Eugen Brailoiu

Summary

Affiliation: Temple University
Country: USA

Publications

  1. pmc Urocortin 3 elevates cytosolic calcium in nucleus ambiguus neurons
    G Cristina Brailoiu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    J Neurochem 122:1129-36. 2012
  2. pmc State-dependent calcium mobilization by urotensin-II in cultured human endothelial cells
    Eugen Brailoiu
    Department of Pharmacology, 3420 N Broad Street, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Peptides 29:721-6. 2008
  3. ncbi request reprint Distribution and characterization of estrogen receptor G protein-coupled receptor 30 in the rat central nervous system
    Eugen Brailoiu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    J Endocrinol 193:311-21. 2007
  4. pmc Mechanisms of G protein-coupled estrogen receptor-mediated spinal nociception
    Elena Deliu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Pain 13:742-54. 2012
  5. pmc Expression of estrogen receptor GPR30 in the rat spinal cord and in autonomic and sensory ganglia
    Siok L Dun
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Neurosci Res 87:1610-9. 2009
  6. pmc Central role of Gq in the hypertrophic signal transduction of angiotensin II in vascular smooth muscle cells
    Haruhiko Ohtsu
    Cardiovascular Research Center and Department of Physiology, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, PA 19140, USA
    Endocrinology 149:3569-75. 2008
  7. pmc Intracellular angiotensin II activates rat myometrium
    Elena Deliu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennslyvania 19140, USA
    Am J Physiol Cell Physiol 301:C559-65. 2011
  8. pmc G protein-coupled estrogen receptor 1-mediated effects in the rat myometrium
    Andrei A Tica
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Am J Physiol Cell Physiol 301:C1262-9. 2011
  9. pmc Endothelial nitric oxide synthase inhibits G12/13 and rho-kinase activated by the angiotensin II type-1 receptor: implication in vascular migration
    Hiroyuki Suzuki
    Cardiovascular Research Center, Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Arterioscler Thromb Vasc Biol 29:217-24. 2009
  10. pmc Intracellular endothelin type B receptor-driven Ca2+ signal elicits nitric oxide production in endothelial cells
    Elena Deliu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 287:41023-31. 2012

