Garry P Nolan

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. doi Flow cytometry in the post fluorescence era
    Garry P Nolan
    Stanford University School of Medicine, CA 94305, USA
    Best Pract Res Clin Haematol 24:505-8. 2011
  2. pmc Single-cell mass cytometry adapted to measurements of the cell cycle
    Gregory K Behbehani
    Baxter Laboratory for Stem Cell Biology, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cytometry A 81:552-66. 2012
  3. pmc Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry
    Matthew R Clutter
    Department of Microbiology and Immunology, Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California, USA
    Cytometry A 77:1020-31. 2010
  4. ncbi Characterization of the murine immunological signaling network with phosphospecific flow cytometry
    Peter O Krutzik
    Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, Stanford University, Stanford, CA 94305, USA
    J Immunol 175:2366-73. 2005
  5. ncbi Single cell profiling of potentiated phospho-protein networks in cancer cells
    Jonathan M Irish
    Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, Stanford University, Stanford, CA 94305, USA
    Cell 118:217-28. 2004
  6. pmc Alternate mechanisms of initial pattern recognition drive differential immune responses to related poxviruses
    William E O'Gorman
    Department of Microbiology and Immunology, Baxter Lab in Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell Host Microbe 8:174-85. 2010
  7. pmc Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum
    Sean C Bendall
    Baxter Laboratory in Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA
    Science 332:687-96. 2011
  8. ncbi Kinetics of B cell receptor signaling in human B cell subsets mapped by phosphospecific flow cytometry
    Jonathan M Irish
    Department of Medicine, Oncology Division, Stanford University, Stanford, CA 94305, USA
    J Immunol 177:1581-9. 2006
  9. pmc Differential role of ICAM ligands in determination of human memory T cell differentiation
    Omar D Perez
    The Baxter Laboratory for Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA 94305, USA
    BMC Immunol 8:2. 2007
  10. pmc A platinum-based covalent viability reagent for single-cell mass cytometry
    Harris G Fienberg
    Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California, USA
    Cytometry A 81:467-75. 2012

Collaborators

Detail Information

Publications44

  1. doi Flow cytometry in the post fluorescence era
    Garry P Nolan
    Stanford University School of Medicine, CA 94305, USA
    Best Pract Res Clin Haematol 24:505-8. 2011
    ....
  2. pmc Single-cell mass cytometry adapted to measurements of the cell cycle
    Gregory K Behbehani
    Baxter Laboratory for Stem Cell Biology, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cytometry A 81:552-66. 2012
    ..We applied this to map the cell cycle phases of cells spanning the hematopoietic hierarchy in healthy human bone marrow as a prelude to later studies with cancers and other disorders of this lineage...
  3. pmc Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry
    Matthew R Clutter
    Department of Microbiology and Immunology, Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California, USA
    Cytometry A 77:1020-31. 2010
    ..We anticipate the approach will be broadly applicable to intracellular flow cytometry assays with low signal-to-noise ratios...
  4. ncbi Characterization of the murine immunological signaling network with phosphospecific flow cytometry
    Peter O Krutzik
    Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, Stanford University, Stanford, CA 94305, USA
    J Immunol 175:2366-73. 2005
    ..Thus, simultaneous analysis of the three tiers of the immune system network illustrates the principles by which immune regulation is context dependent and how in vitro culture systems compare with the in vivo environment...
  5. ncbi Single cell profiling of potentiated phospho-protein networks in cancer cells
    Jonathan M Irish
    Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, Stanford University, Stanford, CA 94305, USA
    Cell 118:217-28. 2004
    ..Thus, single cell measurements of phospho-protein responses reveal shifts in signaling potential of a phospho-protein network, allowing for categorizing of cell network phenotypes by multidimensional molecular profiles of signaling...
  6. pmc Alternate mechanisms of initial pattern recognition drive differential immune responses to related poxviruses
    William E O'Gorman
    Department of Microbiology and Immunology, Baxter Lab in Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell Host Microbe 8:174-85. 2010
    ..These data link early immune signaling events to infection outcome and suggest that activation of different pattern-recognition receptors early after infection may be important in determining vaccine efficacy...
