Patricia Kane

Summary

Affiliation: SUNY Upstate Medical University
Country: USA

Publications

  1. pmc Targeting reversible disassembly as a mechanism of controlling V-ATPase activity
    Patricia M Kane
    Dept of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
    Curr Protein Pept Sci 13:117-23. 2012
  2. pmc Vacuolar H+-ATPase works in parallel with the HOG pathway to adapt Saccharomyces cerevisiae cells to osmotic stress
    Sheena Claire Li
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY, USA
    Eukaryot Cell 11:282-91. 2012
  3. pmc The long physiological reach of the yeast vacuolar H+-ATPase
    Patricia M Kane
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    J Bioenerg Biomembr 39:415-21. 2007
  4. ncbi request reprint Assembly and regulation of the yeast vacuolar H+-ATPase
    Patricia M Kane
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA
    J Bioenerg Biomembr 35:313-21. 2003
  5. ncbi request reprint Close-up and genomic views of the yeast vacuolar H+-ATPase
    Patricia M Kane
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams St, Syracuse, New York 13210, USA
    J Bioenerg Biomembr 37:399-403. 2005
  6. pmc The where, when, and how of organelle acidification by the yeast vacuolar H+-ATPase
    Patricia M Kane
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
    Microbiol Mol Biol Rev 70:177-91. 2006
  7. pmc Vacuolar and plasma membrane proton pumps collaborate to achieve cytosolic pH homeostasis in yeast
    Gloria A Martínez-Muñoz
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    J Biol Chem 283:20309-19. 2008
  8. ncbi request reprint Loss of vacuolar proton-translocating ATPase activity in yeast results in chronic oxidative stress
    Elena Milgrom
    Department of Biochemistry, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 282:7125-36. 2007
  9. pmc A genomic screen for yeast vacuolar membrane ATPase mutants
    Maria Sambade
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Genetics 170:1539-51. 2005
  10. ncbi request reprint The yeast vacuolar proton-translocating ATPase contains a subunit homologous to the Manduca sexta and bovine e subunits that is essential for function
    Maria Sambade
    Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 279:17361-5. 2004

Research Grants

Collaborators

  • Robert W West
  • S Wilkens
  • T R Hughes
  • Charles Boone
  • Anne M Smardon
  • Sheena Claire Li
  • Theodore T Diakov
  • Maureen Tarsio
  • Maria Sambade
  • Jason M Rizzo
  • Heba Diab
  • Gloria A Martínez-Muñoz
  • Masashi Ohira
  • Mali Liu
  • Kelly Keenan Curtis
  • Elena Milgrom
  • Colleen M H Charsky
  • Ainslie B Parsons
  • Zhenyu Zhang
  • Ester Cobb
  • Frank Middleton
  • Jianzhong Liu
  • Mercedes Alba
  • Zhijian Li
  • Chaoying Zhang
  • Grant W Brown
  • Huiming Ding
  • Bilal Sheikh
  • Renee L Brost
  • Colleen Charsky
  • Smitha A Francis
  • Yemisi Oluwatosin

