Garry Nolan

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. ncbi request reprint What's wrong with drug screening today
    Garry P Nolan
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nat Chem Biol 3:187-91. 2007
  2. pmc Differential role of ICAM ligands in determination of human memory T cell differentiation
    Omar D Perez
    The Baxter Laboratory for Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA 94305, USA
    BMC Immunol 8:2. 2007
  3. ncbi request reprint Phospho-proteomic immune analysis by flow cytometry: from mechanism to translational medicine at the single-cell level
    Omar D Perez
    The Baxter Laboratory for Genetic Pharmacology, Department of Microbiology and Immunology, Stanford University, School of Medicine, Stanford, CA 94305, USA
    Immunol Rev 210:208-28. 2006
  4. ncbi request reprint Global transcriptional response to interferon is a determinant of HCV treatment outcome and is modified by race
    Xiao Song He
    Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA
    Hepatology 44:352-9. 2006
  5. pmc T-cell tropism and the role of ORF66 protein in pathogenesis of varicella-zoster virus infection
    Anne Schaap
    Department of Pediatrics, Stanford University School of Medicine, CA 94305 5208, USA
    J Virol 79:12921-33. 2005
  6. pmc Electron microscopy localization and characterization of functionalized composite organic-inorganic SERS nanoparticles on leukemia cells
    Ai Leen Koh
    Department of Materials Science and Engineering, Stanford University, Durand Building Room 139, 496 Lomita Mall, Stanford, CA 94305, USA
    Ultramicroscopy 109:111-21. 2008
  7. ncbi request reprint Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events
    Peter O Krutzik
    Department of Molecular Pharmacology, School of Medicine, Stanford University, Stanford, California 94305, USA
    Cytometry A 55:61-70. 2003
  8. pmc WebFlow: a software package for high-throughput analysis of flow cytometry data
    Mark M Hammer
    Baxter Laboratory in Genetic Pharmacology, Department of Microbiology, Stanford University, Stanford, California 94305, USA
    Assay Drug Dev Technol 7:44-55. 2009
  9. ncbi request reprint Single-cell phospho-protein analysis by flow cytometry
    Kenneth R Schulz
    Stanford University, Stanford, California, USA
    Curr Protoc Immunol . 2007
  10. ncbi request reprint Transcending the biomarker mindset: deciphering disease mechanisms at the single cell level
    Erika A Danna
    Stanford NHLBI Proteomics Center, Baxter Laboratory of Genetic Pharmacology, Department of Microbiology and Immunology, School of Medicine, Stanford University, 269 Campus Drive, CCSR 3205, Stanford, CA 94305, USA
    Curr Opin Chem Biol 10:20-7. 2006

Collaborators

Detail Information

Publications41

  1. ncbi request reprint What's wrong with drug screening today
    Garry P Nolan
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nat Chem Biol 3:187-91. 2007
    ..Drug screening in the immediate term will be best accomplished by early use of primary cells in which the target of the screen is a network of proteins measured in populations of single cells...
  2. pmc Differential role of ICAM ligands in determination of human memory T cell differentiation
    Omar D Perez
    The Baxter Laboratory for Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA 94305, USA
    BMC Immunol 8:2. 2007
    ..By applying a multivariate intracellular phospho-proteomic analysis, we demonstrate that LFA-1 differentially activates signaling molecules...
  3. ncbi request reprint Phospho-proteomic immune analysis by flow cytometry: from mechanism to translational medicine at the single-cell level
    Omar D Perez
    The Baxter Laboratory for Genetic Pharmacology, Department of Microbiology and Immunology, Stanford University, School of Medicine, Stanford, CA 94305, USA
    Immunol Rev 210:208-28. 2006
    ..Flow cytometry, as a platform that is well situated in both the research and clinical settings, can contribute to drug discovery as well as having utility for both biomarker and patient-stratification...
