Affiliation: Temple University
Madesh M, Zong W, Hawkins B, Ramasamy S, Venkatachalam T, Mukhopadhyay P, et al
. Execution of superoxide-induced cell death by the proapoptotic Bcl-2-related proteins Bid and Bak. Mol Cell Biol. 2009;29:3099-112 pubmed publisher
Shanmughapriya S, Rajan S, Hoffman N, Zhang X, Guo S, Kolesar J, et al
. Ca2+ signals regulate mitochondrial metabolism by stimulating CREB-mediated expression of the mitochondrial Ca2+ uniporter gene MCU. Sci Signal. 2015;8:ra23 pubmed publisher
..Thus, our results showed that a transcriptional regulatory circuit involving Ca2+ -dependent activation of CREB controls the Ca2+ uptake capability of mitochondria and hence regulates mitochondrial metabolism. ..
Nemani N, Carvalho E, Tomar D, Dong Z, Ketschek A, Breves S, et al
. MIRO-1 Determines Mitochondrial Shape Transition upon GPCR Activation and Ca2+ Stress. Cell Rep. 2018;23:1005-1019 pubmed publisher
..Functionally, Miro1-dependent MiST is essential for autophagy/mitophagy that is attenuated in Miro1 EF1 mutants. Thus, Miro1 is a cytosolic Ca2+ sensor that decodes metazoan Ca2+ signals as MiST. ..
Nemani N, Shanmughapriya S, Madesh M. Molecular regulation of MCU: Implications in physiology and disease. Cell Calcium. 2018;74:86-93 pubmed publisher
..The mode of regulation either inhibits or enhances MCU channel activity and thus regulates mitochondrial metabolism and cell fate. ..
Alevriadou B, Shanmughapriya S, Patel A, Stathopulos P, Madesh M. Mitochondrial Ca2+ transport in the endothelium: regulation by ions, redox signalling and mechanical forces. J R Soc Interface. 2017;14: pubmed publisher
..Thus, we also propose an EC mechanotransduction-mediated regulation of MCU activity in the context of vascular physiology and atherosclerotic vascular disease. ..
Doonan P, Chandramoorthy H, Hoffman N, Zhang X, Cardenas C, Shanmughapriya S, et al
. LETM1-dependent mitochondrial Ca2+ flux modulates cellular bioenergetics and proliferation. FASEB J. 2014;28:4936-49 pubmed publisher
..Reconstitution of LETM1 or antioxidant overexpression rescued mitochondrial Ca(2+) transport and bioenergetics. These findings reveal the role of LETM1-dependent mitochondrial Ca(2+) flux in shaping cellular bioenergetics. ..
Shanmughapriya S, Rajan S, Hoffman N, Higgins A, Tomar D, Nemani N, et al
. SPG7 Is an Essential and Conserved Component of the Mitochondrial Permeability Transition Pore. Mol Cell. 2015;60:47-62 pubmed publisher
..This study identifies an ubiquitously expressed IMM integral protein, SPG7, as a core component of the PTP at the OMM and IMM contact site. ..
Tomar D, Dong Z, Shanmughapriya S, Koch D, Thomas T, Hoffman N, et al
. MCUR1 Is a Scaffold Factor for the MCU Complex Function and Promotes Mitochondrial Bioenergetics. Cell Rep. 2016;15:1673-85 pubmed publisher
..These studies establish the existence of a MCU complex that assembles at the mitochondrial integral membrane and regulates Ca(2+)-dependent mitochondrial metabolism. ..
Dong Z, Shanmughapriya S, Tomar D, Siddiqui N, Lynch S, Nemani N, et al
. Mitochondrial Ca2+ Uniporter Is a Mitochondrial Luminal Redox Sensor that Augments MCU Channel Activity. Mol Cell. 2017;65:1014-1028.e7 pubmed publisher
..In contrast, these effects were largely independent of MCU interaction with its regulators. These findings reveal a distinct functional role for Cys-97 in ROS sensing and regulation of MCU activity. ..