Detail Information

Publications22

  1. pmc Targeting reversible disassembly as a mechanism of controlling V-ATPase activity
    Patricia M Kane
    Dept of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
    Curr Protein Pept Sci 13:117-23. 2012
    ..Tissue-specific V-ATPase inhibition is a potential therapy for osteoporosis and cancer; the possibility of exploiting reversible disassembly in design of novel V-ATPase inhibitors is discussed...
  2. pmc Vacuolar H+-ATPase works in parallel with the HOG pathway to adapt Saccharomyces cerevisiae cells to osmotic stress
    Sheena Claire Li
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY, USA
    Eukaryot Cell 11:282-91. 2012
    ..Together, these data suggest that the V-ATPase acts in parallel with the HOG pathway in order to mediate salt detoxification...
  3. pmc The long physiological reach of the yeast vacuolar H+-ATPase
    Patricia M Kane
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    J Bioenerg Biomembr 39:415-21. 2007
    ....
  4. ncbi request reprint Assembly and regulation of the yeast vacuolar H+-ATPase
    Patricia M Kane
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA
    J Bioenerg Biomembr 35:313-21. 2003
    ....
  5. ncbi request reprint Close-up and genomic views of the yeast vacuolar H+-ATPase
    Patricia M Kane
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams St, Syracuse, New York 13210, USA
    J Bioenerg Biomembr 37:399-403. 2005
    ..The combination of "close-up" biochemical views of V-ATPase structure and mechanism and "geomic" views of its functional reach promises to provide new insights into the physiological of V-ATPases...
  6. pmc The where, when, and how of organelle acidification by the yeast vacuolar H+-ATPase
    Patricia M Kane
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
    Microbiol Mol Biol Rev 70:177-91. 2006
    ..cerevisiae and also examines the relationship between biosynthesis and transport of V-ATPase and compartment-specific regulation of acidification...
  7. pmc Vacuolar and plasma membrane proton pumps collaborate to achieve cytosolic pH homeostasis in yeast
    Gloria A Martínez-Muñoz
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
    J Biol Chem 283:20309-19. 2008
    ....
  8. ncbi request reprint Loss of vacuolar proton-translocating ATPase activity in yeast results in chronic oxidative stress
    Elena Milgrom
    Department of Biochemistry, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 282:7125-36. 2007
    ..The results indicate that V-ATPase activity helps to protect cells from endogenous oxidative stress...
  9. pmc A genomic screen for yeast vacuolar membrane ATPase mutants
    Maria Sambade
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Genetics 170:1539-51. 2005
    ..A requirement for constitutive calcineurin activation may be the predominant physiological basis of the Vma- growth phenotype...
  10. ncbi request reprint The yeast vacuolar proton-translocating ATPase contains a subunit homologous to the Manduca sexta and bovine e subunits that is essential for function
    Maria Sambade
    Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 279:17361-5. 2004
    ..Based on this evidence, we propose that Vma9p is a genuine subunit of the yeast V-ATPase and that e subunits may be a functionally essential part of all eukaryotic V-ATPases...
  11. pmc The yeast lysosome-like vacuole: endpoint and crossroads
    Sheena Claire Li
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
    Biochim Biophys Acta 1793:650-63. 2009
    ..e. the "garbage dump" of the cell), and suggests instead that these compartments are unexpectedly dynamic and highly regulated...
  12. pmc Subunit interactions and requirements for inhibition of the yeast V1-ATPase
    Heba Diab
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 284:13316-25. 2009
    ..We propose that upon disassembly, the H subunit undergoes a conformational change that inhibits V(1)-ATPase activity and precludes V(0) interactions...
  13. ncbi request reprint Mutational analysis of the subunit C (Vma5p) of the yeast vacuolar H+-ATPase
    Kelly Keenan Curtis
    Department of Biochemistry, State University of New York, Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 277:8979-88. 2002
    ..The peripheral stalk of V-ATPases must balance the stability essential for productive catalysis with the dynamic instability involved in regulation; these three mutations may perturb that balance...
  14. ncbi request reprint RAVE is essential for the efficient assembly of the C subunit with the vacuolar H(+)-ATPase
    Anne M Smardon
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 282:26185-94. 2007
    ..Our data support a model where RAVE, through its interaction with subunit C, is facilitating V(1) peripheral stalk subunit interactions with V(0) during V-ATPase assembly...
  15. ncbi request reprint Diploids heterozygous for a vma13Delta mutation in Saccharomyces cerevisiae highlight the importance of V-ATPase subunit balance in supporting vacuolar acidification and silencing cytosolic V1-ATPase activity
    Jason M Rizzo
    Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 282:8521-32. 2007
    ..The results suggest that balancing levels of subunit H with those of other V-ATPase subunits is critical, both for allowing organelle acidification and for preventing unproductive hydrolysis of cytosolic ATP...
  16. ncbi request reprint The E and G subunits of the yeast V-ATPase interact tightly and are both present at more than one copy per V1 complex
    Masashi Ohira
    Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 281:22752-60. 2006
    ..J. (1997) J. Biol. Chem. 272, 26787-26793). These results are consistent with recent models showing at least two peripheral stalks connecting the V1 and V0 sectors of the V-ATPase and would allow both stalks to be based on an EG dimer...
  17. pmc Structural and functional separation of the N- and C-terminal domains of the yeast V-ATPase subunit H
    Mali Liu
    Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 280:36978-85. 2005
    ..The N-terminal domain alone is sufficient for activation of ATP hydrolysis in V(1), but the C-terminal domain is essential for proper communication between the V(1) and V(o) sectors...
  18. pmc Regulation of vacuolar proton-translocating ATPase activity and assembly by extracellular pH
    Theodore T Diakov
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13219, USA
    J Biol Chem 285:23771-8. 2010
    ..These results also suggest that integrated pH and metabolic inputs determine the final assembly state and activity of the V-ATPase...
  19. ncbi request reprint The RAVE complex is essential for stable assembly of the yeast V-ATPase
    Anne M Smardon
    Department of Biochemistry and Molecular Biology, State University of New York, Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 277:13831-9. 2002
    ....
  20. ncbi request reprint Novel vacuolar H+-ATPase complexes resulting from overproduction of Vma5p and Vma13p
    Kelly Keenan Curtis
    Department of Biochemistry and Molecular Biology, State University of New York, Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 277:2716-24. 2002
    ..Overproduced Vma5p associated with cytosolic V(1) complexes; this association may cause the lethality...
  21. ncbi request reprint Yeast V1-ATPase: affinity purification and structural features by electron microscopy
    Zhenyu Zhang
    Department of Biochemistry, University of California Riverside, Riverside, CA 92521, USA
    J Biol Chem 278:47299-306. 2003
    ..Vasilyeva, E., and Forgac, M. (1999) J. Biol. Chem. 274, 31804-31810) indicates that the structure of the isolated V1 from yeast is very similar to the structure of the V1 domain in the intact V-ATPase complex...
  22. ncbi request reprint Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathways
    Ainslie B Parsons
    Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5G 1L6, Canada
    Nat Biotechnol 22:62-9. 2004
    ..This method thus provides a powerful means for inferring mechanism of action...

