Research Topics
Genomes and Genes
| Eugen BrailoiuSummaryAffiliation: Temple University Country: USA Publications
Research Grants
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Detail Information
Publications
Urocortin 3 elevates cytosolic calcium in nucleus ambiguus neuronsG Cristina Brailoiu
Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
J Neurochem 122:1129-36. 2012....
Distribution and characterization of estrogen receptor G protein-coupled receptor 30 in the rat central nervous systemEugen Brailoiu
Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
J Endocrinol 193:311-21. 2007....- State-dependent calcium mobilization by urotensin-II in cultured human endothelial cellsEugen Brailoiu
Department of Pharmacology, 3420 N Broad Street, Temple University School of Medicine, Philadelphia, PA 19140, USA
Peptides 29:721-6. 2008..The result demonstrates a state-dependent effect of U-II in cultured HAEC, which may explain the variable responses to U-II under different experimental conditions... - Mechanisms of G protein-coupled estrogen receptor-mediated spinal nociceptionElena Deliu
Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Pain 13:742-54. 2012..These findings extend the current knowledge on GPER involvement in physiology and disease, providing the first evidence of its pronociceptive effects at central levels and characterizing some of the underlying mechanisms... - Central role of Gq in the hypertrophic signal transduction of angiotensin II in vascular smooth muscle cellsHaruhiko Ohtsu
Cardiovascular Research Center and Department of Physiology, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, PA 19140, USA
Endocrinology 149:3569-75. 2008..Vascular G(q) seems to be a critical target of intervention against cardiovascular diseases associated with the enhanced renin-angiotensin system... - Expression of estrogen receptor GPR30 in the rat spinal cord and in autonomic and sensory gangliaSiok L Dun
Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Neurosci Res 87:1610-9. 2009.... - Intracellular angiotensin II activates rat myometriumElena Deliu
Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennslyvania 19140, USA
Am J Physiol Cell Physiol 301:C559-65. 2011.... - G protein-coupled estrogen receptor 1-mediated effects in the rat myometriumAndrei A Tica
Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
Am J Physiol Cell Physiol 301:C1262-9. 2011..Taken together, our results indicate that GPER is expressed and functional in rat myometrium. GPER activation produces depolarization, elevates [Ca(2+)](i) and increases contractility in myometrial cells... - Endothelial nitric oxide synthase inhibits G12/13 and rho-kinase activated by the angiotensin II type-1 receptor: implication in vascular migrationHiroyuki Suzuki
Cardiovascular Research Center, Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, USA
Arterioscler Thromb Vasc Biol 29:217-24. 2009..Although, endothelial nitric oxide (NO) synthase (eNOS) is believed to antagonize vascular remodeling induced by the angiotensin II (AngII) type-1 receptor, the exact signaling mechanism remains unclear... - Intracellular endothelin type B receptor-driven Ca2+ signal elicits nitric oxide production in endothelial cellsElena Deliu
Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Biol Chem 287:41023-31. 2012..Endothelin-1 acts in an intracrine fashion on endolysosomal ET(B) to induce nitric oxide formation, thus modulating endothelial function... - Agonist-selective effects of opioid receptor ligands on cytosolic calcium concentration in rat striatal neuronsG Cristina Brailoiu
Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
Drug Alcohol Depend 123:277-81. 2012..Buprenorphine is an opioid receptor ligand whose mechanism of action is incompletely understood... - Nesfatin-1: distribution and interaction with a G protein-coupled receptor in the rat brainG Cristina Brailoiu
Department of Pharmacology, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, PA 19140, USA
Endocrinology 148:5088-94. 2007.... - Insulin-like peptide 5: expression in the mouse brain and mobilization of calciumSiok L Dun
Department of Pharmacology, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, Pennsylvania 19140, USA
Endocrinology 147:3243-8. 2006..The concentration of irINSL5 in the hypothalamic-pituitary axis suggests a neuroendocrine function of this insulin superfamily member... - An NAADP-gated two-pore channel targeted to the plasma membrane uncouples triggering from amplifying Ca2+ signalsEugen Brailoiu
Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Biol Chem 285:38511-6. 2010..We therefore uncouple TPC2 from amplification pathways and prove that it is a pore-forming subunit of an NAADP-gated Ca(2+) channel... - KiSS-1 expression and metastin-like immunoreactivity in the rat brainG Cristina Brailoiu
