Robert S Chapkin


Affiliation: Texas A and M University
Country: USA


  1. Hou T, Davidson L, Kim E, Fan Y, Fuentes N, Triff K, et al. Nutrient-Gene Interaction in Colon Cancer, from the Membrane to Cellular Physiology. Annu Rev Nutr. 2016;36:543-70 pubmed publisher
    ..Finally, we address future challenges to the field regarding the identification of additional molecular mechanisms and other bioactive dietary molecules that can be utilized in our fight to reduce the incidence of colorectal cancer. ..
  2. Erazo Oliveras A, Fuentes N, Wright R, Chapkin R. Functional link between plasma membrane spatiotemporal dynamics, cancer biology, and dietary membrane-altering agents. Cancer Metastasis Rev. 2018;37:519-544 pubmed publisher
  3. Hou T, McMurray D, Chapkin R. Omega-3 fatty acids, lipid rafts, and T cell signaling. Eur J Pharmacol. 2016;785:2-9 pubmed publisher
    ..An understanding of these underlying mechanisms will provide a rationale for the use of n-3 PUFA in the treatment of chronic inflammation. ..
  4. Chapkin R, McMurray D, Davidson L, Patil B, Fan Y, Lupton J. Bioactive dietary long-chain fatty acids: emerging mechanisms of action. Br J Nutr. 2008;100:1152-7 pubmed publisher
  5. Fuentes N, Kim E, Fan Y, Chapkin R. Omega-3 fatty acids, membrane remodeling and cancer prevention. Mol Aspects Med. 2018;64:79-91 pubmed publisher
    ..Particular focus is placed on the importance of intrinsic lipid/cholesterol biosynthesis and metabolism and extrinsic dietary modification in cancer and its effect on plasma membrane properties. ..
  6. Shah M, Kim E, Davidson L, Knight J, Zoh R, Goldsby J, et al. Comparative effects of diet and carcinogen on microRNA expression in the stem cell niche of the mouse colonic crypt. Biochim Biophys Acta. 2016;1862:121-34 pubmed publisher
    ..To our knowledge, this is the first study to utilize Lgr5(+) reporter mice to determine the impact of diet and carcinogen on miRNA expression in colonic stem cells and their progeny. ..
  7. Hou T, Barhoumi R, Fan Y, Rivera G, Hannoush R, McMurray D, et al. n-3 polyunsaturated fatty acids suppress CD4(+) T cell proliferation by altering phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] organization. Biochim Biophys Acta. 2016;1858:85-96 pubmed publisher
    ..These data demonstrate that n-3 PUFA suppress T cell proliferation by altering plasma membrane topography and the spatial organization of PI(4,5)P2. ..
  8. Declercq V, McMurray D, Chapkin R. Obesity promotes colonic stem cell expansion during cancer initiation. Cancer Lett. 2015;369:336-43 pubmed publisher
    ..These results suggest that the responsiveness of colonic stem cells to adiponectin in diet-induced obesity is impaired and may contribute to the stem cell accumulation observed in obesity. ..
  9. Fan Y, Davidson L, Callaway E, Wright G, Safe S, Chapkin R. A bioassay to measure energy metabolism in mouse colonic crypts, organoids, and sorted stem cells. Am J Physiol Gastrointest Liver Physiol. 2015;309:G1-9 pubmed publisher
    ..We propose that our innovative methodology may facilitate future in vivo/ex vivo metabolic studies using environmental agents affecting colonocyte energy metabolism. ..

