Research Topics
| Patricia KaneSummaryAffiliation: SUNY Upstate Medical University Country: USA Publications
Research Grants
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Detail Information
Publications
Targeting reversible disassembly as a mechanism of controlling V-ATPase activityPatricia M Kane
Dept of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
Curr Protein Pept Sci 13:117-23. 2012..Tissue-specific V-ATPase inhibition is a potential therapy for osteoporosis and cancer; the possibility of exploiting reversible disassembly in design of novel V-ATPase inhibitors is discussed...- Vacuolar H+-ATPase works in parallel with the HOG pathway to adapt Saccharomyces cerevisiae cells to osmotic stressSheena Claire Li
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY, USA
Eukaryot Cell 11:282-91. 2012..Together, these data suggest that the V-ATPase acts in parallel with the HOG pathway in order to mediate salt detoxification... - The long physiological reach of the yeast vacuolar H+-ATPasePatricia M Kane
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
J Bioenerg Biomembr 39:415-21. 2007.... 
Assembly and regulation of the yeast vacuolar H+-ATPasePatricia M Kane
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA
J Bioenerg Biomembr 35:313-21. 2003....- Close-up and genomic views of the yeast vacuolar H+-ATPasePatricia M Kane
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams St, Syracuse, New York 13210, USA
J Bioenerg Biomembr 37:399-403. 2005..The combination of "close-up" biochemical views of V-ATPase structure and mechanism and "geomic" views of its functional reach promises to provide new insights into the physiological of V-ATPases... - The where, when, and how of organelle acidification by the yeast vacuolar H+-ATPasePatricia M Kane
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
Microbiol Mol Biol Rev 70:177-91. 2006..cerevisiae and also examines the relationship between biosynthesis and transport of V-ATPase and compartment-specific regulation of acidification... - Vacuolar and plasma membrane proton pumps collaborate to achieve cytosolic pH homeostasis in yeastGloria A Martínez-Muñoz
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
J Biol Chem 283:20309-19. 2008.... - Loss of vacuolar proton-translocating ATPase activity in yeast results in chronic oxidative stressElena Milgrom
Department of Biochemistry, SUNY Upstate Medical University, Syracuse, New York 13210, USA
J Biol Chem 282:7125-36. 2007..The results indicate that V-ATPase activity helps to protect cells from endogenous oxidative stress... - A genomic screen for yeast vacuolar membrane ATPase mutantsMaria Sambade
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
Genetics 170:1539-51. 2005..A requirement for constitutive calcineurin activation may be the predominant physiological basis of the Vma- growth phenotype... - The yeast vacuolar proton-translocating ATPase contains a subunit homologous to the Manduca sexta and bovine e subunits that is essential for functionMaria Sambade
Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
J Biol Chem 279:17361-5. 2004..Based on this evidence, we propose that Vma9p is a genuine subunit of the yeast V-ATPase and that e subunits may be a functionally essential part of all eukaryotic V-ATPases... - The yeast lysosome-like vacuole: endpoint and crossroadsSheena Claire Li
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
Biochim Biophys Acta 1793:650-63. 2009..e. the "garbage dump" of the cell), and suggests instead that these compartments are unexpectedly dynamic and highly regulated... - Subunit interactions and requirements for inhibition of the yeast V1-ATPaseHeba Diab
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
J Biol Chem 284:13316-25. 2009..We propose that upon disassembly, the H subunit undergoes a conformational change that inhibits V(1)-ATPase activity and precludes V(0) interactions... - Mutational analysis of the subunit C (Vma5p) of the yeast vacuolar H+-ATPaseKelly Keenan Curtis
Department of Biochemistry, State University of New York, Upstate Medical University, Syracuse, New York 13210, USA
J Biol Chem 277:8979-88. 2002..The peripheral stalk of V-ATPases must balance the stability essential for productive catalysis with the dynamic instability involved in regulation; these three mutations may perturb that balance... - RAVE is essential for the efficient assembly of the C subunit with the vacuolar H(+)-ATPaseAnne M Smardon
