William E Brownell

Summary

Affiliation: Texas Medical Center
Country: USA

Publications

  1. ncbi request reprint Micro- and nanomechanics of the cochlear outer hair cell
    W E Brownell
    Bobby R Alford Department of Otorhinolaryngology and Communicative Sciences, Baylor College of Medicine and Department of Bioengineering, Rice University, Houston, Texas 77030, USA
    Annu Rev Biomed Eng 3:169-94. 2001
  2. pmc Membrane cholesterol modulates cochlear electromechanics
    William E Brownell
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
    Pflugers Arch 461:677-86. 2011
  3. pmc Cell membrane tethers generate mechanical force in response to electrical stimulation
    William E Brownell
    Bobby R Alford Department of Otolaryngology, Head and Neck Surgery, and Department of Neuroscience, Baylor College of Medicine, Houston, Texas, USA
    Biophys J 99:845-52. 2010
  4. pmc Poking versus deflection: anisotropy in action
    William E Brownell
    Department of Otolaryngology, Baylor College of Medicine, Houston, Texas, USA
    Biophys J 94:4157-8. 2008
  5. pmc Glycosylation regulates prestin cellular activity
    Lavanya Rajagopalan
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    J Assoc Res Otolaryngol 11:39-51. 2010
  6. pmc Membrane composition modulates prestin-associated charge movement
    John Sfondouris
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 283:22473-81. 2008
  7. pmc Tuning of the outer hair cell motor by membrane cholesterol
    Lavanya Rajagopalan
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Huffington Center on Aging and Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 282:36659-70. 2007
  8. pmc Prestin modulates mechanics and electromechanical force of the plasma membrane
    Rui Zhang
    Applied Physics Graduate Program, Rice University, Houston, Texas, USA
    Biophys J 93:L07-9. 2007
  9. pmc Effects of chlorpromazine on mechanical properties of the outer hair cell plasma membrane
    David R Murdock
    Department of Bioengineering, Rice University, Houston, Texas 77251 1892, USA
    Biophys J 89:4090-5. 2005
  10. ncbi request reprint Studies of plasma membrane mechanics and plasma membrane-cytoskeleton interactions using optical tweezers and fluorescence imaging
    Sergey A Ermilov
    Department of Bioengineering, Rice University, P O Box 1892, MS 142, Houston, TX 77251 1892, USA
    J Biomech 40:476-80. 2007

Research Grants

  1. MECHANICS OF THE COCHLEAR OUTER HAIR CELL
    WILLIAM BROWNELL; Fiscal Year: 2005
  2. Electromechanics of the Cochlear Outer Hair Cell
    WILLIAM BROWNELL; Fiscal Year: 2007
  3. INTRACOCHLEAR ELECTROCHEMICAL GRADIENTS
    WILLIAM BROWNELL; Fiscal Year: 2007
  4. Research Training in Otolaryngology Head & Neck Surgery
    WILLIAM BROWNELL; Fiscal Year: 2007
  5. INTRACOCHLEAR ELECTROCHEMICAL GRADIENTS
    WILLIAM BROWNELL; Fiscal Year: 2009
  6. INTRACOCHLEAR ELECTROCHEMICAL GRADIENTS
    Alexander A Spector; Fiscal Year: 2010
  7. MECHANICS OF THE COCHLEAR OUTER HAIR CELL
    WILLIAM BROWNELL; Fiscal Year: 2000
  8. INTRACOCHLEAR ELECTROCHEMICAL GRADIENTS
    WILLIAM BROWNELL; Fiscal Year: 2002
  9. Electromechanics of the Cochlear Outer Hair Cell
    William E Brownell; Fiscal Year: 2011

