BARRY KNOX

Summary

Affiliation: SUNY Upstate Medical University
Country: USA

Publications

  1. doi request reprint Cooperative activation of Xenopus rhodopsin transcription by paired-like transcription factors
    Sarah E Reks
    Departments of Neuroscience and Physiology, Ophthalmology and Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, NY 13210, USA
    BMC Mol Biol 15:4. 2014
  2. pmc Bioinformatic identification of novel putative photoreceptor specific cis-elements
    Charles G Danko
    Department of Pharmacology, SUNY Upstate Medical University, Syracuse, NY, USA
    BMC Bioinformatics 8:407. 2007
  3. ncbi request reprint Heterologous expression of limulus rhodopsin
    Barry E Knox
    Department of Ophthalmology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 278:40493-502. 2003
  4. pmc Shedding light on cones
    Barry E Knox
    Department of Biochemistry and Department of Molecular Biology and Ophthalmology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    J Gen Physiol 127:355-8. 2006
  5. pmc Light responses in rods of vitamin A-deprived Xenopus
    Eduardo Solessio
    Department of Ophthalmology, SUNY Upstate Medical University, Center for Vision Research, Syracuse, New York 13210, USA
    Invest Ophthalmol Vis Sci 50:4477-86. 2009
  6. pmc Developmental regulation of calcium-dependent feedback in Xenopus rods
    Eduardo Solessio
    Center for Vision Research, Weiskotten Hall, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
    J Gen Physiol 124:569-85. 2004
  7. ncbi request reprint Circadian modulation of temporal properties of the rod pathway in larval Xenopus
    Eduardo Solessio
    Department of Ophthalmology and Center for Vision Research, Weiskotten Hall, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
    J Neurophysiol 92:2672-84. 2004
  8. ncbi request reprint Conserved transcriptional activators of the Xenopus rhodopsin gene
    S Leigh Whitaker
    Departments of Biochemistry and Molecular Biology and Ophthalmology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 279:49010-8. 2004
  9. ncbi request reprint Phototransduction by vertebrate ultraviolet visual pigments: protonation of the retinylidene Schiff base following photobleaching
    Abhiram Dukkipati
    Departments of Chemistry and Biology, Syracuse University, 111 College Place, Syracuse, NY 13244 4100, USA
    Biochemistry 41:9842-51. 2002
  10. ncbi request reprint The rod cGMP-phosphodiesterase beta-subunit promoter is a specific target for Sp4 and is not activated by other Sp proteins or CRX
    Leonid E Lerner
    Jules Stein Eye Institute, Department of Ophthalmology, UCLA School of Medicine, Los Angeles, California 90095, USA
    J Biol Chem 277:25877-83. 2002

Collaborators

  • Eduardo Solessio
  • Masahiro Kono
  • Robert Barlow
  • W Clay Smith
  • Leonid E Lerner
  • ROBERT RICHARDS BIRGE
  • David A Cameron
  • P D Calvert
  • JOSEPH BESHARSE
  • Vera A McIlvain
  • Sarah E Reks
  • Abhiram Dukkipati
  • Lavoisier S Ramos
  • Charles G Danko
  • R Bryan Sutton
  • Jon A Peet
  • S Leigh Whitaker
  • Ana Karin Kusnetzow
  • Kunnel R Babu
  • Lavoisier Ramos
  • Jennifer Lin-Jones
  • Vera McIlvain
  • Xuemei Zhu
  • Xinming Zhuo
  • Min Hsuan Chen
  • Arkady M Pertsov
  • Maochun Qin
  • Javier Navarro
  • Justin Robert
  • Sergey A Vishnivetskiy
  • Vsevolod V Gurevich
  • Dayanidhi Raman
  • Susan M Hanson
  • Alvina Bragin
  • Edward N Pugh
  • Sergei S Nikonov
  • Eric A Pierce
  • Shoba Mani
  • Xinyu Zhao
  • Ed Parker
  • Mike Wu
  • Beth Burnside
  • Sudha Babu
  • Cheryl M Craft
  • Anakarin Kusnetzow
  • Deepak Singh
  • Jaji Murage
  • Bo Ma

