David R Mitchell

Summary

Affiliation: SUNY Upstate Medical University
Country: USA

Publications

  1. doi request reprint Twenty-five dyneins in Tetrahymena: A re-examination of the multidynein hypothesis
    David E Wilkes
    Department of Biology, Harvey Mudd College, Claremont, California 91711, USA
    Cell Motil Cytoskeleton 65:342-51. 2008
  2. pmc ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery
    Noveera T Ahmed
    Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY 13210, USA
    J Cell Biol 183:313-22. 2008
  3. pmc Mutations in axonemal dynein assembly factor DNAAF3 cause primary ciliary dyskinesia
    Hannah M Mitchison
    Molecular Medicine Unit, University College London Institute of Child Health, London, UK
    Nat Genet 44:381-9, S1-2. 2012
  4. pmc Polyglutamylation: the GLU that makes microtubules sticky
    David R Mitchell
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, 750 E Adams St, Syracuse, NY 13210, USA
    Curr Biol 20:R234-6. 2010
  5. pmc The evolution of eukaryotic cilia and flagella as motile and sensory organelles
    David R Mitchell
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, 750 E Adams St, Syracuse, New York 13210, USA
    Adv Exp Med Biol 607:130-40. 2007
  6. ncbi request reprint Reconstruction of the projection periodicity and surface architecture of the flagellar central pair complex
    David R Mitchell
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Cell Motil Cytoskeleton 55:188-99. 2003
  7. pmc Speculations on the evolution of 9+2 organelles and the role of central pair microtubules
    David R Mitchell
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, 750 E Adams St, Syracuse, NY 13210, USA
    Biol Cell 96:691-6. 2004
  8. pmc Bend propagation drives central pair rotation in Chlamydomonas reinhardtii flagella
    David R Mitchell
    Department of Cell and Developmental Biology, State University of New York Upstate Medical University, 750 E Adams St, Syracuse, NY 13210, USA
    J Cell Biol 166:709-15. 2004
  9. ncbi request reprint Orientation of the central pair complex during flagellar bend formation in Chlamydomonas
    David R Mitchell
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Cell Motil Cytoskeleton 56:120-9. 2003
  10. pmc Chlamydomonas flagellar outer row dynein assembly protein ODA7 interacts with both outer row and I1 inner row dyneins
    Judy Freshour
    Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 282:5404-12. 2007

Research Grants

Collaborators

  • Lotte B Pedersen
  • J L Rosenbaum
  • DOUGLAS COLE
  • Charles B Lindemann
  • David J Asai
  • Noveera T Ahmed
  • Chunlei Gao
  • Judy Freshour
  • Anudariya B Dean
  • Ruth Yokoyama
  • Hannah M Mitchison
  • David E Wilkes
  • Beth F Mitchell
  • Hui Zhang
  • Miriam Schmidts
  • Heymut Omran
  • Huda Mussaffi
  • Christopher O'Callaghan
  • Toshiki Yagi
  • Rob A Hirst
  • Maha Al Dabbagh
  • Philip L Beales
  • Niki T Loges
  • Athina Dritsoula
  • Hannah Blau
  • Heike Olbrich
  • Eddie M K Chung
  • Jeffrey D Amack
  • Guangliang Wang
  • Hadley E Watson
  • Ben F Lucker
  • Michael Feely
  • EILEEN O'TOOLE
  • Sudipto Ghosh

