Research Topics
| James SpudichSummaryAffiliation: Stanford University Country: USA Publications
Research Grants
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Detail Information
Publications
Myosin VI walks hand-over-hand along actinZeynep Okten
Stanford University School of Medicine, Beckman Center, 279 Campus Drive, Stanford, California 94305-5307, USA
Nat Struct Mol Biol 11:884-7. 2004..The most likely explanation for this result is a hand-over-hand walking mechanism. We hypothesize the existence of a flexible element that would allow the motor to bridge the observed 72-nm distance...
Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rodDaniel Hostetter
Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA
PLoS Biol 2:e356. 2004..Truncation analysis of AD-Cterm tail fragments shows that assembly is delicately balanced, resulting in assembled myosin-II molecules that are poised to disassemble due to the phosphorylation of only three threonines...- Molecular motors: forty years of interdisciplinary researchJames A Spudich
Stanford University, Stanford, CA 94305, USA
Mol Biol Cell 22:3936-9. 2011..Molecular motor research is now at an exciting threshold of being able to enter into the realm of clinical applications... 
Molecular motors: a surprising twist in myosin VI translocationJames A Spudich
Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305, USA
Curr Biol 18:R68-70. 2008..A recent study has revealed an unexpected change in conformation of the myosin VI converter domain, essential for twisting the lever arm through a approximately 180 degrees rotation to achieve a large step along actin...- Molecular motors take tension in strideJames A Spudich
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
Cell 126:242-4. 2006..The single motor studies on dynein by Reck-Peterson et al (2006) described in this issue pave the way to understand molecular mechanisms used by this unique machine... - Myosin VI: an innovative motor that challenged the swinging lever arm hypothesisJames A Spudich
Department of Biochemistry, B400 Beckman Center, Stanford University School of Medicine, Stanford, California 94305 5307, USA
Nat Rev Mol Cell Biol 11:128-37. 2010..We now know that myosin VI does indeed operate by an unusual approximately 180 degrees lever arm swing and achieves its large step size using special structural features in its tail domain... - Differential localization in cells of myosin II heavy chain kinases during cytokinesis and polarized migrationWenchuan Liang
Department of Biochemistry, Stanford University School of Medicine, CA 94305 5307, USA
BMC Cell Biol 3:19. 2002..In this report we evaluate the biochemical properties of MHCK-C, and using fluorescence microscopy in living cells we examine the localization of GFP-labeled MHCK-A, -B, and -C in relation to GFP-myosin-II localization... - Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensingAlexander R Dunn
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 107:7746-50. 2010..This mechanism likely increases both the motor's processivity and its ability to act as an anchor under physiological conditions... - Coupled myosin VI motors facilitate unidirectional movement on an F-actin networkSivaraj Sivaramakrishnan
Department of Biochemistry, Stanford University, Stanford, CA 94305, USA
J Cell Biol 187:53-60. 2009..Furthermore, several molecules of monomeric myosin VI, which are nonprocessive in single molecule assays, can coordinate to transport cargo with similar speeds as dimers... - The mechanism of myosin VI translocation and its load-induced anchoringDavid Altman
Department of Physics, Stanford University, Stanford, CA 94305, USA
Cell 116:737-49. 2004..From our results, we propose a mechanism of myosin VI stepping that predicts a regulation through load of the motor's roles as transporter and anchor... - Long single alpha-helical tail domains bridge the gap between structure and function of myosin VIBenjamin J Spink
Department of Biochemistry, Stanford University, 279 Campus Drive, Stanford, California 94305, USA
Nat Struct Mol Biol 15:591-7. 2008..Furthermore, the cargo binding domain seems to be folded back in the presence of the catalytic head, constituting a potential regulatory mechanism that inhibits dimerization... - Engineered myosin VI motors reveal minimal structural determinants of directionality and processivityJung Chi Liao
