Wen Jun Shen

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. pmc Effects of rosiglitazone and high fat diet on lipase/esterase expression in adipose tissue
    Wen Jun Shen
    Division of Endocrinology, Stanford University and VA Palo Alto Health Care System, Palo Alto, CA 94304, USA
    Biochim Biophys Acta 1771:177-84. 2007
  2. ncbi request reprint Regulation of hormone-sensitive lipase in islets
    Wen Jun Shen
    Department of Medicine, Stanford University, Stanford, CA 94305, USA
    Diabetes Res Clin Pract 75:14-26. 2007
  3. pmc Hormone-sensitive lipase modulates adipose metabolism through PPARγ
    Wen Jun Shen
    Division of Endocrinology, Stanford University, CA 94305 5103, USA
    Biochim Biophys Acta 1811:9-16. 2011
  4. ncbi request reprint Interaction of hormone-sensitive lipase with steroidogenic acute regulatory protein: facilitation of cholesterol transfer in adrenal
    Wen Jun Shen
    Veterans Affairs Palo Alto Health Care System, Palo Alto, California 94304 1290, USA
    J Biol Chem 278:43870-6. 2003
  5. pmc Regulation of expression and function of scavenger receptor class B, type I (SR-BI) by Na+/H+ exchanger regulatory factors (NHERFs)
    ZhiGang Hu
    Geriatric Research, Education and Clinical Center, Veterans Affairs Palo Alto Health Care System, Palo Alto, California 94304, USA
    J Biol Chem 288:11416-35. 2013
  6. pmc Age-related modulation of the effects of obesity on gene expression profiles of mouse bone marrow and epididymal adipocytes
    Li Fen Liu
    Division of Endocrinology, Stanford University, Stanford, California, United States of America
    PLoS ONE 8:e72367. 2013
  7. pmc Cholesterol ester droplets and steroidogenesis
    Fredric B Kraemer
    VA Palo Alto Health Care System, Palo Alto, CA 94304, USA
    Mol Cell Endocrinol 371:15-9. 2013
  8. ncbi request reprint Resistance to high-fat diet-induced obesity and altered expression of adipose-specific genes in HSL-deficient mice
    Kenji Harada
    Veterans Affairs Palo Alto Health Care System, Palo Alto, California 94304, USA
    Am J Physiol Endocrinol Metab 285:E1182-95. 2003
  9. pmc Ablation of vimentin results in defective steroidogenesis
    Wen Jun Shen
    Division of Endocrinology, Gerontology, and Metabolism, Stanford University, Palo Alto, California 94304, USA
    Endocrinology 153:3249-57. 2012
  10. pmc Lipid droplet metabolism
    Victor K Khor
    aVeterans Affairs Palo Alto Healthcare System, Palo Alto bDivision of Endocrinology, Stanford University, Stanford, California, USA
    Curr Opin Clin Nutr Metab Care 16:632-7. 2013

Collaborators

Detail Information

Publications40

  1. pmc Effects of rosiglitazone and high fat diet on lipase/esterase expression in adipose tissue
    Wen Jun Shen
    Division of Endocrinology, Stanford University and VA Palo Alto Health Care System, Palo Alto, CA 94304, USA
    Biochim Biophys Acta 1771:177-84. 2007
    ..In conclusion, these data highlight the complexity of the regulation of the expression of intracellular neutral lipase/esterases involved in lipolysis...
  2. ncbi request reprint Regulation of hormone-sensitive lipase in islets
    Wen Jun Shen
    Department of Medicine, Stanford University, Stanford, CA 94305, USA
    Diabetes Res Clin Pract 75:14-26. 2007
    ..Therefore, these results suggest that the beta-cell isoform of HSL is involved in maintaining lipid homeostasis in islets and contributes to the proper control of GSIS...
  3. pmc Hormone-sensitive lipase modulates adipose metabolism through PPARγ
    Wen Jun Shen
    Division of Endocrinology, Stanford University, CA 94305 5103, USA
    Biochim Biophys Acta 1811:9-16. 2011
    ..These results suggest that one of the mechanisms by which HSL modulates adipose metabolism is by providing intrinsic ligands or pro-ligands for PPARγ...