Detail Information

Publications18

  1. doi request reprint Twenty-five dyneins in Tetrahymena: A re-examination of the multidynein hypothesis
    David E Wilkes
    Department of Biology, Harvey Mudd College, Claremont, California 91711, USA
    Cell Motil Cytoskeleton 65:342-51. 2008
    ..An understanding of the relationships among the various dynein heavy chains is important when deducing functions across species...
  2. pmc ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery
    Noveera T Ahmed
    Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY 13210, USA
    J Cell Biol 183:313-22. 2008
    ..ODA16 appears to function as a cargo-specific adaptor between IFT particles and outer row dynein needed for efficient dynein transport into the flagellar compartment...
  3. pmc Mutations in axonemal dynein assembly factor DNAAF3 cause primary ciliary dyskinesia
    Hannah M Mitchison
    Molecular Medicine Unit, University College London Institute of Child Health, London, UK
    Nat Genet 44:381-9, S1-2. 2012
    ..These results support the existence of a conserved, multistep pathway for the cytoplasmic formation of assembly competent ciliary dynein complexes...
  4. pmc Polyglutamylation: the GLU that makes microtubules sticky
    David R Mitchell
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, 750 E Adams St, Syracuse, NY 13210, USA
    Curr Biol 20:R234-6. 2010
    ..New evidence suggests that some dynein motors will not function properly unless their track has been modified by a specific post-translational modification...
  5. pmc The evolution of eukaryotic cilia and flagella as motile and sensory organelles
    David R Mitchell
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, 750 E Adams St, Syracuse, New York 13210, USA
    Adv Exp Med Biol 607:130-40. 2007
    ....
  6. ncbi request reprint Reconstruction of the projection periodicity and surface architecture of the flagellar central pair complex
    David R Mitchell
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Cell Motil Cytoskeleton 55:188-99. 2003
    ..This appearance changes dramatically along the lateral edge of the C1 microtubule where material is arranged in rows of separate particles that may play a unique role in spoke-mediated regulation of flagellar dynein activity...
  7. pmc Speculations on the evolution of 9+2 organelles and the role of central pair microtubules
    David R Mitchell
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, 750 E Adams St, Syracuse, NY 13210, USA
    Biol Cell 96:691-6. 2004
    ..This paper presents hypothesized steps in this evolutionary process, and examples to support these hypotheses...
  8. pmc Bend propagation drives central pair rotation in Chlamydomonas reinhardtii flagella
    David R Mitchell
    Department of Cell and Developmental Biology, State University of New York Upstate Medical University, 750 E Adams St, Syracuse, NY 13210, USA
    J Cell Biol 166:709-15. 2004
    ..These results indicate that bend propagation drives central pair rotation and suggest that dynein regulation by central pair--radial spoke interactions involves passive central pair reorientation to changes in bend plane...
  9. ncbi request reprint Orientation of the central pair complex during flagellar bend formation in Chlamydomonas
    David R Mitchell
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Cell Motil Cytoskeleton 56:120-9. 2003
    ..These observations support a model in which central pair orientation in Chlamydomonas is linked to doublet-specific dynein activation, and bend propagation is linked to rotation of the central pair complex...
  10. pmc Chlamydomonas flagellar outer row dynein assembly protein ODA7 interacts with both outer row and I1 inner row dyneins
    Judy Freshour
    Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 282:5404-12. 2007
    ..Biochemical fractionation and rebinding studies support a model in which Oda7 participates in a previously uncharacterized structural link between inner and outer row dyneins...
  11. pmc ODA16p, a Chlamydomonas flagellar protein needed for dynein assembly
    Noveera T Ahmed
    Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY 13210, USA
    Mol Biol Cell 16:5004-12. 2005
    ....
  12. pmc Regulation of flagellar dynein activity by a central pair kinesin
    Ruth Yokoyama
    Department of Cell and Developmental Biology, State University of New York Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
    Proc Natl Acad Sci U S A 101:17398-403. 2004
    ..Klp1 therefore retains properties of a motor protein and is essential for normal flagellar motility. We hypothesize that Klp1 acts as a conformational switch to signal spoke-dependent control of dynein activity...
  13. pmc Oda16/Wdr69 is essential for axonemal dynein assembly and ciliary motility during zebrafish embryogenesis
    Chunlei Gao
    State University of New York Upstate Medical University, Department of Cell and Developmental Biology, Syracuse, New York 13210, USA
    Dev Dyn 239:2190-7. 2010
    ..Analysis of cilium ultrastructure revealed loss of outer dynein arms in morphant embryos. These results support a remarkable level of functional conservation for Oda16/Wdr69...
  14. pmc ATP production in Chlamydomonas reinhardtii flagella by glycolytic enzymes
    Beth F Mitchell
    Department of Biology, Le Moyne College, Syracuse, NY 13210, USA
    Mol Biol Cell 16:4509-18. 2005
    ..We conclude that in situ ATP synthesis throughout the flagellar compartment is essential for normal flagellar motility...
  15. pmc Chlamydomonas ODA10 is a conserved axonemal protein that plays a unique role in outer dynein arm assembly
    Anudariya B Dean
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY 13210
    Mol Biol Cell 24:3689-96. 2013
    ..The results suggest that ODA10p does not function as a part of a traditionally defined docking complex...
  16. pmc Cpc1, a Chlamydomonas central pair protein with an adenylate kinase domain
    Hui Zhang
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
    J Cell Sci 117:4179-88. 2004
    ....
  17. doi request reprint Analysis of the central pair microtubule complex in Chlamydomonas reinhardtii
    David R Mitchell
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York, USA
    Methods Cell Biol 92:197-213. 2009
    ..Here we describe genetic and biochemical methods of dissecting the Chlamydomonas central pair and electron microscopic methods useful to determine structure-function relationships in this complex...
  18. ncbi request reprint Evidence for axonemal distortion during the flagellar beat of Chlamydomonas
    Charles B Lindemann
    Department of Biological Sciences, Oakland University, Rochester, Michigan 48309, USA
    Cell Motil Cytoskeleton 64:580-9. 2007
    ..02 MPa. The possibility that the distortion of the axoneme during the beat could play a significant role in regulating dynein function is discussed...

