James Spudich

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. ncbi request reprint Myosin VI walks hand-over-hand along actin
    Zeynep Okten
    Stanford University School of Medicine, Beckman Center, 279 Campus Drive, Stanford, California 94305 5307, USA
    Nat Struct Mol Biol 11:884-7. 2004
  2. pmc Long single alpha-helical tail domains bridge the gap between structure and function of myosin VI
    Benjamin J Spink
    Department of Biochemistry, Stanford University, 279 Campus Drive, Stanford, California 94305, USA
    Nat Struct Mol Biol 15:591-7. 2008
  3. pmc Hypertrophic and dilated cardiomyopathy: four decades of basic research on muscle lead to potential therapeutic approaches to these devastating genetic diseases
    James A Spudich
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California Electronic address
    Biophys J 106:1236-49. 2014
  4. pmc Coupled myosin VI motors facilitate unidirectional movement on an F-actin network
    Sivaraj Sivaramakrishnan
    Department of Biochemistry, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 187:53-60. 2009
  5. pmc Structural and functional insights on the Myosin superfamily
    Divya P Syamaladevi
    National Centre for Biological Sciences NCBS TIFR, GKVK Campus, Bellary Road, Bangalore, India
    Bioinform Biol Insights 6:11-21. 2012
  6. pmc Rho kinase's role in myosin recruitment to the equatorial cortex of mitotic Drosophila S2 cells is for myosin regulatory light chain phosphorylation
    Sara O Dean
    Department of Biochemistry, Stanford University, Stanford, California, United States of America
    PLoS ONE 1:e131. 2006
  7. pmc Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod
    Daniel Hostetter
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA
    PLoS Biol 2:e356. 2004
  8. doi request reprint Molecular motors: a surprising twist in myosin VI translocation
    James A Spudich
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305, USA
    Curr Biol 18:R68-70. 2008
  9. pmc Myosin VI: an innovative motor that challenged the swinging lever arm hypothesis
    James A Spudich
    Department of Biochemistry, B400 Beckman Center, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    Nat Rev Mol Cell Biol 11:128-37. 2010
  10. pmc Molecular motors: forty years of interdisciplinary research
    James A Spudich
    Stanford University, Stanford, CA 94305, USA
    Mol Biol Cell 22:3936-9. 2011

Research Grants

  1. MYOSIN MOVEMENT IN VITRO-MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 2001
  2. CYTOKINESIS IN DICTYOSTELIUM--A MOLECULAR ANALYSIS
    James Spudich; Fiscal Year: 2000
  3. MYOSIN MOVEMENT IN VITRO-MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 2000
  4. HOMOLOGOUS RECOMBINATION IN DICTYOSTELIUM
    James Spudich; Fiscal Year: 1992
  5. HOMOLOGOUS RECOMBINATION IN DICTYOSTELIUM
    James Spudich; Fiscal Year: 1991
  6. HOMOLOGOUS RECOMBINATION IN DICTYOSTELIUM
    James Spudich; Fiscal Year: 1990
  7. CYTOKINESIS IN DICTYOSTELIUM--A MOLECULAR ANALYSIS
    James Spudich; Fiscal Year: 2001
  8. MYOSIN MOVEMENT IN VITRO-MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 2002
  9. MYOSIN MOVEMENT IN VITRO-MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 2009
  10. Myosin Movement in Vitro - Molecular Characterization
    James Spudich; Fiscal Year: 2007

Collaborators

  • H L Sweeney
  • RONALD ROCK
  • S Doniach
  • Douglas N Robinson
  • Scott Delp
  • Divya P Syamaladevi
  • Wenchuan Liang
  • Peggy M McMahon
  • T Hasson
  • E Egelman
  • Sivaraj Sivaramakrishnan
  • David Altman
  • Alexander R Dunn
  • Jung Chi Liao
  • Zev Bryant
  • Benjamin J Spink
  • Gandikota S Lakshmikanth
  • M Amanda Hartman
  • John F Dawson
  • Elena P Sablin
  • Thomas J Purcell
  • Qian Chen
  • Nathan C Geething
  • Susan Tang
  • Sara O Dean
  • Zeynep Okten
  • Daniel Hostetter
  • Sarah E Rice
  • Robert J Fletterick
  • Dina Finan
  • Peiying Chuan
  • Mary Williard Elting
  • Adelene Y L Sim
  • Jan Lipfert
  • Arturo De Lozanne
  • Satyajit Mayor
  • Jeanette P Schmidt
  • Debanjan Goswami
  • David Parker
  • Russ B Altman
  • Shirley Sutton
  • Sarah Rice
  • Sara Dean
  • Hans M Warrick
  • L Stirling Churchman
  • Ashutosh Tripathy
  • Margaret S VanLoock
  • Carl Morris

