ECKARD A WIMMER

Summary

Affiliation: State University of New York
Country: USA

Publications

  1. pmc The test-tube synthesis of a chemical called poliovirus. The simple synthesis of a virus has far-reaching societal implications
    Eckard Wimmer
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY, USA
    EMBO Rep 7:S3-9. 2006
  2. pmc Virus attenuation by genome-scale changes in codon pair bias
    J Robert Coleman
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794, USA
    Science 320:1784-7. 2008
  3. doi request reprint Synthetic poliovirus and other designer viruses: what have we learned from them?
    Eckard Wimmer
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11790, USA
    Annu Rev Microbiol 65:583-609. 2011
  4. pmc Synthetic viruses: a new opportunity to understand and prevent viral disease
    Eckard Wimmer
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA
    Nat Biotechnol 27:1163-72. 2009
  5. ncbi request reprint Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template
    Jeronimo Cello
    Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794 5222, USA
    Science 297:1016-8. 2002
  6. pmc Functional dissection of a poliovirus cis-acting replication element [PV-cre(2C)]: analysis of single- and dual-cre viral genomes and proteins that bind specifically to PV-cre RNA
    Jiang Yin
    Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, 11794, USA
    J Virol 77:5152-66. 2003
  7. ncbi request reprint Analysis of the cloverleaf element in a human rhinovirus type 14/poliovirus chimera: correlation of subdomain D structure, ternary protein complex formation and virus replication
    Elizabeth Rieder
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY 11794 5222, USA
    J Gen Virol 84:2203-16. 2003
  8. pmc Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site
    Hidemi Toyoda
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11790, USA
    J Virol 81:10017-28. 2007
  9. pmc Live attenuated influenza virus vaccines by computer-aided rational design
    Steffen Mueller
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA
    Nat Biotechnol 28:723-6. 2010
  10. ncbi request reprint Growth phenotypes and biosafety profiles in poliovirus-receptor transgenic mice of recombinant oncolytic polio/human rhinoviruses
    Jeronimo Cello
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY 11794 5222, USA
    J Med Virol 80:352-9. 2008

Research Grants

  1. PICORNAVIRUS MOLECULAR BIOLOGY
    Eckard Wimmer; Fiscal Year: 2010
  2. Evolution of Human C-cluster Enteroviruses
    Eckard Wimmer; Fiscal Year: 2003
  3. EUROPIC america 2002: a conference for picornaviruses
    Eckard Wimmer; Fiscal Year: 2002
  4. POLIOVIRUS RECEPTOR AND INTERACTIONS WITH POLIOVIRUS
    Eckard Wimmer; Fiscal Year: 2001
  5. Structure and Function of IRES Elements
    Eckard Wimmer; Fiscal Year: 2006
  6. Synthetic Viral Genome Design for Rapid Vaccine Development
    Eckard Wimmer; Fiscal Year: 2010
  7. Poliovirus Receptor and Poliovirus Pathogenesis
    Eckard Wimmer; Fiscal Year: 2009
  8. Synthetic Viral Genome Design for Rapid Vaccine Development
    Eckard Wimmer; Fiscal Year: 2009
  9. PICORNAVIRUS MOLECULAR BIOLOGY
    Eckard Wimmer; Fiscal Year: 2007
  10. NOVEL CELLULAR REGULATORY FACTORS IN VIRAL TRANSLATION
    Eckard Wimmer; Fiscal Year: 2001