Detail Information

Publications18

  1. doi request reprint Cooperative activation of Xenopus rhodopsin transcription by paired-like transcription factors
    Sarah E Reks
    Departments of Neuroscience and Physiology, Ophthalmology and Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, NY 13210, USA
    BMC Mol Biol 15:4. 2014
    ..Cooperative action by these transcription factors activates rod-specific genes such as rhodopsin. However, a quantitative mechanistic explanation of transcriptional rate determinants is lacking...
  2. pmc Bioinformatic identification of novel putative photoreceptor specific cis-elements
    Charles G Danko
    Department of Pharmacology, SUNY Upstate Medical University, Syracuse, NY, USA
    BMC Bioinformatics 8:407. 2007
    ..Here, we use motif discovery algorithms to predict transcription factor binding sites involved in regulating the differences between murine rod and cone photoreceptor populations...
  3. ncbi request reprint Heterologous expression of limulus rhodopsin
    Barry E Knox
    Department of Ophthalmology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 278:40493-502. 2003
    ..This may be a general property of invertebrate opsins and may underlie some of the functional differences between invertebrate and vertebrate visual pigments...
  4. pmc Shedding light on cones
    Barry E Knox
    Department of Biochemistry and Department of Molecular Biology and Ophthalmology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    J Gen Physiol 127:355-8. 2006
  5. pmc Light responses in rods of vitamin A-deprived Xenopus
    Eduardo Solessio
    Department of Ophthalmology, SUNY Upstate Medical University, Center for Vision Research, Syracuse, New York 13210, USA
    Invest Ophthalmol Vis Sci 50:4477-86. 2009
    ..Contrasting phototransduction in vitamin A-deprived Xenopus rods with phototransduction in constitutively active mammalian rods may provide new understanding of the mechanisms that lead to retinal degeneration...
  6. pmc Developmental regulation of calcium-dependent feedback in Xenopus rods
    Eduardo Solessio
    Center for Vision Research, Weiskotten Hall, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
    J Gen Physiol 124:569-85. 2004
    ..We conclude that larval Xenopus maximize sensitivity at the expense of slower response kinetics while adults maximize response kinetics at the expense of sensitivity...
  7. ncbi request reprint Circadian modulation of temporal properties of the rod pathway in larval Xenopus
    Eduardo Solessio
    Department of Ophthalmology and Center for Vision Research, Weiskotten Hall, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
    J Neurophysiol 92:2672-84. 2004
    ..The temporal properties of the behaviorally measured rod mechanism correspond closely with those of the electrophysiologically measured retinal response, indicating that the rod signals are modulated at the level of the outer retina...
  8. ncbi request reprint Conserved transcriptional activators of the Xenopus rhodopsin gene
    S Leigh Whitaker
    Departments of Biochemistry and Molecular Biology and Ophthalmology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 279:49010-8. 2004
    ..Furthermore, activation of the endogenous rhodopsin gene was also observed in some animals, showing that XOtx5 and XL-Nrl can activate the promoter in native chromatin environment...
  9. ncbi request reprint Phototransduction by vertebrate ultraviolet visual pigments: protonation of the retinylidene Schiff base following photobleaching
    Abhiram Dukkipati
    Departments of Chemistry and Biology, Syracuse University, 111 College Place, Syracuse, NY 13244 4100, USA
    Biochemistry 41:9842-51. 2002
    ..We conclude that the MUV-visual pigment possesses an unprotonated retinylidene Schiff base in the dark state, and undergoes a protonation event during the photobleaching cascade...
  10. ncbi request reprint The rod cGMP-phosphodiesterase beta-subunit promoter is a specific target for Sp4 and is not activated by other Sp proteins or CRX
    Leonid E Lerner
    Jules Stein Eye Institute, Department of Ophthalmology, UCLA School of Medicine, Los Angeles, California 90095, USA
    J Biol Chem 277:25877-83. 2002
    ..We found that it binds purified TBP and TFIIB in gel mobility shift assays with cooperative enhancement of binding affinity...
  11. ncbi request reprint Nr2e3 and Nrl can reprogram retinal precursors to the rod fate in Xenopus retina
    Vera A McIlvain
    Department of Biochemistry and Molecular Biology and Ophthalmology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Dev Dyn 236:1970-9. 2007
    ..Misexpression of Nrl and Nr2e3 together were more effective than either transcription factor alone in directing precursors to the rod fate...
  12. ncbi request reprint Quantification of the cytoplasmic spaces of living cells with EGFP reveals arrestin-EGFP to be in disequilibrium in dark adapted rod photoreceptors
    Jon A Peet
    Temple University School of Medicine, 3400 North Broad Street, Philadelphia, PA 19140, USA
    J Cell Sci 117:3049-59. 2004
    ..Arrestin-EGFP mass was conserved during a large-scale, light-driven redistribution in which approximately 40% of the protein in the inner segment moved to the outer segment in less than 30 minutes...
  13. ncbi request reprint Crystal structure of cone arrestin at 2.3A: evolution of receptor specificity
    R Bryan Sutton
    Department of Neuroscience and Cell Biology, University of Texas Medical Branch, and Sealy Center for Molecular Science and Structural Biology, Galveston, TX 77555, USA
    J Mol Biol 354:1069-80. 2005
    ..Transient arrestin binding to the photopigment in cones may be responsible for the extremely rapid regeneration and reuse of the photopigment that is essential for cone function at high levels of illumination...
  14. pmc Perspectives on the counterion switch-induced photoactivation of the G protein-coupled receptor rhodopsin
    Robert R Birge
    Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, CT 06269, USA
    Proc Natl Acad Sci U S A 100:9105-7. 2003
  15. ncbi request reprint Regulation of photoactivation in vertebrate short wavelength visual pigments: protonation of the retinylidene Schiff base and a counterion switch
    Lavoisier S Ramos
    Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269, USA
    Biochemistry 46:5330-40. 2007
    ..We examine the relevance of this observation to the counterion switch mechanism of visual pigment activation...
  16. pmc Vertebrate ultraviolet visual pigments: protonation of the retinylidene Schiff base and a counterion switch during photoactivation
    Ana Karin Kusnetzow
    Departments of Chemistry and Molecular and Cell Biology, University of Connecticut, 55 North Eagleville Road, Storrs, CT 06269, USA
    Proc Natl Acad Sci U S A 101:941-6. 2004
    ..The findings with MUV are in close analogy to rhodopsin and provides further support for the importance of the counterion switch in the photoactivation of both rod and cone visual pigments...
  17. ncbi request reprint Mouse cone arrestin gene characterization: promoter targets expression to cone photoreceptors
    Xuemei Zhu
    The Mary D Allen Laboratory for Vision Research, Doheny Eye Institute, Department of Cell and Neurobiology, The Keck School of Medicine of the University of Southern California, Los Angeles, CA 90089 9112, USA
    FEBS Lett 524:116-22. 2002
    ....
  18. ncbi request reprint Disruption of kinesin II function using a dominant negative-acting transgene in Xenopus laevis rods results in photoreceptor degeneration
    Jennifer Lin-Jones
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA
    Invest Ophthalmol Vis Sci 44:3614-21. 2003
    ..In the current study, the role of kinesin II in rod photoreceptors was examined by expressing a dominant negative-acting transgene that disrupts kinesin II function in Xenopus laevis rods of transgenic tadpoles...