Detail Information

Publications35

  1. ncbi request reprint Myosin VI walks hand-over-hand along actin
    Zeynep Okten
    Stanford University School of Medicine, Beckman Center, 279 Campus Drive, Stanford, California 94305 5307, USA
    Nat Struct Mol Biol 11:884-7. 2004
    ..The most likely explanation for this result is a hand-over-hand walking mechanism. We hypothesize the existence of a flexible element that would allow the motor to bridge the observed 72-nm distance...
  2. pmc Long single alpha-helical tail domains bridge the gap between structure and function of myosin VI
    Benjamin J Spink
    Department of Biochemistry, Stanford University, 279 Campus Drive, Stanford, California 94305, USA
    Nat Struct Mol Biol 15:591-7. 2008
    ..Furthermore, the cargo binding domain seems to be folded back in the presence of the catalytic head, constituting a potential regulatory mechanism that inhibits dimerization...
  3. pmc Hypertrophic and dilated cardiomyopathy: four decades of basic research on muscle lead to potential therapeutic approaches to these devastating genetic diseases
    James A Spudich
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California Electronic address
    Biophys J 106:1236-49. 2014
    ....
  4. pmc Coupled myosin VI motors facilitate unidirectional movement on an F-actin network
    Sivaraj Sivaramakrishnan
    Department of Biochemistry, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 187:53-60. 2009
    ..Furthermore, several molecules of monomeric myosin VI, which are nonprocessive in single molecule assays, can coordinate to transport cargo with similar speeds as dimers...
  5. pmc Structural and functional insights on the Myosin superfamily
    Divya P Syamaladevi
    National Centre for Biological Sciences NCBS TIFR, GKVK Campus, Bellary Road, Bangalore, India
    Bioinform Biol Insights 6:11-21. 2012
    ..Structural deviations at coiled coil regions could be important for carrying out normal biological function of these proteins...
  6. pmc Rho kinase's role in myosin recruitment to the equatorial cortex of mitotic Drosophila S2 cells is for myosin regulatory light chain phosphorylation
    Sara O Dean
    Department of Biochemistry, Stanford University, Stanford, California, United States of America
    PLoS ONE 1:e131. 2006
    ..Rok phosphorylates several cellular proteins, including the myosin regulatory light chain (RLC)...
  7. pmc Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod
    Daniel Hostetter
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA
    PLoS Biol 2:e356. 2004
    ..Truncation analysis of AD-Cterm tail fragments shows that assembly is delicately balanced, resulting in assembled myosin-II molecules that are poised to disassemble due to the phosphorylation of only three threonines...
  8. doi request reprint Molecular motors: a surprising twist in myosin VI translocation
    James A Spudich
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305, USA
    Curr Biol 18:R68-70. 2008
    ..A recent study has revealed an unexpected change in conformation of the myosin VI converter domain, essential for twisting the lever arm through a approximately 180 degrees rotation to achieve a large step along actin...
  9. pmc Myosin VI: an innovative motor that challenged the swinging lever arm hypothesis
    James A Spudich
    Department of Biochemistry, B400 Beckman Center, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    Nat Rev Mol Cell Biol 11:128-37. 2010
    ..We now know that myosin VI does indeed operate by an unusual approximately 180 degrees lever arm swing and achieves its large step size using special structural features in its tail domain...
  10. pmc Molecular motors: forty years of interdisciplinary research
    James A Spudich
    Stanford University, Stanford, CA 94305, USA
    Mol Biol Cell 22:3936-9. 2011
    ..Molecular motor research is now at an exciting threshold of being able to enter into the realm of clinical applications...
  11. ncbi request reprint Molecular motors take tension in stride
    James A Spudich
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 126:242-4. 2006
