S R Pfeffer

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. ncbi request reprint Unsolved mysteries in membrane traffic
    Suzanne R Pfeffer
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    Annu Rev Biochem 76:629-45. 2007
  2. pmc Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells
    Pierre Barbero
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Biol 156:511-8. 2002
  3. pmc A novel Rab9 effector required for endosome-to-TGN transport
    E Diaz
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    J Cell Biol 138:283-90. 1997
  4. ncbi request reprint Membrane transport: retromer to the rescue
    S R Pfeffer
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    Curr Biol 11:R109-11. 2001
  5. pmc Constructing a Golgi complex
    S R Pfeffer
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Biol 155:873-5. 2001
  6. ncbi request reprint Rab GTPases: specifying and deciphering organelle identity and function
    S R Pfeffer
    Dept of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Trends Cell Biol 11:487-91. 2001
  7. ncbi request reprint Biochemical characterization of mapmodulin, a protein that binds microtubule-associated proteins
    N Ulitzur
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    J Biol Chem 272:30577-82. 1997
  8. ncbi request reprint Role of Rab9 GTPase in facilitating receptor recruitment by TIP47
    K S Carroll
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Science 292:1373-6. 2001
  9. ncbi request reprint Quantitative analysis of TIP47-receptor cytoplasmic domain interactions: implications for endosome-to-trans Golgi network trafficking
    J P Krise
    Department of Biochemistry, Stanford University School of Medicine, CA 94305 5307, USA
    J Biol Chem 275:25188-93. 2000
  10. pmc Recognition of the 300-kDa mannose 6-phosphate receptor cytoplasmic domain by 47-kDa tail-interacting protein
    J G Orsel
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Proc Natl Acad Sci U S A 97:9047-51. 2000

Collaborators

  • E Diaz
  • AXEL BRUNGER
  • Jennifer J Kohler
  • Francis Barr
  • Ian G Ganley
  • Alondra Schweizer Burguete
  • Garret L Hayes
  • Ryan M Nottingham
  • Jonathan V Reddy
  • Ulf Sivars
  • Eric J Espinosa
  • Peter L Lee
  • Khambhampaty Sridevi
  • Ella H Sklan
  • P Barbero
  • F Schimmöller
  • J G Orsel
  • J P Krise
  • N Ulitzur
  • Paul M Sincock
  • John Hanna
  • Pierre Barbero
  • K S Carroll
  • P M Sincock
  • C Rancaño
  • Alexander K Haas
  • Monica Calero
  • Frank C Brown
  • I Simon
  • Eric Espinosa
  • Timothy D Fenn
  • Shirit Einav
  • Ramon L Serrano
  • Jeffrey S Glenn
  • David G Lambright
  • T Soldati
  • C Itin
  • Pehr B Harbury
  • Li Ding
  • Sven Diederichs
  • Jeffrey P Krise
  • Brian O'Connor
  • Dikran Aivazian
  • Lenka Bittova
  • Kate Carroll
  • J Krise
  • E Buell
  • S Zulley
  • J Hanna
  • A D Shapiro
  • S M Dintzis
  • B Muhlbauer
  • M Humbert
  • Y Nakajima
  • H Geissler
  • V E Velculescu
  • M A Riederer

Detail Information

Publications40

  1. ncbi request reprint Unsolved mysteries in membrane traffic
    Suzanne R Pfeffer
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    Annu Rev Biochem 76:629-45. 2007
    ..But much remains to be learned. This review highlights underlying themes in membrane traffic to help us refocus and solve many remaining and newly emerging issues that are fundamental to mammalian cell biology and human physiology...
  2. pmc Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells
    Pierre Barbero
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Biol 156:511-8. 2002
    ..Our data suggest that Rab9 remains vesicle associated until docking with the Golgi complex and is rapidly removed concomitant with or just after membrane fusion...
  3. pmc A novel Rab9 effector required for endosome-to-TGN transport
    E Diaz
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    J Cell Biol 138:283-90. 1997
    ..These data are consistent with a model in which p40 and Rab9 act together to drive the process of transport vesicle docking...