Research Grants20

  1. MOLECULAR MECHANISMS OF PHOTORECEPTOR FUNCTION
    BARRY KNOX; Fiscal Year: 2007
    ..Once the relevant signaling pathway(s) are identified, it may be possible to therapeutically target the signaling pathways to slow or block disease progression. ..
  2. STRUCTURE/FUNCTION OF SHORT WAVELENGTH VISUAL PIGMENTS
    Barry E Knox; Fiscal Year: 2010
    ..To accomplish this we will use an inducible heterologous over-expression system to produce milligram quantities of cone pigment. The information gained in this Aim will allow for protein crystallization trials in the future. ..
  3. MOLECULAR MECHANISMS OF PHOTORECEPTOR FUNCTION
    BARRY KNOX; Fiscal Year: 2004
    ....
  4. MOLECULAR MECHANISMS OF PHOTORECEPTOR FUNCTION
    Barry E Knox; Fiscal Year: 2010
    ..Once the relevant signaling pathway(s) are identified, it may be possible to therapeutically target the signaling pathways to slow or block disease progression. ..
  5. STRUCTURE/FUNCTION OF SHORT WAVELENGTH VISUAL PIGMENTS
    BARRY KNOX; Fiscal Year: 2006
    ..Moreover, the characterization of SWS1 functional regions will open the way to study pigment regeneration and folding that may be important in understanding the development and long-term health of cone photoreceptors. ..
  6. STRUCTURE/FUNCTION OF SHORT WAVELENGTH VISUAL PIGMENTS
    BARRY KNOX; Fiscal Year: 2000
    ....
  7. STRUCTURE/FUNCTION OF SHORT WAVELENGTH VISUAL PIGMENTS
    Barry E Knox; Fiscal Year: 2011
    ..To accomplish this we will use an inducible heterologous over-expression system to produce milligram quantities of cone pigment. The information gained in this Aim will allow for protein crystallization trials in the future. ..