  4. ncbi request reprint Global transcriptional response to interferon is a determinant of HCV treatment outcome and is modified by race
    Xiao Song He
    Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA
    Hepatology 44:352-9. 2006
    ....
  5. pmc T-cell tropism and the role of ORF66 protein in pathogenesis of varicella-zoster virus infection
    Anne Schaap
    Department of Pediatrics, Stanford University School of Medicine, CA 94305 5208, USA
    J Virol 79:12921-33. 2005
    ..These observations suggest that the ORF66 kinase plays a unique role during infection of T cells and supports VZV T-cell tropism by contributing to immune evasion and enhancing survival of infected T cells...
  6. pmc Electron microscopy localization and characterization of functionalized composite organic-inorganic SERS nanoparticles on leukemia cells
    Ai Leen Koh
    Department of Materials Science and Engineering, Stanford University, Durand Building Room 139, 496 Lomita Mall, Stanford, CA 94305, USA
    Ultramicroscopy 109:111-21. 2008
    ....
  7. ncbi request reprint Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events
    Peter O Krutzik
    Department of Molecular Pharmacology, School of Medicine, Stanford University, Stanford, California 94305, USA
    Cytometry A 55:61-70. 2003
    ....
  8. pmc WebFlow: a software package for high-throughput analysis of flow cytometry data
    Mark M Hammer
    Baxter Laboratory in Genetic Pharmacology, Department of Microbiology, Stanford University, Stanford, California 94305, USA
    Assay Drug Dev Technol 7:44-55. 2009
    ..WebFlow provides a novel platform for quantitative analysis of flow cytometric data from high-throughput drug screening or disease profiling experiments...
  9. ncbi request reprint Single-cell phospho-protein analysis by flow cytometry
    Kenneth R Schulz
    Stanford University, Stanford, California, USA
    Curr Protoc Immunol . 2007
    ..This technique has broad applications to take standard biochemical analysis into primary cells to garner valuable information about signaling events in physiologic settings...
  10. ncbi request reprint Transcending the biomarker mindset: deciphering disease mechanisms at the single cell level
    Erika A Danna
    Stanford NHLBI Proteomics Center, Baxter Laboratory of Genetic Pharmacology, Department of Microbiology and Immunology, School of Medicine, Stanford University, 269 Campus Drive, CCSR 3205, Stanford, CA 94305, USA
    Curr Opin Chem Biol 10:20-7. 2006
    ..Phospho-specific flow cytometry provides a method for the analysis of pathological signaling networks, enabling the investigation of disease mechanisms at the single-cell level...
  11. ncbi request reprint Mapping normal and cancer cell signalling networks: towards single-cell proteomics
    Jonathan M Irish
    Department of Medicine, Oncology Division, Stanford University, Stanford, California 94305, USA
    Nat Rev Cancer 6:146-55. 2006
    ..This form of 'single-cell proteomics' can identify pathways that are activated in therapy-resistant cells and can provide biomarkers for cancer diagnosis and for determining patient prognosis...
  12. ncbi request reprint Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling
    Peter O Krutzik
    Department of Microbiology and Immunology, Baxter Laboratory in Genetic Pharmacology, Stanford University, Stanford, California 94305, USA
    Nat Methods 3:361-8. 2006
    ..We also analyzed IFN-gamma signaling in multiple cell types from primary mouse splenocytes, revealing differences in sensitivity and kinetics between B cells, CD4+ and CD4- T cells and CD11b-hi cells...
  13. pmc Inhibition of HMGcoA reductase by atorvastatin prevents and reverses MYC-induced lymphomagenesis
    Catherine M Shachaf
    Division of Medical Oncology, Department of Medicine, Stanford University, Stanford, CA 94305 5151, USA
    Blood 110:2674-84. 2007
    ..Thus, atorvastatin, by inhibiting HMGcoA reductase, induces changes in phosphoprotein signaling that in turn prevent MYC-induced lymphomagenesis...