Collaborators

Detail Information

Publications43

  1. pmc Urocortin 3 elevates cytosolic calcium in nucleus ambiguus neurons
    G Cristina Brailoiu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    J Neurochem 122:1129-36. 2012
    ....
  2. pmc State-dependent calcium mobilization by urotensin-II in cultured human endothelial cells
    Eugen Brailoiu
    Department of Pharmacology, 3420 N Broad Street, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Peptides 29:721-6. 2008
    ..The result demonstrates a state-dependent effect of U-II in cultured HAEC, which may explain the variable responses to U-II under different experimental conditions...
  3. ncbi request reprint Distribution and characterization of estrogen receptor G protein-coupled receptor 30 in the rat central nervous system
    Eugen Brailoiu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    J Endocrinol 193:311-21. 2007
    ....
  4. pmc Mechanisms of G protein-coupled estrogen receptor-mediated spinal nociception
    Elena Deliu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Pain 13:742-54. 2012
    ..These findings extend the current knowledge on GPER involvement in physiology and disease, providing the first evidence of its pronociceptive effects at central levels and characterizing some of the underlying mechanisms...
  5. pmc Expression of estrogen receptor GPR30 in the rat spinal cord and in autonomic and sensory ganglia
    Siok L Dun
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Neurosci Res 87:1610-9. 2009
    ....
  6. pmc Central role of Gq in the hypertrophic signal transduction of angiotensin II in vascular smooth muscle cells
    Haruhiko Ohtsu
    Cardiovascular Research Center and Department of Physiology, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, PA 19140, USA
    Endocrinology 149:3569-75. 2008
    ..Vascular G(q) seems to be a critical target of intervention against cardiovascular diseases associated with the enhanced renin-angiotensin system...
  7. pmc Intracellular angiotensin II activates rat myometrium
    Elena Deliu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennslyvania 19140, USA
    Am J Physiol Cell Physiol 301:C559-65. 2011
    ....
  8. pmc G protein-coupled estrogen receptor 1-mediated effects in the rat myometrium
    Andrei A Tica
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Am J Physiol Cell Physiol 301:C1262-9. 2011
    ..Taken together, our results indicate that GPER is expressed and functional in rat myometrium. GPER activation produces depolarization, elevates [Ca(2+)](i) and increases contractility in myometrial cells...
  9. pmc Endothelial nitric oxide synthase inhibits G12/13 and rho-kinase activated by the angiotensin II type-1 receptor: implication in vascular migration
    Hiroyuki Suzuki
    Cardiovascular Research Center, Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Arterioscler Thromb Vasc Biol 29:217-24. 2009
    ..Although, endothelial nitric oxide (NO) synthase (eNOS) is believed to antagonize vascular remodeling induced by the angiotensin II (AngII) type-1 receptor, the exact signaling mechanism remains unclear...
  10. pmc Intracellular endothelin type B receptor-driven Ca2+ signal elicits nitric oxide production in endothelial cells
    Elena Deliu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 287:41023-31. 2012
    ..Endothelin-1 acts in an intracrine fashion on endolysosomal ET(B) to induce nitric oxide formation, thus modulating endothelial function...
  11. pmc Agonist-selective effects of opioid receptor ligands on cytosolic calcium concentration in rat striatal neurons
    G Cristina Brailoiu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Drug Alcohol Depend 123:277-81. 2012
    ..Buprenorphine is an opioid receptor ligand whose mechanism of action is incompletely understood...
  12. ncbi request reprint Nesfatin-1: distribution and interaction with a G protein-coupled receptor in the rat brain
    G Cristina Brailoiu
    Department of Pharmacology, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, PA 19140, USA
    Endocrinology 148:5088-94. 2007
    ....
  13. ncbi request reprint Insulin-like peptide 5: expression in the mouse brain and mobilization of calcium
    Siok L Dun
    Department of Pharmacology, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, Pennsylvania 19140, USA
    Endocrinology 147:3243-8. 2006
    ..The concentration of irINSL5 in the hypothalamic-pituitary axis suggests a neuroendocrine function of this insulin superfamily member...