Department of Pharmacology, East Tennessee State University, Johnson City, Tennessee 37614, USA
J Comp Neurol 481:314-29. 2005..The results show that metastin is biologically active in rat central neurons, and its anatomical distribution suggests a possible role in nociception and autonomic and neuroendocrine functions... - Modulation of spontaneous transmitter release from the frog neuromuscular junction by interacting intracellular Ca(2+) stores: critical role for nicotinic acid-adenine dinucleotide phosphate (NAADP)Eugen Brailoiu
Department of Pharmacology, James H Quillen College of Medicine, East Tennessee State University, PO Box 70577, Johnson City, TN 37614, USA
Biochem J 373:313-8. 2003..Asynchronous neurotransmitter release may therefore involve functional coupling of endoplasmic reticulum Ca(2+) stores with distinct Ca(2+) stores targeted by NAADP... - Smooth muscle-associated protein 8: distribution and biological activity in the rat brainG Cristina Brailoiu
Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Neurosci Res 85:1789-96. 2007..This is the first evidence of irSMAP8 in a population of VP/OT-containing hypothalamic neurons in the rat, and the peptide is biologically active in hypothalamic neurons, as evidenced by mobilization of extracellular Ca2+... - Acidic NAADP-sensitive calcium stores in the endothelium: agonist-specific recruitment and role in regulating blood pressureG Cristina Brailoiu
Departments of Pharmacology, University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Biol Chem 285:37133-7. 2010..Our data extend the actions of NAADP to the endothelium both in vitro and in vivo, pointing to a previously unrecognized role for this messenger in controlling blood pressure... - Extra- and intracellular sphingosylphosphorylcholine promote spontaneous transmitter release from frog motor nerve endingsEugen Brailoiu
Department of Pharmacology, James H. Quillen College of Medicine, East Tennessee State University, PO Box 70577, Johnson City, TN 37614-1708, USA
Mol Pharmacol 63:1430-6. 2003..The result provides the first electrophysiological evidence that SPC can modulate transmitter release by an extra- or intracellular action at the frog motor nerve ending... - Nicotinic acid adenine dinucleotide phosphate potentiates neurite outgrowthEugen Brailoiu
Department of Pharmacology, Temple University Medical School, Philadelphia, PA 19140, USA
J Biol Chem 280:5646-50. 2005..Interplay between NAADP receptors and more established intracellular Ca(2+) channels may therefore play important signaling roles in the nervous system... - Expression and activity of cocaine- and amphetamine-regulated transcript peptide(1-39) in the ratSiok L Dun
Department of Pharmacology, Temple University School of Medicine, 3400 N Broad Street, Philadelphia, PA 19140, USA
Regul Pept 140:47-54. 2007.... - Inositol derivatives modulate spontaneous transmitter release at the frog neuromuscular junctionEugen Brailoiu
East Tennessee State University, Department of Pharmacology, James H. Quillen College of Medicine, P.O. Box 70577, Johnson City, TN 37614, USA
Neuropharmacology 45:691-701. 2003.... - Distribution and biological activity of obestatin in the ratSiok L Dun
Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Endocrinol 191:481-9. 2006.... - Photoaffinity labeling of nicotinic acid adenine dinucleotide phosphate (NAADP) targets in mammalian cellsYaping Lin-Moshier
Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
J Biol Chem 287:2296-307. 2012..This observation necessitates critical evaluation of current models of NAADP-triggered activation of the TPC family... - The G protein-coupled receptor GPR30 inhibits proliferation of estrogen receptor-positive breast cancer cellsEric A Ariazi
Fox Chase Cancer Center Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania, USA
Cancer Res 70:1184-94. 2010..Further, flow cytometry showed that G-1 blocked MCF-7 cell cycle progression at the G(1) phase. Thus, GPR30 antagonizes growth of ERalpha-positive breast cancer and may represent a new target to combat this disease... - An ancestral deuterostome family of two-pore channels mediates nicotinic acid adenine dinucleotide phosphate-dependent calcium release from acidic organellesEugen Brailoiu
Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Biol Chem 285:2897-901. 2010..Our data highlight the functional divergence of this novel gene family during deuterostome evolution and provide further evidence that NAADP mediates calcium release from acidic stores through activation of TPCs... 