Collaborators

Detail Information

Publications30

  1. ncbi request reprint Micro- and nanomechanics of the cochlear outer hair cell
    W E Brownell
    Bobby R Alford Department of Otorhinolaryngology and Communicative Sciences, Baylor College of Medicine and Department of Bioengineering, Rice University, Houston, Texas 77030, USA
    Annu Rev Biomed Eng 3:169-94. 2001
    ..The methods and techniques utilized to increase our understanding of the central role outer hair cell mechanics plays in hearing are also relevant to broader research questions in cell mechanics, cell motility, and cell transduction...
  2. pmc Membrane cholesterol modulates cochlear electromechanics
    William E Brownell
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
    Pflugers Arch 461:677-86. 2011
    ..The enhanced electromechanical response reflects an increase in outer hair cell electromotility and may reveal features of cholesterol distribution and trafficking in outer hair cells...
  3. pmc Cell membrane tethers generate mechanical force in response to electrical stimulation
    William E Brownell
    Bobby R Alford Department of Otolaryngology, Head and Neck Surgery, and Department of Neuroscience, Baylor College of Medicine, Houston, Texas, USA
    Biophys J 99:845-52. 2010
    ..By harnessing the energy in the transmembrane electric field, cell membranes may contribute to processes as diverse as outer hair cell electromotility, ion channel gating, and transport...
  4. pmc Poking versus deflection: anisotropy in action
    William E Brownell
    Department of Otolaryngology, Baylor College of Medicine, Houston, Texas, USA
    Biophys J 94:4157-8. 2008
  5. pmc Glycosylation regulates prestin cellular activity
    Lavanya Rajagopalan
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    J Assoc Res Otolaryngol 11:39-51. 2010
    ..We speculate that the cholesterol regulation of prestin occurs through localization to and internalization from membrane microdomains by clathrin- and caveolin-dependent mechanisms...
  6. pmc Membrane composition modulates prestin-associated charge movement
    John Sfondouris
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 283:22473-81. 2008
    ..Our results quantify the relation between membrane cholesterol concentration and prestin-associated charge movement and enhance our understanding of how membrane composition modulates prestin function...
  7. pmc Tuning of the outer hair cell motor by membrane cholesterol
    Lavanya Rajagopalan
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Huffington Center on Aging and Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 282:36659-70. 2007
    ..These findings indicate that alterations in membrane cholesterol affect prestin function and functionally tune the outer hair cell...
  8. pmc Prestin modulates mechanics and electromechanical force of the plasma membrane
    Rui Zhang
    Applied Physics Graduate Program, Rice University, Houston, Texas, USA
    Biophys J 93:L07-9. 2007
    ..These results suggest that prestin-associated charge transfer is necessary for maximal EMF generation by the membrane...
  9. pmc Effects of chlorpromazine on mechanical properties of the outer hair cell plasma membrane
    David R Murdock
    Department of Bioengineering, Rice University, Houston, Texas 77251 1892, USA
    Biophys J 89:4090-5. 2005
    ..The CPZ-induced changes in tether viscoelastic behavior suggest that alterations in the mechanical properties of the OHC lateral wall could play a role in the modulation of OHC electromotility by CPZ...
  10. ncbi request reprint Studies of plasma membrane mechanics and plasma membrane-cytoskeleton interactions using optical tweezers and fluorescence imaging
    Sergey A Ermilov
    Department of Bioengineering, Rice University, P O Box 1892, MS 142, Houston, TX 77251 1892, USA
    J Biomech 40:476-80. 2007
    ....
  11. ncbi request reprint Effects of salicylate on plasma membrane mechanics
    Sergey A Ermilov
    Department of Bioengineering, Rice University, P O Box 1892, MS 142, Houston, Texas 77251 1892, USA
    J Neurophysiol 94:2105-10. 2005
    ....
  12. pmc Measuring forces at the leading edge: a force assay for cell motility
    Brenda Farrell
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
    Integr Biol (Camb) 5:204-14. 2013
    ..This demonstrates that actin kinetics can be monitored in a living cell by measuring force at the membrane, and used to probe the mobility of cells including cancer cells...
  13. pmc Tonotopic relationships reveal the charge density varies along the lateral wall of outer hair cells
    Christian Corbitt
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, Texas, USA
    Biophys J 102:2715-24. 2012
    ..3/A(LW) and ρ(LW) = 9/A(LW)). This shows contrary to conventional wisdom that σ(LW) and ρ(LW) are not constant along the length of an individual outer hair cell...
  14. ncbi request reprint Standing wave total internal reflection fluorescence microscopy to measure the size of nanostructures in living cells
    Olga Gliko
    Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biomed Opt 11:064013. 2006
    ..The combination of SWFM with total internal reflection (TIR), referred to as SW-TIRFM, allows additional improvement of axial resolution by selective excitation of fluorescence in a layer of about 100 nm...
  15. pmc Hypotonic swelling of salicylate-treated cochlear outer hair cells
    Man Zhi
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, United States
    Hear Res 228:95-104. 2007
    ....
  16. ncbi request reprint Fast two-dimensional standing-wave total-internal-reflection fluorescence microscopy using acousto-optic deflectors
    Olga Gliko
    Department of Otorhinolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
