Carlos A Castaneda
Affiliation: Syracuse University
Castaneda C, Chaturvedi A, Camara C, Curtis J, Krueger S, Fushman D. Linkage-specific conformational ensembles of non-canonical polyubiquitin chains. Phys Chem Chem Phys. 2016;18:5771-88 pubmed publisher
..These studies highlight the potential power of determining function from elucidation of conformational states. ..
Dao T, Martyniak B, Canning A, Lei Y, Colicino E, Cosgrove M, et al
. ALS-Linked Mutations Affect UBQLN2 Oligomerization and Phase Separation in a Position- and Amino Acid-Dependent Manner. Structure. 2019;: pubmed publisher
Riley J, Dao T, Castaneda C. Cancer Mutations in SPOP Put a Stop to Its Inter-compartmental Hops. Mol Cell. 2018;72:1-3 pubmed publisher
..2018) identify liquid-liquid phase separation as a mechanism for substrate-triggered localization of SPOP and ubiquitination machinery to different nuclear bodies and describe how cancer mutations disrupt this process. ..
Dao T, Kolaitis R, Kim H, O Donovan K, Martyniak B, Colicino E, et al
. Ubiquitin Modulates Liquid-Liquid Phase Separation of UBQLN2 via Disruption of Multivalent Interactions. Mol Cell. 2018;69:965-978.e6 pubmed publisher
..We postulate that UBQLN2 LLPS enables its recruitment to stress granules, where its interactions with ubiquitinated substrates reverse LLPS to enable shuttling of clients out of stress granules. ..
CastaÃ±eda C, Dixon E, Walker O, Chaturvedi A, Nakasone M, Curtis J, et al
. Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins. Structure. 2016;24:423-36 pubmed publisher
..Binding studies and mutagenesis confirmed this prediction, further highlighting structural/recognition versatility of polyubiquitins and the potential power of determining function from elucidation of conformational ensembles. ..