Collaborators

Detail Information

Publications31

  1. pmc The test-tube synthesis of a chemical called poliovirus. The simple synthesis of a virus has far-reaching societal implications
    Eckard Wimmer
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY, USA
    EMBO Rep 7:S3-9. 2006
  2. pmc Virus attenuation by genome-scale changes in codon pair bias
    J Robert Coleman
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794, USA
    Science 320:1784-7. 2008
    ..Polioviruses thus customized were used to immunize mice and provided protective immunity after challenge. This "death by a thousand cuts" strategy could be generally applicable to attenuating many kinds of viruses...
  3. doi request reprint Synthetic poliovirus and other designer viruses: what have we learned from them?
    Eckard Wimmer
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11790, USA
    Annu Rev Microbiol 65:583-609. 2011
    ..It also allows modification of viral genomes to an extent hitherto unthinkable. Recoding of poliovirus and influenza virus to develop new vaccine candidates and refactoring the phage T7 DNA genome are discussed as examples...
  4. pmc Synthetic viruses: a new opportunity to understand and prevent viral disease
    Eckard Wimmer
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA
    Nat Biotechnol 27:1163-72. 2009
    ..Although only a few applications of virus synthesis have been described as yet, key recent findings have been the resurrection of the 1918 influenza virus and the generation of codon- and codon pair-deoptimized polioviruses...
  5. ncbi request reprint Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template
    Jeronimo Cello
    Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794 5222, USA
    Science 297:1016-8. 2002
    ..Our results show that it is possible to synthesize an infectious agent by in vitro chemical-biochemical means solely by following instructions from a written sequence...
  6. pmc Functional dissection of a poliovirus cis-acting replication element [PV-cre(2C)]: analysis of single- and dual-cre viral genomes and proteins that bind specifically to PV-cre RNA
    Jiang Yin
    Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, 11794, USA
    J Virol 77:5152-66. 2003
    ..We have further demonstrated that the viral proteins 3CD(pro) and 3C(pro) form stable complexes with a transcript PV-cre(2C) RNA that can be considered critical for VPg uridylylation...
  7. ncbi request reprint Analysis of the cloverleaf element in a human rhinovirus type 14/poliovirus chimera: correlation of subdomain D structure, ternary protein complex formation and virus replication
    Elizabeth Rieder
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY 11794 5222, USA
    J Gen Virol 84:2203-16. 2003
    ..Biochemical assays demonstrated that the function of the CL element and the function of the cis-acting replication element in the 3D(pol)-3CD(pro)-dependent uridylylation of VPg are not linked...
  8. pmc Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site
    Hidemi Toyoda
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11790, USA
    J Virol 81:10017-28. 2007
    ..These data reveal a new essential function of the spacer between the CL and the IRES in poliovirus proliferation...
  9. pmc Live attenuated influenza virus vaccines by computer-aided rational design
    Steffen Mueller
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA
    Nat Biotechnol 28:723-6. 2010
    ..The method can be applied rapidly to any emerging influenza virus in its entirety, an advantage that is especially relevant when dealing with seasonal epidemics and pandemic threats, such as H5N1- or 2009-H1N1 influenza...
  10. ncbi request reprint Growth phenotypes and biosafety profiles in poliovirus-receptor transgenic mice of recombinant oncolytic polio/human rhinoviruses
    Jeronimo Cello
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY 11794 5222, USA
    J Med Virol 80:352-9. 2008
    ..These findings highlight the safety of intracerebral inoculation of PVS(RIPO) for the treatment of human glioma...
  11. pmc Putting synthesis into biology: a viral view of genetic engineering through de novo gene and genome synthesis
    Steffen Mueller
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794 5222, USA
    Chem Biol 16:337-47. 2009
    ..For this reason viruses are once again poised to lead the way in the budding field of synthetic biology--for better or worse...
  12. pmc Evidence for emergence of diverse polioviruses from C-cluster coxsackie A viruses and implications for global poliovirus eradication
    Ping Jiang
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY 11794 5222, USA
    Proc Natl Acad Sci U S A 104:9457-62. 2007
    ..In a PV-free world without PV neutralizing antibodies, contemporary C-CAV, like their ancestor(s), could be fertile ground for a PV-like agent to emerge by mutation...
  13. ncbi request reprint Oncolytic treatment and cure of neuroblastoma by a novel attenuated poliovirus in a novel poliovirus-susceptible animal model
    Hidemi Toyoda
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, New York 11794 5222, USA
    Cancer Res 67:2857-64. 2007
    ..We suggest that our novel attenuated oncolytic poliovirus is a promising candidate for effective oncolytic treatment of human neuroblastoma or other cancer even in the presence of present or induced antipolio immunity...
  14. pmc Reduction of the rate of poliovirus protein synthesis through large-scale codon deoptimization causes attenuation of viral virulence by lowering specific infectivity
    Steffen Mueller
    Department of Molecular Genetics and Microbiology, Life Sciences Building, Stony Brook University, Stony Brook, NY 11794 5222, USA
    J Virol 80:9687-96. 2006
    ....
  15. ncbi request reprint Interaction of the poliovirus receptor CD155 with the dynein light chain Tctex-1 and its implication for poliovirus pathogenesis
    Steffen Mueller
    Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, New York 11794, USA
    J Biol Chem 277:7897-904. 2002
    ..Association with Tctex-1 and, hence, with the dynein motor complex may offer an explanation for how poliovirus hijacks the cellular transport machinery to retrogradely ascend along the axon to the neuronal cell body...
