This method facilitates identification of the Rev.1 vaccine strain in both animals and humans in Israel... - Laboratory maintenance of Brucella abortusYao-Hui Sun
University of California at Davis, Davis, California, USA
Curr Protoc Microbiol . 2005..Furthermore, this organism is classified in the U.S. as a Select Agent, which restricts its use to laboratories registered with the U.S. government's Select Agent programs...
Research Grants
- Improved Brucella Vaccine StrainsThomas Ficht; Fiscal Year: 2003..Many of the gene products identified may serve as targets for new therapeutic regimens, but that is beyond the scope of the current proposal. ..
- Improved Brucella Vaccine StrainsThomas Ficht; Fiscal Year: 2009..abstract_text> ..