  4. ncbi request reprint Membrane transport: retromer to the rescue
    S R Pfeffer
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    Curr Biol 11:R109-11. 2001
    ..Orthologs of these proteins in mammalian cells are likely to play a similar role but their cargoes are yet to be identified...
  5. pmc Constructing a Golgi complex
    S R Pfeffer
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Biol 155:873-5. 2001
    ..These findings suggest that Golgin-45 serves as a linchpin for the maintenance of Golgi complex structure, and offer hints as to the mechanisms by which the polarized Golgi complex is constructed...
  6. ncbi request reprint Rab GTPases: specifying and deciphering organelle identity and function
    S R Pfeffer
    Dept of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Trends Cell Biol 11:487-91. 2001
    ..Rabs organize distinct protein scaffolds within a single organelle and act in a combinatorial manner with their effectors to regulate all stages of membrane traffic...
  7. ncbi request reprint Biochemical characterization of mapmodulin, a protein that binds microtubule-associated proteins
    N Ulitzur
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    J Biol Chem 272:30577-82. 1997
    ..Finally, despite identity in polypeptide sequence with a protein reported to act as an inhibitor of protein phosphatase 2A, native mapmodulin was not able to inhibit protein phosphatase 2A in Chinese hamster ovary cell cytosol...
  8. ncbi request reprint Role of Rab9 GTPase in facilitating receptor recruitment by TIP47
    K S Carroll
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Science 292:1373-6. 2001
    ..Thus, a cytosolic cargo selection device may be selectively recruited onto a specific organelle, and vesicle budding might be coupled to the presence of an active Rab GTPase...
  9. ncbi request reprint Quantitative analysis of TIP47-receptor cytoplasmic domain interactions: implications for endosome-to-trans Golgi network trafficking
    J P Krise
    Department of Biochemistry, Stanford University School of Medicine, CA 94305 5307, USA
    J Biol Chem 275:25188-93. 2000
    ..Moreover, our data suggest unexpectedly that furin, TGN38, and carboxypeptidase D may use a distinct vesicular carrier and perhaps a distinct route for transport between endosomes and the trans Golgi network...
  10. pmc Recognition of the 300-kDa mannose 6-phosphate receptor cytoplasmic domain by 47-kDa tail-interacting protein
    J G Orsel
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Proc Natl Acad Sci U S A 97:9047-51. 2000
    ..Although crystallography will be needed to define the precise interaction interface, these data provide an initial structural basis for TIP47-CI-MPR association...
  11. ncbi request reprint TIP47: a cargo selection device for mannose 6-phosphate receptor trafficking
    E Diaz
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307, USA
    Cell 93:433-43. 1998
    ..These data suggest that TIP47 binds MPR cytoplasmic domains and facilitates their collection into transport vesicles destined for the Golgi...
  12. pmc Mapmodulin: a possible modulator of the interaction of microtubule-associated proteins with microtubules
    N Ulitzur
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Proc Natl Acad Sci U S A 94:5084-9. 1997
    ..To our knowledge, mapmodulin represents the first example of a protein that can bind and potentially regulate multiple MAP proteins...
  13. ncbi request reprint A novel assay reveals a role for soluble N-ethylmaleimide-sensitive fusion attachment protein in mannose 6-phosphate receptor transport from endosomes to the trans Golgi network
    C Itin
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    J Biol Chem 272:27737-44. 1997
    ..Together with alpha-SNAP, NSF can increase the extent of transport. These data show that alpha-SNAP, a soluble component of the SNAP receptor machinery, facilitates transport from endosomes to the trans Golgi network...
  14. ncbi request reprint Quantitative analysis of the interactions between prenyl Rab9, GDP dissociation inhibitor-alpha, and guanine nucleotides
    A D Shapiro
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307, USA
    J Biol Chem 270:11085-90. 1995
    ..Finally, a previously uncharacterized minor form of GDI-alpha inhibited GDP dissociation from prenyl Rab9 by 1.9-fold and bound prenyl Rab9 with a KD of 67 nM in 0.1% Lubrol...
  15. ncbi request reprint Characterization of a 76 kDa endosomal, multispanning membrane protein that is highly conserved throughout evolution
    F Schimmöller
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Gene 216:311-8. 1998
    ..The structural features of p76 suggest that it may function as a channel or small molecule transporter in intracellular compartments throughout phylogeny. 1998 Elsevier Science B.V...
