Research Topics
Genomes and Genes | WILLIAM J NELSONSummaryAffiliation: Stanford University Country: USA Publications
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Publications
Adaptation of core mechanisms to generate cell polarityW James Nelson
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, California 94305 5435, USA
Nature 422:766-74. 2003....- Convergence of Wnt, beta-catenin, and cadherin pathwaysW James Nelson
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
Science 303:1483-7. 2004..Here, we assemble evidence of possible interrelations between Wnt and other growth factor signaling, beta-catenin functions, and cadherin-mediated adhesion... - Regulation of cell-cell adhesion by the cadherin-catenin complexW James Nelson
Department of Biological Sciences, The James H Clark Center E200 B, 318 Campus Drive, Stanford University, Stanford, CA 94305 5030, USA
Biochem Soc Trans 36:149-55. 2008..In the present article, in vitro protein assembly studies and live-cell studies of de novo cell-cell adhesion are discussed in the context of how the cadherin-catenin complex and the actin cytoskeleton regulate cell-cell adhesion... - Remodeling epithelial cell organization: transitions between front-rear and apical-basal polarityW James Nelson
Department of Biology, Stanford University, Stanford, California 94305, USA
Cold Spring Harb Perspect Biol 1:a000513. 2009..The challenge is to understand how these regulators and effectors are adapted to regulate symmetry breaking processes that generate cell polarities that are specialized for different cellular activities and functions... - Tube morphogenesis: closure, but many openings remainW James Nelson
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305 5435, USA
Trends Cell Biol 13:615-21. 2003..This review provides a summary of these pathways based upon articles published in the Tube Morphogenesis Series in Trends in Cell Biology... - Mum, this bud's for you: where do you want it? Roles for Cdc42 in controlling bud site selection in Saccharomyces cerevisiaeW James Nelson
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305 5435, USA
Bioessays 25:833-6. 2003.... 
Protein trafficking in the exocytic pathway of polarized epithelial cellsW J Nelson
Dept of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305 5435, USA
Trends Cell Biol 11:483-6. 2001..The challenge now is to extend this knowledge to defining molecular mechanisms in detail in vitro and comprehending the development of complex epithelial structures in vivo...- Transcriptional modulation of genes encoding structural characteristics of differentiating enterocytes during development of a polarized epithelium in vitroJennifer M Halbleib
Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA
Mol Biol Cell 18:4261-78. 2007..Coordinated expression of genes encoding components of functional cell structures were often observed indicating temporal control of expression and assembly of multiprotein complexes... - Adenomatous polyposis coli regulates endothelial cell migration independent of roles in beta-catenin signaling and cell-cell adhesionElizabeth S Harris
Department of Biology, The James H Clark Center, Bio X Program, Stanford University, Stanford, CA 94305, USA
Mol Biol Cell 21:2611-23. 2010..These results uncouple different APC functions and show that GSK3 beta/CKI phosphorylation regulates APC clusters and cell migration independently of cell-cell adhesion and beta-catenin transcriptional activity... 
