Michael R Blackburn

Summary

Affiliation: Texas Medical Center
Country: USA

Publications

  1. doi request reprint Hypoxia-induced deoxycytidine kinase expression contributes to apoptosis in chronic lung disease
    Tingting Weng
    1University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    FASEB J 27:2013-26. 2013
  2. pmc Metabolic consequences of adenosine deaminase deficiency in mice are associated with defects in alveogenesis, pulmonary inflammation, and airway obstruction
    M R Blackburn
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston Medical School, Houston, Texas 77030, USA
    J Exp Med 192:159-70. 2000
  3. ncbi request reprint A role for neural pathways in adenosine-induced bronchoconstriction
    Michael R Blackburn
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Am J Physiol Lung Cell Mol Physiol 293:L22-4. 2007
  4. ncbi request reprint The use of enzyme therapy to regulate the metabolic and phenotypic consequences of adenosine deaminase deficiency in mice. Differential impact on pulmonary and immunologic abnormalities
    M R Blackburn
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 275:32114-21. 2000
  5. ncbi request reprint Too much of a good thing: adenosine overload in adenosine-deaminase-deficient mice
    Michael R Blackburn
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Houston, TX 77030, USA
    Trends Pharmacol Sci 24:66-70. 2003
  6. ncbi request reprint Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice
    Amir Mohsenin
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, University of Texas, Houston, Texas 77030, USA
    Am J Physiol Lung Cell Mol Physiol 293:L753-61. 2007
  7. pmc Increased adenosine contributes to penile fibrosis, a dangerous feature of priapism, via A2B adenosine receptor signaling
    Jiaming Wen
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, 6431 Fannin St, MSB 6 200, Houston, TX 77030, USA
    FASEB J 24:740-9. 2010
  8. pmc Enhanced airway inflammation and remodeling in adenosine deaminase-deficient mice lacking the A2B adenosine receptor
    Yang Zhou
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Houston, TX 77030, USA
    J Immunol 182:8037-46. 2009
  9. doi request reprint Elevated ecto-5'-nucleotidase-mediated increased renal adenosine signaling via A2B adenosine receptor contributes to chronic hypertension
    Weiru Zhang
    Departments of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, TX 77030, USA
    Circ Res 112:1466-78. 2013
  10. pmc Excess adenosine in murine penile erectile tissues contributes to priapism via A2B adenosine receptor signaling
    Tiejuan Mi
    Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, Houston, Texas 77030, USA
    J Clin Invest 118:1491-501. 2008

Research Grants

  1. Adenosine Signaling and Lung Fibrosis
    Michael Blackburn; Fiscal Year: 2005
  2. Molecular Models of Adenosine Signaling and Asthma
    Michael Blackburn; Fiscal Year: 2007
  3. Adenosine Signaling and Lung Fibrosis
    Michael Blackburn; Fiscal Year: 2007
  4. ADENOSINE SIGNALING AND LUNG INFLAMMATION
    Michael Blackburn; Fiscal Year: 2001
  5. GENETIC MODEL FOR ASTHMA IN THE MOUSE
    Michael Blackburn; Fiscal Year: 2002
  6. Adenosine Signaling and Lung Fibrosis
    Michael R Blackburn; Fiscal Year: 2010

Collaborators

Detail Information

Publications76

  1. doi request reprint Hypoxia-induced deoxycytidine kinase expression contributes to apoptosis in chronic lung disease
    Tingting Weng
    1University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    FASEB J 27:2013-26. 2013
    ..Garcia-Morales, L. J., Molina, J. G., Pedroza, M., Bunge, R. R., Bruckner, B. A., Loebe, M., Seethamraju, H., and Blackburn, M. R. Hypoxia-induced deoxycytidine kinase expression contributes to apoptosis in chronic lung disease...
  2. pmc Metabolic consequences of adenosine deaminase deficiency in mice are associated with defects in alveogenesis, pulmonary inflammation, and airway obstruction
    M R Blackburn
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston Medical School, Houston, Texas 77030, USA
    J Exp Med 192:159-70. 2000
    ..These data suggest that disturbances in purinergic signaling mediate the lung inflammation and damage seen in ADA-deficient mice...