Research Grants22

  1. A Skpl-containing Complex Regulating V-ATPase Activity
    Patricia Kane; Fiscal Year: 2005
    ..abstract_text> ..
  2. Molecular & Cellular Bioenergetics Gordon Conf. 2005
    Patricia Kane; Fiscal Year: 2005
    ..The format of the Gordon Conference is designed to maximize opportunities for discussion and to encourage presentation of the latest results in the field. ..
  3. Subunit Structure and Function in Vacuolar H+-ATPase
    Patricia Kane; Fiscal Year: 2006
    ....
  4. Subunit Structure and Function in Vacuolar H+-ATPases
    Patricia Kane; Fiscal Year: 2007
    ..These experiments may help explain the far-reaching physiological defects of yeast mutants lacking V-ATPase activity. We will also examine the activity of the V-ATPase itself in response to measured cytosolic and vacuolar pH changes. ..
  5. SUBUNIT STRUCTURE/FUNCTION IN VACUOLAR H+ ATPASES
    Patricia Kane; Fiscal Year: 2002
    ....
  6. Subunit Structure and Function in Vacuolar H+-ATPases
    Patricia M Kane; Fiscal Year: 2010
    ..These experiments may help explain the far-reaching physiological defects of yeast mutants lacking V-ATPase activity. We will also examine the activity of the V-ATPase itself in response to measured cytosolic and vacuolar pH changes. ..