Research Topics
Species | Nader PourmandSummaryAffiliation: Stanford University Country: USA Publications
| Collaborators
|
Detail Information
Publications
Direct electrical detection of DNA synthesisNader Pourmand
Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA
Proc Natl Acad Sci U S A 103:6466-70. 2006..Based on current understanding of polarizable interfaces, we propose that the electrode detects proton removal from the 3'-hydroxyl group of the DNA molecule during phosphodiester bond formation...
Branch migration displacement assay with automated heuristic analysis for discrete DNA length measurement using DNA microarraysNader Pourmand
Stanford Genome Technology Center, Palo Alto, CA 94304, USA
Proc Natl Acad Sci U S A 104:6146-51. 2007..Our technique will facilitate the rapid deduction of identity, length, and number of repeats for the multiple STRs in an unknown DNA sample...
Rapid and highly informative diagnostic assay for H5N1 influenza virusesNader Pourmand
Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America
PLoS ONE 1:e95. 2006..Knowledge of the predicted functional sequences of the HA will enhance H5N1 avian influenza surveillance efforts...
Suspension bead array branch migration displacement assay for rapid STR analysisAndrea Villablanca
Stanford Genome Technology Center, Stanford University, Palo Alto, CA, USA
Electrophoresis 29:4109-14. 2008..The multiplex, bead-based approach provides a high-throughput and more portable STR analysis...
PathogenMip assay: a multiplex pathogen detection assayMichael S Akhras
Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America
PLoS ONE 2:e223. 2007....
Sentinel-base DNA genotyping using multiple sequencing primers for high-risk human papillomavirusesBaback Gharizadeh
Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto, CA 94304, USA
Mol Cell Probes 20:230-8. 2006..These proof-of-concept studies establish the assay to be accurate, reliable, rapid, flexible, and cost-effective, providing evidence of the feasibility this technique for use in clinical settings...
Sensitive giant magnetoresistive-based immunoassay for multiplex mycotoxin detectionAndy C Mak
Stanford Genome Technology Center, Stanford University, 855 California Ave, Palo Alto, CA 94304, USA
Biosens Bioelectron 25:1635-9. 2010..Here we demonstrate the simultaneous detection of multiple mycotoxins (aflatoxins B(1), zearalenone and HT-2) and show that a detection limit of 50 pg/mL can be achieved...
PathogenMIPer: a tool for the design of molecular inversion probes to detect multiple pathogensSreedevi Thiyagarajan
Stanford University, Palo Alto, CA, USA
BMC Bioinformatics 7:500. 2006..The system can harness the genetic variability available in an entire genome in designing specific probes for the detection of multiple co-infections in a single tube using a MIP assay...
Current rectification with poly-l-lysine-coated quartz nanopipettesSenkei Umehara
Stanford Genome Technology Center, Department of Biochemistry, Stanford University School of Medicine, Stanford University, 855 California Avenue, Palo Alto, California 94304, USA
Nano Lett 6:2486-92. 2006..This surface condition dependence can be used as the fundamental principle of multi-purpose real-time in vivo biosensors...
Detection of point mutations associated with antibiotic resistance in Pseudomonas aeruginosaNeda Gorgani
Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA
Int J Antimicrob Agents 34:414-8. 2009..Results of this study suggest Pyrosequencing as a substitute for traditional methods as it provides a rapid and reliable technique for determining the antibiotic resistance pattern of a given bacterial strain in <1 h...
Determination of hepatitis C virus genotype by PyrosequencingElahe Elahi
Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto, CA 94304, USA
J Virol Methods 109:171-6. 2003..For rapid population-specific HCV subtyping, a multiplex assay was developed. This study demonstrates the suitability of this technology for low-cost, high throughput and accurate microbial genotyping...
Single DNA molecule detection using nanopipettes and nanoparticlesMiloslav Karhanek
Department of Biochemistry, Stanford Genome Technology Center, Stanford University, Stanford, CA 94304, USA
Nano Lett 5:403-7. 2005....
Multiplex protein assays based on real-time magnetic nanotag sensingSebastian J Osterfeld
Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 105:20637-40. 2008..The multianalyte ability, sensitivity, scalability, and ease of use of the MNT-based protein assay technology make it a strong contender for versatile and portable molecular diagnostics in both research and clinical settings...
Giant magnetoresistive biochip for DNA detection and HPV genotypingLiang Xu
Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305 4045, USA
Biosens Bioelectron 24:99-103. 2008..This is the first demonstration of magnetic DNA detection using plasmid-derived samples. This study provides a direct proof that GMR sensors can be used for biomedical applications...
Methodological improvements of pyrosequencing technologyBaback Gharizadeh
Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto, CA 94304, USA
J Biotechnol 124:504-11. 2006..In this study, these issues have been addressed to increase signal quality and assure sequence accuracy...
Connector inversion probe technology: a powerful one-primer multiplex DNA amplification system for numerous scientific applicationsMichael S Akhras
Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America
PLoS ONE 2:e915. 2007..For the readers' convenience, we also provide an on-line tutorial with user-interface software application CIP creator 1.0.1, for custom probe generation from virtually any new or established primer-pairs...