  3. ncbi request reprint A role for neural pathways in adenosine-induced bronchoconstriction
    Michael R Blackburn
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Am J Physiol Lung Cell Mol Physiol 293:L22-4. 2007
  4. ncbi request reprint The use of enzyme therapy to regulate the metabolic and phenotypic consequences of adenosine deaminase deficiency in mice. Differential impact on pulmonary and immunologic abnormalities
    M R Blackburn
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 275:32114-21. 2000
    ..This model will be useful in examining the efficacy of ADA enzyme therapy and studying the mechanisms underlying the immunodeficiency and pulmonary phenotypes associated with ADA deficiency...
  5. ncbi request reprint Too much of a good thing: adenosine overload in adenosine-deaminase-deficient mice
    Michael R Blackburn
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Houston, TX 77030, USA
    Trends Pharmacol Sci 24:66-70. 2003
    ....
  6. ncbi request reprint Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice
    Amir Mohsenin
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, University of Texas, Houston, Texas 77030, USA
    Am J Physiol Lung Cell Mol Physiol 293:L753-61. 2007
    ..There were no compensatory changes in the other adenosine receptors in the lungs of ADA/A(2A)R double knockout mice. These findings suggest that the A(2A)R plays a protective role in the ADA-deficient model of pulmonary inflammation...
  7. pmc Increased adenosine contributes to penile fibrosis, a dangerous feature of priapism, via A2B adenosine receptor signaling
    Jiaming Wen
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, 6431 Fannin St, MSB 6 200, Houston, TX 77030, USA
    FASEB J 24:740-9. 2010
    ..Dai, Y., Zhang, Y., Tang, Y., Sun, H., Mi, T., Phatarpekar, P. V., Kellems, R. E., Blackburn, M. R., Xia, Y. Increased adenosine contributes to penile fibrosis, a dangerous feature of priapism, via A(2B) adenosine receptor signaling...
  8. pmc Enhanced airway inflammation and remodeling in adenosine deaminase-deficient mice lacking the A2B adenosine receptor
    Yang Zhou
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Houston, TX 77030, USA
    J Immunol 182:8037-46. 2009
    ..These findings support an important protective role for A(2B)R signaling during acute stages of lung disease...
  9. doi request reprint Elevated ecto-5'-nucleotidase-mediated increased renal adenosine signaling via A2B adenosine receptor contributes to chronic hypertension
    Weiru Zhang
    Departments of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, TX 77030, USA
    Circ Res 112:1466-78. 2013
    ..Hypertension is the most prevalent life-threatening disease worldwide and is frequently associated with chronic kidney disease (CKD). However, the molecular basis underlying hypertensive CKD is not fully understood...
  10. pmc Excess adenosine in murine penile erectile tissues contributes to priapism via A2B adenosine receptor signaling
    Tiejuan Mi
    Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, Houston, Texas 77030, USA
    J Clin Invest 118:1491-501. 2008
    ..These findings provide insight regarding the molecular basis of priapism and suggest that strategies to either reduce adenosine or block A2BR activation may prove beneficial in the treatment of this disorder...
  11. pmc A3 adenosine receptor signaling influences pulmonary inflammation and fibrosis
    Eva Morschl
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Medical School, Houston, Texas77030, USA
    Am J Respir Cell Mol Biol 39:697-705. 2008
    ..Together, these results suggest that the A(3)R serves antiinflammatory functions in the bleomycin model, and is also involved in regulating the production of mediators that can impact fibrosis...
  12. ncbi request reprint Partially adenosine deaminase-deficient mice develop pulmonary fibrosis in association with adenosine elevations
    Janci L Chunn
    Dept of Biochemistry and Molecular Biology, Univ of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Am J Physiol Lung Cell Mol Physiol 290:L579-87. 2006
    ....
  13. pmc Role of A2B adenosine receptor signaling in adenosine-dependent pulmonary inflammation and injury
    Chun Xiao Sun
    Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston 77030, USA
    J Clin Invest 116:2173-2182. 2006
    ..Thus in chronic lung diseases associated with increased adenosine, antagonism of A2BAR-mediated responses may prove to be a beneficial therapy...
  14. pmc Distinct roles for the A2B adenosine receptor in acute and chronic stages of bleomycin-induced lung injury
    Yang Zhou
    Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, Houston, TX 77030, USA
    J Immunol 186:1097-106. 2011
    ..p. bleomycin. These studies highlight the distinct roles of A(2B)R signaling during acute and chronic stages of lung injury...
