Stefanie Y Nishimura
Affiliation: Stanford University
- Translational diffusion of individual class II MHC membrane proteins in cellsMarija Vrljic
Biophysics Program, Stanford University, Stanford, CA 94305 5080, USA
Biophys J 83:2681-92. 2002..Both analyses show that motion is predominantly Brownian. This study finds no strong evidence for significant confinement of either GPI-linked or native I-E(k) in the plasma membrane of CHO cells...
- Diffusion of lipid-like single-molecule fluorophores in the cell membraneStefanie Y Nishimura
Department of Chemistry and Biophysics Program, Stanford University, Stanford, CA 94305 5080, USA
J Phys Chem B 110:8151-7. 2006....
- Cholesterol depletion induces solid-like regions in the plasma membraneStefanie Y Nishimura
Department of Chemistry, Molecular and Cellular Physiology, and Biophysics Program, Stanford University, Stanford, California 94305 5080, USA
Biophys J 90:927-38. 2006..Cytoskeletal effects appear to be minimal. These results are consistent with a previously described model of solid-like domain formation in the plasma membrane...
- Cholesterol depletion suppresses the translational diffusion of class II major histocompatibility complex proteins in the plasma membraneMarija Vrljic
Biophysics Program, Stanford University, Stanford, California 94305 5080, USA
Biophys J 88:334-47. 2005..That is, cholesterol extraction destroys liquid cholesterol-phospholipid complexes, leaving solid-like high melting phospholipid domains that inhibit the lateral diffusion of membrane components...
- DCDHF fluorophores for single-molecule imaging in cellsSamuel J Lord
Department of Chemistry, Stanford University, Stanford, California 94305, USA
Chemphyschem 10:55-65. 2009..This molecule and its relatives provide a new class of bright photoactivatable small-molecule fluorophores, which are needed for super-resolution imaging schemes that require active control (here turning-on) of single-molecule emission...
- Photophysical properties of acene DCDHF fluorophores: long-wavelength single-molecule emitters designed for cellular imagingSamuel J Lord
Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
J Phys Chem A 111:8934-41. 2007..Finally, we image single copies of the acene DCDHFs diffusing in the plasma membrane of living cells...
- Single-molecule trackingMarija Vrljic
Dept of Chemistry, Stanford University, CA 94305, USA
Methods Mol Biol 398:193-219. 2007....
- Nonlinear optical chromophores as nanoscale emitters for single-molecule spectroscopyKatherine A Willets
Department of Chemistry, Stanford University, Stanford, California 94305, and Department of Chemistry, Kent State University, Kent, Ohio 44242, USA
Acc Chem Res 38:549-56. 2005..Some preliminary applications of the chromophores as single-molecule reporters in cellular and polymer systems are discussed, along with detection of the emitters by two-photon fluorescence...
- Effect of lysozyme adsorption on the interfacial rheology of DPPC and cholesteryl myristate filmsStefanie Y Nishimura
Department of Chemical Engineering, Stanford University, USA
Langmuir 24:11728-33. 2008..Lysozyme adsorption increased the ductility and decreased the isotherm hysteresis for CM/DPPC films...
- Both MHC class II and its GPI-anchored form undergo hop diffusion as observed by single-molecule trackingYasuhiro M Umemura
Membrane Mechanisms Project, International Cooperative Research Project, Japan Science and Technology Agency, Institute for Integrated Cell Material Sciences, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
Biophys J 95:435-50. 2008..These results indicate that the actin-based membrane skeleton influences the diffusion of both transmembrane and GPI-anchored proteins...