A molecular mechanism directly linking E-cadherin adhesion to initiation of epithelial cell surface polarityLene N Nejsum
Department of Biological Sciences and Department of Molecular and Cellular Physiology, The James H Clark Center, Bio X Program, Stanford University, Stanford, CA 94305, USA
J Cell Biol 178:323-35. 2007....- Fabrication of a dual substrate display to test roles of cell adhesion proteins in vesicle targeting to plasma membrane domainsStephen J Hunt
Department of Biological Sciences, Stanford University, James H Clark Center, 318 Campus Drive, Room E200, Stanford, CA 94305 5430, United States
FEBS Lett 581:4539-43. 2007..These results support the hypothesis that E-cadherin adhesion initiates signaling at the PM resulting in targeted sites for exocytosis... - Deconstructing the cadherin-catenin-actin complexSoichiro Yamada
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
Cell 123:889-901. 2005..These results suggest that the linkage between the cadherin-catenin complex and actin filaments is more dynamic than previously appreciated... - Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell-cell adhesionSoichiro Yamada
Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA
J Cell Biol 178:517-27. 2007..We show that zones of Rac1 and lamellipodia activity and of RhoA and actomyosin contractility are restricted to the periphery of contacting membranes and together drive initiation, expansion, and completion of cell-cell adhesion... - Adenomatous polyposis coli and EB1 localize in close proximity of the mother centriole and EB1 is a functional component of centrosomesRyan K Louie
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
J Cell Sci 117:1117-28. 2004..In summary, our data indicate that APC and EB1 are functional components of mammalian centrosomes and that EB1 is important for anchoring cytoplasmic MT minus ends to the subdistal appendages of the mother centriole... - Immediate-early signaling induced by E-cadherin engagement and adhesionTomas D Perez
Department of Molecular and Cellular Physiology, Stanford University, Stanford, California 94305 5430, USA
J Biol Chem 283:5014-22. 2008.... - AlphaE-catenin regulates actin dynamics independently of cadherin-mediated cell-cell adhesionJacqueline M Benjamin
Cancer Biology Program, Stanford University, Stanford, CA 94305, USA
J Cell Biol 189:339-52. 2010..In contrast, sequestration of cytosolic alphaE-catenin to the plasma membrane reduces membrane dynamics. These results demonstrate that the cytosolic pool of alphaE-catenin regulates actin dynamics independently of cell-cell adhesion... - Alpha-catenin is a molecular switch that binds E-cadherin-beta-catenin and regulates actin-filament assemblyFrauke Drees
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
Cell 123:903-15. 2005..These results indicate a new role for alpha-catenin in local regulation of actin assembly and organization at sites of cadherin-mediated cell-cell adhesion... - Forchlorfenuron alters mammalian septin assembly, organization, and dynamicsQicong Hu
Department of Biology, Stanford University, Stanford, California 94305, USA
J Biol Chem 283:29563-71. 2008..We conclude that FCF is a promising tool to study mammalian septin organization and functions... - A mitotic septin scaffold required for Mammalian chromosome congression and segregationElias T Spiliotis
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305 5435, USA
Science 307:1781-5. 2005..Mammalian septins may thus form a mitotic scaffold for CENP-E and other effectors to coordinate cytokinesis with chromosome congression and segregation... - Spatio-temporal regulation of Rac1 localization and lamellipodia dynamics during epithelial cell-cell adhesionJason S Ehrlich
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, California 94305, USA
Dev Cell 3:259-70. 2002..These results define a role for Rac1 in regulating the rates of initiation and strengthening of cell-cell adhesion... - Parallels between global transcriptional programs of polarizing Caco-2 intestinal epithelial cells in vitro and gene expression programs in normal colon and colon cancerAnnika M Sääf
Department of Biochemistry, Stanford University, Stanford, CA 94305, USA
Mol Biol Cell 18:4245-60. 2007..The full data set is available at http://microarray-pubs.stanford.edu/CACO2... - Two distinct modes of myosin assembly and dynamics during epithelial wound closureMasako Tamada
Department of Biological Sciences, Columbia University, New York, NY 10027, USA