  16. pmc cis-acting RNA signals in the NS5B C-terminal coding sequence of the hepatitis C virus genome
    Haekyung Lee
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794, USA
    J Virol 78:10865-77. 2004
    ..The other two stem-loops (SL-IV and SL-VII) of the NS5BCR domain were found to be important but not essential for colony formation by the subgenomic replicon. The precise function(s) of these cis-acting RNA elements is not known...
  17. pmc Mutation of a single conserved nucleotide between the cloverleaf and internal ribosome entry site attenuates poliovirus neurovirulence
    Nidia De Jesus
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Life Sciences Bldg, Stony Brook, NY 11794 5222, USA
    J Virol 79:14235-43. 2005
    ....
  18. ncbi request reprint Immunofluorescence analysis of poliovirus receptor expression in Peyer's patches of humans, primates, and CD155 transgenic mice: implications for poliovirus infection
    Akiko Iwasaki
    Department of Epidemiology and Public Health, School of Medicine, Yale University, New Haven, Connecticut, USA
    J Infect Dis 186:585-92. 2002
    ..This suggests that productive poliovirus infection of the gut is dependent on the expression of CD155 within the FAE, including the M cells, and on cells within Peyer's patches, most likely within germinal centers...
  19. ncbi request reprint A "slide-back" mechanism for the initiation of protein-primed RNA synthesis by the RNA polymerase of poliovirus
    Aniko V Paul
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, New York 11790, USA
    J Biol Chem 278:43951-60. 2003
    ..Furthermore, we observed that the spacing between G-1 and A-5 and the size of the loop affect the yield but not the nature of the VPg-linked products...
  20. pmc Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell-free in vitro translation-RNA replication system by viral protein 3CDpro
    David Franco
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY 11790, USA
    Virol J 2:86. 2005
    ..Our results indicate that the stimulation of RNA synthesis and virus maturation by 3CDpro in vitro is dependent on the presence of a VPg-linked RNA template...
  21. ncbi request reprint Complete protein linkage map between the P2 and P3 non-structural proteins of poliovirus
    Jiang Yin
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY 11794, USA
    J Gen Virol 88:2259-67. 2007
    ..The possible biological implication of the interactions observed in the yeast two-hybrid system will be discussed...
  22. pmc The C-terminal hydrophobic domain of hepatitis C virus RNA polymerase NS5B can be replaced with a heterologous domain of poliovirus protein 3A
    Haekyung Lee
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY 11794, USA
    J Virol 80:11343-54. 2006
    ..This suggests that specific interactions between the NS5B hydrophobic domain and other membrane-bound factors may not play a decisive role in HCV replication...
  23. pmc Stimulation of poliovirus synthesis in a HeLa cell-free in vitro translation-RNA replication system by viral protein 3CDpro
    David Franco
    Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY 11790, USA
    J Virol 79:6358-67. 2005
    ....
  24. pmc Tyrosine 3 of poliovirus terminal peptide VPg(3B) has an essential function in RNA replication in the context of its precursor protein, 3AB
    Ying Liu
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794, USA
    J Virol 81:5669-84. 2007
    ....
  25. ncbi request reprint Poliovirus and poliomyelitis: a tale of guts, brains, and an accidental event
    Steffen Mueller
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794, USA
    Virus Res 111:175-93. 2005
    ..This review will attempt to summarize our current knowledge of the molecular biology of poliovirus, its pathogenesis, as well as recent advances in the areas of cell and tissue tropism and mechanisms of central nervous system invasion...
  26. ncbi request reprint Expression of the human poliovirus receptor/CD155 gene is activated by sonic hedgehog
    David J Solecki
    Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, 11794, USA
    J Biol Chem 277:25697-702. 2002
    ..These data identify the CD155 gene as a transcriptional target of Shh, a finding that has significance for the normal function of CD155 during development and the expression of CD155 in neuroectodermal tumors...
  27. pmc Cis-acting RNA elements in human and animal plus-strand RNA viruses
    Ying Liu
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11790, USA
    Biochim Biophys Acta 1789:495-517. 2009
    ....
  28. ncbi request reprint Recruitment of nectin-3 to cell-cell junctions through trans-heterophilic interaction with CD155, a vitronectin and poliovirus receptor that localizes to alpha(v)beta3 integrin-containing membrane microdomains
    Steffen Mueller
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794, USA
    J Biol Chem 278:31251-60. 2003
    ....
  29. pmc Characterization of the New World monkey homologues of human poliovirus receptor CD155
    Shaukat Khan
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794 5222, USA
    J Virol 82:7167-79. 2008
    ....
  30. pmc Membrane topography of the hydrophobic anchor sequence of poliovirus 3A and 3AB proteins and the functional effect of 3A/3AB membrane association upon RNA replication
    Kentaro Fujita
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794 5222, USA
    Biochemistry 46:5185-99. 2007
    ..Moreover, in vitro uridylylation reactions showed that membrane-bound 3AB is not a substrate for 3D(pol), but free VPg released by cleavage of 3AB with proteinase 3CD(pro) could be uridylylated...
  31. ncbi request reprint Replication of a novel subgenomic HCV genotype 1a replicon expressing a puromycin resistance gene in Huh-7 cells
    Chengyu Liang
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11790, USA
    Virology 333:41-53. 2005
    ..The data identify PAC as an efficient selectable marker for studies of HCV replication, which may be useful with different genotypes in different host cell systems...

