Research Topics
| Tatyana PestovaSummaryAffiliation: State University of New York Country: USA Publications
Research Grants
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Detail Information
Publications
Translational control by a small RNA: dendritic BC1 RNA targets the eukaryotic initiation factor 4A helicase mechanismDaisy Lin
The Robert F Furchgott Center for Neural and Behavioral Science, Department of Physiology and Pharmacology, State University of New York, Health Science Center at Brooklyn, 450 Clarkson Avenue, Brooklyn, NY 11203, USA
Mol Cell Biol 28:3008-19. 2008..In vivo, BC1 RNA represses translation of a reporter mRNA with 5' secondary structure. The eIF4A mechanism places BC RNAs in a central position to modulate protein synthesis in neurons...- The role of ABCE1 in eukaryotic posttermination ribosomal recyclingAndrey V Pisarev
Department of Cell Biology, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA
Mol Cell 37:196-210. 2010..Importantly, ABCE1 dissociates only post-TCs obtained with eRF1/eRF3 (or eRF1 alone), but not post-TCs obtained with puromycin in eRF1's absence... - Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recyclingMaxim A Skabkin
Department of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA
Genes Dev 24:1787-801. 2010..In addition to their role in initiation, Ligatin and MCT-1/DENR can promote release of deacylated tRNA and mRNA from recycled 40S subunits after ABCE1-mediated dissociation of post-termination ribosomes... - The roles of individual eukaryotic translation initiation factors in ribosomal scanning and initiation codon selectionTatyana V Pestova
Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, New York 11203 2098, USA
Genes Dev 16:2906-22. 2002.... - eIF2-dependent and eIF2-independent modes of initiation on the CSFV IRES: a common role of domain IITatyana V Pestova
Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
EMBO J 27:1060-72. 2008.... - Translation elongation after assembly of ribosomes on the Cricket paralysis virus internal ribosomal entry site without initiation factors or initiator tRNATatyana V Pestova
Department of Microbiology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
Genes Dev 17:181-6. 2003..Cycloheximide arrested ribosomes on the IRES only after two cycles of elongation, when the first deacylated tRNA reached the E-site after translocation from the A-site... 
Reconstitution of eukaryotic translation elongation in vitro following initiation by internal ribosomal entryTatyana V Pestova
Department of Microbiology and Immunology, State University of New York, Brooklyn, NY, USA
Methods 36:261-9. 2005....- Position of the CrPV IRES on the 40S subunit and factor dependence of IRES/80S ribosome assemblyTatyana V Pestova
Department of Microbiology and Immunology, State University of New York, 450 Clarkson Avenue, Brooklyn, New York 11203, USA
EMBO Rep 5:906-13. 2004..A connecting loop in the IRES's pseudoknot (PK) III domain, part of PK II and the entire domain containing PK I are solvent-exposed and occupy the E site and regions of the P site that are usually occupied by Met-tRNA(Met)(i)... - Position of eukaryotic initiation factor eIF1 on the 40S ribosomal subunit determined by directed hydroxyl radical probingIvan B Lomakin
Department of Microbiology and Immunology, SUNY Downstate Medical Center Brooklyn, New York 11203, USA
Genes Dev 17:2786-97. 2003.... - Eukaryotic initiation factors 4G and 4A mediate conformational changes downstream of the initiation codon of the encephalomyocarditis virus internal ribosomal entry siteVictoria G Kolupaeva
Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
Mol Cell Biol 23:687-98. 2003..We propose that these conformational changes prepare the region flanking AUG834 for productive binding of the ribosome... - In vitro reconstitution and biochemical characterization of translation initiation by internal ribosomal entryVictoria G Kolupaeva
Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, USA
Methods Enzymol 430:409-39. 2007.... - Assembly and analysis of eukaryotic translation initiation complexesAndrey V Pisarev
Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, USA
Methods Enzymol 430:147-77. 2007.... - Position of eukaryotic initiation factor eIF5B on the 80S ribosome mapped by directed hydroxyl radical probingAnett Unbehaun
Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY, USA
EMBO J 26:3109-23. 2007..Some of these changes could also occur upon binding of other translational GTPases, and may contribute to factor recognition... - Release of initiation factors from 48S complexes during ribosomal subunit joining and the link between establishment of codon-anticodon base-pairing and hydrolysis of eIF2-bound GTPAnett Unbehaun
Department of Microbiology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
Genes Dev 18:3078-93. 2004.... - Specific functional interactions of nucleotides at key -3 and +4 positions flanking the initiation codon with components of the mammalian 48S translation initiation complexAndrey V Pisarev
Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
Genes Dev 20:624-36. 2006.... - Direct functional interaction of initiation factor eIF4G with type 1 internal ribosomal entry sitesSylvain de Breyne
Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA
Proc Natl Acad Sci U S A 106:9197-202. 2009..These data suggest that fundamental aspects of the mechanisms of initiation on these unrelated classes of IRESs are similar... - In vitro reconstitution of eukaryotic translation reveals cooperativity between release factors eRF1 and eRF3Elena Z Alkalaeva
Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
Cell 125:1125-36. 2006..Cooperativity between eRF1 and eRF3 required the eRF3 binding C-terminal domain of eRF1... - The fidelity of translation initiation: reciprocal activities of eIF1, IF3 and YciHIvan B Lomakin
Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
EMBO J 25:196-210. 2006.... - Factor requirements for translation initiation on the Simian picornavirus internal ribosomal entry siteSylvain de Breyne
Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