    Opt Lett 34:836-8. 2009
    ..This technique can provide real-time imaging of subresolution structures in live biological specimens near a glass-water interface...
  17. ncbi request reprint Excess plasma membrane and effects of ionic amphipaths on mechanics of outer hair cell lateral wall
    Noriko Morimoto
    Department of Otorhinolaryngology and Communicative Science, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Physiol Cell Physiol 282:C1076-86. 2002
    ..Application of CPZ and/or Sal decreased this strain-induced hydraulic conductivity. The impact of ionic amphipaths on OHC plasma membrane and lateral wall mechanics may contribute to their effects on OHC electromotility and hearing...
  18. pmc Dyslipidemia and auditory function
    M Bradley Evans
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, Texas 77030, USA
    Otol Neurotol 27:609-14. 2006
    ..These results suggest that whereas chronic dyslipidemia associated with elevated triglycerides may reduce auditory function, short-term dietary changes may not...
  19. pmc Photometric recording of transmembrane potential in outer hair cells
    Takashi Nakagawa
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
    J Neural Eng 3:79-86. 2006
    ..Results are consistent with the hypothesis that OHCs exhibit inhomogeneous membrane potentials that vary with position in analogy with the voltage in nerve axons...
  20. pmc Functional expression and microdomain localization of prestin in cultured cells
    Angela K Sturm
    Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
    Otolaryngol Head Neck Surg 136:434-9. 2007
    ..A model system to study prestin employs its transfection into cultured HEK 293 cells. Our goal was to characterize prestin's trafficking pathway and localization in the plasma membrane...
  21. pmc Compartmentalization of the outer hair cell demonstrated by slow diffusion in the extracisternal space
    Olga Gliko
    Department of Otorhinolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, Texas, USA
    Biophys J 97:1215-24. 2009
    ..Compared with free solution, the diffusion of 10 kDa dextran was slowed down in both the ECiS and the axial core by a factor of 4.6 and 1.6, respectively...
  22. pmc Voltage-dependent capacitance of human embryonic kidney cells
    Brenda Farrell
    Department of Otolaryngology and Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 73:041930. 2006
    ..We suggest that the capacitance-voltage relationships should be similar to that observed for HEK cells for OHCs that do not express prestin in their membranes...
  23. pmc Outer hair cell piezoelectricity: frequency response enhancement and resonance behavior
    Erik K Weitzel
    Bobby R Alford Department of Otorhinolaryngology and Communicative Sciences, Baylor College of Medicine, Houston, Texas 77030, USA
    J Acoust Soc Am 114:1462-6. 2003
    ..It is also possible that members of some mammalian orders use outer hair cell piezoelectric resonance in detecting species-specific vocalizations...
  24. pmc Identification of functionally important residues/domains in membrane proteins using an evolutionary approach coupled with systematic mutational analysis
    Lavanya Rajagopalan
    Bobby R Alford Department of Otolaryngology HNS, Baylor College of Medicine, Houston, TX, USA
    Methods Mol Biol 493:287-97. 2009
    ....
  25. pmc Efferent modulation of hair cell function
    Richard D Rabbitt
    Department of Bioengineering, University of Utah, Salt Lake City, Utah, USA
    Curr Opin Otolaryngol Head Neck Surg 19:376-81. 2011
    ..This review covers the articles published between 2010 and early 2011 that presented new findings on inner-ear efferents and their ability to modulate hair cell function...
  26. pmc Membrane tether formation from outer hair cells with optical tweezers
    Zhiwei Li
    Department of Bioengineering, Rice University, Houston, Texas 77251, USA
    Biophys J 82:1386-95. 2002
    ..The information these studies provide on the mechanical properties of the OHC lateral wall is important for understanding the mechanism of OHC electromotility...
  27. pmc Urocortin-deficient mice display normal stress-induced anxiety behavior and autonomic control but an impaired acoustic startle response
    Xiaozhong Wang
    Department of Molecular and Human Genetics, Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 22:6605-10. 2002
    ..Ucn may modulate the acoustic startle response through the Ucn-expressing neuron projections from the region of the Edinger-Westphal nucleus...
  28. ncbi request reprint Outer hair cell active force generation in the cochlear environment
    Zhijie Liao
    Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Acoust Soc Am 122:2215-25. 2007
    ..Our findings can provide a better understanding of the outer hair cell's role in the cochlear amplifier...
  29. ncbi request reprint Mechanosensitive channels in the lateral wall can enhance the cochlear outer hair cell frequency response
    Alexander A Spector
    Department of Biomedical Engineering, Johns Hopkins University, 720 Rutland Avenue, Traylor 411, Baltimore, MD 21205, USA
    Ann Biomed Eng 33:991-1002. 2005
    ..The effect of the channels is especially significant in an intermediate range of sound frequencies, and the channel-related gain is up to 3-4 times between 3 and 15 kHz...
  30. ncbi request reprint Effects of prestin on membrane mechanics and electromechanics
    Bahman Anvari
    Department of Bioengineering, University of California, Riverside, CA 92521, USA
    Conf Proc IEEE Eng Med Biol Soc 2007:5384-6. 2007
    ..We propose that prestin and membrane work in synergy to produce the electrical and mechanical changes that are required during OHC electromotility...