Research Grants44

  1. PICORNAVIRUS MOLECULAR BIOLOGY
    Eckard Wimmer; Fiscal Year: 2010
    ..Our understanding of the mechanism of encapsidation is expected to facilitate the development of new drugs to treat enteroviral infections. ..
  2. Evolution of Human C-cluster Enteroviruses
    Eckard Wimmer; Fiscal Year: 2003
    ..To assess this scenario we propose to establish a model system to test possibilities of speciation of C-cluster enteroviruses. ..
  3. EUROPIC america 2002: a conference for picornaviruses
    Eckard Wimmer; Fiscal Year: 2002
    ..The over will discuss the difficulties (circulation of vaccine strains, recent outbreak of poliomyelitis in the Western Hemisphere) that have emerged during the current, historic effect to globally eradicate poliovirus. ..
  4. POLIOVIRUS RECEPTOR AND INTERACTIONS WITH POLIOVIRUS
    Eckard Wimmer; Fiscal Year: 2001
    ..Finally, the genetic determinants of mouse neurovirulence of PV1(LS-a), a strain of poliovirus, will be studied with attention to genomic sequences outside the coding sequence for the capsid protein. ..
  5. Structure and Function of IRES Elements
    Eckard Wimmer; Fiscal Year: 2006
    ..We will investigate the molecular reason for this attenuation. Finally, we will determine whether we can exchange viral IRESs also IRESs of cellular mRNA. ..
  6. Synthetic Viral Genome Design for Rapid Vaccine Development
    Eckard Wimmer; Fiscal Year: 2010
    ..We describe an entirely novel and rapid method to generate anti-virus vaccine candidates that might prove applicable to most if not all human pathogenic viral systems. ..
  7. Poliovirus Receptor and Poliovirus Pathogenesis
    Eckard Wimmer; Fiscal Year: 2009
    ....
  8. Synthetic Viral Genome Design for Rapid Vaccine Development
    Eckard Wimmer; Fiscal Year: 2009
    ..We describe an entirely novel and rapid method to generate anti-virus vaccine candidates that might prove applicable to most if not all human pathogenic viral systems. ..
  9. PICORNAVIRUS MOLECULAR BIOLOGY
    Eckard Wimmer; Fiscal Year: 2007
    ..Our studies may lead to the development of new vaccines and of novel drugs directed against picornavirus infection...
  10. NOVEL CELLULAR REGULATORY FACTORS IN VIRAL TRANSLATION
    Eckard Wimmer; Fiscal Year: 2001
    ..The tool for studying the HCV IRES will be a novel poliovirus/HCV chimeric virus whose translation is solely controlled by the HCV IRES. ..
  11. VIRAL ONCOLOGY: RETROVIRUSES AND POLIOVIRUS
    Eckard Wimmer; Fiscal Year: 1980
    ..This, we hope, will elucidate some key problems in the molecular biology of picornaviruses, namely, the nature of the ribosome attachment site and the mechanism of replication of viral RNA. ..
  12. NOVEL CELLULAR REGULATORY FACTORS IN VIRAL TRANSLATION
    Eckard Wimmer; Fiscal Year: 1993
    ..They will also characterize cellular RNA binding proteins, a class of factors whose interaction with RNA and whose role in cellular metabolism is of central importance yet poorly understood...