  15. pmc Alterations in adenosine metabolism and signaling in patients with chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis
    Yang Zhou
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Houston, Texas, United States of America
    PLoS ONE 5:e9224. 2010
    ..Changes include an up-regulation of CD73, the major enzyme of adenosine production and down-regulation of adenosine deaminase (ADA), the major enzyme for adenosine metabolism. In addition, adenosine receptors are elevated...
  16. doi request reprint Detrimental effects of adenosine signaling in sickle cell disease
    Yujin Zhang
    Department of Biochemistry and Molecular Biology, The University of Texas Health Science Center at Houston, Houston, Texas, USA
    Nat Med 17:79-86. 2011
    ..Thus, excessive adenosine signaling through the A(2B)R has a pathological role in SCD. These findings may provide new therapeutic possibilities for this disease...
  17. pmc Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease
    Daniel J Schneider
    Department of Biochemistry and Molecular Biology, The University of Texas Houston Medical School, 6431 Fannin Blvd, Houston, TX 77030, USA
    FASEB J 24:70-80. 2010
    ..These results suggest that OPN represents a novel biomarker and therapeutic target for patients with COPD...
  18. pmc Adenosine deaminase enzyme therapy prevents and reverses the heightened cavernosal relaxation in priapism
    Jiaming Wen
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Houston, TX 77030, USA
    J Sex Med 7:3011-22. 2010
    ..Recent studies have revealed a novel role of excess adenosine in priapism caused by heightened cavernosal relaxation, and therefore present an intriguing mechanism-based therapeutic possibility...
  19. pmc A2B adenosine receptor contributes to penile erection via PI3K/AKT signaling cascade-mediated eNOS activation
    Jiaming Wen
    Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, 6431 Fannin St, MSB 6 200, Houston, TX 77030, USA
    FASEB J 25:2823-30. 2011
    ..These studies suggest that this signaling pathway may be a novel therapeutic target for erectile disorders...
  20. ncbi request reprint A3 adenosine receptor signaling contributes to airway inflammation and mucus production in adenosine deaminase-deficient mice
    Hays W J Young
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Houston, TX 77030, USA
    J Immunol 173:1380-9. 2004
    ..These findings identify an important role for the A(3)R in regulating lung eosinophilia and mucus production in an environment of elevated adenosine...
  21. pmc A3 adenosine receptor signaling contributes to airway mucin secretion after allergen challenge
    Hays W J Young
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Medical School, 6431 Fannin, Houston, TX 77030, USA
    Am J Respir Cell Mol Biol 35:549-58. 2006
    ..Thus, the A(3)AR contributes to mucin secretion in allergen-induced metaplasia. Signaling through this receptor may contribute to mucus airway obstruction seen in pulmonary disorders in which adenosine levels are elevated...
  22. pmc Interleukin-6 contributes to inflammation and remodeling in a model of adenosine mediated lung injury
    Mesias Pedroza
    Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, Houston, Texas, United States of America
    PLoS ONE 6:e22667. 2011
    ..Based on these observations, we hypothesized that IL-6 signaling contributes to tissue destruction and remodeling in a model of chronic lung disease where adenosine levels are elevated...
  23. pmc A protective role for the A1 adenosine receptor in adenosine-dependent pulmonary injury
    Chun Xiao Sun
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston Medical School, Houston, Texas 77030, USA
    J Clin Invest 115:35-43. 2005
    ....
  24. ncbi request reprint Adenosine-dependent pulmonary fibrosis in adenosine deaminase-deficient mice
    Janci L Chunn
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Medical School, Houston, TX 77030, USA
    J Immunol 175:1937-46. 2005
    ..These findings demonstrate that chronic adenosine elevations are associated with pulmonary fibrosis in ADA-deficient mice and suggest that the adenosine functions as a profibrotic signal in the lung...
  25. ncbi request reprint Adenosine deaminase-deficient mice generated using a two-stage genetic engineering strategy exhibit a combined immunodeficiency
    M R Blackburn
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 273:5093-100. 1998
    ..ADA-deficient mice also exhibited severe pulmonary insufficiency, bone abnormalities, and kidney pathogenesis. These mice have provided in vivo information into the metabolic basis for the immune phenotype associated with ADA deficiency...