  14. doi request reprint Multiparameter analysis of intracellular phosphoepitopes in immunophenotyped cell populations by flow cytometry
    Omar D Perez
    Stanford University School of Medicine, Stanford, California, USA
    Curr Protoc Cytom . 2005
    ....
  15. ncbi request reprint High-content single-cell drug screening with phosphospecific flow cytometry
    Peter O Krutzik
    Department of Microbiology and Immunology, Baxter Laboratory in Genetic Pharmacology, Stanford University, 269 Campus Drive, Stanford, California 94305, USA
    Nat Chem Biol 4:132-42. 2008
    ..Phosphoflow therefore provides a unique platform that can be applied throughout the drug discovery process, from early compound screening to in vivo testing and clinical monitoring of drug efficacy...
  16. doi request reprint The T cell STAT signaling network is reprogrammed within hours of bacteremia via secondary signals
    Andrew N Hotson
    Department of Microbiology and Immunology, The Baxter Laboratory of Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Immunol 182:7558-68. 2009
    ....
  17. ncbi request reprint Characterization of the murine immunological signaling network with phosphospecific flow cytometry
    Peter O Krutzik
    Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, Stanford University, Stanford, CA 94305, USA
    J Immunol 175:2366-73. 2005
    ..Thus, simultaneous analysis of the three tiers of the immune system network illustrates the principles by which immune regulation is context dependent and how in vitro culture systems compare with the in vivo environment...
  18. ncbi request reprint A random peptide library fused to CCR5 for selection of mimetopes expressed on the mammalian cell surface via retroviral vectors
    Roland Wolkowicz
    Department of Microbiology and Immunology, Baxter Laboratory in Genetic Pharmacology, School of Medicine, Stanford University, Stanford, California 94305 5175, USA
    J Biol Chem 280:15195-201. 2005
    ..Applications include the creation of decoys for receptors in cell-cell interactions, screening for molecules that drive ligand expression on target cells in two-cell interaction screens, among other utilities...
  19. ncbi request reprint Activation of the PKB/AKT pathway by ICAM-2
    Omar D Perez
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Immunity 16:51-65. 2002
    ..These results attribute a novel signaling function to ICAM-2 that might suggest mechanisms by which ICAM-2 signals intracellular communication at various immunological synapses...
  20. pmc A novel method for detection of phosphorylation in single cells by surface enhanced Raman scattering (SERS) using composite organic-inorganic nanoparticles (COINs)
    Catherine M Shachaf
    Department of Microbiology and Immunology, Stanford University, Stanford, California, United States of America
    PLoS ONE 4:e5206. 2009
    ..Detection of single cell epitopes has been a mainstay of immunophenotyping for over three decades, primarily using fluorescence techniques for quantitation. Fluorescence has broad overlapping spectra, limiting multiplexing abilities...
  21. ncbi request reprint Flow cytometric analysis of kinase signaling cascades
    Omar D Perez
    The Baxter Laboratory for Genetic Pharmacology, Department of Microbiologyand Immunology, Stanford University School of Medicine, Stanford, CA, USA
    Methods Mol Biol 263:67-94. 2004
    ....
  22. ncbi request reprint Single cell profiling of potentiated phospho-protein networks in cancer cells
    Jonathan M Irish
    Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, Stanford University, Stanford, CA 94305, USA
    Cell 118:217-28. 2004
    ..Thus, single cell measurements of phospho-protein responses reveal shifts in signaling potential of a phospho-protein network, allowing for categorizing of cell network phenotypes by multidimensional molecular profiles of signaling...
  23. ncbi request reprint Coordinate analysis of murine immune cell surface markers and intracellular phosphoproteins by flow cytometry
    Peter O Krutzik
    Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, Stanford University, Stanford, CA 94305, USA
    J Immunol 175:2357-65. 2005
    ....