  7. pmc Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum
    Sean C Bendall
    Baxter Laboratory in Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA
    Science 332:687-96. 2011
    ..Collectively, such single-cell analyses provide system-wide views of immune signaling in healthy human hematopoiesis, against which drug action and disease can be compared for mechanistic studies and pharmacologic intervention...
  8. ncbi Kinetics of B cell receptor signaling in human B cell subsets mapped by phosphospecific flow cytometry
    Jonathan M Irish
    Department of Medicine, Oncology Division, Stanford University, Stanford, CA 94305, USA
    J Immunol 177:1581-9. 2006
    ..These results provide the first kinetic maps of BCR signaling in primary human B cell subsets and enable new studies of signaling in B cell disorders, such as autoimmunity and cancer...
  9. pmc Differential role of ICAM ligands in determination of human memory T cell differentiation
    Omar D Perez
    The Baxter Laboratory for Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA 94305, USA
    BMC Immunol 8:2. 2007
    ..By applying a multivariate intracellular phospho-proteomic analysis, we demonstrate that LFA-1 differentially activates signaling molecules...
  10. pmc A platinum-based covalent viability reagent for single-cell mass cytometry
    Harris G Fienberg
    Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California, USA
    Cytometry A 81:467-75. 2012
    ..Cisplatin can therefore be used as a viability reagent for a wide range of mass cytometry protocols...
  11. pmc Single-cell phospho-specific flow cytometric analysis demonstrates biochemical and functional heterogeneity in human hematopoietic stem and progenitor compartments
    Kenneth D Gibbs
    Program in Immunology, Stanford University School of Medicine, Stanford, CA, USA Baxter Laboratories for Stem Cell Biology, Stanford University School of Medicine, Stanford, CA, USA
    Blood 117:4226-33. 2011
    ....
  12. ncbi Leukocyte functional antigen 1 lowers T cell activation thresholds and signaling through cytohesin-1 and Jun-activating binding protein 1
    Omar D Perez
    Department of Microbiology and Immunology, and The Baxter Laboratory of Genetic Pharmacology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nat Immunol 4:1083-92. 2003
    ..LFA-1 stimulation lowered the threshold of T cell activation. Thus, LFA-1 signaling contributes to T cell activation and effects T cell differentiation...
  13. pmc The initial phase of an immune response functions to activate regulatory T cells
    William E O'Gorman
    Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305, USA
    J Immunol 183:332-9. 2009
    ..These results indicate that one of the earliest events in a T cell response is the activation of endogenous regulatory cells, potentially to prevent autoimmunity...
  14. doi Multiparameter analysis of intracellular phosphoepitopes in immunophenotyped cell populations by flow cytometry
    Omar D Perez
    Stanford University School of Medicine, Stanford, California, USA
    Curr Protoc Cytom . 2005
    ....
  15. ncbi Novel hematopoietic progenitor populations revealed by direct assessment of GATA1 protein expression and cMPL signaling events
    Garrett C Heffner
    Program in Immunology, Ludwig Center at Stanford, Institute of Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA
    Stem Cells 29:1774-82. 2011
    ....
  16. ncbi Coordinate analysis of murine immune cell surface markers and intracellular phosphoproteins by flow cytometry
    Peter O Krutzik
    Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, Stanford University, Stanford, CA 94305, USA
    J Immunol 175:2357-65. 2005
    ....
  17. pmc B-cell signaling networks reveal a negative prognostic human lymphoma cell subset that emerges during tumor progression
    Jonathan M Irish
    Department of Medicine, Oncology Division, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 107:12747-54. 2010
    ..Both the existence of these LNP cells and their aberrant signaling profiles provide targets for new therapies for follicular lymphoma...
  18. pmc Single-cell mass cytometry for analysis of immune system functional states
    Zach B Bjornson
    Stanford University School of Medicine, Department of Microbiology and Immunology, Baxter Laboratory for Stem Cell Biology, 269 Campus Drive, Stanford, CA 94305 5175, USA
    Curr Opin Immunol 25:484-94. 2013
    ..This review will cover the basics of mass cytometry as well as outline assays developed for the platform that enhance the immunologist's analytical arsenal. ..
  19. ncbi From single cells to deep phenotypes in cancer
    Sean C Bendall
    Baxter Laboratory for Stem Cell Biology, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA
    Nat Biotechnol 30:639-47. 2012
    ....