  26. pmc Osteopontin in systemic sclerosis and its role in dermal fibrosis
    Minghua Wu
    Division of Rheumatology and Clinical Immunogenetics, Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA
    J Invest Dermatol 132:1605-14. 2012
    ..Finally, TGF-β production by OPN-deficient macrophages was reduced compared with WT. These data demonstrate an important role for OPN in the development of dermal fibrosis and suggest that it may be a new therapeutic target in SSc...
  27. doi request reprint Interleukin 6 underlies angiotensin II-induced hypertension and chronic renal damage
    Weiru Zhang
    Department of Biochemistry, University of Texas Houston Medical School, Houston, TX, USA
    Hypertension 59:136-44. 2012
    ..These findings immediately suggest that the IL-6 signaling is a novel therapeutic target to manage this devastating disorder affecting millions worldwide...
  28. pmc Adenosine mediates IL-13-induced inflammation and remodeling in the lung and interacts in an IL-13-adenosine amplification pathway
    Michael R Blackburn
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Texas, USA
    J Clin Invest 112:332-44. 2003
    ..They also demonstrate that IL-13 and adenosine stimulate one another in an amplification pathway that may contribute to the nature, severity, progression, and/or chronicity of IL-13 and/or Th2-mediated disorders...
  29. ncbi request reprint Enhanced CXCL1 production and angiogenesis in adenosine-mediated lung disease
    Amir Mohsenin
    Department of Biochemistry and Molecular Biology, The University of Texas Houston Medical School, Houston, TX 77030, USA
    FASEB J 21:1026-36. 2007
    ..Our findings suggest that adenosine plays an important role, via CXCL1, in the induction of pulmonary angiogenesis...
  30. pmc The A2B adenosine receptor modulates pulmonary hypertension associated with interstitial lung disease
    Harry Karmouty-Quintana
    Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, 6431 Fannin, Houston, TX 77030, USA
    FASEB J 26:2546-57. 2012
    ..These studies identify a novel mechanism of disease progression to pulmonary hypertension and support the development of A(2B)R antagonists for the treatment of pulmonary hypertension secondary to interstitial lung disease...
  31. pmc Central role of Muc5ac expression in mucous metaplasia and its regulation by conserved 5' elements
    Hays W J Young
    Department of Pulmonary Medicine, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Am J Respir Cell Mol Biol 37:273-90. 2007
    ..In conclusion, Muc5ac expression is the central event in antigen-induced mucous metaplasia, and phylogenetically conserved 5' noncoding domains control its regulation...
  32. ncbi request reprint Impaired germinal center maturation in adenosine deaminase deficiency
    Melissa B Aldrich
    Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston, TX 77030, USA
    J Immunol 171:5562-70. 2003
    ..The altered splenic environment and signaling abnormalities may concurrently contribute to a block in B cell Ag-dependent maturation in ADA-deficient mouse spleens...
  33. ncbi request reprint Adenosine signaling in asthma and chronic obstructive pulmonary disease
    Amir Mohsenin
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Houston, Texas 77030, USA
    Curr Opin Pulm Med 12:54-9. 2006
    ..This review will summarize important findings regarding the role of adenosine, a signaling nucleoside implicated in the pathogenesis of these two disorders...
  34. ncbi request reprint Activation of murine lung mast cells by the adenosine A3 receptor
    Hongyan Zhong
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Houston, TX 77030, USA
    J Immunol 171:338-45. 2003
    ..These results demonstrate that the A(3) adenosine receptor plays an important role in adenosine-mediated murine lung mast cell degranulation...
  35. pmc Coordinate activation of inflammatory gene networks, alveolar destruction and neonatal death in AKNA deficient mice
    Wenbin Ma
    Department of Immunology, Unit 902, The University of Texas MD Anderson Cancer Center, 7455 Fannin Street, Houston, TX 77054, USA
    Cell Res 21:1564-77. 2011
    ....
  36. doi request reprint Adenosine signaling, priapism and novel therapies
    Yingbo Dai
    University of Texas at Houston Medical School, Biochemistry and Molecular Biology, Houston, TX 77030, USA
    J Sex Med 6:292-301. 2009
    ..Adenosine is a signaling nucleoside that elicits many pathophysiological effects by engaging membrane receptors. Recent evidence shows that adenosine may play an important role in priapism via adenosine receptor...