NAADP-mediated channel 'chatter' in neurons of the rat medulla oblongataG Cristina Brailoiu
Department of Pharmacology, Temple University School of Medicine, 3420 N Broad St, Philadelphia, PA 19140, USA
Biochem J 419:91-7, 2 p following 97. 2009..Thus NAADP is capable of co-ordinating channels both within the cell interior and at the cell membrane representing a novel mechanism for excitation of central neurons...- Messenger-specific role for nicotinic acid adenine dinucleotide phosphate in neuronal differentiationEugen Brailoiu
Department of Pharmacology, Temple University Medical School, Philadelphia, Pennsylvania, USA
J Biol Chem 281:15923-8. 2006..Thus, differential recruitment of intracellular Ca2+-mobilizing messengers and their target Ca2+ stores may represent a robust means of maintaining stimulus fidelity in the control of Ca2+-dependent cell function... - Essential requirement for two-pore channel 1 in NAADP-mediated calcium signalingEugen Brailoiu
Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
J Cell Biol 186:201-9. 2009..Thus, TPC1 is critical for NAADP action and is likely the long sought after target channel for NAADP... - Acute exposure to ethanol potentiates human immunodeficiency virus type 1 Tat-induced Ca(2+) overload and neuronal death in cultured rat cortical neuronsEugen Brailoiu
Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Neurovirol 12:17-24. 2006..The result led the authors to conclude that alcohol exposure significantly potentiates Tat-induced calcium overload and neuronal death... - Surface-enhanced Raman spectroscopy as a tool for detecting Ca2+ mobilizing second messengers in cell extractsElina A Vitol
Department of Electrical and Computer Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USA
Anal Chem 82:6770-4. 2010..With a SERS sensor, NAADP can be detected in less than 1 min without any special sample preparation. To the best of our knowledge, this is the first demonstration of using SERS for calcium signaling applications... - Differential regulation of homocysteine transport in vascular endothelial and smooth muscle cellsXiaohua Jiang
Temple University School of Medicine, Department of Pharmacology, 3420 North Broad Street, Philadelphia, PA 19140, USA
Arterioscler Thromb Vasc Biol 27:1976-83. 2007..This study characterized and directly compared Hcy transport in cultured human aortic ECs (HAECs) and smooth muscle cells (HASMCs)... - Intracellular cannabinoid type 1 (CB1) receptors are activated by anandamideG Cristina Brailoiu
Department of Pharmacology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Biol Chem 286:29166-74. 2011..In addition, our results support a novel role for nicotinic acid-adenine dinucleotide phosphate in cannabinoid-induced calcium signaling... - ADAM17 mediates epidermal growth factor receptor transactivation and vascular smooth muscle cell hypertrophy induced by angiotensin IIHaruhiko Ohtsu
Cardiovascular Research Center, Temple University School of Medicine, 3420 N. Broad St, Philadelphia, PA 19140, USA
Arterioscler Thromb Vasc Biol 26:e133-7. 2006..These findings demonstrate a previously missing molecular mechanism by which Ang II promotes vascular remodeling... - Urotensin-II regulates intracellular calcium in dissociated rat spinal cord neuronsCatalin M Filipeanu
Department of Pharmacology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614, USA
J Neurochem 83:879-84. 2002..Present results demonstrate that U-II modulates [Ca2+]i in rat spinal cord neurons via protein kinase A cascade... - Carbon nanopipettes characterize calcium release pathways in breast cancer cellsMichael G Schrlau
Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA 19104, USA