    ..The single motor studies on dynein by Reck-Peterson et al (2006) described in this issue pave the way to understand molecular mechanisms used by this unique machine...
  12. pmc Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing
    Alexander R Dunn
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 107:7746-50. 2010
    ..This mechanism likely increases both the motor's processivity and its ability to act as an anchor under physiological conditions...
  13. ncbi request reprint The mechanism of myosin VI translocation and its load-induced anchoring
    David Altman
    Department of Physics, Stanford University, Stanford, CA 94305, USA
    Cell 116:737-49. 2004
    ..From our results, we propose a mechanism of myosin VI stepping that predicts a regulation through load of the motor's roles as transporter and anchor...
  14. pmc Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity
    Jung Chi Liao
    Department of Bioengineering, Stanford University, Stanford, CA 94305, USA
    J Mol Biol 392:862-7. 2009
    ..Further, we observed robust processive stepping of motors with artificial lever arms, demonstrating that processivity can arise without optimizing lever arm composition or mechanics...
  15. pmc The power stroke of myosin VI and the basis of reverse directionality
    Zev Bryant
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Proc Natl Acad Sci U S A 104:772-7. 2007
    ..We suggest that the lever arm rotates approximately 180 degrees between pre- and poststroke conformations...
  16. ncbi request reprint Identification of a minimal myosin Va binding site within an intrinsically unstructured domain of melanophilin
    Nathan C Geething
    Department of Biochemistry, Stanford University, Stanford, California 94041, USA
    J Biol Chem 282:21518-28. 2007
    ..We discuss the possible implications of protein intrinsic disorder in recruitment and maintenance of myosin Va on melanosome membranes...
  17. pmc Differential localization in cells of myosin II heavy chain kinases during cytokinesis and polarized migration
    Wenchuan Liang
    Department of Biochemistry, Stanford University School of Medicine, CA 94305 5307, USA
    BMC Cell Biol 3:19. 2002
    ..In this report we evaluate the biochemical properties of MHCK-C, and using fluorescence microscopy in living cells we examine the localization of GFP-labeled MHCK-A, -B, and -C in relation to GFP-myosin-II localization...
  18. ncbi request reprint Dynacortin is a novel actin bundling protein that localizes to dynamic actin structures
    Douglas N Robinson
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    J Biol Chem 277:9088-95. 2002
    ..These results indicate that cells have evolved different actin-filament cross-linking proteins with complementary cellular distributions that collaborate to orchestrate complex cell shape changes...
  19. pmc Quantitation of the distribution and flux of myosin-II during cytokinesis
    Douglas N Robinson
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    BMC Cell Biol 3:4. 2002
    ..To achieve this goal, the distribution and flux of the cell division machinery must be quantified. Here we report the first quantitative analysis of the distribution and flux of myosin-II, an essential element of the contractile ring...
  20. pmc Role of the lever arm in the processive stepping of myosin V
    Thomas J Purcell
    Department of Biochemistry, Stanford University Medical Center, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 99:14159-64. 2002
    ..These data show that for myosin V with its normal proximal tail domain, both heads and a long lever arm are required for large, processive steps...
  21. pmc Precise positioning of myosin VI on endocytic vesicles in vivo
    David Altman
    Department of Biochemistry, Stanford University Medical Center, Stanford, California, United States of America
    PLoS Biol 5:e210. 2007
    ..Our results are therefore consistent with vesicle-associated myosin VI existing as a processive dimer, capable of its known trafficking function...
  22. ncbi request reprint A flexible domain is essential for the large step size and processivity of myosin VI
    Ronald S Rock
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Mol Cell 17:603-9. 2005
