Tzvi Tzfira

Summary

Affiliation: State University of New York
Country: USA

Publications

  1. ncbi request reprint Involvement of targeted proteolysis in plant genetic transformation by Agrobacterium
    Tzvi Tzfira
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794 5215, USA
    Nature 431:87-92. 2004
  2. ncbi request reprint pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants
    Tzvi Tzfira
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Plant Mol Biol 57:503-16. 2005
  3. pmc Involvement of KU80 in T-DNA integration in plant cells
    Jianxiong Li
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Proc Natl Acad Sci U S A 102:19231-6. 2005
  4. pmc Uncoupling of the functions of the Arabidopsis VIP1 protein in transient and stable plant genetic transformation by Agrobacterium
    Jianxiong Li
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Proc Natl Acad Sci U S A 102:5733-8. 2005
  5. ncbi request reprint Subcellular localization of interacting proteins by bimolecular fluorescence complementation in planta
    Vitaly Citovsky
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    J Mol Biol 362:1120-31. 2006
  6. doi request reprint Localizing protein-protein interactions by bimolecular fluorescence complementation in planta
    Vitaly Citovsky
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Methods 45:196-206. 2008
  7. pmc The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformation
    Benoît Lacroix
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    EMBO J 24:428-37. 2005
  8. ncbi request reprint Biological systems of the host cell involved in Agrobacterium infection
    Vitaly Citovsky
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794, USA
    Cell Microbiol 9:9-20. 2007
  9. ncbi request reprint Protein interactions involved in nuclear import of the Agrobacterium VirE2 protein in vivo and in vitro
    Vitaly Citovsky
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, New York 11794 5215, USA
    J Biol Chem 279:29528-33. 2004
  10. pmc C2H2 zinc finger-SET histone methyltransferase is a plant-specific chromatin modifier
    Alexander Krichevsky
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Dev Biol 303:259-69. 2007