  37. pmc A role of erythrocytes in adenosine monophosphate initiation of hypometabolism in mammals
    Isadora Susan Daniels
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center, Houston, TX 77030, USA
    J Biol Chem 285:20716-23. 2010
    ..In summary, our investigations reveal that 5'-AMP mediated hypometabolism is probably triggered by reduced oxygen transport by erythrocytes initiated by uptake of 5'-AMP...
  38. pmc Coordinated changes in mRNA turnover, translation, and RNA processing bodies in bronchial epithelial cells following inflammatory stimulation
    Yuxin Zhai
    Department of Biochemistry and Molecular Biology, The University of Texas Houston Medical School, Houston, TX 77030, USA
    Mol Cell Biol 28:7414-26. 2008
    ..Our study reveals an orchestrated change among posttranscriptional mechanisms, which help sustain high levels of inflammatory mediator production in bronchial epithelium during the pathogenesis of inflammatory airway diseases...
  39. ncbi request reprint Abnormal alveolar development associated with elevated adenine nucleosides
    Suman K Banerjee
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Am J Respir Cell Mol Biol 30:38-50. 2004
    ..These studies provide evidence that elevated lung adenosine can lead to abnormal alveogenesis by disrupting patterns of cell proliferation and apoptosis...
  40. pmc Adenosine signaling during acute and chronic disease states
    Harry Karmouty-Quintana
    Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston, 6431 Fannin Blvd, Suite 6 200, Houston, TX 77030, USA
    J Mol Med (Berl) 91:173-81. 2013
    ....
  41. ncbi request reprint Coexpression of IL-5 and eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma
    Sergei I Ochkur
    Division of Pulmonary Medicine, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona, 13400 East Shea Boulevard, Scottsdale, AZ 85259, USA
    J Immunol 178:7879-89. 2007
    ..Genetic ablation of all eosinophils from this double transgenic model abolished the induced pulmonary pathologies, demonstrating that these pathologies are a consequence of one or more eosinophil effector functions...
  42. ncbi request reprint Ecto-5'-nucleotidase (CD73)-mediated adenosine production is tissue protective in a model of bleomycin-induced lung injury
    Jonathan B Volmer
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Medical School, Houston, 77030, USA
    J Immunol 176:4449-58. 2006
    ..Together, these findings suggest that CD73-dependent adenosine production contributes to anti-inflammatory pathways in bleomycin-induced lung injury...
  43. pmc Adenosine signaling and the regulation of chronic lung disease
    Yang Zhou
    Department of Biochemistry and Molecular Biology, The University of Texas Houston Medical School, 6431 Fannin St, Houston, Texas 77030, USA
    Pharmacol Ther 123:105-16. 2009
    ..This review discusses the current progress of research efforts and clinical trials aimed at understanding the complexities of these signaling pathway as they pertain to the development of treatment strategies for chronic lung diseases...
  44. ncbi request reprint Metabolic and immunologic consequences of limited adenosine deaminase expression in mice
    M R Blackburn
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 271:15203-10. 1996
    ..These mice represent the first genetic animal model for ADA deficiency and provide insight into the tissue-specific requirements of ADA...
  45. ncbi request reprint Adenosine-dependent airway inflammation and hyperresponsiveness in partially adenosine deaminase-deficient mice
    J L Chunn
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center, Houston Medical School, Houston, TX 77030, USA
    J Immunol 167:4676-85. 2001
    ..Collectively, these findings suggest that elevations in adenosine can directly impact lung inflammation and physiology...
  46. pmc Cadherin-11 contributes to pulmonary fibrosis: potential role in TGF-β production and epithelial to mesenchymal transition
    Daniel J Schneider
    Department of Biochemistry and Molecular Biology, Pediatric Research Center, University of Texas Health Science Center, Houston, Texas, USA
    FASEB J 26:503-12. 2012
    ..Together, these results identify CDH11 as a novel therapeutic target for pulmonary fibrosis...
  47. pmc A role for tumour necrosis factor-alpha, complement C5 and interleukin-6 in the initiation and development of the mycobacterial cord factor trehalose 6,6'-dimycolate induced granulomatous response
    Kerry J Welsh
    Medical School, University of Texas Houston, Houston, TX 77030, USA
    Microbiology 154:1813-24. 2008
    ..These data suggest that TNF-alpha is critical for initiation of the granulomatous response, C5a is necessary for formation of cohesive granulomas, and IL-6 plays a key role in the granuloma maintenance response to mycobacterial TDM...