  20. pmc Decoupling of tumor-initiating activity from stable immunophenotype in HoxA9-Meis1-driven AML
    Kenneth D Gibbs
    Baxter Laboratory in Stem Cell Biology, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell Stem Cell 10:210-7. 2012
    ..Moreover, this suggests that in certain malignancies tumor-initiation activity (or "cancer stemness") can represent a cellular state that exists independently of distinct immunophenotypic definition...
  21. pmc Novel mutations in the inhibitory adaptor protein LNK drive JAK-STAT signaling in patients with myeloproliferative neoplasms
    Stephen T Oh
    Division of Hematology, Department of Medicine, Stanford University School of Medicine, Stanford Cancer Center, 875 Blake Wilbur Dr, Stanford, CA 94305 5821, USA
    Blood 116:988-92. 2010
    ..These findings indicate that JAK-STAT activation due to loss of LNK negative feedback regulation is a novel mechanism of MPN pathogenesis...
  22. ncbi LFA-1 signaling through p44/42 is coupled to perforin degranulation in CD56+CD8+ natural killer cells
    Omar D Perez
    Baxter Laboratory for Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA, USA
    Blood 104:1083-93. 2004
    ..These results identify novel, specific functional consequence of LFA-1-mediated cytolytic activity in perforin-containing human NK subsets...
  23. pmc Stage dependent aberrant regulation of cytokine-STAT signaling in murine systemic lupus erythematosus
    Matthew B Hale
    The Baxter Laboratory of Genetic Pharmacology, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA
    PLoS ONE 4:e6756. 2009
    ....
  24. ncbi Phospho-proteomic immune analysis by flow cytometry: from mechanism to translational medicine at the single-cell level
    Omar D Perez
    The Baxter Laboratory for Genetic Pharmacology, Department of Microbiology and Immunology, Stanford University, School of Medicine, Stanford, CA 94305, USA
    Immunol Rev 210:208-28. 2006
    ..Flow cytometry, as a platform that is well situated in both the research and clinical settings, can contribute to drug discovery as well as having utility for both biomarker and patient-stratification...
  25. ncbi The T cell STAT signaling network is reprogrammed within hours of bacteremia via secondary signals
    Andrew N Hotson
    Department of Microbiology and Immunology, The Baxter Laboratory of Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Immunol 182:7558-68. 2009
    ....
  26. pmc Single-cell profiling identifies aberrant STAT5 activation in myeloid malignancies with specific clinical and biologic correlates
    Nikesh Kotecha
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cancer Cell 14:335-43. 2008
    ..This signature was a specific feature involving JAK-STAT signaling, suggesting a critical role of this pathway in the biological mechanism of this disorder and indicating potential targets for future therapies...
  27. pmc Single-cell trajectory detection uncovers progression and regulatory coordination in human B cell development
    Sean C Bendall
    Baxter Laboratory in Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA Department of Pathology, Stanford University, Stanford, CA 94305, USA
    Cell 157:714-25. 2014
    ..This study provides a comprehensive analysis of human B lymphopoiesis, laying a foundation to apply this approach to other tissues and "corrupted" developmental processes including cancer...
  28. ncbi Analysis of protein phosphorylation and cellular signaling events by flow cytometry: techniques and clinical applications
    Peter O Krutzik
    Department of Molecular Pharmacology, School of Medicine, Stanford University, CA 94305, USA
    Clin Immunol 110:206-21. 2004
    ....
  29. pmc Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes
    Evan W Newell
    Department of Microbiology and Immunology, Stanford University, CA 94305, USA
    Immunity 36:142-52. 2012
    ..This large degree of functional diversity even between cells with the same specificity gives CD8(+) T cells a remarkable degree of flexibility in responding to pathogens...
  30. ncbi Genomic and proteomic analysis reveals a threshold level of MYC required for tumor maintenance
    Catherine M Shachaf
    Department of Medicine and Pathology, Division of Medical Oncology, Stanford University School of Medicine, Stanford University, Stanford, California 94305, USA
    Cancer Res 68:5132-42. 2008
    ..Thus, at the MYC threshold, there is a loss of its ability to maintain tumorigenesis, with associated shifts in gene and protein expression that reestablish cell cycle checkpoints, halt protein translation, and promote apoptosis...