RNA 14:367-80. 2008..Although these 40S/IRES/tRNA complexes could not form active 80S ribosomes, this constitutes a second difference between the SPV9 and HP IRESs. eIF1 destabilized the eIF2-independent ribosomal binding of initiator tRNA... - Recycling of eukaryotic posttermination ribosomal complexesAndrey V Pisarev
Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
Cell 131:286-99. 2007..Its activity is enhanced by eIFs 3j, 1, and 1A. eIF1 also mediates release of P site tRNA, whereas eIF3j ensures subsequent dissociation of mRNA... - Position of eukaryotic translation initiation factor eIF1A on the 40S ribosomal subunit mapped by directed hydroxyl radical probingYingpu Yu
Department of Microbiology and Immunology, SUNY Downstate Medical Center, NY, USA
Nucleic Acids Res 37:5167-82. 2009..In contrast, eIF1A-CTT appears to interfere with the P-site tRNA-head interaction in the 'closed' complex and is likely ejected from the P-site upon start codon recognition... - Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-associationVictoria G Kolupaeva
Department of Microbiology and Immunology, SUNY Downstate Medical Center, 450 Clarkson Ave, Box 44, Brooklyn, NY 11203, USA
RNA 11:470-86. 2005..The potential biological role of stimulation of eIF3/40S subunit interaction by an RNA cofactor in the absence of eIF2-ternary complex is discussed... - Ribosomal position and contacts of mRNA in eukaryotic translation initiation complexesAndrey V Pisarev
Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
EMBO J 27:1609-21. 2008.... - Coupled folding during translation initiationTatyana V Pestova
Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA
Cell 115:650-2. 2003.... - Kinetic analysis of the interaction of guanine nucleotides with eukaryotic translation initiation factor eIF5BVera P Pisareva
Department of Microbiology and Immunology, State University of New York Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203, USA
Biochemistry 46:2622-9. 2007..9 microM) and for mant-GMPPNP 1.5 times higher (Kd approximately 25.7 microM) than for mant-GTP, indicating that eIF5B tolerates modifications of the triphosphate moiety well... - Translation initiation on mammalian mRNAs with structured 5'UTRs requires DExH-box protein DHX29Vera P Pisareva
Department of Microbiology and Immunology, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA
Cell 135:1237-50. 2008..DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. NTP hydrolysis by DHX29 is strongly stimulated by 43S complexes and is required for DHX29's activity in promoting 48S complex formation... - Kinetic analysis of interaction of eukaryotic release factor 3 with guanine nucleotidesVera P Pisareva
Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
J Biol Chem 281:40224-35. 2006..Guanine nucleotide binding and exchange on eRF3, which therefore depends on stimulation by eRF1, is entirely different from that on prokaryotic RF3 and unusual among GTPases... - Small molecule derails translation initiationTatyana V Pestova
Nat Chem Biol 2:176-7. 2006 - Translation, interruptedTatyana V Pestova
Nat Struct Mol Biol 13:98-9. 2006 - Translation mechanism and regulation: old players, new concepts. Meeting on translational control and non-coding RNAMartin Holcik
Apoptosis Research Centre, Children's Hospital of Eastern Ontario Research Institute, 401 Smyth Road, Ottawa, Ontario K1H 8L1, Canada
EMBO Rep 8:639-43. 2007 - Initiation factor eIF5B catalyzes second GTP-dependent step in eukaryotic translation initiationJoon H Lee
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2716, USA
Proc Natl Acad Sci U S A 99:16689-94. 2002..Thus, in contrast to bacteria where the single GTPase IF2 is sufficient to catalyze translation initiation, eukaryotic cells require hydrolysis of GTP by both eIF2 and eIF5B to complete translation initiation... - Roles of the negatively charged N-terminal extension of Saccharomyces cerevisiae ribosomal protein S5 revealed by characterization of a yeast strain containing human ribosomal protein S5Oleksandr Galkin
Department of Biological, Geological and Environmental Sciences, Cleveland State University, Cleveland, Ohio 44115, USA
RNA 13:2116-28. 2007.... - Mapping the binding interface between human eukaryotic initiation factors 1A and 5B: a new interaction between old partnersAssen Marintchev
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Building C1, Room 112, 240 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 100:1535-40. 2003..The binding between the C termini of eIF1A and eIF5B has implications for eukaryote-specific mechanisms of recruitment and release of translation IFs from the ribosome...
Research Grants
- The mechanisms of eukaryotic translation termination and ribosomal recyclingTatyana V Pestova; Fiscal Year: 2010..Detailed understanding of the mechanism of termination will facilitate rational development of more efficacious agents for PSC suppression therapy. ..
- Ribosomal scanning and initiation codon selectionTatyana V Pestova; Fiscal Year: 2010..These studies will determine the molecular basis for key events in translation initiation, and its regulation by trans-acting factors, which is a prerequisite for the development of rational therapies to treat such diseases. ) ..
- Ribosomal scanning and initiation codon selectionTatyana Pestova; Fiscal Year: 2007..The resulting model will establish the relative positions of initiation factors, initiator tRNA and mRNA on the 40S subunit, and will reveal the structure of mRNA threading channel. ..
- The mechanisms of eukaryotic translation termination and ribosomal recyclingTatyana Pestova; Fiscal Year: 2007..Detailed understanding of the mechanism of termination will facilitate rational development of more efficacious agents for PSC suppression therapy. ..
- Mechanism of ribosomal subunit joining in eukaryotesTatyana Pestova; Fiscal Year: 2005....
- Ribosomal scanning and initiation codon selectionTatyana V Pestova; Fiscal Year: 2011..These studies will determine the molecular basis for key events in translation initiation, and its regulation by trans-acting factors, which is a prerequisite for the development of rational therapies to treat such diseases. ) ..