  4. ncbi request reprint Interaction of hormone-sensitive lipase with steroidogenic acute regulatory protein: facilitation of cholesterol transfer in adrenal
    Wen Jun Shen
    Veterans Affairs Palo Alto Health Care System, Palo Alto, California 94304 1290, USA
    J Biol Chem 278:43870-6. 2003
    ..It is proposed that the interaction of HSL with StAR in cytosol increases the hydrolytic activity of HSL and that together HSL and StAR facilitate cholesterol movement from lipid droplets to mitochondria for steroidogenesis...
  5. pmc Regulation of expression and function of scavenger receptor class B, type I (SR-BI) by Na+/H+ exchanger regulatory factors (NHERFs)
    ZhiGang Hu
    Geriatric Research, Education and Clinical Center, Veterans Affairs Palo Alto Health Care System, Palo Alto, California 94304, USA
    J Biol Chem 288:11416-35. 2013
    ..Collectively, these data establish NHERF1 and NHERF2 as SR-BI protein binding partners that play a negative role in the regulation of SR-BI expression, selective CE transport, and steroidogenesis...
  6. pmc Age-related modulation of the effects of obesity on gene expression profiles of mouse bone marrow and epididymal adipocytes
    Li Fen Liu
    Division of Endocrinology, Stanford University, Stanford, California, United States of America
    PLoS ONE 8:e72367. 2013
    ..In conclusion, obesity affects age-associated alterations in gene expression in both epididymal and bone marrow adipocytes regardless of diet or genetic background. ..
  7. pmc Cholesterol ester droplets and steroidogenesis
    Fredric B Kraemer
    VA Palo Alto Health Care System, Palo Alto, CA 94304, USA
    Mol Cell Endocrinol 371:15-9. 2013
    ..These results suggest that vimentin is important in the maintenance of CE-rich LDs and in the movement of cholesterol for steroidogenesis...
  8. ncbi request reprint Resistance to high-fat diet-induced obesity and altered expression of adipose-specific genes in HSL-deficient mice
    Kenji Harada
    Veterans Affairs Palo Alto Health Care System, Palo Alto, California 94304, USA
    Am J Physiol Endocrinol Metab 285:E1182-95. 2003
    ..Our results suggest that impaired lipolysis in HSL deficiency affects lipid metabolism through alterations of adipose differentiation and adipose-derived hormone levels...
  9. pmc Ablation of vimentin results in defective steroidogenesis
    Wen Jun Shen
    Division of Endocrinology, Gerontology, and Metabolism, Stanford University, Palo Alto, California 94304, USA
    Endocrinology 153:3249-57. 2012
    ....
  10. pmc Lipid droplet metabolism
    Victor K Khor
    aVeterans Affairs Palo Alto Healthcare System, Palo Alto bDivision of Endocrinology, Stanford University, Stanford, California, USA
    Curr Opin Clin Nutr Metab Care 16:632-7. 2013
    ..This review provides a summary of these recent publications on lipid droplet metabolism...
  11. ncbi request reprint Adrenal neutral cholesteryl ester hydrolase: identification, subcellular distribution, and sex differences
    Fredric B Kraemer
    Veterans Affairs Palo Alto Health Care System, Palo Alto, California 94304, USA
    Endocrinology 143:801-6. 2002
    ..Thus, HSL appears to be responsible for most, if not all, of adrenal neutral CEH activity, is prominently expressed in microsomes, and its activity is influenced by sex...
  12. pmc Hormone-sensitive lipase-knockout mice maintain high bone density during aging
    Wen Jun Shen
    Division of Endocrinology, Stanford University, Stanford, CA 94305 5103, USA
    FASEB J 25:2722-30. 2011
    ..5-fold, P<0.05) and osteocalcin (4-fold, P<0.05). The absence of HSL directs cells within the bone marrow toward osteoblast differentiation and favors the maintenance of bone density with aging...
  13. pmc Differential roles of cysteine residues in the cellular trafficking, dimerization, and function of the high-density lipoprotein receptor, SR-BI
    Jie Hu
    Geriatric Research, Education and Clinical Center, VA Palo Alto Health Care System, Palo Alto, California 94304, United States
    Biochemistry 50:10860-75. 2011
    ....
