Research Topics
| Tzvi TzfiraSummaryAffiliation: State University of New York Country: USA Publications
| Collaborators
|
Detail Information
Publications
Involvement of targeted proteolysis in plant genetic transformation by AgrobacteriumTzvi Tzfira
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794 5215, USA
Nature 431:87-92. 2004..The critical role of proteasomal degradation in Agrobacterium-mediated genetic transformation was also evident from inhibition of T-DNA expression by a proteasomal inhibitor...- pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plantsTzvi Tzfira
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
Plant Mol Biol 57:503-16. 2005.... - Involvement of KU80 in T-DNA integration in plant cellsJianxiong Li
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
Proc Natl Acad Sci U S A 102:19231-6. 2005..This last result further supports the notion that integration of T-DNA molecules occurs through ds intermediates and requires active participation of the host's nonhomologous end-joining repair machinery... - Uncoupling of the functions of the Arabidopsis VIP1 protein in transient and stable plant genetic transformation by AgrobacteriumJianxiong Li
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
Proc Natl Acad Sci U S A 102:5733-8. 2005.... - Subcellular localization of interacting proteins by bimolecular fluorescence complementation in plantaVitaly Citovsky
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, USA
J Mol Biol 362:1120-31. 2006..We demonstrate the utility of these vectors for the analysis of specific protein-protein interactions in various cellular compartments, including the nucleus, plasmodesmata, and chloroplasts of different plant species and cell types... - Localizing protein-protein interactions by bimolecular fluorescence complementation in plantaVitaly Citovsky
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
Methods 45:196-206. 2008.... - The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformationBenoît Lacroix
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
EMBO J 24:428-37. 2005.... - Biological systems of the host cell involved in Agrobacterium infectionVitaly Citovsky
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794, USA
Cell Microbiol 9:9-20. 2007.... - Protein interactions involved in nuclear import of the Agrobacterium VirE2 protein in vivo and in vitroVitaly Citovsky
Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, New York 11794 5215, USA
J Biol Chem 279:29528-33. 2004..Our results indicate that VIP1 functions as a molecular bridge between VirE2 and karyopherin alpha, allowing VirE2 to utilize the host cell nuclear import machinery even without being directly recognized by its components... - C2H2 zinc finger-SET histone methyltransferase is a plant-specific chromatin modifierAlexander Krichevsky
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
Dev Biol 303:259-69. 2007..Thus, AtSWP1 and AtCZS represent two main components of a co-repressor complex that fine tunes flowering and is unique to plants... - Agrobacterium T-DNA integration: molecules and modelsTzvi Tzfira
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
Trends Genet 20:375-83. 2004..In this article, we review our understanding of the specific function of these proteins and propose a detailed model for integration... - Will you let me use your nucleus? How Agrobacterium gets its T-DNA expressed in the host plant cellBenoît Lacroix
Department of Biochemistry and Cell Biology, State University of NY, Stony Brook, 11794 5212, USA
Can J Physiol Pharmacol 84:333-45. 2006..Finally, the chromatin-associated T-complex is uncoated from its escorting proteins prior to the conversion of the T-strand to a double-stranded form and its integration into the host genome... - Site-specific integration of Agrobacterium tumefaciens T-DNA via double-stranded intermediatesTzvi Tzfira
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794, USA
Plant Physiol 133:1011-23. 2003..Thus, T-strand molecules can be converted into double-stranded intermediates before their integration into the DSB sites within the host cell genome... - Increasing plant susceptibility to Agrobacterium infection by overexpression of the Arabidopsis nuclear protein VIP1Tzvi Tzfira
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
Proc Natl Acad Sci U S A 99:10435-40. 2002.... - A case of promiscuity: Agrobacterium's endless hunt for new partnersBenoît Lacroix
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
Trends Genet 22:29-37. 2006..Moreover, better knowledge of the DNA-transfer mechanisms from bacteria to eukaryotic organisms can also help in understanding horizontal gene transfer--a driving force throughout biological evolution... - Agrobacterium is not alone: gene transfer to plants by viruses and other bacteriaSang-Min Chung
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794, USA
Trends Plant Sci 11:1-4. 2006.... - Exploring cargo transport mechanics in the type IV secretion systemsJianxiong Li
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, USA
Trends Microbiol 13:295-8. 2005..These findings have expanded our knowledge and understanding of the still mostly obscure structure and function of the T4SSs... - Partners-in-infection: host proteins involved in the transformation of plant cells by AgrobacteriumTzvi Tzfira
Dept of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
Trends Cell Biol 12:121-9. 2002.... - A versatile vector system for multiple gene expression in plantsSang-Min Chung
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, 11794, USA
Trends Plant Sci 10:357-61. 2005..However, new and versatile systems now exist that expand the user's choice to a large number of promoters and terminators, and various autofluorescent tags confer the ability to express multiple genes from a single transformation vector... - Increasing cloning possibilities using artificial zinc finger nucleasesVardit Zeevi
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Proc Natl Acad Sci U S A 105:12785-90. 2008..Of no less importance, we show that ZFNs can be designed to recognize and cleave existing DNA sequences for the custom-cloning of native target DNA molecules... - Non-redundant functions of sHSP-CIs in acquired thermotolerance and their role in early seed development in ArabidopsisMery Dafny-Yelin
The Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel
Plant Mol Biol 67:363-73. 2008.... - Interlinking showy traits: co-engineering of scent and colour biosynthesis in flowersMichal Moyal Ben Zvi
The Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Plant Biotechnol J 6:403-15. 2008..The use of a regulatory factor that activates scent production creates a new biotechnological strategy for the metabolic architecture of fragrance, leading to the creation of novel genetic variability for breeding purposes... - Arabidopsis VIRE2 INTERACTING PROTEIN2 is required for Agrobacterium T-DNA integration in plantsAjith Anand
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401, USA
Plant Cell 19:1695-708. 2007.... - PSITE vectors for stable integration or transient expression of autofluorescent protein fusions in plants: probing Nicotiana benthamiana-virus interactionsRomit Chakrabarty
Department of Plant Pathology, University of Kentucky, Lexington 40546, USA
Mol Plant Microbe Interact 20:740-50. 2007..Finally, we show that pSITE vectors can be used for targeted gene expression in virus-infected cells, which should facilitate high-throughput characterization of protein dynamics in host-virus interactions... - pSAT RNA interference vectors: a modular series for multiple gene down-regulation in plantsMery Dafny Yelin
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
Plant Physiol 145:1272-81. 2007..This vector system provides an important addition to the plant molecular biologist's toolbox, which will significantly facilitate the use of RNAi technology for analyses of multiple gene function in plant cells... - The ongoing saga of Agrobacterium-host interactionsMery Dafny-Yelin
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Trends Plant Sci 13:102-5. 2008..These findings join a long line of evidence showing how this clever bacterium has developed ways of using and abusing host biological systems for its own needs... - Advanced expression vector systems: new weapons for plant research and biotechnologyTzvi Tzfira
Plant Physiol 145:1087-9. 2007 - Delivery of multiple transgenes to plant cellsMery Dafny-Yelin
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
Plant Physiol 145:1118-28. 2007 - Plant transformation by Agrobacterium tumefaciens: modulation of single-stranded DNA-VirE2 complex assembly by VirE1Daphna Frenkiel-Krispin
Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot 76100, Israel
J Biol Chem 282:3458-64. 2007..Based on this in vitro evidence, we suggest that the constrained volume of the VirB channel provides a natural site for the exchange of VirE2 binding from VirE1 to the T-strand... - The Agrobacterium-plant cell interaction. Taking biology lessons from a bugTzvi Tzfira
Plant Physiol 133:943-7. 2003 - Three-dimensional reconstruction of Agrobacterium VirE2 protein with single-stranded DNAAsmahan Abu-Arish
Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot 76100, Israel
J Biol Chem 279:25359-63. 2004..Coexisting with the helices, we also found VirE2 tetrameric ring structures. A two-dimensional average of the latter confirms the major features of the three-dimensional reconstruction... - Reorganization of specific chromosomal domains and activation of silent genes in plant cells acquiring pluripotentialityYigal Avivi
Department of Plant Sciences, The Weizmann Institute of Science, Rehovot, Israel
Dev Dyn 230:12-22. 2004..This change leads to activation of silent genes whose products are involved in acquisition or maintenance of pluripotentiality and/or the ensuing fate of the cell... - Identification of Arabidopsis rat mutantsYanmin Zhu
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA
Plant Physiol 132:494-505. 2003..Here, we present an update on the identification and characterization of these rat mutants... - The plant VirE2 interacting protein 1. a molecular link between the Agrobacterium T-complex and the host cell chromatin?Abraham Loyter
Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
Plant Physiol 138:1318-21. 2005 - Towards targeted mutagenesis and gene replacement in plantsTzvi Tzfira
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Trends Biotechnol 23:567-9. 2005..These reports provide a foundation from which new technologies for site-specific genome alterations in plant species can be developed... - On tracks and locomotives: the long route of DNA to the nucleusTzvi Tzfira
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Trends Microbiol 14:61-3. 2006..These studies shed light on the mechanism of Agrobacterium-mediated genetic transformation and could lead to new methods for the efficient transfection of synthetic DNA... - CHRD, a plant member of the evolutionarily conserved YjgF family, influences photosynthesis and chromoplastogenesisYael Leitner-Dagan
The Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P O Box 12, Rehovot 76100, Israel
Planta 225:89-102. 2006..We suggest that CHRD's role in the angiosperm reproductive unit may be a rather recent evolutionary development; its original function may have been to protect the plant under stress conditions by preserving plastid functionality... - Mammalian cellsTzvi Tzfira
Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI, USA
Methods Mol Biol 344:435-51. 2006..We also demonstrate the use of mammalian cells for studies of the cellular components of the genetic transformation pathway... - Agrobacterium-mediated genetic transformation of plants: biology and biotechnologyTzvi Tzfira
Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI 48109, USA
Curr Opin Biotechnol 17:147-54. 2006.... - Probing interactions between plant virus movement proteins and nucleic acidsTzvi Tzfira
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109 1048, USA
Methods Mol Biol 451:293-316. 2008..We also describe protocols for purification of recombinant viral MPs over-expressed in bacteria and production of different DNA and RNA probes for these binding assays... - Attacking the defenders: plant viruses fight backAvner Levy
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Trends Microbiol 16:194-7. 2008..These reports provide novel insight into the mechanisms developed by viruses to disable the defense system of the plant... - Functional analysis of the Cucumber mosaic virus 2b protein: pathogenicity and nuclear localizationYongzeng Wang
Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
J Gen Virol 85:3135-47. 2004..Thus, the sequences corresponding to the two NLSs must have another role leading to pathogenicity enhancement...