  48. ncbi request reprint Adenosine deaminase deficiency: metabolic basis of immune deficiency and pulmonary inflammation
    Michael R Blackburn
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Houston, TX 77030 USA
    Adv Immunol 86:1-41. 2005
    ..Thus, the examination of ADA deficiency has identified the importance of purinergic signaling during lymphoid development and in the regulation of aspects of chronic lung disease...
  49. ncbi request reprint Genetically engineered mice demonstrate that adenosine deaminase is essential for early postimplantation development
    M R Blackburn
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030, USA
    Development 124:3089-97. 1997
    ..These experiments provide genetic evidence that Ada expression at the maternal-fetal interface is essential for early postimplantation development in mice...
  50. ncbi request reprint Adenosine-mediated mast cell degranulation in adenosine deaminase-deficient mice
    H Zhong
    Department of Biochemistry and Molecular Biology, The University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Pharmacol Exp Ther 298:433-40. 2001
    ....
  51. ncbi request reprint Certain surfactants show promise in the therapy of pulmonary tuberculosis
    James K Stoops
    UT Houston Medical School, 6431 Fannin St, Houston, TX 77030, USA
    In Vivo 24:687-94. 2010
    ..This protective barrier was removed by exposing the bacteria to certain surfactants...
  52. ncbi request reprint Gene expression profiling in inflammatory airway disease associated with elevated adenosine
    Suman K Banerjee
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Houston, Texas 77030, USA
    Am J Physiol Lung Cell Mol Physiol 282:L169-82. 2002
    ..These mice will serve as a useful in vivo model for characterizing molecular mechanisms of adenosine-mediated lung damage...
  53. ncbi request reprint Expression of capacitative calcium TrpC proteins in rat myometrium during pregnancy
    Lidiya G Babich
    Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, Houston, Texas 77030, USA
    Biol Reprod 70:919-24. 2004
    ..These data suggest that rTrpC4 may play a major role in regulated Ca2+ entry in myometrial cells and throughout pregnancy but do not rule out contributions from other Trp proteins...
  54. pmc Disruption of the adenosine deaminase gene causes hepatocellular impairment and perinatal lethality in mice
    M Wakamiya
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 92:3673-7. 1995
    ..The results presented here demonstrate that ADA is important for the homeostatic maintenance of purines in mice...
  55. ncbi request reprint Tissue-specific rescue suggests that placental adenosine deaminase is important for fetal development in mice
    M R Blackburn
    Verna and Mars McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 270:23891-4. 1995
    ..Our findings suggest that placental ADA is important for murine fetal development and illustrate a general strategy for the tissue specific correction of phenotypes associated with null mutations in mice...
  56. pmc Endogenous osteopontin promotes ozone-induced neutrophil recruitment to the lungs and airway hyperresponsiveness to methacholine
    Ramon X Barreno
    Division of Pediatric Critical Care Medicine, Department of Pediatrics, The University of Texas Medical School at Houston, Houston, TX 77030, USA
    Am J Physiol Lung Cell Mol Physiol 305:L118-29. 2013
    ....
  57. pmc Adenosine and dopamine receptors coregulate photoreceptor coupling via gap junction phosphorylation in mouse retina
    Hongyan Li
    Richard S Ruiz, MD, Department of Ophthalmology and Visual Science, The University of Texas Medical School, Houston, Texas 77030, USA
    J Neurosci 33:3135-50. 2013
    ..We conclude that adenosine and dopamine coregulate photoreceptor coupling through opposite action on the PKA pathway and Cx36 phosphorylation. In addition, loss of the A2aR hampered D4R gene expression and function...
  58. ncbi request reprint Diverse genetic regulatory motifs required for murine adenosine deaminase gene expression in the placenta
    D Shi
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 272:2334-41. 1997
    ..These findings indicate that multiple protein binding motifs are necessary for Ada expression in the placenta...
  59. ncbi request reprint Mucin is produced by clara cells in the proximal airways of antigen-challenged mice
    Christopher M Evans
    Pulmonary Medicine, M D Anderson Cancer Center, 2121 West Holcombe Blvd, Houston, TX 77030, USA
    Am J Respir Cell Mol Biol 31:382-94. 2004
    ..Thus, Clara cells show great plasticity in structure and secretory products, yet have molecular and functional continuity in their identity as specialized apical secretory cells...