  14. ncbi request reprint Hormone-sensitive lipase: control of intracellular tri-(di-)acylglycerol and cholesteryl ester hydrolysis
    Fredric B Kraemer
    Division of Endocrinology, Department of Medicine, Stanford University, Stanford, CA, USA
    J Lipid Res 43:1585-94. 2002
    ..The published reports examining the regulation, and function of HSL in normal physiology and disease are reviewed in this paper...
  15. pmc Fat-specific protein 27 modulates nuclear factor of activated T cells 5 and the cellular response to stress
    Masami Ueno
    Veterans Administration Palo Alto Health Care System, Palo Alto, CA, USA
    J Lipid Res 54:734-43. 2013
    ..These results suggest that FSP27 not only modulates LD homeostasis but also modulates the response to osmotic stress via a physical interaction with NFAT5 at the LD surface...
  16. pmc MicroRNAs 125a and 455 repress lipoprotein-supported steroidogenesis by targeting scavenger receptor class B type I in steroidogenic cells
    ZhiGang Hu
    Geriatric Research, Education and Clinical Center, VA Palo Alto Health Care System, Palo Alto, California, USA
    Mol Cell Biol 32:5035-45. 2012
    ....
  17. ncbi request reprint The LDL receptor is not necessary for acute adrenal steroidogenesis in mouse adrenocortical cells
    Fredric B Kraemer
    Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA
    Am J Physiol Endocrinol Metab 292:E408-12. 2007
    ..In contrast, HSL occupies a vital role in adrenal steroidogenesis because of its link to utilization of selectively delivered cholesteryl esters from lipoproteins...
  18. pmc Functional interaction of hormone-sensitive lipase and perilipin in lipolysis
    Wen Jun Shen
    Division of Endocrinology, Stanford University, Stanford, California 94305, USA
    J Lipid Res 50:2306-13. 2009
    ..The results suggest the translocation of HSL to the lipid droplet occurs by virtue of Plin localization to the surface of lipid droplets and a physical interaction of HSL occurring with sequences within the N-terminal region of Plin...
  19. pmc Nordihydroguaiaretic acid improves metabolic dysregulation and aberrant hepatic lipid metabolism in mice by both PPARα-dependent and -independent pathways
    Haiyan Zhang
    Geriatric Research, Education and Clinical Center, VA Palo Alto Health Care System, Palo Alto, CA 94304, USA
    Am J Physiol Gastrointest Liver Physiol 304:G72-86. 2013
    ..However, PPARα-independent pathways also contribute to NDGA's action to ameliorate hepatic steatosis...
  20. pmc Characterization of age-related gene expression profiling in bone marrow and epididymal adipocytes
    Li Fen Liu
    Division of Endocrinology, Stanford University, CA 94305 5103, USA
    BMC Genomics 12:212. 2011
    ..The aim of this study was to characterize and compare the age-related gene expression profiles in bone marrow adipocytes and epididymal adipocytes...
  21. pmc Hormonal regulation of microRNA expression in steroid producing cells of the ovary, testis and adrenal gland
    ZhiGang Hu
    Geriatric Research, Education and Clinical Center, VA Palo Alto Health Care System, Palo Alto, California, United States of America Division of Endocrinology, Stanford University, Stanford, California, United States of America
    PLoS ONE 8:e78040. 2013
    ..Key observations were also confirmed using hormone (Bt2cAMP)-treated mouse Leydig tumor cells, MLTC-1, and primary rat ovarian granulosa cells...
  22. pmc Vimentin is a functional partner of hormone sensitive lipase and facilitates lipolysis
    Wen Jun Shen
    Division of Endocrinology, Stanford University and VA Palo Alto Health Care System, Palo Alto, California 94304, USA
    J Proteome Res 9:1786-94. 2010
    ..The current studies provide evidence that vimentin participates in lipolysis through direct, hormonally regulated interactions with HSL...
  23. pmc The proteome of cholesteryl-ester-enriched versus triacylglycerol-enriched lipid droplets
    Victor K Khor
    Division of Endocrinology, Gerontology and Metabolism, Stanford University, Stanford, California, United States of America Veterans Affairs Palo Alto Health Care System, Palo Alto, California, United States of America
    PLoS ONE 9:e105047. 2014
    ..This study is the first to compare the proteins found on CE-enriched LDs with TAG-enriched LDs and constitutes the first step in creating a better understanding of the proteins found on CE-enriched LDs in steroidogenic cells. ..
