Thomas Ficht

Summary

Affiliation: Texas A and M University
Country: USA

Publications

  1. pmc Mariner mutagenesis of Brucella melitensis reveals genes with previously uncharacterized roles in virulence and survival
    Qingmin Wu
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
    BMC Microbiol 6:102. 2006
  2. pmc Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expression
    Jenni N Weeks
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843 4467, USA
    BMC Microbiol 10:167. 2010
  3. doi request reprint In vitro mutagenesis of Brucella species
    Thomas A Ficht
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station, TX, USA
    Methods Mol Biol 634:15-35. 2010
  4. pmc Brucella taxonomy and evolution
    Thomas Ficht
    Texas A and M University, Veterinary Pathobiology, TAMUs 4467, College Station, TX 77843, USA
    Future Microbiol 5:859-66. 2010
  5. pmc Brucellosis: the case for live, attenuated vaccines
    Thomas A Ficht
    Department of Veterinary Pathobiology, Texas A and M University and TX AgriLife Research, College Station, TX 77843 4467, USA
    Vaccine 27:D40-3. 2009
  6. pmc Immunization with a single dose of a microencapsulated Brucella melitensis mutant enhances protection against wild-type challenge
    Angela M Arenas-Gamboa
    Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, TX 77845 1114, USA
    Infect Immun 76:2448-55. 2008
  7. pmc Enhanced immune response of red deer (Cervus elaphus) to live rb51 vaccine strain using composite microspheres
    Angela M Arenas-Gamboa
    Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, 4467 TAMU, College Station, Texas 77843 4467, USA
    J Wildl Dis 45:165-73. 2009
  8. ncbi request reprint Evaluation of novel Brucella melitensis unmarked deletion mutants for safety and efficacy in the goat model of brucellosis
    Melissa M Kahl-McDonagh
    Department of Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, TX 77843 4467, United States
    Vaccine 24:5169-77. 2006
  9. pmc Differential requirements for VirB1 and VirB2 during Brucella abortus infection
    Andreas B den Hartigh
    Department of Medical Microbiology and Immunology, Texas A and M University Health Science Center, College Station, TX 77843 1114, USA
    Infect Immun 72:5143-9. 2004
  10. ncbi request reprint Oral vaccination with microencapsuled strain 19 vaccine confers enhanced protection against Brucella abortus strain 2308 challenge in red deer (Cervus elaphus elaphus)
    Angela M Arenas-Gamboa
    Department of Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, Texas 77845 1114, USA
    J Wildl Dis 45:1021-9. 2009

Research Grants

  1. Improved Brucella Vaccine Strains
    Thomas Ficht; Fiscal Year: 2003
  2. Improved Brucella Vaccine Strains
    Thomas Ficht; Fiscal Year: 2009