  60. ncbi request reprint Constant darkness is a circadian metabolic signal in mammals
    Jianfa Zhang
    Department of Biochemistry and Molecular Biology, University of Texas Health Science Center, Houston, Texas 77030, USA
    Nature 439:340-3. 2006
    ..Induction of mClps expression by 5'-AMP in LD mice was reciprocally linked to blood glucose levels. Our findings uncover a circadian metabolic rhythm in mammals...
  61. pmc Adenosine receptors in regulation of dendritic cell differentiation and function
    Sergey V Novitskiy
    Division of Hematology Oncology, Vanderbilt University, Nashville, TN 37232, USA
    Blood 112:1822-31. 2008
    ..Adenosine signaling through A(2B) receptor is an important factor of aberrant DC differentiation and generation of tolerogenic, angiogenic, and proinflammatory cells...
  62. ncbi request reprint Mechanisms of apoptosis in developing thymocytes as revealed by adenosine deaminase-deficient fetal thymic organ cultures
    Linda F Thompson
    Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, 825 NE, 13th Street, Oklahoma City, OK 73104, USA
    Biochem Pharmacol 66:1595-9. 2003
    ..These data suggest that thymocyte apoptosis as a consequence of failing developmental checkpoints involves one or more caspases that are not regulated by Bcl-2...
  63. ncbi request reprint In vivo transduction by intravenous injection of a lentiviral vector expressing human ADA into neonatal ADA gene knockout mice: a novel form of enzyme replacement therapy for ADA deficiency
    Denise A Carbonaro
    Division of Research Immunology B M T, Childrens Hospital Los Angeles, California 90027, USA
    Mol Ther 13:1110-20. 2006
    ..These studies illustrate the potential for sustained in vivo production of enzymatically active ADA, as an alternative to therapy by frequent injection of exogenous ADA protein...
  64. pmc Adenosine kinase inhibition promotes survival of fetal adenosine deaminase-deficient thymocytes by blocking dATP accumulation
    C Justin Van De Wiele
    Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
    J Clin Invest 110:395-402. 2002
    ..Our data are consistent with dATP-induced mitochondrial cytochrome c release followed by apoptosis as the mechanism by which ADA deficiency leads to reduced thymic T cell production...
  65. ncbi request reprint Effects of adenosine deaminase and A1 receptor deficiency in normoxic and ischaemic mouse hearts
    Laura Willems
    Heart Foundation Research Center, Griffith University, Southport, QLD 4217, Australia
    Cardiovasc Res 71:79-87. 2006
    ..Herein we assess effects of ADA (and A1AR) deficiency on AR-mediated responses and ischaemic tolerance...
  66. pmc Further differentiation of murine double-positive thymocytes is inhibited in adenosine deaminase-deficient murine fetal thymic organ culture
    C Justin Van De Wiele
    Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, 825 Northeast 13th Street, Oklahoma City, OK 73104, USA
    J Immunol 176:5925-33. 2006
    ..These data suggest that ADA deficiency leads to the induction of mitochondria-dependent apoptosis as a consequence of the accumulation of dATP derived from thymocytes failing the positive/negative selection checkpoint...
  67. pmc Effect of A2B adenosine receptor gene ablation on proinflammatory adenosine signaling in mast cells
    Sergey Ryzhov
    Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University, Nashville, TN 37232, USA
    J Immunol 180:7212-20. 2008
    ..Thus, our study confirmed the proinflammatory role of adenosine signaling via A(2B) receptors and the anti-inflammatory actions of A(2B) antagonists in mouse BMMCs...
  68. pmc Pharmacological blockade of A2A receptors prevents dermal fibrosis in a model of elevated tissue adenosine
    Patricia Fernandez
    Department of Medicine, New York University School of Medicine, NewYork, New York 10016, USA
    Am J Pathol 172:1675-82. 2008
    ..These data confirm a fibrogenic role for adenosine in the skin and reveal A(2A) receptor antagonists as novel therapeutic agents for the modulation of dermal fibrotic disorders...
  69. pmc CD73-generated adenosine restricts lymphocyte migration into draining lymph nodes
    Masahide Takedachi
    Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, 825 Northeast 13th Street, Oklahoma City, OK 73104, USA
    J Immunol 180:6288-96. 2008
    ..Adenosine receptor signaling to restrict lymphocyte migration across HEV may be an important mechanism to control the magnitude of an inflammatory response...