  14. pmc An NAADP-gated two-pore channel targeted to the plasma membrane uncouples triggering from amplifying Ca2+ signals
    Eugen Brailoiu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 285:38511-6. 2010
    ..We therefore uncouple TPC2 from amplification pathways and prove that it is a pore-forming subunit of an NAADP-gated Ca(2+) channel...
  15. ncbi request reprint KiSS-1 expression and metastin-like immunoreactivity in the rat brain
    G Cristina Brailoiu
    Department of Pharmacology, East Tennessee State University, Johnson City, Tennessee 37614, USA
    J Comp Neurol 481:314-29. 2005
    ..The results show that metastin is biologically active in rat central neurons, and its anatomical distribution suggests a possible role in nociception and autonomic and neuroendocrine functions...
  16. pmc Modulation of spontaneous transmitter release from the frog neuromuscular junction by interacting intracellular Ca(2+) stores: critical role for nicotinic acid-adenine dinucleotide phosphate (NAADP)
    Eugen Brailoiu
    Department of Pharmacology, James H Quillen College of Medicine, East Tennessee State University, PO Box 70577, Johnson City, TN 37614, USA
    Biochem J 373:313-8. 2003
    ..Asynchronous neurotransmitter release may therefore involve functional coupling of endoplasmic reticulum Ca(2+) stores with distinct Ca(2+) stores targeted by NAADP...
  17. pmc Acidic NAADP-sensitive calcium stores in the endothelium: agonist-specific recruitment and role in regulating blood pressure
    G Cristina Brailoiu
    Departments of Pharmacology, University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 285:37133-7. 2010
    ..Our data extend the actions of NAADP to the endothelium both in vitro and in vivo, pointing to a previously unrecognized role for this messenger in controlling blood pressure...
  18. ncbi request reprint Extra- and intracellular sphingosylphosphorylcholine promote spontaneous transmitter release from frog motor nerve endings
    Eugen Brailoiu
    Department of Pharmacology, James H Quillen College of Medicine, East Tennessee State University, PO Box 70577, Johnson City, TN 37614 1708, USA
    Mol Pharmacol 63:1430-6. 2003
    ..The result provides the first electrophysiological evidence that SPC can modulate transmitter release by an extra- or intracellular action at the frog motor nerve ending...
  19. ncbi request reprint Smooth muscle-associated protein 8: distribution and biological activity in the rat brain
    G Cristina Brailoiu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Neurosci Res 85:1789-96. 2007
    ..This is the first evidence of irSMAP8 in a population of VP/OT-containing hypothalamic neurons in the rat, and the peptide is biologically active in hypothalamic neurons, as evidenced by mobilization of extracellular Ca2+...
  20. ncbi request reprint Nicotinic acid adenine dinucleotide phosphate potentiates neurite outgrowth
    Eugen Brailoiu
    Department of Pharmacology, Temple University Medical School, Philadelphia, PA 19140, USA
    J Biol Chem 280:5646-50. 2005
    ..Interplay between NAADP receptors and more established intracellular Ca(2+) channels may therefore play important signaling roles in the nervous system...
  21. ncbi request reprint Expression and activity of cocaine- and amphetamine-regulated transcript peptide(1-39) in the rat
    Siok L Dun
    Department of Pharmacology, Temple University School of Medicine, 3400 N Broad Street, Philadelphia, PA 19140, USA
    Regul Pept 140:47-54. 2007
    ....
  22. ncbi request reprint Distribution and biological activity of obestatin in the rat
    Siok L Dun
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Endocrinol 191:481-9. 2006
    ....
  23. ncbi request reprint Inositol derivatives modulate spontaneous transmitter release at the frog neuromuscular junction
    Eugen Brailoiu
    East Tennessee State University, Department of Pharmacology, James H Quillen College of Medicine, P O Box 70577, Johnson City, TN 37614, USA
    Neuropharmacology 45:691-701. 2003
    ....
  24. pmc Photoaffinity labeling of nicotinic acid adenine dinucleotide phosphate (NAADP) targets in mammalian cells
    Yaping Lin-Moshier
    Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Biol Chem 287:2296-307. 2012
    ..This observation necessitates critical evaluation of current models of NAADP-triggered activation of the TPC family...
  25. doi request reprint NAADP-mediated channel 'chatter' in neurons of the rat medulla oblongata
    G Cristina Brailoiu
    Department of Pharmacology, Temple University School of Medicine, 3420 N Broad St, Philadelphia, PA 19140, USA