  24. ncbi request reprint Scavenger receptor class B type I (SR-BI): a versatile receptor with multiple functions and actions
    Wen Jun Shen
    Geriatric Research, Education and Clinical Center, VA Palo Alto Health Care System, Palo Alto, California 94304 Division of Endocrinology, Stanford University, Stanford, California 94305
    Metabolism 63:875-86. 2014
    ....
  25. ncbi request reprint Mutational analysis of the "regulatory module" of hormone-sensitive lipase
    Jining Wang
    VA Palo Alto Health Care System, Palo Alto, California, USA
    Biochemistry 44:1953-9. 2005
    ....
  26. ncbi request reprint Hormone-sensitive lipase is required for high-density lipoprotein cholesteryl ester-supported adrenal steroidogenesis
    Fredric B Kraemer
    Veterans Affairs Palo Alto Health Care System, California 94304, USA
    Mol Endocrinol 18:549-57. 2004
    ..These findings suggest that HSL is functionally linked to the selective pathway and is critically involved in the intracellular processing and availability of cholesterol for adrenal steroidogenesis...
  27. pmc p38 MAPK regulates steroidogenesis through transcriptional repression of STAR gene
    Syed Kashif Zaidi
    Geriatric ResearchEducation and Clinical Center GRECC 182B, VA Palo Alto Health Care System, 3801 Miranda Avenue, Palo Alto, California 94304, USADivision of EndocrinologyDivision of Gastroenterology and HepatologyStanford University, Stanford, California 94305, USADepartment of Obstetrics and GynecologyUniversity of South Florida College of Medicine, Tampa, Florida 33612, USAState Key Laboratory of Cellular Stress BiologySchool of Life Sciences, Xiamen University, Xiamen, Fujian 361005, ChinaProgram in Molecular MedicineUniversity of Massachusetts Medical School, Worcester, Massachusetts 01605, USAGeriatric ResearchEducation and Clinical Center GRECC 182B, VA Palo Alto Health Care System, 3801 Miranda Avenue, Palo Alto, California 94304, USADivision of EndocrinologyDivision of Gastroenterology and HepatologyStanford University, Stanford, California 94305, USADepartment of Obstetrics and GynecologyUniversity of South Florida College of Medicine, Tampa, Florida 33612, USAState Key Laboratory of Cellular Stress BiologySchool of Life Sciences, Xiamen University, Xiamen, Fujian 361005, ChinaProgram in Molecular MedicineUniversity of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Mol Endocrinol 53:1-16. 2014
    ..Collectively, these data provide novel mechanistic information about how oxidant-sensitive p38 MAPKs, particularly p38 MAPKα, contribute to the negative regulation of Star gene expression and inhibit steroidogenesis. ..
  28. doi request reprint Adipocytes decrease Runx2 expression in osteoblastic cells: roles of PPARγ and adiponectin
    Li Fen Liu
    Division of Endocrinology, Stanford University, Stanford, California, USA
    J Cell Physiol 225:837-45. 2010
    ..Thus, we conclude that adipocytes modulate key metabolic functions of osteoblasts through the release of secretory products. PPARγ plays a key role in mediating the effects of adipocytes on osteoblasts...
  29. pmc LDL and cAMP cooperate to regulate the functional expression of the LRP in rat ovarian granulosa cells
    Salman Azhar
    Geriatric Research, Education, and Clinical Center, Department of Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA
    J Lipid Res 47:2538-50. 2006
    ..In summary, our studies demonstrate a role for LRP in lipoprotein-supported ovarian granulosa cell steroidogenesis...
  30. pmc IL-17 regulates adipogenesis, glucose homeostasis, and obesity
    Luis A Zuniga
    Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305 5324, USA
    J Immunol 185:6947-59. 2010
    ..Our findings implicate IL-17 as a negative regulator of adipogenesis and glucose metabolism in mice, and show that it delays the development of obesity...