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
J Biol Chem 282:26185-94. 2007..Our data support a model where RAVE, through its interaction with subunit C, is facilitating V(1) peripheral stalk subunit interactions with V(0) during V-ATPase assembly... - Diploids heterozygous for a vma13Delta mutation in Saccharomyces cerevisiae highlight the importance of V-ATPase subunit balance in supporting vacuolar acidification and silencing cytosolic V1-ATPase activityJason M Rizzo
Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
J Biol Chem 282:8521-32. 2007..The results suggest that balancing levels of subunit H with those of other V-ATPase subunits is critical, both for allowing organelle acidification and for preventing unproductive hydrolysis of cytosolic ATP... - The E and G subunits of the yeast V-ATPase interact tightly and are both present at more than one copy per V1 complexMasashi Ohira
Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
J Biol Chem 281:22752-60. 2006..J. (1997) J. Biol. Chem. 272, 26787-26793). These results are consistent with recent models showing at least two peripheral stalks connecting the V1 and V0 sectors of the V-ATPase and would allow both stalks to be based on an EG dimer... - Structural and functional separation of the N- and C-terminal domains of the yeast V-ATPase subunit HMali Liu
Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
J Biol Chem 280:36978-85. 2005..The N-terminal domain alone is sufficient for activation of ATP hydrolysis in V(1), but the C-terminal domain is essential for proper communication between the V(1) and V(o) sectors... - Regulation of vacuolar proton-translocating ATPase activity and assembly by extracellular pHTheodore T Diakov
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13219, USA
J Biol Chem 285:23771-8. 2010..These results also suggest that integrated pH and metabolic inputs determine the final assembly state and activity of the V-ATPase... - The RAVE complex is essential for stable assembly of the yeast V-ATPaseAnne M Smardon
Department of Biochemistry and Molecular Biology, State University of New York, Upstate Medical University, Syracuse, New York 13210, USA
J Biol Chem 277:13831-9. 2002.... - Novel vacuolar H+-ATPase complexes resulting from overproduction of Vma5p and Vma13pKelly Keenan Curtis
Department of Biochemistry and Molecular Biology, State University of New York, Upstate Medical University, Syracuse, New York 13210, USA
J Biol Chem 277:2716-24. 2002..Overproduced Vma5p associated with cytosolic V(1) complexes; this association may cause the lethality... - Yeast V1-ATPase: affinity purification and structural features by electron microscopyZhenyu Zhang
Department of Biochemistry, University of California Riverside, Riverside, CA 92521, USA
J Biol Chem 278:47299-306. 2003..Vasilyeva, E., and Forgac, M. (1999) J. Biol. Chem. 274, 31804-31810) indicates that the structure of the isolated V1 from yeast is very similar to the structure of the V1 domain in the intact V-ATPase complex... - Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathwaysAinslie B Parsons
Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5G 1L6, Canada
Nat Biotechnol 22:62-9. 2004..This method thus provides a powerful means for inferring mechanism of action...
Research Grants
- A Skpl-containing Complex Regulating V-ATPase ActivityPatricia Kane; Fiscal Year: 2005..abstract_text> ..
- Molecular & Cellular Bioenergetics Gordon Conf. 2005Patricia Kane; Fiscal Year: 2005..The format of the Gordon Conference is designed to maximize opportunities for discussion and to encourage presentation of the latest results in the field. ..
- Subunit Structure and Function in Vacuolar H+-ATPasePatricia Kane; Fiscal Year: 2006....
- Subunit Structure and Function in Vacuolar H+-ATPasesPatricia Kane; Fiscal Year: 2007..These experiments may help explain the far-reaching physiological defects of yeast mutants lacking V-ATPase activity. We will also examine the activity of the V-ATPase itself in response to measured cytosolic and vacuolar pH changes. ..
- SUBUNIT STRUCTURE/FUNCTION IN VACUOLAR H+ ATPASESPatricia Kane; Fiscal Year: 2002....
- Subunit Structure and Function in Vacuolar H+-ATPasesPatricia M Kane; Fiscal Year: 2010..These experiments may help explain the far-reaching physiological defects of yeast mutants lacking V-ATPase activity. We will also examine the activity of the V-ATPase itself in response to measured cytosolic and vacuolar pH changes. ..