Collaborators

Detail Information

Publications42

  1. ncbi request reprint Involvement of targeted proteolysis in plant genetic transformation by Agrobacterium
    Tzvi Tzfira
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794 5215, USA
    Nature 431:87-92. 2004
    ..The critical role of proteasomal degradation in Agrobacterium-mediated genetic transformation was also evident from inhibition of T-DNA expression by a proteasomal inhibitor...
  2. ncbi request reprint pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants
    Tzvi Tzfira
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Plant Mol Biol 57:503-16. 2005
    ....
  3. pmc Involvement of KU80 in T-DNA integration in plant cells
    Jianxiong Li
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Proc Natl Acad Sci U S A 102:19231-6. 2005
    ..This last result further supports the notion that integration of T-DNA molecules occurs through ds intermediates and requires active participation of the host's nonhomologous end-joining repair machinery...
  4. pmc Uncoupling of the functions of the Arabidopsis VIP1 protein in transient and stable plant genetic transformation by Agrobacterium
    Jianxiong Li
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Proc Natl Acad Sci U S A 102:5733-8. 2005
    ....
  5. ncbi request reprint Subcellular localization of interacting proteins by bimolecular fluorescence complementation in planta
    Vitaly Citovsky
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    J Mol Biol 362:1120-31. 2006
    ..We demonstrate the utility of these vectors for the analysis of specific protein-protein interactions in various cellular compartments, including the nucleus, plasmodesmata, and chloroplasts of different plant species and cell types...
  6. doi request reprint Localizing protein-protein interactions by bimolecular fluorescence complementation in planta
    Vitaly Citovsky
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Methods 45:196-206. 2008
    ....
  7. pmc The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformation
    Benoît Lacroix
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    EMBO J 24:428-37. 2005
    ....
  8. ncbi request reprint Biological systems of the host cell involved in Agrobacterium infection
    Vitaly Citovsky
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794, USA
    Cell Microbiol 9:9-20. 2007
    ....
  9. ncbi request reprint Protein interactions involved in nuclear import of the Agrobacterium VirE2 protein in vivo and in vitro
    Vitaly Citovsky
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, New York 11794 5215, USA
    J Biol Chem 279:29528-33. 2004
    ..Our results indicate that VIP1 functions as a molecular bridge between VirE2 and karyopherin alpha, allowing VirE2 to utilize the host cell nuclear import machinery even without being directly recognized by its components...
  10. pmc C2H2 zinc finger-SET histone methyltransferase is a plant-specific chromatin modifier
    Alexander Krichevsky
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Dev Biol 303:259-69. 2007
    ..Thus, AtSWP1 and AtCZS represent two main components of a co-repressor complex that fine tunes flowering and is unique to plants...
  11. ncbi request reprint Agrobacterium T-DNA integration: molecules and models
    Tzvi Tzfira
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Trends Genet 20:375-83. 2004
    ..In this article, we review our understanding of the specific function of these proteins and propose a detailed model for integration...
  12. ncbi request reprint Will you let me use your nucleus? How Agrobacterium gets its T-DNA expressed in the host plant cell
    Benoît Lacroix
    Department of Biochemistry and Cell Biology, State University of NY, Stony Brook, 11794 5212, USA
    Can J Physiol Pharmacol 84:333-45. 2006
    ..Finally, the chromatin-associated T-complex is uncoated from its escorting proteins prior to the conversion of the T-strand to a double-stranded form and its integration into the host genome...
  13. pmc Site-specific integration of Agrobacterium tumefaciens T-DNA via double-stranded intermediates
    Tzvi Tzfira
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794, USA
    Plant Physiol 133:1011-23. 2003
    ..Thus, T-strand molecules can be converted into double-stranded intermediates before their integration into the DSB sites within the host cell genome...
  14. pmc Increasing plant susceptibility to Agrobacterium infection by overexpression of the Arabidopsis nuclear protein VIP1
    Tzvi Tzfira
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Proc Natl Acad Sci U S A 99:10435-40. 2002
    ....
  15. ncbi request reprint A case of promiscuity: Agrobacterium's endless hunt for new partners
    Benoît Lacroix
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Trends Genet 22:29-37. 2006
    ..Moreover, better knowledge of the DNA-transfer mechanisms from bacteria to eukaryotic organisms can also help in understanding horizontal gene transfer--a driving force throughout biological evolution...
  16. ncbi request reprint Agrobacterium is not alone: gene transfer to plants by viruses and other bacteria
    Sang Min Chung
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794, USA
    Trends Plant Sci 11:1-4. 2006
    ....
  17. ncbi request reprint Exploring cargo transport mechanics in the type IV secretion systems
    Jianxiong Li
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Trends Microbiol 13:295-8. 2005
    ..These findings have expanded our knowledge and understanding of the still mostly obscure structure and function of the T4SSs...
  18. ncbi request reprint Partners-in-infection: host proteins involved in the transformation of plant cells by Agrobacterium
    Tzvi Tzfira
    Dept of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Trends Cell Biol 12:121-9. 2002
    ....
  19. ncbi request reprint A versatile vector system for multiple gene expression in plants
    Sang Min Chung
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, 11794, USA
    Trends Plant Sci 10:357-61. 2005
    ..However, new and versatile systems now exist that expand the user's choice to a large number of promoters and terminators, and various autofluorescent tags confer the ability to express multiple genes from a single transformation vector...
  20. pmc Increasing cloning possibilities using artificial zinc finger nucleases
    Vardit Zeevi
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 105:12785-90. 2008
    ..Of no less importance, we show that ZFNs can be designed to recognize and cleave existing DNA sequences for the custom-cloning of native target DNA molecules...
  21. doi request reprint Non-redundant functions of sHSP-CIs in acquired thermotolerance and their role in early seed development in Arabidopsis
    Mery Dafny-Yelin
    The Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel
    Plant Mol Biol 67:363-73. 2008
    ....
  22. doi request reprint Interlinking showy traits: co-engineering of scent and colour biosynthesis in flowers