  31. pmc Cellular cholesterol delivery, intracellular processing and utilization for biosynthesis of steroid hormones
    Jie Hu
    Geriatric Research, Education and Clinical Center, VA Palo Alto Health Care System, Palo Alto, California 94304, USA
    Nutr Metab (Lond) 7:47. 2010
    ....
  32. ncbi request reprint Generation of novel adipocyte monolayer cultures from embryonic stem cells
    Theresa L Chen
    Veterans Affairs, Palo Alto Health Care System, Palo Alto, CA 94304, USA
    Stem Cells Dev 16:371-80. 2007
    ..The adipocytes are functionally active, as evidenced by their response to lipolytic agents, such as forskolin, Bt2-cAMP, and isoproterenol, with more than 20-fold increases in glycerol release...
  33. doi request reprint Oxidative stress-induced inhibition of adrenal steroidogenesis requires participation of p38 mitogen-activated protein kinase signaling pathway
    Parveen Abidi
    Department of Veterans Affairs Palo Alto Health Care System, Geriatric Research, Education and Clinical Center, 3801 Miranda Avenue, Palo Alto, California 94304, USA
    J Endocrinol 198:193-207. 2008
    ..These results indicate that activated p38 MAPK mediates oxidant (excessive oxidative stress)-induced inhibition of adrenal steroidogenesis...
  34. ncbi request reprint Overexpression of leptin in transgenic mice leads to decreased basal lipolysis, PKA activity, and perilipin levels
    Yaohuang Ke
    Department of Laboratory Medicine, 505 Parnassus Avenue, University of California, San Francisco, CA 94143 0134, USA
    Biochem Biophys Res Commun 312:1165-70. 2003
    ....
  35. ncbi request reprint Fatty acid-binding protein-hormone-sensitive lipase interaction. Fatty acid dependence on binding
    Anne E Jenkins-Kruchten
    Department of Biochemistry, The University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 278:47636-43. 2003
    ..These results suggest that binding and activation of HSL by FABPs are separate and distinct functions and that HSL contains a site for fatty acid binding that allows for FABP association...
  36. ncbi request reprint Control of adipose triglyceride lipase action by serine 517 of perilipin A globally regulates protein kinase A-stimulated lipolysis in adipocytes
    Hideaki Miyoshi
    Jean Mayer United States Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts 02111, USA
    J Biol Chem 282:996-1002. 2007
    ..The contributions of other PKA sites to PKA-stimulated lipolysis are manifested only in the presence of phosphorylated or phosphomimetic serine 517. Thus, serine 517 is a novel "master regulator" of PKA-stimulated adipocyte lipolysis...
  37. ncbi request reprint Cardiac overexpression of hormone-sensitive lipase inhibits myocardial steatosis and fibrosis in streptozotocin diabetic mice
    Masami Ueno
    Third Department of Internal Medicine, Faculty of Medical Science, University of Fukui, Fukui, Japan
    Am J Physiol Endocrinol Metab 294:E1109-18. 2008
    ..In conclusion, since HSL overexpression inhibits cardiac steatosis and fibrosis by apparently hydrolyzing toxic lipid metabolites, cardiac HSL could be a therapeutic target for regulating diabetic cardiomyopathy...
  38. ncbi request reprint Absence of hormone-sensitive lipase inhibits obesity and adipogenesis in Lep ob/ob mice
    Motohiro Sekiya
    Departments of Metabolic Diseases and Cardiovascular Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113 8655, Japan
    J Biol Chem 279:15084-90. 2004
    ..Thus, deficiency of both leptin and HSL has unmasked novel roles of HSL in adipogenesis as well as in feeding behavior...
  39. ncbi request reprint Function of hormone-sensitive lipase in diacylglycerol-protein kinase C pathway
    Hideo Kanehara
    Third Department of Internal Medicine, Faculty of Medical Science, University of Fukui, Fukui 910 1193, Japan
    Diabetes Res Clin Pract 65:209-15. 2004
    ....
  40. ncbi request reprint Cardiac gene expression profile and lipid accumulation in response to starvation
    Jinya Suzuki
    Third Department of Internal Medicine, Fukui Medical University, Fukui 910 1193, Japan
    Am J Physiol Endocrinol Metab 283:E94-E102. 2002
    ..These data provide a broad perspective of the molecular events occurring physiologically in the heart in response to starvation...