Research Grants35

  1. MECHANICS OF THE COCHLEAR OUTER HAIR CELL
    WILLIAM BROWNELL; Fiscal Year: 2005
    ..The model will allow us to predict force-deformation relationships for the OHC at acoustic frequencies and it will meet all the criteria for the mechanism of OHC force production. ..
  2. Electromechanics of the Cochlear Outer Hair Cell
    WILLIAM BROWNELL; Fiscal Year: 2007
    ..The final result will be a complete description of OHC electromechanical force production and its role in hearing. ..
  3. INTRACOCHLEAR ELECTROCHEMICAL GRADIENTS
    WILLIAM BROWNELL; Fiscal Year: 2007
    ..Clarification of the physical principles underlying electromotility will also contribute to the emerging field of biological nanotechnology. ..
  4. Research Training in Otolaryngology Head & Neck Surgery
    WILLIAM BROWNELL; Fiscal Year: 2007
    ....
  5. INTRACOCHLEAR ELECTROCHEMICAL GRADIENTS
    WILLIAM BROWNELL; Fiscal Year: 2009
    ..In addition to potential benefits to the hearing impaired, elucidation of the physical principles underlying the outer hair cell motor will contribute to the emerging field of biological nanotechnology. ..
  6. INTRACOCHLEAR ELECTROCHEMICAL GRADIENTS
    Alexander A Spector; Fiscal Year: 2010
    ..In addition to potential benefits to the hearing impaired, elucidation of the physical principles underlying the outer hair cell motor will contribute to the emerging field of biological nanotechnology. ..
  7. MECHANICS OF THE COCHLEAR OUTER HAIR CELL
    WILLIAM BROWNELL; Fiscal Year: 2000
    ..Knowledge of the microstructural elements and their functional significance in OHC mechanics will enhance our understanding of how the OHC contributes to mammalian hearing in health and disease. ..
  8. INTRACOCHLEAR ELECTROCHEMICAL GRADIENTS
    WILLIAM BROWNELL; Fiscal Year: 2002
    ..Clarification of the physical principles underlying electromotility will also contribute to the emerging field of biological nanotechnology. ..
  9. Electromechanics of the Cochlear Outer Hair Cell
    William E Brownell; Fiscal Year: 2011
    ..The final result will be a complete description of OHC electromechanical force production and its role in hearing. ..