    Biochem J 419:91-7, 2 p following 97. 2009
    ..Thus NAADP is capable of co-ordinating channels both within the cell interior and at the cell membrane representing a novel mechanism for excitation of central neurons...
  26. pmc The G protein-coupled receptor GPR30 inhibits proliferation of estrogen receptor-positive breast cancer cells
    Eric A Ariazi
    Fox Chase Cancer Center Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania, USA
    Cancer Res 70:1184-94. 2010
    ..Further, flow cytometry showed that G-1 blocked MCF-7 cell cycle progression at the G(1) phase. Thus, GPR30 antagonizes growth of ERalpha-positive breast cancer and may represent a new target to combat this disease...
  27. pmc An ancestral deuterostome family of two-pore channels mediates nicotinic acid adenine dinucleotide phosphate-dependent calcium release from acidic organelles
    Eugen Brailoiu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 285:2897-901. 2010
    ..Our data highlight the functional divergence of this novel gene family during deuterostome evolution and provide further evidence that NAADP mediates calcium release from acidic stores through activation of TPCs...
  28. ncbi request reprint Messenger-specific role for nicotinic acid adenine dinucleotide phosphate in neuronal differentiation
    Eugen Brailoiu
    Department of Pharmacology, Temple University Medical School, Philadelphia, Pennsylvania, USA
    J Biol Chem 281:15923-8. 2006
    ..Thus, differential recruitment of intracellular Ca2+-mobilizing messengers and their target Ca2+ stores may represent a robust means of maintaining stimulus fidelity in the control of Ca2+-dependent cell function...
  29. pmc Essential requirement for two-pore channel 1 in NAADP-mediated calcium signaling
    Eugen Brailoiu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    J Cell Biol 186:201-9. 2009
    ..Thus, TPC1 is critical for NAADP action and is likely the long sought after target channel for NAADP...
  30. ncbi request reprint Acute exposure to ethanol potentiates human immunodeficiency virus type 1 Tat-induced Ca(2+) overload and neuronal death in cultured rat cortical neurons
    Eugen Brailoiu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Neurovirol 12:17-24. 2006
    ..The result led the authors to conclude that alcohol exposure significantly potentiates Tat-induced calcium overload and neuronal death...
  31. doi request reprint Surface-enhanced Raman spectroscopy as a tool for detecting Ca2+ mobilizing second messengers in cell extracts
    Elina A Vitol
    Department of Electrical and Computer Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USA
    Anal Chem 82:6770-4. 2010
    ..With a SERS sensor, NAADP can be detected in less than 1 min without any special sample preparation. To the best of our knowledge, this is the first demonstration of using SERS for calcium signaling applications...
  32. ncbi request reprint Differential regulation of homocysteine transport in vascular endothelial and smooth muscle cells
    Xiaohua Jiang
    Temple University School of Medicine, Department of Pharmacology, 3420 North Broad Street, Philadelphia, PA 19140, USA
    Arterioscler Thromb Vasc Biol 27:1976-83. 2007
    ..This study characterized and directly compared Hcy transport in cultured human aortic ECs (HAECs) and smooth muscle cells (HASMCs)...
  33. pmc Intracellular cannabinoid type 1 (CB1) receptors are activated by anandamide
    G Cristina Brailoiu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 286:29166-74. 2011
    ..In addition, our results support a novel role for nicotinic acid-adenine dinucleotide phosphate in cannabinoid-induced calcium signaling...
  34. ncbi request reprint ADAM17 mediates epidermal growth factor receptor transactivation and vascular smooth muscle cell hypertrophy induced by angiotensin II
    Haruhiko Ohtsu
    Cardiovascular Research Center, Temple University School of Medicine, 3420 N Broad St, Philadelphia, PA 19140, USA
    Arterioscler Thromb Vasc Biol 26:e133-7. 2006
    ..However, the identity of the metalloprotease responsible for this process remains unknown...
  35. doi request reprint Nesfatin-1 activates cardiac vagal neurons of nucleus ambiguus and elicits bradycardia in conscious rats
    G Cristina Brailoiu
    Department of Pharmaceutical Sciences, Thomas Jefferson University, Jefferson School of Pharmacy, Philadelphia, Pennsylvania, USA
    J Neurochem 126:739-48. 2013