Research Grants18

  1. MOLECULAR AND GENETIC ANALYSIS OF FLAGELLAR DYNEINS
    David R Mitchell; Fiscal Year: 2010
    ..They are also essential in the life cycles of many protozoan organisms that cause human diseases, such as trypanosomiasis, leishmaniasis, malaria, giardiasis, and trichosomiasis. ..
  2. MOLECULAR AND GENETIC ANALYSIS OF FLAGELLAR DYNEINS
    David Mitchell; Fiscal Year: 2003
    ..abstract_text> ..
  3. MOLECULAR AND GENETIC ANALYSIS OF FLAGELLAR DYNEINS
    David Mitchell; Fiscal Year: 2005
    ..Work on central pair kinesins will be extended to include all kinesins identified as central pair proteins, and knockdown strains will be used to screen for null mutations in central pair kinesin genes. ..
  4. MOLECULAR AND GENETIC ANALYSIS OF FLAGELLAR DYNEINS
    David Mitchell; Fiscal Year: 1993
    ..Previously-identified dynein assembly mutants will then be tested for allelism to these structural gene loci by a combination of RFLP mapping and transformation with cloned genes...
  5. MOLECULAR AND GENETIC ANALYSIS OF FLAGELLAR DYNEINS
    David Mitchell; Fiscal Year: 2007
    ..Work on central pair kinesins will be extended to include all kinesins identified as central pair proteins, and knockdown strains will be used to screen for null mutations in central pair kinesin genes. ..
  6. MOLECULAR AND GENETIC ANALYSIS OF FLAGELLAR DYNEINS
    David Mitchell; Fiscal Year: 2006
    ..Work on central pair kinesins will be extended to include all kinesins identified as central pair proteins, and knockdown strains will be used to screen for null mutations in central pair kinesin genes. ..
  7. MOLECULAR AND GENETIC ANALYSIS OF FLAGELLAR DYNEINS
    David Mitchell; Fiscal Year: 2002
    ..abstract_text> ..
  8. MOLECULAR AND GENETIC ANALYSIS OF FLAGELLAR DYNEINS
    David Mitchell; Fiscal Year: 2001
    ..abstract_text> ..
  9. MOLECULAR AND GENETIC ANALYSIS OF FLAGELLAR DYNEINS
    David Mitchell; Fiscal Year: 2000
    ..abstract_text> ..
  10. MOLECULAR AND GENETIC ANALYSIS OF FLAGELLAR DYNEINS
    David Mitchell; Fiscal Year: 1992
    ..Previously-identified dynein assembly mutants will then be tested for allelism to these structural gene loci by a combination of RFLP mapping and transformation with cloned genes...
  11. MOLECULAR AND GENETIC ANALYSIS OF FLAGELLAR DYNEINS
    David Mitchell; Fiscal Year: 1991
    ..Previously-identified dynein assembly mutants will then be tested for allelism to these structural gene loci by a combination of RFLP mapping and transformation with cloned genes...
  12. MOLECULAR AND GENETIC ANALYSIS OF FLAGELLAR DYNEINS
    David Mitchell; Fiscal Year: 2009
    ..Work on central pair kinesins will be extended to include all kinesins identified as central pair proteins, and knockdown strains will be used to screen for null mutations in central pair kinesin genes. ..