J Cell Biol 176:27-33. 2007..Thus, we suggest that tight junctions serve as attachment points for the actomyosin ring during wound closure and that Rho-kinase is required for localization and activation of the contractile ring... - Adherens and tight junctions: structure, function and connections to the actin cytoskeletonAndrea Hartsock
Departments of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305 5430, USA
Biochim Biophys Acta 1778:660-9. 2008.... - Bench to bedside and back again: molecular mechanisms of alpha-catenin function and roles in tumorigenesisJacqueline M Benjamin
Department of Biological Sciences, Stanford University, 318 Campus Drive, Stanford, CA 94305 5430, USA
Semin Cancer Biol 18:53-64. 2008..This analysis identifies alpha-catenin as a prognostic factor in cancer progression... - Biochemical and structural analysis of alpha-catenin in cell-cell contactsSabine Pokutta
Department of Structural Biology, D100 Fairchild Science Building, Stanford University School of Medicine, Stanford, CA 94305 5126, U S A
Biochem Soc Trans 36:141-7. 2008..Thus alpha-catenin appears to play a central role in cell-cell contact formation... - Regulation of cell motile behavior by crosstalk between cadherin- and integrin-mediated adhesionsNicolas Borghi
Department of Biology, Cancer Biology Program, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 107:13324-9. 2010..These results demonstrate that there is crosstalk between E-cadherin- and integrin-based adhesion complexes and that E-cadherin regulates lamellipodia activity and cell migration directionality, but not cell migration rate... - Fractionation of the epithelial apical junctional complex: reassessment of protein distributions in different substructuresRoger Vogelmann
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305-5435, USA
Mol Biol Cell 16:701-16. 2005.... - APC is a component of an organizing template for cortical microtubule networksAmy Reilein
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine B121, 279 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305 5435, USA
Nat Cell Biol 7:463-73. 2005..Thus, APC is a component of a cortical template that guides microtubule network formation... - Self-organization of an acentrosomal microtubule network at the basal cortex of polarized epithelial cellsAmy Reilein
Department of Biological Sciences, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
J Cell Biol 171:845-55. 2005..These results define minimal parameters for the self-organization of an acentrosomal microtubule network... - Molecular mechanism for orienting membrane and actin dynamics to nascent cell-cell contacts in epithelial cellsMarc D H Hansen
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5426, USA
J Biol Chem 277:45371-6. 2002..This shift may reflect a diffusion trapping mechanism by which these Rac1 complexes are localized to cadherin-mediated cell-cell contacts through an interaction with annexin II... - Cell-adhesion assays: fabrication of an E-cadherin substratum and isolation of lateral and Basal membrane patchesFrauke Drees
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA, USA
Methods Mol Biol 294:303-20. 2005..These membrane patches provide direct access to protein complexes formed on the membrane following cell-cell or cell-ECM adhesion... - Interaction of cadherin with the actin cytoskeletonW James Nelson
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, CA 94305-5435, USA
Novartis Found Symp 269:159-68; discussion 168-77, 223-30. 2005..Here, we describe these protein interactions, and examine critically the evidence that they link cadherins to the actin cytoskeleton... - Role of adenomatous polyposis coli (APC) and microtubules in directional cell migration and neuronal polarizationAngela I M Barth
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
Semin Cell Dev Biol 19:245-51. 2008..This review will summarize these results and then focus on the role of microtubule-binding protein adenomatous polyposis coli (APC) in neuronal polarization and directed migration, and on its regulation by GSK3beta... - Epithelial cell surface polarity: the early stepsLene N Nejsum
Departments of Biology, and Molecular and Cellular Physiology, The James H Clark Center, Bio X Program, Stanford University, 318 Campus Drive E200, Stanford, CA 94305 5430, USA
Front Biosci 14:1088-98. 2009..Shortly hereafter, components of the lateral targeting patch, the Exocyst and the lateral SNARE complex, co-localize with E-cadherin at the forming contact, where they function in specifying the delivery of basal-lateral... - What can humans learn from flies about adenomatous polyposis coli?Angela I M Barth
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, CA 94305 5435, USA