  24. ncbi request reprint Kinetics of B cell receptor signaling in human B cell subsets mapped by phosphospecific flow cytometry
    Jonathan M Irish
    Department of Medicine, Oncology Division, Stanford University, Stanford, CA 94305, USA
    J Immunol 177:1581-9. 2006
    ..These results provide the first kinetic maps of BCR signaling in primary human B cell subsets and enable new studies of signaling in B cell disorders, such as autoimmunity and cancer...
  25. pmc Alternate mechanisms of initial pattern recognition drive differential immune responses to related poxviruses
    William E O'Gorman
    Department of Microbiology and Immunology, Baxter Lab in Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell Host Microbe 8:174-85. 2010
    ..These data link early immune signaling events to infection outcome and suggest that activation of different pattern-recognition receptors early after infection may be important in determining vaccine efficacy...
  26. pmc Characterization of patient specific signaling via augmentation of Bayesian networks with disease and patient state nodes
    Karen Sachs
    Department of Microbiology and Immunology, Baxter Laboratory in Genetic Pharmacology, Stanford School of Medicine, USA
    Conf Proc IEEE Eng Med Biol Soc 2009:6624-7. 2009
    ..We illustrate this approach with a six phospho-protein, 30,000 cell-per-patient dataset characterizing three comparably diagnosed follicular lymphoma, and show that our approach elucidates signaling differences among them...
  27. pmc Stage dependent aberrant regulation of cytokine-STAT signaling in murine systemic lupus erythematosus
    Matthew B Hale
    The Baxter Laboratory of Genetic Pharmacology, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA
    PLoS ONE 4:e6756. 2009
    ....
  28. ncbi request reprint Leukocyte functional antigen 1 lowers T cell activation thresholds and signaling through cytohesin-1 and Jun-activating binding protein 1
    Omar D Perez
    Department of Microbiology and Immunology, and The Baxter Laboratory of Genetic Pharmacology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nat Immunol 4:1083-92. 2003
    ..LFA-1 stimulation lowered the threshold of T cell activation. Thus, LFA-1 signaling contributes to T cell activation and effects T cell differentiation...
  29. pmc Learning signaling network structures with sparsely distributed data
    Karen Sachs
    Department of Microbiology and Immunology, Baxter Laboratory in Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA, USA
    J Comput Biol 16:201-12. 2009
    ....
  30. pmc Motexafin gadolinium (Gd-Tex) selectively induces apoptosis in HIV-1 infected CD4+ T helper cells
    Omar D Perez
    Department of Microbiology and Immunology, The Baxter Laboratories of Genetic Pharmacology, Stanford University, Stanford, CA 94305 5175, USA
    Proc Natl Acad Sci U S A 99:2270-4. 2002
    ..These findings suggest that Gd-Tex may have therapeutic utility as an anti-HIV agent capable of selectively targeting and removing HIV-infected cells in an infected host...
  31. pmc Single-cell profiling identifies aberrant STAT5 activation in myeloid malignancies with specific clinical and biologic correlates
    Nikesh Kotecha
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cancer Cell 14:335-43. 2008
    ..This signature was a specific feature involving JAK-STAT signaling, suggesting a critical role of this pathway in the biological mechanism of this disorder and indicating potential targets for future therapies...
  32. ncbi request reprint Recognizing a something when your library sees it
    Jean Francois Fortin
    Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, Clinical Sciences Research Center, 269 Campus Drive, Stanford School of Medicine, Stanford, CA 94305, USA
    Chem Biol 9:670-2. 2002
    ..The reports discussed here explore two sides of these issues-generating new library formats and identifying the targets of drug ligands...
  33. ncbi request reprint Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry
    Omar D Perez
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305 5175, USA
    Nat Biotechnol 20:155-62. 2002
    ....
  34. ncbi request reprint Phospho-specific flow cytometry: intersection of immunology and biochemistry at the single-cell level
    Matthew B Hale
    Stanford University, Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, 269 Campus Drive, Stanford, CA 94305, USA
    Curr Opin Mol Ther 8:215-24. 2006
    ....
