Peter Christie

Summary

Affiliation: Texas Medical Center
Country: USA

Publications

  1. ncbi request reprint Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells
    P J Christie
    Dept of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Trends Microbiol 8:354-60. 2000
  2. ncbi request reprint Type IV secretion: intercellular transfer of macromolecules by systems ancestrally related to conjugation machines
    P J Christie
    Department of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Mol Microbiol 40:294-305. 2001
  3. ncbi request reprint Biogenesis, architecture, and function of bacterial type IV secretion systems
    Peter J Christie
    Department of Microbiology and Molecular Genetics, UT Houston Medical School, Houston, Texas 77030, USA
    Annu Rev Microbiol 59:451-85. 2005
  4. ncbi request reprint Structural and dynamic properties of bacterial type IV secretion systems (review)
    Peter J Christie
    Department of Microbiology and Molecular Genetics, UT Houston Medical School, Houston, Texas 77030, USA
    Mol Membr Biol 22:51-61. 2005
  5. ncbi request reprint Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systems
    Peter J Christie
    Department of Microbiology and Molecular Genetics, UT Houston Medical School, 6431 Fannin, JFB1 765, Houston TX 77030, USA
    Biochim Biophys Acta 1694:219-34. 2004
  6. doi request reprint Agrobacterium VirB10 domain requirements for type IV secretion and T pilus biogenesis
    Simon J Jakubowski
    University of Texas Houston Medical School, Department of Microbiology and Molecular Genetics, 6431 Fannin Street, Houston, TX 77030, USA
    Mol Microbiol 71:779-94. 2009
  7. pmc Agrobacterium VirB10, an ATP energy sensor required for type IV secretion
    Eric Cascales
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 101:17228-33. 2004
  8. ncbi request reprint Energetic components VirD4, VirB11 and VirB4 mediate early DNA transfer reactions required for bacterial type IV secretion
    Krishnamohan Atmakuri
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, JFB1 765, Houston, TX 77030, USA
    Mol Microbiol 54:1199-211. 2004
  9. ncbi request reprint Definition of a bacterial type IV secretion pathway for a DNA substrate
    Eric Cascales
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, JFB1 765, Houston, TX 77030, USA
    Science 304:1170-3. 2004
  10. ncbi request reprint VirE2, a type IV secretion substrate, interacts with the VirD4 transfer protein at cell poles of Agrobacterium tumefaciens
    Krishnamohan Atmakuri
    Department of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Mol Microbiol 49:1699-713. 2003

Research Grants

Collaborators

  • Eric Cascales
  • J P Vogel
  • Gabriel Waksman
  • Gary M Dunny
  • Andrew N Binns
  • W Margolin
  • Tracy Palmer
  • Simon J Jakubowski
  • Krishnamohan Atmakuri
  • Zhiyong Ding
  • Vidhya Krishnamoorthy
  • Jennifer E Kerr
  • Richard Bayliss
  • Cristina E Alvarez-Martinez
  • Yuqing Chen
  • Anastasias Economou
  • Ines Chen
  • Isaac Garza
  • Hye Jeong Yeo
  • Dawn Manias
  • Xiaolin Zhang
  • Richard Harris
  • Oliver T Burton
  • Amy Monier
  • Paul C Driscoll
  • Loic Coutte
  • Lois M Banta
  • Remi Fronzes
  • Anthony P Pugsley
  • Greg V Plano
  • Rachel C Fernandez
  • David Dubnau
  • Zhenming Zhao
  • Atmakuri Krishnamohan