Bioessays 24:771-4. 2002.... - Cell autonomous defects in cortical development revealed by two-color chimera analysisAdam V Kwiatkowski
Department of Biology, Stanford University, Stanford, CA 94305, USA
Mol Cell Neurosci 41:44-50. 2009..This technique could be applied to determine other cell autonomous functions in different stages of cortical development... - Here come the septins: novel polymers that coordinate intracellular functions and organizationElias T Spiliotis
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
J Cell Sci 119:4-10. 2006..Hence, studies of these molecules might provide new insights not only into how cells coordinate their functions, but also into the pathogenesis of cancer and other diseases in which septins are abnormally expressed... - Role of APC and its binding partners in regulating microtubules in mitosisShirin Bahmanyar
Department of Biological Sciences and Molecular Cellular Physiology, Stanford University, Stanford, California 94305 5430, USA
Adv Exp Med Biol 656:65-74. 2009..APC localizes to three key locations in mitosis: kinetochores, the cortex and centrosomes. Here, we discuss possible mechanisms for APC function at these sites and suggest new pathways by which APC mutations promote tumorigenesis... - Disruption of the epithelial apical-junctional complex by Helicobacter pylori CagAManuel R Amieva
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
Science 300:1430-4. 2003..CagA appears to target H. pylori to host cell intercellular junctions and to disrupt junction-mediated functions... - Catenins: playing both sides of the synapseAdam V Kwiatkowski
Department of Biological Sciences, Stanford University, Stanford, CA 94305, United States
Curr Opin Cell Biol 19:551-6. 2007..Here we review the role of catenins in regulating the development of pre- and postsynaptic compartments and function in synaptic plasticity, with particular focus on their role in regulating the actin cytoskeleton... - In vitro and in vivo reconstitution of the cadherin-catenin-actin complex from Caenorhabditis elegansAdam V Kwiatkowski
Department of Biology, Stanford University, 318 Campus Drive, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 107:14591-6. 2010..Our study defines evolutionarily conserved properties of alpha-catenin and suggests that multiple mechanisms regulate alpha-catenin binding to F-actin... - VE-cadherin: at the front, center, and sides of endothelial cell organization and functionElizabeth S Harris
Department of Biology, The James H Clark Center, Bio X Program, Stanford University, Stanford, CA 94305, USA
Curr Opin Cell Biol 22:651-8. 2010..Here we highlight recent work that has advanced our understanding of multiple regulatory and signaling mechanisms that converge on VE-cadherin and have consequences for endothelial barrier function and angiogenic remodeling... - Epithelial polarity requires septin coupling of vesicle transport to polyglutamylated microtubulesElias T Spiliotis
Department of Biology, Stanford University, Stanford, CA 94305, USA
J Cell Biol 180:295-303. 2008..We suggest that septin coupling of the microtubule cytoskeleton to post-Golgi vesicle transport is required for the morphogenesis of polarized epithelia... - Mechanism of recruiting Sec6/8 (exocyst) complex to the apical junctional complex during polarization of epithelial cellsCharles Yeaman
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305 5345, USA
J Cell Sci 117:559-70. 2004.... - Characterization of mammalian Par 6 as a dual-location proteinErin G Cline
Department of Biological Sciences, The James A Clark Center, Stanford University, Stanford, CA 94305 5430, USA
Mol Cell Biol 27:4431-43. 2007..Therefore, we hypothesize that Par 6 in the nucleus acts as a scaffolding protein in nuclear speckle complexes, similar to its role in the cytoplasm... - Dissecting interactions between EB1, microtubules and APC in cortical clusters at the plasma membraneAngela I M Barth
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305 5435, USA
J Cell Sci 115:1583-90. 2002..These results show that localization of APC in cortical clusters is different from that of EB1 at MT plus ends and appears to be independent of EB1... - Re-solving the cadherin-catenin-actin conundrumWilliam I Weis
Department of Structural Biology, Stanford University, Stanford, California 94305, USA
J Biol Chem 281:35593-7. 2006 - Wingless signaling modulates cadherin-mediated cell adhesion in Drosophila imaginal disc cellsAndreas Wodarz
Howard Hughes Medical Institute and Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
J Cell Sci 119:2425-34. 2006.... - Separation of cell-cell adhesion complexes by differential centrifugationRoger Vogelmann