Department of Bioengineering, Stanford University, Stanford, CA 94305, USA
J Mol Biol 392:862-7. 2009..Further, we observed robust processive stepping of motors with artificial lever arms, demonstrating that processivity can arise without optimizing lever arm composition or mechanics... - Identification of a minimal myosin Va binding site within an intrinsically unstructured domain of melanophilinNathan C Geething
Department of Biochemistry, Stanford University, Stanford, California 94041, USA
J Biol Chem 282:21518-28. 2007..We discuss the possible implications of protein intrinsic disorder in recruitment and maintenance of myosin Va on melanosome membranes... - The power stroke of myosin VI and the basis of reverse directionalityZev Bryant
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
Proc Natl Acad Sci U S A 104:772-7. 2007..We suggest that the lever arm rotates approximately 180 degrees between pre- and poststroke conformations... - Role of the lever arm in the processive stepping of myosin VThomas J Purcell
Department of Biochemistry, Stanford University Medical Center, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 99:14159-64. 2002..These data show that for myosin V with its normal proximal tail domain, both heads and a long lever arm are required for large, processive steps... - Precise positioning of myosin VI on endocytic vesicles in vivoDavid Altman
Department of Biochemistry, Stanford University Medical Center, Stanford, California, United States of America
PLoS Biol 5:e210. 2007..Our results are therefore consistent with vesicle-associated myosin VI existing as a processive dimer, capable of its known trafficking function... - Quantitation of the distribution and flux of myosin-II during cytokinesisDouglas N Robinson
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
BMC Cell Biol 3:4. 2002..To achieve this goal, the distribution and flux of the cell division machinery must be quantified. Here we report the first quantitative analysis of the distribution and flux of myosin-II, an essential element of the contractile ring... - Dynacortin is a novel actin bundling protein that localizes to dynamic actin structuresDouglas N Robinson
Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
J Biol Chem 277:9088-95. 2002..These results indicate that cells have evolved different actin-filament cross-linking proteins with complementary cellular distributions that collaborate to orchestrate complex cell shape changes... - A flexible domain is essential for the large step size and processivity of myosin VIRonald S Rock
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
Mol Cell 17:603-9. 2005..Based on these results, we present a mechanical model that describes stepping under an applied load... - Principles of unconventional myosin function and targetingM Amanda Hartman
Department of Biochemistry, Stanford University, Stanford, California 94305, USA
Annu Rev Cell Dev Biol 27:133-55. 2011.... - Structure of an F-actin trimer disrupted by gelsolin and implications for the mechanism of severingJohn F Dawson
Department of Biochemistry, Stanford University School of Medicine, California 94305, USA
J Biol Chem 278:1229-38. 2003..We also show that segment-1 of gelsolin is able to sever actin filaments, although the severing activity of segment-1 is significantly lower than full-length gelsolin... - Dynamic charge interactions create surprising rigidity in the ER/K alpha-helical protein motifSivaraj Sivaramakrishnan
Departments of Biochemistry, Applied Physics, and Physics, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 105:13356-61. 2008..We propose that the significant rigidity of the ER/K alpha-helix can help regulate protein function, as a force transducer between protein subdomains... - A mitotic kinesin-like protein required for normal karyokinesis, myosin localization to the furrow, and cytokinesis in DictyosteliumGandikota S Lakshmikanth
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 101:16519-24. 2004..Given these results, we hypothesize a mechanism for myosin II translocation to the furrow to set up the contractile ring... - Building and using optical traps to study properties of molecular motorsSarah E Rice
Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305, USA