    ..Based on these results, we present a mechanical model that describes stepping under an applied load...
  23. doi request reprint Principles of unconventional myosin function and targeting
    M Amanda Hartman
    Department of Biochemistry, Stanford University, Stanford, California 94305, USA
    Annu Rev Cell Dev Biol 27:133-55. 2011
    ....
  24. pmc Dynamic charge interactions create surprising rigidity in the ER/K alpha-helical protein motif
    Sivaraj Sivaramakrishnan
    Departments of Biochemistry, Applied Physics, and Physics, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 105:13356-61. 2008
    ..We propose that the significant rigidity of the ER/K alpha-helix can help regulate protein function, as a force transducer between protein subdomains...
  25. pmc Predicting allosteric communication in myosin via a pathway of conserved residues
    Susan Tang
    Department of Bioengineering, Stanford University, Stanford, CA 94305, USA
    J Mol Biol 373:1361-73. 2007
    ..We provide a refined, downloadable application and source code (on https://simtk.org) to share this tool with the wider community (https://simtk.org/home/allopathfinder)...
  26. ncbi request reprint Building and using optical traps to study properties of molecular motors
    Sarah E Rice
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305, USA
    Methods Enzymol 361:112-33. 2003
  27. ncbi request reprint Structure of an F-actin trimer disrupted by gelsolin and implications for the mechanism of severing
    John F Dawson
    Department of Biochemistry, Stanford University School of Medicine, California 94305, USA
    J Biol Chem 278:1229-38. 2003
    ..We also show that segment-1 of gelsolin is able to sever actin filaments, although the severing activity of segment-1 is significantly lower than full-length gelsolin...
  28. pmc Extending the absorbing boundary method to fit dwell-time distributions of molecular motors with complex kinetic pathways
    Jung Chi Liao
    Department of Bioengineering, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 104:3171-6. 2007
    ....
  29. ncbi request reprint Dynamics of the unbound head during myosin V processive translocation
    Alexander R Dunn
    Stanford University School of Medicine, Beckman Center, 279 Campus Drive, Stanford, California 94305 5307, USA
    Nat Struct Mol Biol 14:246-8. 2007
    ..We find that the unbound head rotates freely about the lever-arm junction, a trait that likely facilitates travel through crowded actin meshworks...
  30. pmc A mitotic kinesin-like protein required for normal karyokinesis, myosin localization to the furrow, and cytokinesis in Dictyostelium
    Gandikota S Lakshmikanth
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 101:16519-24. 2004
    ..Given these results, we hypothesize a mechanism for myosin II translocation to the furrow to set up the contractile ring...
  31. pmc Two important polymers cross paths
    James A Spudich
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Proc Natl Acad Sci U S A 101:15825-6. 2004
  32. ncbi request reprint Mechanics and regulation of cytokinesis
    Douglas N Robinson
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Curr Opin Cell Biol 16:182-8. 2004
    ..A genuine quantitative understanding of cytokinesis must include a thorough analysis of the mechanical properties of the cortex and how signals modify these properties to dictate a well-controlled, error-free cytokinesis...
  33. pmc How does ATP hydrolysis control actin's associations?
    Elena P Sablin
    Department of Biochemistry Biophysics, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 99:10945-7. 2002
  34. pmc The localization of inner centromeric protein (INCENP) at the cleavage furrow is dependent on Kif12 and involves interactions of the N terminus of INCENP with the actin cytoskeleton
    Qian Chen
    Section of Molecular Cell and Developmental Biology, University of Texas at Austin, Austin, TX 78712, USA
    Mol Biol Cell 18:3366-74. 2007
    ..Once INCENP reaches the equatorial cortex, it associates with the actin cytoskeleton where it then concentrates toward the end of cytokinesis...
  35. ncbi request reprint A crossbridge too far
    James A Spudich
    Nat Cell Biol 4:E8-10. 2002