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University of Medicine, Stanford, CA, USA
Methods Mol Biol 370:11-22. 2007.... - Resolving cadherin interactions and binding cooperativity at the single-molecule levelYunxiang Zhang
Department of Physics, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 106:109-14. 2009..We could not detect the formation of cadherin cis dimers, but found that increasing the density of cadherin monomers cooperatively increased the probability of trans adhesive binding... - Cadherins in development: cell adhesion, sorting, and tissue morphogenesisJennifer M Halbleib
Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
Genes Dev 20:3199-214. 2006..Here we examine how different members of the cadherin family act in different developmental contexts, and discuss the mechanisms involved... - Biochemical and structural definition of the l-afadin- and actin-binding sites of alpha-cateninSabine Pokutta
Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305, USA
J Biol Chem 277:18868-74. 2002..alpha-Catenin and the actin-binding protein vinculin share sequence and most likely structural similarity within their actin-binding domains. Despite this homology, actin binding requires additional sequences adjacent to this region... - A septin diffusion barrier at the base of the primary cilium maintains ciliary membrane protein distributionQicong Hu
Department of Biology, Stanford University, Stanford, CA 94305, USA
Science 329:436-9. 2010..Thus, SEPT2 is part of a diffusion barrier at the base of the ciliary membrane and is essential for retaining receptor-signaling pathways in the primary cilium... - Formation of extra centrosomal structures is dependent on beta-cateninShirin Bahmanyar
Department of Biology, Stanford University, Stanford, CA 94305, USA
J Cell Sci 123:3125-35. 2010..These results indicate that beta-catenin is required for centrosome amplification, and mutations in beta-catenin might contribute to the formation of abnormal centrosomes observed in cancers... - Epithelial cell polarity from the outside looking inW James Nelson
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, California 94305-5435, USA
News Physiol Sci 18:143-6. 2003.... - Spatial control of exocytosisElias T Spiliotis
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305-5435, USA
Curr Opin Cell Biol 15:430-7. 2003..Furthermore, local activation of signaling pathways reinforces formation of these patches and might effect global repositioning of the secretory pathway toward sites of localized exocytosis... - Breaking into the epithelial apical-junctional complex--news from pathogen hackersRoger Vogelmann
Department of Molecular and Cellular Physiology, Beckman Center B121, Stanford University School of Medicine, 279 Campus Drive, Stanford, CA 94305-5435, USA
Curr Opin Cell Biol 16:86-93. 2004.... - beta-Catenin is a Nek2 substrate involved in centrosome separationShirin Bahmanyar
Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
Genes Dev 22:91-105. 2008..These results identify beta-catenin as a component of the intercentrosomal linker and define a new function for beta-catenin as a key regulator of mitotic centrosome separation... - Urinary excretion of viable podocytes in health and renal diseaseStefanie U Vogelmann
Division of Nephrology, Department of Medicine, Stanford University School of Medicine, CA 94305, USA
Am J Physiol Renal Physiol 285:F40-8. 2003.... - Neurite outgrowth involves adenomatous polyposis coli protein and beta-cateninViolet Votin
Department of Biological Sciences, Stanford University, Beckman Center B121, Stanford, CA 94305, USA
J Cell Sci 118:5699-708. 2005..These results indicate that APC is involved in both early neurite outgrowth and increased growth of the future axon, and that beta-catenin has a structural role in inhibiting APC function in neurite growth...
Research Grants
- TOPOGENESIS OF NA/K-ATPASE IN POLARIZED EPITHELIAL CELLSW Nelson; Fiscal Year: 2002....
- Cytoskeleton Coordination in Neuronal MorphogenesisW Nelson; Fiscal Year: 2005....
- TOPOGENESIS OF NA/K-ATPASE IN POLARIZED EPITHELIAL CELLSWilliam Nelson; Fiscal Year: 2007....
- Regulation of Cell Migration by the APC-Microtubule ComplexW Nelson; Fiscal Year: 2007....
- SYNAPSE STRUCTURE AND DEVELOPMENTW Nelson; Fiscal Year: 2001..This research will aid in the prevention of psychiatric illness and the development of therapies for afflicted individuals. ..
- TOGENESIS OF NA+,K+-ATPASE IN POLARIZED MDCK EPITHELIALW Nelson; Fiscal Year: 1993....
- Regulation of Cell Migration by the APC-Microtubule ComplexWilliam Nelson; Fiscal Year: 2009..abstract_text> ..