    Michal Moyal Ben Zvi
    The Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
    Plant Biotechnol J 6:403-15. 2008
    ..The use of a regulatory factor that activates scent production creates a new biotechnological strategy for the metabolic architecture of fragrance, leading to the creation of novel genetic variability for breeding purposes...
  23. pmc Arabidopsis VIRE2 INTERACTING PROTEIN2 is required for Agrobacterium T-DNA integration in plants
    Ajith Anand
    Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401, USA
    Plant Cell 19:1695-708. 2007
    ....
  24. ncbi request reprint PSITE vectors for stable integration or transient expression of autofluorescent protein fusions in plants: probing Nicotiana benthamiana-virus interactions
    Romit Chakrabarty
    Department of Plant Pathology, University of Kentucky, Lexington 40546, USA
    Mol Plant Microbe Interact 20:740-50. 2007
    ..Finally, we show that pSITE vectors can be used for targeted gene expression in virus-infected cells, which should facilitate high-throughput characterization of protein dynamics in host-virus interactions...
  25. pmc pSAT RNA interference vectors: a modular series for multiple gene down-regulation in plants
    Mery Dafny-Yelin
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
    Plant Physiol 145:1272-81. 2007
    ..This vector system provides an important addition to the plant molecular biologist's toolbox, which will significantly facilitate the use of RNAi technology for analyses of multiple gene function in plant cells...
  26. doi request reprint The ongoing saga of Agrobacterium-host interactions
    Mery Dafny-Yelin
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Trends Plant Sci 13:102-5. 2008
    ..These findings join a long line of evidence showing how this clever bacterium has developed ways of using and abusing host biological systems for its own needs...
  27. pmc Advanced expression vector systems: new weapons for plant research and biotechnology
    Tzvi Tzfira
    Plant Physiol 145:1087-9. 2007
  28. pmc Delivery of multiple transgenes to plant cells
    Mery Dafny-Yelin
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
    Plant Physiol 145:1118-28. 2007
  29. ncbi request reprint Plant transformation by Agrobacterium tumefaciens: modulation of single-stranded DNA-VirE2 complex assembly by VirE1
    Daphna Frenkiel-Krispin
    Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 282:3458-64. 2007
    ..Based on this in vitro evidence, we suggest that the constrained volume of the VirB channel provides a natural site for the exchange of VirE2 binding from VirE1 to the T-strand...
  30. pmc The Agrobacterium-plant cell interaction. Taking biology lessons from a bug
    Tzvi Tzfira
    Plant Physiol 133:943-7. 2003
  31. ncbi request reprint Three-dimensional reconstruction of Agrobacterium VirE2 protein with single-stranded DNA
    Asmahan Abu-Arish
    Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 279:25359-63. 2004
    ..Coexisting with the helices, we also found VirE2 tetrameric ring structures. A two-dimensional average of the latter confirms the major features of the three-dimensional reconstruction...
  32. ncbi request reprint Reorganization of specific chromosomal domains and activation of silent genes in plant cells acquiring pluripotentiality
    Yigal Avivi
    Department of Plant Sciences, The Weizmann Institute of Science, Rehovot, Israel
    Dev Dyn 230:12-22. 2004
    ..This change leads to activation of silent genes whose products are involved in acquisition or maintenance of pluripotentiality and/or the ensuing fate of the cell...
  33. pmc Identification of Arabidopsis rat mutants
    Yanmin Zhu
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392, USA
    Plant Physiol 132:494-505. 2003
    ..Here, we present an update on the identification and characterization of these rat mutants...
  34. pmc The plant VirE2 interacting protein 1. a molecular link between the Agrobacterium T-complex and the host cell chromatin?
    Abraham Loyter
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Plant Physiol 138:1318-21. 2005
  35. ncbi request reprint Towards targeted mutagenesis and gene replacement in plants
    Tzvi Tzfira
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Trends Biotechnol 23:567-9. 2005
    ..These reports provide a foundation from which new technologies for site-specific genome alterations in plant species can be developed...
  36. ncbi request reprint On tracks and locomotives: the long route of DNA to the nucleus
    Tzvi Tzfira
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Trends Microbiol 14:61-3. 2006
    ..These studies shed light on the mechanism of Agrobacterium-mediated genetic transformation and could lead to new methods for the efficient transfection of synthetic DNA...
  37. ncbi request reprint CHRD, a plant member of the evolutionarily conserved YjgF family, influences photosynthesis and chromoplastogenesis
    Yael Leitner-Dagan
    The Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P O Box 12, Rehovot 76100, Israel
    Planta 225:89-102. 2006
    ..We suggest that CHRD's role in the angiosperm reproductive unit may be a rather recent evolutionary development; its original function may have been to protect the plant under stress conditions by preserving plastid functionality...
  38. ncbi request reprint Mammalian cells
    Tzvi Tzfira
    Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI, USA
    Methods Mol Biol 344:435-51. 2006
    ..We also demonstrate the use of mammalian cells for studies of the cellular components of the genetic transformation pathway...
  39. ncbi request reprint Agrobacterium-mediated genetic transformation of plants: biology and biotechnology
    Tzvi Tzfira
    Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI 48109, USA
    Curr Opin Biotechnol 17:147-54. 2006
    ....
  40. doi request reprint Probing interactions between plant virus movement proteins and nucleic acids
    Tzvi Tzfira
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109 1048, USA
    Methods Mol Biol 451:293-316. 2008
    ..We also describe protocols for purification of recombinant viral MPs over-expressed in bacteria and production of different DNA and RNA probes for these binding assays...
  41. doi request reprint Attacking the defenders: plant viruses fight back
    Avner Levy
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Trends Microbiol 16:194-7. 2008
    ..These reports provide novel insight into the mechanisms developed by viruses to disable the defense system of the plant...
  42. ncbi request reprint Functional analysis of the Cucumber mosaic virus 2b protein: pathogenicity and nuclear localization
    Yongzeng Wang
    Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
    J Gen Virol 85:3135-47. 2004
    ..Thus, the sequences corresponding to the two NLSs must have another role leading to pathogenicity enhancement...