Research Grants45

  1. MYOSIN MOVEMENT IN VITRO-MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 2001
    ..myosin mutants altered in lever arm length? 6) How does a single point mutation near the nucleotide binding site of the motor domain of myosin result in uncoupling of the actin-activated ATPase activity from mechanical movement? ..
  2. CYTOKINESIS IN DICTYOSTELIUM--A MOLECULAR ANALYSIS
    James Spudich; Fiscal Year: 2000
    ..Finally, synchronization of cytokinesis in populations of Dictyostelium cells for biochemical studies will be attempted. ..
  3. MYOSIN MOVEMENT IN VITRO-MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 2000
    ..myosin mutants altered in lever arm length? 6) How does a single point mutation near the nucleotide binding site of the motor domain of myosin result in uncoupling of the actin-activated ATPase activity from mechanical movement? ..
  4. HOMOLOGOUS RECOMBINATION IN DICTYOSTELIUM
    James Spudich; Fiscal Year: 1992
    ....
  5. HOMOLOGOUS RECOMBINATION IN DICTYOSTELIUM
    James Spudich; Fiscal Year: 1991
    ....
  6. HOMOLOGOUS RECOMBINATION IN DICTYOSTELIUM
    James Spudich; Fiscal Year: 1990
    ....
  7. CYTOKINESIS IN DICTYOSTELIUM--A MOLECULAR ANALYSIS
    James Spudich; Fiscal Year: 2001
    ..Finally, synchronization of cytokinesis in populations of Dictyostelium cells for biochemical studies will be attempted. ..
  8. MYOSIN MOVEMENT IN VITRO-MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 2002
    ..myosin mutants altered in lever arm length? 6) How does a single point mutation near the nucleotide binding site of the motor domain of myosin result in uncoupling of the actin-activated ATPase activity from mechanical movement? ..
  9. MYOSIN MOVEMENT IN VITRO-MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 2009
    ..Understanding how they work is therefore fundamental to understanding both their normal and pathologic behaviors. ..
  10. Myosin Movement in Vitro - Molecular Characterization
    James Spudich; Fiscal Year: 2007
    ..We will use multiple approaches to reveal regions that become exposed during its chemomechanical cycle. ..
  11. Myosin-II Dynamics and Cytokinesis
    James Spudich; Fiscal Year: 2005
    ..This method will allow dual visualization of myosin-II and other fluorescent-labeled cytokinesis-related proteins. ..
  12. Myosin Movement in Vitro - Molecular Characterization
    James Spudich; Fiscal Year: 2004
    ..We will use multiple approaches to reveal regions that become exposed during its chemomechanical cycle. ..
  13. Myosin-II Dynamics and Cytokinesis
    James Spudich; Fiscal Year: 2004
    ..This method will allow dual visualization of myosin-II and other fluorescent-labeled cytokinesis-related proteins. ..
  14. MYOSIN MOVEMENT IN VITRO-MOLECULAR CHARACTERIZATION
    James A Spudich; Fiscal Year: 2010
    ..Understanding how they work is therefore fundamental to understanding both their normal and pathologic behaviors. ..
  15. Myosin-II Dynamics and Cytokinesis
    James Spudich; Fiscal Year: 2003
    ..This method will allow dual visualization of myosin-II and other fluorescent-labeled cytokinesis-related proteins. ..
  16. MYOSIN MOVEMENT IN VITRO--MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 1993
    ..Such correlations of ATPase activity, force, and velocity should lead to a better understanding of the molecular basis of myosin movement...
  17. MYOSIN MOVEMENT IN VITRO: MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 1991
    ..We also propose to examine whether organelle movements in vivo involve myosin- driven translocations analogous to the myosin-coated bead movements on actin filaments in vitro...
  18. MYOSIN MOVEMENT IN VITRO--MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 1999
    ..How can we improve the feedback-enhanced laser trap system? 5. How will we use the improved feedback-enhanced laser trap system to study myosin function? ..
  19. CYTOKINESIS IN DICTYOSTELIUM--A MOLECULAR ANALYSIS
    James Spudich; Fiscal Year: 1999
    ..Finally, synchronization of cytokinesis in populations of Dictyostelium cells for biochemical studies will be attempted. ..
  20. MYOSIN MOVEMENT IN VITRO: MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 1990
    ..We also propose to examine whether organelle movements in vivo involve myosin- driven translocations analogous to the myosin-coated bead movements on actin filaments in vitro...
  21. MYOSIN MOVEMENT IN VITRO--MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 1992
    ..Such correlations of ATPase activity, force, and velocity should lead to a better understanding of the molecular basis of myosin movement...
  22. Myosin Movement in Vitro - Molecular Characterization
    James Spudich; Fiscal Year: 2006
    ..We will use multiple approaches to reveal regions that become exposed during its chemomechanical cycle. ..
  23. Myosin Movement in Vitro - Molecular Characterization
    James Spudich; Fiscal Year: 2005
    ..We will use multiple approaches to reveal regions that become exposed during its chemomechanical cycle. ..
  24. Myosin-II Dynamics and Cytokinesis
    James Spudich; Fiscal Year: 2002
    ..This method will allow dual visualization of myosin-II and other fluorescent-labeled cytokinesis-related proteins. ..
  25. MYOSIN MOVEMENT IN VITRO-MOLECULAR CHARACTERIZATION
    James Spudich; Fiscal Year: 2003
    ..myosin mutants altered in lever arm length? 6) How does a single point mutation near the nucleotide binding site of the motor domain of myosin result in uncoupling of the actin-activated ATPase activity from mechanical movement? ..