  16. pmc Cholesterol accumulation sequesters Rab9 and disrupts late endosome function in NPC1-deficient cells
    Ian G Ganley
    Department of Biochemistry, Stanford University School of Medicine, 279 Campus Drive, Stanford, CA 94305 5307, USA
    J Biol Chem 281:17890-9. 2006
    ..We conclude that cholesterol contributes directly to the sequestration of Rab9 on Niemann-Pick type C cell membranes, which in turn, disrupts mannose 6-phosphate receptor trafficking...
  17. ncbi request reprint TIP47 is not a component of lipid droplets
    P Barbero
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 95307, USA
    J Biol Chem 276:24348-51. 2001
    ..Using antibodies specific for residues 152-434 of TIP47, we show that TIP47 is not a constituent of lipid droplets...
  18. ncbi request reprint Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor
    S M Dintzis
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307
    J Biol Chem 269:12159-66. 1994
    ..These experiments suggest that the presence of the mannose 6-phosphate receptor extracellular domain may interfere with the rapid recycling of receptors from early endosomes to the cell surface and detain receptors within endosomes...
  19. ncbi request reprint Rab7 and Rab9 are recruited onto late endosomes by biochemically distinguishable processes
    T Soldati
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307, USA
    J Biol Chem 270:25541-8. 1995
    ..Since Rab7 and Rab9 are both localized at least in large part, to late endosomes, this suggests that a single organelle may bear multiple Rab recruitment machines...
  20. pmc Rab GDI: a solubilizing and recycling factor for rab9 protein
    T Soldati
    Department of Biochemistry, Stanford University School of Medicine, California 94028
    Mol Biol Cell 4:425-34. 1993
    ..Thus GDI proteins have the potential to regulate the availability of specific intracellular transport factors...
  21. pmc A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells
    Ian G Ganley
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Biol 180:159-72. 2008
    ..Importantly, our data provide a clear molecular distinction between the transport of MPRs and TGN46 to the trans-Golgi...
  22. ncbi request reprint Rab GTPases, directors of vesicle docking
    F Schimmöller
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    J Biol Chem 273:22161-4. 1998
  23. pmc Rab and Arl GTPase family members cooperate in the localization of the golgin GCC185
    Alondra Schweizer Burguete
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 132:286-98. 2008
    ....
  24. pmc Multiple Rab GTPase binding sites in GCC185 suggest a model for vesicle tethering at the trans-Golgi
    Garret L Hayes
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Mol Biol Cell 20:209-17. 2009
    ..Golgi-Rab binding sites may permit GCC185 to contribute to stacking and lateral interactions of Golgi cisternae as well as help it function as a vesicle tether...
  25. pmc Multiple routes of protein transport from endosomes to the trans Golgi network
    Suzanne R Pfeffer
    Department of Biochemistry, 279 Campus Drive B400, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    FEBS Lett 583:3811-6. 2009
    ..The identification of distinct molecular requirements for each of these pathways makes it clear that mammalian cells have evolved more complex targeting mechanisms and routes than previously anticipated...
  26. ncbi request reprint Two Rabs for exosome release
    Suzanne R Pfeffer
    Suzanne Pfeffer is at the Department of Biochemistry, Stanford University School of Medicine, 279 Campus Drive B400, Stanford, CA 94305 5307, USA
    Nat Cell Biol 12:3-4. 2010
    ..Rab27A and Rab27B GTPases and two of their cognate effector proteins seem to be needed to drive the physiologically important exosome-release process in certain cell types...
  27. pmc Unconventional secretion by autophagosome exocytosis
    Suzanne R Pfeffer
    Department of Biochemistry, School of Medicine, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 188:451-2. 2010
    ..Medium-chain acyl CoAs are also required and may be important for substrate routing to this pathway...
  28. ncbi request reprint TBC1D20 is a Rab1 GTPase-activating protein that mediates hepatitis C virus replication
    Ella H Sklan
    Department of Medicine, Division of Gastroenterology and Hepatology, Stanford University School of Medicine, Palo Alto, CA 94305 5187, USA
    J Biol Chem 282:36354-61. 2007
    ..These results highlight a novel mechanism by which viruses can hijack host cell machinery and suggest an attractive model whereby the NS5A-TBC1D20 interaction may promote viral membrane-associated RNA replication...