  70. pmc Neonatal bone marrow transplantation of ADA-deficient SCID mice results in immunologic reconstitution despite low levels of engraftment and an absence of selective donor T lymphoid expansion
    Denise A Carbonaro
    Division of Research Immunology Bone Marrow Transplantation, Childrens Hospital, Los Angeles, CA 90027, USA
    Blood 111:5745-54. 2008
    ..Thus, ADA-deficient SCID is unique in its responses to nonmyeloablative bone marrow transplantation, which has implications for clinical bone marrow transplantation or gene therapy...
  71. ncbi request reprint Effect of A2B adenosine receptor gene ablation on adenosine-dependent regulation of proinflammatory cytokines
    Sergey Ryzhov
    Divisions of Cardiovascular Medicine, Vanderbilt University, Nashville, Tennessee, USA
    J Pharmacol Exp Ther 324:694-700. 2008
    ....
  72. pmc A2A adenosine receptors and C/EBPbeta are crucially required for IL-10 production by macrophages exposed to Escherichia coli
    Balázs Csóka
    Department of Surgery, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07103, USA
    Blood 110:2685-95. 2007
    ..C/EBPbeta-deficient macrophages failed to produce IL-10 in response to adenosine and E coli. Our results suggest that the A(2A) receptor-C/EBPbeta axis is critical for IL-10 production after bacterial infection...
  73. ncbi request reprint Genetic modulation of adenosine receptor function and adenosine handling in murine hearts: insights and issues
    Kevin J Ashton
    Heart Foundation Research Centre, Griffith University Southport, QLD 4217, Australia
    J Mol Cell Cardiol 42:693-705. 2007
    ..However, some outcomes are both novel and controversial. This review outlines AR-modified murine models currently under study from the perspective of cardiovascular phenotype...
  74. ncbi request reprint Modulation of ischaemic contracture in mouse hearts: a 'supraphysiological' response to adenosine
    Melissa E Reichelt
    Heart Foundation Research Centre, Griffith University, Southport, QLD 4217, Australia
    Exp Physiol 92:175-85. 2007
    ....
  75. pmc Adenosine metabolism and murine strain-specific IL-4-induced inflammation, emphysema, and fibrosis
    Bing Ma
    Section of Pulmonary and Critical Care Medicine, Yale University School of Medicine, New Haven, Connecticut 06519, USA
    J Clin Invest 116:1274-83. 2006
    ..They also demonstrate that IL-4 has different effects on adenosine metabolism in Balb/c and C57BL/6 mice and that these differences contribute to the different responses that IL-4 induces in these inbred animals...
  76. pmc Inhibition of deoxynucleoside kinases in human thymocytes prevents dATP accumulation and induction of apoptosis
    Michelle L Joachims
    Department of Immunobiology and Cancer, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
    Nucleosides Nucleotides Nucleic Acids 27:816-20. 2008
    ..Thus, both deoxynucleoside kinases are involved in this model of ADA deficiency...

Research Grants23

  1. Adenosine Signaling and Lung Fibrosis
    Michael Blackburn; Fiscal Year: 2005
    ..abstract_text> ..
  2. Molecular Models of Adenosine Signaling and Asthma
    Michael Blackburn; Fiscal Year: 2007
    ..These studies will lead to a better understanding of adenosine's impact on the severity of chronic lung diseases such as asthma and COPD, which will help guide the development of novel therapies for their treatment. ..
  3. Adenosine Signaling and Lung Fibrosis
    Michael Blackburn; Fiscal Year: 2007
    ..These experiments will help guide the development of novel therapeutic approaches to treat pulmonary disorders in which fibrosis is a component. ..
  4. ADENOSINE SIGNALING AND LUNG INFLAMMATION
    Michael Blackburn; Fiscal Year: 2001
    ..4. Evaluate acute and long-term changes of airway function in ADA deficient mice as well as ADA deficient mice exposed to environmental challenges. ..
  5. GENETIC MODEL FOR ASTHMA IN THE MOUSE
    Michael Blackburn; Fiscal Year: 2002
    ..4. Identify and characterize the adenosine receptor subtypes involved in the inflammation and airway damage seen in ADA deficient mice. ..
  6. Adenosine Signaling and Lung Fibrosis
    Michael R Blackburn; Fiscal Year: 2010
    ..These experiments will help guide the development of novel therapeutic approaches to treat pulmonary disorders in which fibrosis is a component. ..