  31. ncbi Activation of the PKB/AKT pathway by ICAM-2
    Omar D Perez
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Immunity 16:51-65. 2002
    ..These results attribute a novel signaling function to ICAM-2 that might suggest mechanisms by which ICAM-2 signals intracellular communication at various immunological synapses...
  32. ncbi Treatment of autoimmune disease by adoptive cellular gene therapy
    Ingo H Tarner
    Department of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, California 94305, USA
    Ann N Y Acad Sci 998:512-9. 2003
    ....
  33. ncbi Flow cytometric analysis of kinase signaling cascades
    Omar D Perez
    The Baxter Laboratory for Genetic Pharmacology, Department of Microbiologyand Immunology, Stanford University School of Medicine, Stanford, CA, USA
    Methods Mol Biol 263:67-94. 2004
    ....
  34. ncbi Luminescent imaging of beta-galactosidase activity in living subjects using sequential reporter-enzyme luminescence
    Thomas S Wehrman
    Baxter Laboratory in Genetic Pharmacology, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nat Methods 3:295-301. 2006
    ..Thus, coupling the properties of FLuc to the advantages of beta-gal permits bioluminescent imaging applications that previously were not possible...
  35. ncbi Conditional protein stabilization via the small molecules Shld-1 and rapamycin increases the signal-to-noise ratio with tet-inducible gene expression
    Gal Almogy
    The Department of Microbiology and Immunology, Stanford University School of Medicine, Palo Alto, CA 94305, USA
    Biotechniques 46:44-50. 2009
    ..The results underscore how investigator-defined regulation at multiple levels of protein expression can attain afiner degree of control over the final expression of introduced genes...
  36. pmc A deep profiler's guide to cytometry
    Sean C Bendall
    Baxter Laboratory in Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA
    Trends Immunol 33:323-32. 2012
    ..Immunophenotyping by mass spectrometry provides the ability to measure >36 proteins at a rate of 1000 cells/s. We review these cytometric technologies, capable of high-content, high-throughput single-cell assays...
  37. pmc Electron microscopy localization and characterization of functionalized composite organic-inorganic SERS nanoparticles on leukemia cells
    Ai Leen Koh
    Department of Materials Science and Engineering, Stanford University, Durand Building Room 139, 496 Lomita Mall, Stanford, CA 94305, USA
    Ultramicroscopy 109:111-21. 2008
    ....
  38. pmc T-cell tropism and the role of ORF66 protein in pathogenesis of varicella-zoster virus infection
    Anne Schaap
    Department of Pediatrics, Stanford University School of Medicine, CA 94305 5208, USA
    J Virol 79:12921-33. 2005
    ..These observations suggest that the ORF66 kinase plays a unique role during infection of T cells and supports VZV T-cell tropism by contributing to immune evasion and enhancing survival of infected T cells...
  39. ncbi Phospho-specific flow cytometry: intersection of immunology and biochemistry at the single-cell level
    Matthew B Hale
    Stanford University, Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, 269 Campus Drive, Stanford, CA 94305, USA
    Curr Opin Mol Ther 8:215-24. 2006
    ....
  40. ncbi Transcending the biomarker mindset: deciphering disease mechanisms at the single cell level
    Erika A Danna
    Stanford NHLBI Proteomics Center, Baxter Laboratory of Genetic Pharmacology, Department of Microbiology and Immunology, School of Medicine, Stanford University, 269 Campus Drive, CCSR 3205, Stanford, CA 94305, USA
    Curr Opin Chem Biol 10:20-7. 2006
    ..Phospho-specific flow cytometry provides a method for the analysis of pathological signaling networks, enabling the investigation of disease mechanisms at the single-cell level...
  41. pmc Structural linkage between ligand discrimination and receptor activation by type I interferons
    Christoph Thomas
    Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 146:621-32. 2011
    ....
  42. pmc Learning signaling network structures with sparsely distributed data
    Karen Sachs
    Department of Microbiology and Immunology, Baxter Laboratory in Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA, USA
    J Comput Biol 16:201-12. 2009
    ....
  43. ncbi Global transcriptional response to interferon is a determinant of HCV treatment outcome and is modified by race
    Xiao Song He
    Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA
    Hepatology 44:352-9. 2006
    ....
  44. ncbi Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry
    Omar D Perez
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305 5175, USA
    Nat Biotechnol 20:155-62. 2002
    ....