Detail Information

Publications25

  1. ncbi request reprint Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells
    P J Christie
    Dept of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Trends Microbiol 8:354-60. 2000
    ..Other pathogens, including Bordetella pertussis, Legionella pneumophila, Brucellaspp. and Helicobacter pylori, use type IV machines to export effector proteins to the extracellular milieu or the mammalian cell cytosol...
  2. ncbi request reprint Type IV secretion: intercellular transfer of macromolecules by systems ancestrally related to conjugation machines
    P J Christie
    Department of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Mol Microbiol 40:294-305. 2001
    ..The list of putative type IV family members is increasing rapidly, suggesting that macromolecular transfer by these systems is a widespread phenomenon in nature...
  3. ncbi request reprint Biogenesis, architecture, and function of bacterial type IV secretion systems
    Peter J Christie
    Department of Microbiology and Molecular Genetics, UT Houston Medical School, Houston, Texas 77030, USA
    Annu Rev Microbiol 59:451-85. 2005
    ..We also summarize salient features of the increasingly studied effector translocator systems of mammalian pathogens...
  4. ncbi request reprint Structural and dynamic properties of bacterial type IV secretion systems (review)
    Peter J Christie
    Department of Microbiology and Molecular Genetics, UT Houston Medical School, Houston, Texas 77030, USA
    Mol Membr Biol 22:51-61. 2005
    ..Together, these recent findings describe the mechanics of type IV secretion in unprecedented detail...
  5. ncbi request reprint Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systems
    Peter J Christie
    Department of Microbiology and Molecular Genetics, UT Houston Medical School, 6431 Fannin, JFB1 765, Houston TX 77030, USA
    Biochim Biophys Acta 1694:219-34. 2004
    ..This review summarizes recent progress in our understanding of the mechanistic details of DNA trafficking with a focus on the paradigmatic Agrobacterium tumefaciens VirB/D4 T4SS and related conjugation systems...
  6. doi request reprint Agrobacterium VirB10 domain requirements for type IV secretion and T pilus biogenesis
    Simon J Jakubowski
    University of Texas Houston Medical School, Department of Microbiology and Molecular Genetics, 6431 Fannin Street, Houston, TX 77030, USA
    Mol Microbiol 71:779-94. 2009
    ..The data further support a model that distinct domains of VirB10 regulate formation of the secretion channel or the T pilus...
  7. pmc Agrobacterium VirB10, an ATP energy sensor required for type IV secretion
    Eric Cascales
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 101:17228-33. 2004
    ..tumefaciens cell envelope...
  8. ncbi request reprint Energetic components VirD4, VirB11 and VirB4 mediate early DNA transfer reactions required for bacterial type IV secretion
    Krishnamohan Atmakuri
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, JFB1 765, Houston, TX 77030, USA
    Mol Microbiol 54:1199-211. 2004
    ..Our findings define genetic requirements for DNA substrate binding and the early transfer reactions of a bacterial type IV translocation pathway...
  9. ncbi request reprint Definition of a bacterial type IV secretion pathway for a DNA substrate
    Eric Cascales
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, JFB1 765, Houston, TX 77030, USA
    Science 304:1170-3. 2004
    ..Our results define the translocation pathway for a DNA substrate through a bacterial conjugation machine, specifying the contributions of each subunit of the secretory apparatus to substrate passage...
  10. ncbi request reprint VirE2, a type IV secretion substrate, interacts with the VirD4 transfer protein at cell poles of Agrobacterium tumefaciens
    Krishnamohan Atmakuri
    Department of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Mol Microbiol 49:1699-713. 2003
    ..Together, our findings establish for the first time that a protein substrate of a type IV secretion system is recruited to a member of the coupling protein superfamily...
  11. pmc Enterococcus faecalis PcfC, a spatially localized substrate receptor for type IV secretion of the pCF10 transfer intermediate
    Yuqing Chen
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, Houston, TX 77030, USA
    J Bacteriol 190:3632-45. 2008
    ....
  12. pmc Agrobacterium tumefaciens VirB6 protein participates in formation of VirB7 and VirB9 complexes required for type IV secretion
    Simon J Jakubowski
    Department of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Bacteriol 185:2867-78. 2003
    ..Our findings support a model that VirB6 mediates formation of VirB7 and VirB9 complexes required for biogenesis of the T pilus and the secretion channel...
  13. pmc Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions
    Krishnamohan Atmakuri
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, Houston, TX 77030, USA
    EMBO J 26:2540-51. 2007
    ....
  14. pmc A novel cytology-based, two-hybrid screen for bacteria applied to protein-protein interaction studies of a type IV secretion system
    Zhiyong Ding
    Department of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Bacteriol 184:5572-82. 2002