  35. ncbi request reprint Treatment of autoimmune disease by adoptive cellular gene therapy
    Ingo H Tarner
    Department of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, California 94305, USA
    Ann N Y Acad Sci 998:512-9. 2003
    ....
  36. doi request reprint Genomic and proteomic analysis reveals a threshold level of MYC required for tumor maintenance
    Catherine M Shachaf
    Department of Medicine and Pathology, Division of Medical Oncology, Stanford University School of Medicine, Stanford University, Stanford, California 94305, USA
    Cancer Res 68:5132-42. 2008
    ..Thus, at the MYC threshold, there is a loss of its ability to maintain tumorigenesis, with associated shifts in gene and protein expression that reestablish cell cycle checkpoints, halt protein translation, and promote apoptosis...
  37. pmc Mammalian target of rapamycin controls dendritic cell development downstream of Flt3 ligand signaling
    Taheri Sathaliyawala
    Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY 10032, USA
    Immunity 33:597-606. 2010
    ..Thus, PI3K-mTOR signaling downstream of Flt3L controls DC development, and its restriction by Pten ensures optimal DC pool size and subset composition...
  38. ncbi request reprint Causal protein-signaling networks derived from multiparameter single-cell data
    Karen Sachs
    Biological Engineering Division, Massachusetts Institute of Technology MIT, Cambridge, MA 02139, USA
    Science 308:523-9. 2005
    ..Reconstruction of network models from physiologically relevant primary single cells might be applied to understanding native-state tissue signaling biology, complex drug actions, and dysfunctional signaling in diseased cells...
  39. pmc Single-cell, phosphoepitope-specific analysis demonstrates cell type- and pathway-specific dysregulation of Jak/STAT and MAPK signaling associated with in vivo human immunodeficiency virus type 1 infection
    Andrew W Lee
    Division of Experimental Medicine, University of California at San Francisco, 1001 Potrero Ave, Bldg 3, UCSF Box 1234, San Francisco, CA 94143 1234, USA
    J Virol 82:3702-12. 2008
    ..This understanding of altered monocyte signaling responses that contribute to defective antigen presentation during HIV-1 infection could lead to immunotherapeutic approaches that compensate for the deficiency...
  40. ncbi request reprint Y-box-binding protein 1 confers EGF independence to human mammary epithelial cells
    Isabelle M Berquin
    Department of Pathology, Wake Forest University, Winston Salem, NC 27157, USA
    Oncogene 24:3177-86. 2005
    ..This could represent one of the mechanisms by which YB-1 contributes to breast tumor aggressiveness...
  41. ncbi request reprint Analysis of protein phosphorylation and cellular signaling events by flow cytometry: techniques and clinical applications
    Peter O Krutzik
    Department of Molecular Pharmacology, School of Medicine, Stanford University, CA 94305, USA
    Clin Immunol 110:206-21. 2004
    ....

Research Grants14

  1. Software & Hardware Inference Engines for Automated Determination of Primary Cell
    Garry P Nolan; Fiscal Year: 2010
    ..We will contrast this database with the immune system changes that occur in cancer tumour infiltrating cells using two cancer systems. ..
  2. Software & Hardware Inference Engines for Automated Determination of Primary Cell
    Garry Nolan; Fiscal Year: 2009
    ..We will contrast this database with the immune system changes that occur in cancer tumour infiltrating cells using two cancer systems. ..
  3. Single Cell Phosphoprofiling of Diabetic Immune Cells
    Garry Nolan; Fiscal Year: 2007
    ..This will provide information that could point to new targets for drugs to treat diabetes. ..
  4. INTRACELLULAR T CELL REGULATORS OF HIV-1
    Garry Nolan; Fiscal Year: 2004
    ..The roles of these evolutionarily disparate Rel molecules in T cells will be dissected with regards to their activity upon the HIV-1 enhancer and promoter region. ..