    ..In vivo studies indicate that microinjection of nesfatin-1 into nucleus ambiguus produces bradycardia in conscious rats. This is the first report that nesfatin-1 increases the parasympathetic cardiac tone. ..
  36. ncbi request reprint Carbon nanopipettes characterize calcium release pathways in breast cancer cells
    Michael G Schrlau
    Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Nanotechnology 19:325102. 2008
    ..Their advantages over commonly used glass pipettes include smaller size, breakage and clogging resistance, and potential for multifunctionality such as in concurrent injection and electrical measurements...
  37. ncbi request reprint Urotensin-II regulates intracellular calcium in dissociated rat spinal cord neurons
    Catalin M Filipeanu
    Department of Pharmacology, James H Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614, USA
    J Neurochem 83:879-84. 2002
    ..Present results demonstrate that U-II modulates [Ca2+]i in rat spinal cord neurons via protein kinase A cascade...
  38. pmc Calmodulin increases transmitter release by mobilizing quanta at the frog motor nerve terminal
    Eugen Brailoiu
    Department of Pharmacology, James H Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee, TN 37614 1708, USA
    Br J Pharmacol 137:719-27. 2002
    ..The decreases in MEPP size and m produced by W7 were both reversed by addition of CaM. Our results suggest that CaM increases transmitter release by mobilizing synaptic vesicles at the frog motor nerve terminal...
  39. doi request reprint Human type II pneumocyte chemotactic responses to CXCR3 activation are mediated by splice variant A
    Rong Ji
    Division of Pulmonary Disease and Critical Care Medicine, Department of Medicine, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    Am J Physiol Lung Cell Mol Physiol 294:L1187-96. 2008
    ..Expression of CXCR3 in alveolar epithelial cells may be important in pneumocyte repair from injury...
  40. ncbi request reprint Ultra low concentrations of morphine increase neurite outgrowth in cultured rat spinal cord and cerebral cortical neurons
    Eugen Brailoiu
    Department of Pharmacology, James H Quillen College of Medicine, East Tennessee State University, PO Box 70577, Johnson City, TN 37614, USA
    Neurosci Lett 365:10-3. 2004
    ..Pretreatment with naloxone did not prevent the morphine effect. The result shows that morphine at ultra low concentrations enhances neurite outgrowth of spinal and cortical neurons via a naloxone-independent mechanism...
  41. ncbi request reprint G protein coupling and second messenger generation are indispensable for metalloprotease-dependent, heparin-binding epidermal growth factor shedding through angiotensin II type-1 receptor
    Mizuo Mifune
    Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 280:26592-9. 2005
    ....
  42. pmc Deregulation of microRNAs by HIV-1 Vpr protein leads to the development of neurocognitive disorders
    Ruma Mukerjee
    Department of Neurology, Molecular Studies of Neurodegenerative Diseases Laboratory, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 286:34976-85. 2011
    ..Therefore, we conclude that Vpr plays a major role in neuronal dysfunction through deregulating microRNAs and their target genes, a phenomenon that could lead to the development of neurocognitive disorders...
  43. pmc Sphingosine 1-phosphate enhances spontaneous transmitter release at the frog neuromuscular junction
    Eugen Brailoiu
    Department of Pharmacology, James H Quillen College of Medicine, East Tennessee State University, PO Box 70577, Johnson City 37614, USA
    Br J Pharmacol 136:1093-7. 2002
    ..The result provides the first evidence that S1-P acting intracellularly but not extracellularly enhances spontaneous transmitter release at the frog neuromuscular junction...

Research Grants3

  1. Role of nonnuclear estrogen receptor GPR30 in preganglionic vagal neurons
    Eugen Brailoiu; Fiscal Year: 2009
    ..A better understanding of the mechanisms of action of estrogen is critical for the development of new treatments for cardiovascular disease. ..
  2. Role of nonnuclear estrogen receptor GPR30 in preganglionic vagal neurons
    Eugen Brailoiu; Fiscal Year: 2010
    ..A better understanding of the mechanisms of action of estrogen is critical for the development of new treatments for cardiovascular disease. ..
  3. Role of nonnuclear estrogen receptor GPR30 in preganglionic vagal neurons
    Eugen Brailoiu; Fiscal Year: 2009
    ..A better understanding of the mechanisms of action of estrogen is critical for the development of new treatments for cardiovascular disease. ..