    ..Together, our findings establish a proof-of-concept for the use of cell-location-specific proteins for studies of interactions among cytosolic and membrane proteins in diverse bacterial species...
  15. pmc Agrobacterium tumefaciens twin-arginine-dependent translocation is important for virulence, flagellation, and chemotaxis but not type IV secretion
    Zhiyong Ding
    Department of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    J Bacteriol 185:760-71. 2003
    ..Furthermore, this secretion pathway contributes to flagellar biogenesis and chemotactic responses but not to sensory perception of plant signals or the assembly of a type IV secretion system...
  16. pmc Agrobacterium tumefaciens VirB9, an outer-membrane-associated component of a type IV secretion system, regulates substrate selection and T-pilus biogenesis
    Simon J Jakubowski
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, Houston, TX 77030, USA
    J Bacteriol 187:3486-95. 2005
    ..Our findings, together with computer-based analyses, support a model in which distinct domains of VirB9 contribute to substrate selection and translocation, establishment of channel subunit contacts, and T-pilus biogenesis...
  17. ncbi request reprint The outs and ins of bacterial type IV secretion substrates
    Zhiyong Ding
    Department of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, Houston, TX 77030, USA
    Trends Microbiol 11:527-35. 2003
    ..Rapid progress has been made toward identification of type IV secretion substrates and the requirements for substrate recognition...
  18. ncbi request reprint The versatile bacterial type IV secretion systems
    Eric Cascales
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030, USA
    Nat Rev Microbiol 1:137-49. 2003
    ....
  19. pmc Agrobacterium tumefaciens oncogenic suppressors inhibit T-DNA and VirE2 protein substrate binding to the VirD4 coupling protein
    Eric Cascales
    Department of Microbiology and Molecular Genetics, University of Texas Houston, Medical School, Houston, TX 77030, USA
    Mol Microbiol 58:565-79. 2005
    ..Our findings establish that a competing plasmid substrate and a plasmid fertility inhibition factor act on a common target, the T4S receptor, to inhibit docking of DNA and protein substrates to the translocation apparatus...
  20. pmc Biological diversity of prokaryotic type IV secretion systems
    Cristina E Alvarez-Martinez
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, Houston, TX 77030, USA
    Microbiol Mol Biol Rev 73:775-808. 2009
    ....
  21. ncbi request reprint Agrobacterium tumefaciens VirB6 domains direct the ordered export of a DNA substrate through a type IV secretion System
    Simon J Jakubowski
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 77030, USA
    J Mol Biol 341:961-77. 2004
    ..Our findings support a model whereby the periplasmic loop P2 comprises a portion of the secretion channel and distinct domains of VirB6 participate in channel subunit interactions required for substrate passage to the cell exterior...
  22. pmc Evidence for VirB4-mediated dislocation of membrane-integrated VirB2 pilin during biogenesis of the Agrobacterium VirB/VirD4 type IV secretion system
    Jennifer E Kerr
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin St, Houston, TX 77030, USA
    J Bacteriol 192:4923-34. 2010
    ..The VirB11 ATPase functioned together with VirB4 to induce a structural change in the pilin that was detectable by MPB labeling, suggestive of a role for VirB11 as a modulator of VirB4 dislocase activity...
  23. pmc NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system
    Richard Bayliss
    Institute of Structural Molecular Biology, University College London Birkbeck, Malet Street, London WC1E 7HX, United Kingdom
    Proc Natl Acad Sci U S A 104:1673-8. 2007
    ..tumefaciens inserts in the membrane and protrudes extracellularly. This complex structure elucidates the molecular basis for the interaction between two essential components of a T4S system...
  24. ncbi request reprint Secretion by numbers: Protein traffic in prokaryotes
    Anastasias Economou
    Institute of Molecular Biology and Biotechnology, F O R T H and University of Crete, PO Box 1527, GR 711 10 Iraklio, Crete, Greece
    Mol Microbiol 62:308-19. 2006
    ....
  25. ncbi request reprint The ins and outs of DNA transfer in bacteria
    Ines Chen
    Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Science 310:1456-60. 2005
    ..All or nearly all the machine components involved in transformation and conjugation have been identified, and here we present models for their roles in DNA transport...

Research Grants14

  1. TRANSLOCATION OF DNA ACROSS THE AGROBACTERIUM ENVELOPE
    Peter Christie; Fiscal Year: 2007
    ..The proposed studies will develop a mechanistic understanding of type IV secretion in unprecedented detail. ..
  2. DNA Translocation Across the Agrobacterium Envelope
    Peter Christie; Fiscal Year: 2005
    ....
  3. TRANSLOCATION OF DNA ACROSS THE AGROBACTERIUM ENVELOPE
    Peter Christie; Fiscal Year: 2001
    ....
  4. TRANSLOCATION OF DNA ACROSS THE AGROBACTERIUM ENVELOPE
    Peter J Christie; Fiscal Year: 2010
    ..The proposed studies will develop a mechanistic understanding of type IV secretion in unprecedented detail. ..