  29. pmc Clues to neuro-degeneration in Niemann-Pick type C disease from global gene expression profiling
    Jonathan V Reddy
    Department of Biochemistry, Stanford University School of Medicine, California, United States of America
    PLoS ONE 1:e19. 2006
    ..The majority of NPC cases are due to mutations in the NPC1 gene. The precise function of this gene is not yet known...
  30. pmc A functional role for the GCC185 golgin in mannose 6-phosphate receptor recycling
    Jonathan V Reddy
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Mol Biol Cell 17:4353-63. 2006
    ..These data assign a specific pathway to an interesting, TGN-localized protein and suggest that GCC185 may participate in the docking of late endosome-derived, Rab9-bearing transport vesicles at the TGN...
  31. pmc Identification of residues in TIP47 essential for Rab9 binding
    John Hanna
    Department of Biochemistry, Stanford University School of Medicine, 279 Campus Drive, Stanford, CA 94305 5307, USA
    Proc Natl Acad Sci U S A 99:7450-4. 2002
    ..These experiments reveal distinct binding domains for the Rab9 GTPase and MPR cytoplasmic domains in the cargo selection protein TIP47...
  32. pmc How the Golgi works: a cisternal progenitor model
    Suzanne R Pfeffer
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Proc Natl Acad Sci U S A 107:19614-8. 2010
    ....
  33. ncbi request reprint Structural clues to Rab GTPase functional diversity
    Suzanne R Pfeffer
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    J Biol Chem 280:15485-8. 2005
    ..This review will highlight what we have learned from crystallographic analysis of this important protein family...
  34. ncbi request reprint Yip3 catalyses the dissociation of endosomal Rab-GDI complexes
    Ulf Sivars
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    Nature 425:856-9. 2003
    ..We propose that the conserved Yip proteins serve as GDI-displacement factors for the targeting of Rab GTPases in eukaryotic cells...
  35. doi request reprint Team effort by TRAPP forces a nucleotide fumble
    Ryan M Nottingham
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Cell 133:1141-3. 2008
    ..Cai et al. (2008) now present structural and biochemical analyses of yeast TRAPPI in a complex with Ypt1p revealing a unique mechanism by which TRAPPI catalyzes guanine nucleotide exchange...
  36. pmc RhoBTB3: a Rho GTPase-family ATPase required for endosome to Golgi transport
    Eric J Espinosa
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Cell 137:938-48. 2009
    ..Because RhoBTB3 interacts with TIP47 on membranes, we propose that it may function to release this cargo selection protein from vesicles to permit their efficient docking and fusion at the Golgi...
  37. ncbi request reprint Self-assembly is important for TIP47 function in mannose 6-phosphate receptor transport
    Paul M Sincock
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Traffic 4:18-25. 2003
    ..However, oligomerization is required for TIP47 stimulation of mannose 6-phosphate receptor transport from endosomes to the trans-Golgi in vivo...
  38. pmc Association of beta-1,3-N-acetylglucosaminyltransferase 1 and beta-1,4-galactosyltransferase 1, trans-Golgi enzymes involved in coupled poly-N-acetyllactosamine synthesis
    Peter L Lee
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Glycobiology 19:655-64. 2009
    ..These results demonstrate that B3GNT1 and B4GALT1 physically associate in vitro and in cultured cells, providing insight into possible mechanisms for regulation of polyLacNAc production...
  39. ncbi request reprint WHAMMing into the Golgi
    Garret L Hayes
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Dev Cell 15:171-2. 2008
    ..WHAMM's membrane localization at the entry face of the Golgi complex is novel for an actin nucleation-promoting factor, and highlights the importance of the cytoskeleton in organizing the secretory pathway...
  40. ncbi request reprint In vitro selection and prediction of TIP47 protein-interaction interfaces
    Alondra Schweizer Burguete
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    Nat Methods 1:55-60. 2004
    ..These methods were validated in comparison with the protein's crystal structure, and provide a powerful means to predict protein-protein interaction interfaces...