Methods Enzymol 361:112-33. 2003 - Predicting allosteric communication in myosin via a pathway of conserved residuesSusan Tang
Department of Bioengineering, Stanford University, Stanford, CA 94305, USA
J Mol Biol 373:1361-73. 2007..We provide a refined, downloadable application and source code (on https://simtk.org) to share this tool with the wider community (https://simtk.org/home/allopathfinder)... - Extending the absorbing boundary method to fit dwell-time distributions of molecular motors with complex kinetic pathwaysJung Chi Liao
Department of Bioengineering, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 104:3171-6. 2007.... - Dynamics of the unbound head during myosin V processive translocationAlexander R Dunn
Stanford University School of Medicine, Beckman Center, 279 Campus Drive, Stanford, California 94305 5307, USA
Nat Struct Mol Biol 14:246-8. 2007..We find that the unbound head rotates freely about the lever-arm junction, a trait that likely facilitates travel through crowded actin meshworks... - Two important polymers cross pathsJames A Spudich
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307, USA
Proc Natl Acad Sci U S A 101:15825-6. 2004 - The localization of inner centromeric protein (INCENP) at the cleavage furrow is dependent on Kif12 and involves interactions of the N terminus of INCENP with the actin cytoskeletonQian Chen
Section of Molecular Cell and Developmental Biology, University of Texas at Austin, Austin, TX 78712, USA
Mol Biol Cell 18:3366-74. 2007..Once INCENP reaches the equatorial cortex, it associates with the actin cytoskeleton where it then concentrates toward the end of cytokinesis... - Mechanics and regulation of cytokinesisDouglas N Robinson
Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N. Wolfe St, Baltimore, MD 21205, USA
Curr Opin Cell Biol 16:182-8. 2004..A genuine quantitative understanding of cytokinesis must include a thorough analysis of the mechanical properties of the cortex and how signals modify these properties to dictate a well-controlled, error-free cytokinesis... - How does ATP hydrolysis control actin's associations?Elena P Sablin
Department of Biochemistry Biophysics, University of California, San Francisco, CA 94143, USA
Proc Natl Acad Sci U S A 99:10945-7. 2002 - A crossbridge too farJames A Spudich
Nat Cell Biol 4:E8-10. 2002
Research Grants
- MYOSIN MOVEMENT IN VITRO-MOLECULAR CHARACTERIZATIONJames A Spudich; Fiscal Year: 2010..Understanding how they work is therefore fundamental to understanding both their normal and pathologic behaviors. ..
- CYTOKINESIS IN DICTYOSTELIUM--A MOLECULAR ANALYSISJames Spudich; Fiscal Year: 2001..Finally, synchronization of cytokinesis in populations of Dictyostelium cells for biochemical studies will be attempted. ..
- HOMOLOGOUS RECOMBINATION IN DICTYOSTELIUMJames Spudich; Fiscal Year: 1992....
- MYOSIN MOVEMENT IN VITRO-MOLECULAR CHARACTERIZATIONJames Spudich; Fiscal Year: 2003..myosin mutants altered in lever arm length? 6) How does a single point mutation near the nucleotide binding site of the motor domain of myosin result in uncoupling of the actin-activated ATPase activity from mechanical movement? ..
- Myosin Movement in Vitro - Molecular CharacterizationJames Spudich; Fiscal Year: 2007..We will use multiple approaches to reveal regions that become exposed during its chemomechanical cycle. ..
- Myosin-II Dynamics and CytokinesisJames Spudich; Fiscal Year: 2005..This method will allow dual visualization of myosin-II and other fluorescent-labeled cytokinesis-related proteins. ..
- MYOSIN MOVEMENT IN VITRO--MOLECULAR CHARACTERIZATIONJames Spudich; Fiscal Year: 1999..How can we improve the feedback-enhanced laser trap system? 5. How will we use the improved feedback-enhanced laser trap system to study myosin function? ..
- MYOSIN MOVEMENT IN VITRO--MOLECULAR CHARACTERIZATIONJames Spudich; Fiscal Year: 1993..Such correlations of ATPase activity, force, and velocity should lead to a better understanding of the molecular basis of myosin movement...
- MYOSIN MOVEMENT IN VITRO: MOLECULAR CHARACTERIZATIONJames Spudich; Fiscal Year: 1991..We also propose to examine whether organelle movements in vivo involve myosin- driven translocations analogous to the myosin-coated bead movements on actin filaments in vitro...