Collaborators

Detail Information

Publications23

  1. pmc Mariner mutagenesis of Brucella melitensis reveals genes with previously uncharacterized roles in virulence and survival
    Qingmin Wu
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
    BMC Microbiol 6:102. 2006
    ..melitensis genome. Conjugation was performed efficiently and rapidly in less than one generation in order to minimize the formation of siblings while assuring the highest level of genome coverage...
  2. pmc Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expression
    Jenni N Weeks
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843 4467, USA
    BMC Microbiol 10:167. 2010
    ..In an effort to further delineate the role of VjbR to virulence and survival, gene expression under the control of VjbR and AI was characterized using microarray analysis...
  3. doi request reprint In vitro mutagenesis of Brucella species
    Thomas A Ficht
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station, TX, USA
    Methods Mol Biol 634:15-35. 2010
    ..Toward this goal, this chapter describes current approaches used to mutagenize Brucella spp...
  4. pmc Brucella taxonomy and evolution
    Thomas Ficht
    Texas A and M University, Veterinary Pathobiology, TAMUs 4467, College Station, TX 77843, USA
    Future Microbiol 5:859-66. 2010
    ....
  5. pmc Brucellosis: the case for live, attenuated vaccines
    Thomas A Ficht
    Department of Veterinary Pathobiology, Texas A and M University and TX AgriLife Research, College Station, TX 77843 4467, USA
    Vaccine 27:D40-3. 2009
    ..Based upon preliminary data, it is postulated that such vaccine delivery systems can be storage stable, administered orally or intranasally, and generally applicable to a number of agents...
  6. pmc Immunization with a single dose of a microencapsulated Brucella melitensis mutant enhances protection against wild-type challenge
    Angela M Arenas-Gamboa
    Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, TX 77845 1114, USA
    Infect Immun 76:2448-55. 2008
    ..Together, these results suggest that microencapsulation of live attenuated organisms offers the ability to increase the efficacy of vaccine candidates...
  7. pmc Enhanced immune response of red deer (Cervus elaphus) to live rb51 vaccine strain using composite microspheres
    Angela M Arenas-Gamboa
    Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, 4467 TAMU, College Station, Texas 77843 4467, USA
    J Wildl Dis 45:165-73. 2009
    ..These data suggest that alginate-VpB microspheres provide a method to enhance the RB51 vaccine performance in elk...
  8. ncbi request reprint Evaluation of novel Brucella melitensis unmarked deletion mutants for safety and efficacy in the goat model of brucellosis
    Melissa M Kahl-McDonagh
    Department of Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, TX 77843 4467, United States
    Vaccine 24:5169-77. 2006
    ..Both strains were protective against abortion and against infection in the majority of pregnant goats, although BMDeltaasp24 was more efficacious than BMDeltavirB2 against challenge infection...
  9. pmc Differential requirements for VirB1 and VirB2 during Brucella abortus infection
    Andreas B den Hartigh
    Department of Medical Microbiology and Immunology, Texas A and M University Health Science Center, College Station, TX 77843 1114, USA
    Infect Immun 72:5143-9. 2004
    ..In contrast, the nonpolar virB1 mutant persisted at wild-type levels, showing that the function of VirB1 is dispensable in the mouse model of persistent infection...
  10. ncbi request reprint Oral vaccination with microencapsuled strain 19 vaccine confers enhanced protection against Brucella abortus strain 2308 challenge in red deer (Cervus elaphus elaphus)
    Angela M Arenas-Gamboa
    Department of Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, Texas 77845 1114, USA
    J Wildl Dis 45:1021-9. 2009
    ..This system is a potential improvement for efficacious Brucella-vaccine delivery to wildlife in the GYA...
  11. doi request reprint Polymeric particles in vaccine delivery
    Allison C Rice-Ficht
    Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, College of Medicine, College Station, TX 77843 1114, USA
    Curr Opin Microbiol 13:106-12. 2010
    ..The size and dissolution rates of particles as well as surface chemistry and charge appear to be central in tuning adaptive immunity...
  12. ncbi request reprint Intracellular survival of Brucella: defining the link with persistence
    Thomas A Ficht
    Veterinary Pathobiology, Texas Agricultural Experiment Station, Texas A and M University, College Station, TX 77843 4467, USA
    Vet Microbiol 92:213-23. 2003
    ..The results suggest that Brucella utilizes a battery of metabolic functions to sustain itself in intracellular environments in conjunction with altering the intracellular course of infection...
  13. ncbi request reprint Discovery of Brucella virulence mechanisms using mutational analysis
    Thomas A Ficht
    Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, TX 77843 4467, USA
    Vet Microbiol 90:311-5. 2002
    ..Direct and controlled mutagenesis of Brucella remains a valuable experimental approach to characterize the role of these genes in survival and virulence...
  14. pmc RNAi screen of endoplasmic reticulum-associated host factors reveals a role for IRE1alpha in supporting Brucella replication
    Qing Ming Qin
    Department of Plant Pathology and Microbiology, Texas A and M University, College Station, Texas, United States of America
    PLoS Pathog 4:e1000110. 2008
    ..Finally, we propose a model in which IRE1alpha, and other ER-associated genes uncovered in our screen, mediate Brucella replication by promoting autophagosome biogenesis...
  15. pmc Evidence of Brucella abortus OPS dictating uptake and restricting NF-kappaB activation in murine macrophages
    Jianwu Pei
    Department of Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, TX 77843 4467, USA
    Microbes Infect 10:582-90. 2008
    ..These results provide evidence that the presence of B. abortus OPS dictates the interactions between Brucella and specific cell surface receptors minimizing macrophage activation and enhancing Brucella survival and/or persistence...
  16. pmc Cytotoxicity in macrophages infected with rough Brucella mutants is type IV secretion system dependent
    Jianwu Pei
    Department of Veterinary Pathobiology, Texas A and M University, College Station, TX 77843 4467, USA
    Infect Immun 76:30-7. 2008
    ..Taken together, these results demonstrate that Brucella cytotoxicity in macrophages is T4SS dependent...
  17. pmc Brucella abortus rough mutants are cytopathic for macrophages in culture
    Jianwu Pei
    Veterinary Pathobiology, Texas A and M University and Texas Agricultural Experiment Station, College Station, Texas 77843 4467, USA
    Infect Immun 72:440-50. 2004
    ..These findings suggest a role for O antigen during the early stages of host-agent interaction that is essential in establishing an intracellular niche that maintains and supports persistent intracellular infection resulting in disease...
  18. pmc Brucella abortus rough mutants induce macrophage oncosis that requires bacterial protein synthesis and direct interaction with the macrophage
    Jianwu Pei
    Department of Veterinary Pathobiology, Texas A and M University, College Station, TX 77843 4467, USA
    Infect Immun 74:2667-75. 2006
    ..In conclusion, Brucella rough mutant infection induces necrotic and oncotic macrophage cell death that requires bacterial protein synthesis and direct interaction of bacteria with the target cells...
  19. pmc Rapid and sensitive detection of Mycobacterium avium subsp. paratuberculosis in bovine milk and feces by a combination of immunomagnetic bead separation-conventional PCR and real-time PCR
    Sangeeta Khare
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843 4467, USA
    J Clin Microbiol 42:1075-81. 2004
    ....
  20. ncbi request reprint Epidemiologic study of results of pulsed-field gel electrophoresis of isolates of Rhodococcus equi obtained from horses and horse farms
    Noah D Cohen
    Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843 4475, USA
    Am J Vet Res 64:153-61. 2003
    ..To compare isolates of Rhodococcus equi on the basis of geographic source and virulence status by use of pulsed-field gel electrophoresis (PFGE)...
  21. pmc Virulence criteria for Brucella abortus strains as determined by interferon regulatory factor 1-deficient mice
    Jinkyung Ko
    Laboratory of Cellular and Molecular Immunology, Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison 53706, USA
    Infect Immun 70:7004-12. 2002
    ..Thus, IRF-1(-/-) mice are important to determining the level of Brucella virulence, to evaluating Brucella mutants for attenuation, and to investigating adaptive immunity in brucellosis...
  22. pmc Identification of the Brucella melitensis vaccine strain Rev.1 in animals and humans in Israel by PCR analysis of the PstI site polymorphism of its omp2 gene
    Svetlana Bardenstein
    Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan 50250, Israel
    J Clin Microbiol 40:1475-80. 2002
    ..This method facilitates identification of the Rev.1 vaccine strain in both animals and humans in Israel...
  23. doi request reprint Laboratory maintenance of Brucella abortus
    Yao Hui Sun
    University of California at Davis, Davis, California, USA
    Curr Protoc Microbiol . 2005
    ..Furthermore, this organism is classified in the U.S. as a Select Agent, which restricts its use to laboratories registered with the U.S. government's Select Agent programs...

Research Grants8

  1. Improved Brucella Vaccine Strains
    Thomas Ficht; Fiscal Year: 2003
    ..Many of the gene products identified may serve as targets for new therapeutic regimens, but that is beyond the scope of the current proposal. ..
  2. Improved Brucella Vaccine Strains
    Thomas Ficht; Fiscal Year: 2009
    ..abstract_text> ..