More Information


  1. Davidson L, Callaway E, Kim E, Weeks B, Fan Y, Allred C, et al. Targeted Deletion of p53 in Lgr5-Expressing Intestinal Stem Cells Promotes Colon Tumorigenesis in a Preclinical Model of Colitis-Associated Cancer. Cancer Res. 2015;75:5392-7 pubmed publisher
    ..Furthermore, we propose that stem cell targeting approaches are valuable for interrogating prevention and therapeutic strategies that aim to specifically eradicate genetically compromised stem cells. ..
  2. Kim E, Davidson L, Zoh R, Hensel M, Patil B, Jayaprakasha G, et al. Homeostatic responses of colonic LGR5+ stem cells following acute in vivo exposure to a genotoxic carcinogen. Carcinogenesis. 2016;37:206-14 pubmed publisher
    ..These findings highlight the mechanisms by which colonic Lgr5(+) stem cells respond to cancer-causing environmental factors. ..
  3. Fuentes N, Mlih M, Barhoumi R, Fan Y, Hardin P, Steele T, et al. Long-Chain n-3 Fatty Acids Attenuate Oncogenic KRas-Driven Proliferation by Altering Plasma Membrane Nanoscale Proteolipid Composition. Cancer Res. 2018;78:3899-3912 pubmed publisher
    ..b>Graphical Abstract: Cancer Res; 78(14); 3899-912. ©2018 AACR. ..
  4. Kim S, Neuendorff N, Chapkin R, Earnest D. Role of Inflammatory Signaling in the Differential Effects of Saturated and Poly-unsaturated Fatty Acids on Peripheral Circadian Clocks. EBioMedicine. 2016;7:100-11 pubmed publisher
  5. Knight J, Kim E, Ivanov I, Davidson L, Goldsby J, Hullar M, et al. Comprehensive site-specific whole genome profiling of stromal and epithelial colonic gene signatures in human sigmoid colon and rectal tissue. Physiol Genomics. 2016;48:651-9 pubmed publisher
    ..These findings provide strong impetus for considering colorectal tissue subtypes and location in future observational studies and clinical trials designed to evaluate the effects of exposures on colonic health. ..
  6. Fan Y, Davidson L, Chapkin R. Murine Colonic Organoid Culture System and Downstream Assay Applications. Methods Mol Biol. 2016;: pubmed
    ..Specific culture strategies including growth factor enriched Matrigel and Wnt and R-spondin conditioned media serve as key factors for enhancing the growth and cost efficiency of colonic organoid cultures. ..
  7. Navarro S, Neuhouser M, Cheng T, Tinker L, Shikany J, Snetselaar L, et al. The Interaction between Dietary Fiber and Fat and Risk of Colorectal Cancer in the Women's Health Initiative. Nutrients. 2016;8: pubmed
  8. Triff K, McLean M, Konganti K, Pang J, Callaway E, Zhou B, et al. Assessment of histone tail modifications and transcriptional profiling during colon cancer progression reveals a global decrease in H3K4me3 activity. Biochim Biophys Acta Mol Basis Dis. 2017;1863:1392-1402 pubmed publisher
    ..Collectively, our results indicate that carcinogen-induced changes in gene K4me3 DERs are harbingers of future transcriptional events, which drive malignant transformation of the colon. ..
  9. Fuentes N, Salinas M, Kim E, Chapkin R. Emerging role of chemoprotective agents in the dynamic shaping of plasma membrane organization. Biochim Biophys Acta Biomembr. 2017;1859:1668-1678 pubmed publisher
    ..This article is part of a Special Issue entitled: Membrane Lipid Therapy: Drugs Targeting Biomembranes edited by Pablo V. Escribá. ..
  10. Fan Y, Callaway E, M Monk J, S Goldsby J, Yang P, Vincent L, et al. A New Model to Study the Role of Arachidonic Acid in Colon Cancer Pathophysiology. Cancer Prev Res (Phila). 2016;9:750-7 pubmed publisher
    ..6%) expands the utility of the Fads1-null mouse model for long-term cancer prevention studies and (ii) that AA content in the colonic epithelium modulates colon cancer risk. Cancer Prev Res; 9(9); 750-7. ©2016 AACR. ..
  11. Seidel D, Azcarate Peril M, Chapkin R, Turner N. Shaping functional gut microbiota using dietary bioactives to reduce colon cancer risk. Semin Cancer Biol. 2017;46:191-204 pubmed publisher
  12. Declercq V, Goldsby J, McMurray D, Chapkin R. Distinct Adipose Depots from Mice Differentially Respond to a High-Fat, High-Salt Diet. J Nutr. 2016;146:1189-96 pubmed publisher
  13. Allen M, Fan Y, Monk J, Hou T, Barhoumi R, McMurray D, et al. n-3 PUFAs reduce T-helper 17 cell differentiation by decreasing responsiveness to interleukin-6 in isolated mouse splenic CD4? T cells. J Nutr. 2014;144:1306-13 pubmed publisher
    ..Our results suggest that n-3 PUFAs suppress Th17 cell differentiation in part by reducing membrane raft-dependent responsiveness to IL-6, an essential polarizing cytokine. ..