Nanotechnology 19:325102. 2008..Their advantages over commonly used glass pipettes include smaller size, breakage and clogging resistance, and potential for multifunctionality such as in concurrent injection and electrical measurements... - Calmodulin increases transmitter release by mobilizing quanta at the frog motor nerve terminalEugen Brailoiu
Department of Pharmacology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee, TN 37614-1708, USA
Br J Pharmacol 137:719-27. 2002..The decreases in MEPP size and m produced by W7 were both reversed by addition of CaM. Our results suggest that CaM increases transmitter release by mobilizing synaptic vesicles at the frog motor nerve terminal... - Human type II pneumocyte chemotactic responses to CXCR3 activation are mediated by splice variant ARong Ji
Division of Pulmonary Disease and Critical Care Medicine, Department of Medicine, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
Am J Physiol Lung Cell Mol Physiol 294:L1187-96. 2008..Expression of CXCR3 in alveolar epithelial cells may be important in pneumocyte repair from injury... - Ultra low concentrations of morphine increase neurite outgrowth in cultured rat spinal cord and cerebral cortical neuronsEugen Brailoiu
Department of Pharmacology, James H. Quillen College of Medicine, East Tennessee State University, PO Box 70577, Johnson City, TN 37614, USA
Neurosci Lett 365:10-3. 2004..Pretreatment with naloxone did not prevent the morphine effect. The result shows that morphine at ultra low concentrations enhances neurite outgrowth of spinal and cortical neurons via a naloxone-independent mechanism... - G protein coupling and second messenger generation are indispensable for metalloprotease-dependent, heparin-binding epidermal growth factor shedding through angiotensin II type-1 receptorMizuo Mifune
Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Biol Chem 280:26592-9. 2005.... - Deregulation of microRNAs by HIV-1 Vpr protein leads to the development of neurocognitive disordersRuma Mukerjee
Department of Neurology, Molecular Studies of Neurodegenerative Diseases Laboratory, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
J Biol Chem 286:34976-85. 2011..Therefore, we conclude that Vpr plays a major role in neuronal dysfunction through deregulating microRNAs and their target genes, a phenomenon that could lead to the development of neurocognitive disorders... - Sphingosine 1-phosphate enhances spontaneous transmitter release at the frog neuromuscular junctionEugen Brailoiu
Department of Pharmacology, James H. Quillen College of Medicine, East Tennessee State University, PO Box 70577, Johnson City 37614, USA
Br J Pharmacol 136:1093-7. 2002..The result provides the first evidence that S1-P acting intracellularly but not extracellularly enhances spontaneous transmitter release at the frog neuromuscular junction...
Research Grants
- Role of nonnuclear estrogen receptor GPR30 in preganglionic vagal neuronsEugen Brailoiu; Fiscal Year: 2009..A better understanding of the mechanisms of action of estrogen is critical for the development of new treatments for cardiovascular disease. ..
- Role of nonnuclear estrogen receptor GPR30 in preganglionic vagal neuronsEugen Brailoiu; Fiscal Year: 2010..A better understanding of the mechanisms of action of estrogen is critical for the development of new treatments for cardiovascular disease. ..
- Role of nonnuclear estrogen receptor GPR30 in preganglionic vagal neuronsEugen Brailoiu; Fiscal Year: 2009..A better understanding of the mechanisms of action of estrogen is critical for the development of new treatments for cardiovascular disease. ..