Genotyping African haplotypes in ATM using a co-spotted single-base extension assayManeesh Jain
Stanford Genome Technology Center, Palo Alto, California 94304, USA
Hum Mutat 22:214-21. 2003..Arrayed SBE was a robust tool for this analysis that could be applied to any situation requiring the genotyping of a few SNPs in many individuals...
Simultaneous measurement of nonlinearity and electrochemical impedance for protein sensing using two-tone excitationJonathan S Daniels
Stanford Genome Technology Center, Stanford University Dept of Biochemistry, USA
Conf Proc IEEE Eng Med Biol Soc 2008:5753-6. 2008..We demonstrate that changes in nonlinearity can discriminate protein binding. Our measurements suggest that target binding can alter nonlinearity via the voltage dependence of the ionic double layer...
The relationship between human papillomavirus status and other molecular prognostic markers in head and neck squamous cell carcinomasChristina S Kong
Department of Pathology, Stanford University, Santa Cruz, CA, USA
Int J Radiat Oncol Biol Phys 74:553-61. 2009....
Large-scale pyrosequencing of synthetic DNA: a comparison with results from Sanger dideoxy sequencingBaback Gharizadeh
Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto, CA 94304, USA
Electrophoresis 27:3042-7. 2006..Although more automation and quality control measures are needed, Pyrosequencing was shown to be a fast and convenient method for determining short stretches of DNA sequence...
Type-specific multiple sequencing primers: a novel strategy for reliable and rapid genotyping of human papillomaviruses by pyrosequencing technologyBaback Gharizadeh
Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA
J Mol Diagn 7:198-205. 2005..The multiple sequencing primer method has proved to be a multifaceted approach for typing of human papillomaviruses by DNA sequencing technologies...
Detection of gyrA mutations associated with ciprofloxacin resistance in Neisseria gonorrhoeae by rapid and reliable pre-programmed short DNA sequencingBaback Gharizadeh
Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto, CA 94304, USA
Int J Antimicrob Agents 26:486-90. 2005..The new method used in the present study has the potential for rapid and reliable identification of known as well as previously unknown drug resistance mutations...
p16(INK4A) immunohistochemical staining may be helpful in distinguishing branchial cleft cysts from cystic squamous cell carcinomas originating in the oropharynxReetesh K Pai
Department of Pathology, Stanford University, Stanford, CA 94305, USA
Cancer 117:108-19. 2009..We investigated p16(INK4A) expression in branchial cleft cysts and its utility in distinguishing branchial cleft cysts from metastatic head and neck squamous cell carcinomas (SCCs) in fine-needle aspiration biopsies (FNABs)...
Bioluminescence regenerative cycle (BRC) system: theoretical considerations for nucleic acid quantification assaysArjang Hassibi
Center for Integrated Systems, Stanford University, Stanford, CA, USA
Biophys Chem 116:175-85. 2005..Sensitivity is not constrained by detector performance but rather by background bioluminescence caused by contamination by either PPi or ATP (adenosine triphosphate)...
Pyrosequencing: an accurate detection platform for single nucleotide polymorphismsHossein Fakhrai-Rad
Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA
Hum Mutat 19:479-85. 2002..In addition, we describe new schemes for template preparation and multiplexing as an effort for cost reduction in large-scale studies...
Fisher's theorems for multivariable, time- and space-dependent systems, with applications in population genetics and chemical kineticsMarcel O Vlad
Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
Proc Natl Acad Sci U S A 102:9848-53. 2005....
Molecular probe technology detects bacteria without cultureRichard W Hyman
Departments of Biochemistry, Stanford University, Stanford, CA, USA
BMC Microbiol 12:29. 2012..In this communication, we report the first results of employing our molecular probes to detect bacteria in clinical samples...
Multiplex PyrosequencingNader Pourmand
Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto, CA 94304, USA
Nucleic Acids Res 30:e31. 2002..Here, we demonstrate the use of this multiplex Pyrosequencing for single nucleotide polymorphisms genotyping and microbial typing...
DNA-functionalized MFe2O4 (M = Fe, Co, or Mn) nanoparticles and their hybridization to DNA-functionalized surfacesDavid B Robinson
IBM T. J. Watson Research Center, Yorktown Heights, New York 10598, USA
Langmuir 21:3096-103. 2005..These particles may find valuable applications involving the magnetic detection of small numbers of biomolecules using spin valves, magnetic tunnel junctions, or other sensors...
Patterns of known and novel small RNAs in human cervical cancerWeng Onn Lui
Department of Pathology, Stanford University School of Medicine, Stanford, California 94305 5324, USA
Cancer Res 67:6031-43. 2007..In addition to the known miRNAs, we found a number of novel miRNAs and an additional set of small RNAs that do not meet miRNA criteria...
Characterization of synthetic DNA bar codes in Saccharomyces cerevisiae gene-deletion strainsRobert G Eason
Yale U, New Haven, CT
Proc Natl Acad Sci U S A 101:11046-51. 2004..The robust performance of the yeast gene-deletion dual oligonucleotide bar-code design in array hybridization validates the use of molecular bar codes in living cells for tracking their growth phenotype...
