Research Topics
| W E MoernerSummaryAffiliation: Stanford University Country: USA Publications
Research Grants
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Detail Information
Publications
Microscopy beyond the diffraction limit using actively controlled single moleculesW E Moerner
Department of Chemistry, Stanford University, Stanford, California 94305, USA
J Microsc 246:213-20. 2012..Single-molecule active control microscopy provides a powerful window into information about nanoscale structures that was previously unavailable...
New directions in single-molecule imaging and analysisW E Moerner
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 104:12596-602. 2007....- Three-dimensional superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentusMatthew D Lew
Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 108:E1102-10. 2011.... - Translational diffusion of individual class II MHC membrane proteins in cellsMarija Vrljic
Biophysics Program, Stanford University, Stanford, CA 94305-5080, USA
Biophys J 83:2681-92. 2002..Both analyses show that motion is predominantly Brownian. This study finds no strong evidence for significant confinement of either GPI-linked or native I-E(k) in the plasma membrane of CHO cells... - Molecules and methods for super-resolution imagingMichael A Thompson
Department of Chemistry, Stanford University, Stanford, California, USA
Methods Enzymol 475:27-59. 2010..Finally, a new method is described for obtaining three-dimensional super-resolution information using a double-helix point-spread function... - Diffusion of lipid-like single-molecule fluorophores in the cell membraneStefanie Y Nishimura
Department of Chemistry and Biophysics Program, Stanford University, Stanford, CA 94305-5080, USA
J Phys Chem B 110:8151-7. 2006.... - Quantitative multicolor subdiffraction imaging of bacterial protein ultrastructures in three dimensionsAndreas Gahlmann
Department of Chemistry, Stanford University, Stanford, California 94305, United States
Nano Lett 13:987-93. 2013..Surface-relief dielectric phase masks implement a double-helix response at two wavelengths to distinguish two different fluorescent labels and to quantitatively and precisely localize them relative to each other in 3D... - Super-resolution imaging of the nucleoid-associated protein HU in Caulobacter crescentusSteven F Lee
Department of Chemistry, Stanford University, Stanford, California, USA
Biophys J 100:L31-3. 2011..The methods presented in this letter should be of broad applicability in the future study of prokaryotic NAPs... - Sensing cooperativity in ATP hydrolysis for single multisubunit enzymes in solutionYan Jiang
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 108:16962-7. 2011..While a complete alternative microscopic model cannot be defined at present, the addition of subunit-occupancy-dependent cooperativity in hydrolysis yields distributions consistent with the data... - Redox cycling and kinetic analysis of single molecules of solution-phase nitrite reductaseRandall H Goldsmith
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 108:17269-74. 2011..Changes in parameters as a function of substrate concentration are consistent with a random sequential substrate binding mechanism... - Three-dimensional super-resolution imaging of the midplane protein FtsZ in live Caulobacter crescentus cells using astigmatismJulie S Biteen
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
Chemphyschem 13:1007-12. 2012..crescentus cells at different stages of the cell cycle and find that the FtsZ superstructure is dynamic with the cell cycle, forming an open shape during the stalked stage and a dense focus during the pre-divisional stage... - Extending microscopic resolution with single-molecule imaging and active controlMichael A Thompson
Department of Chemistry, Stanford University, Stanford, California 94305, USA
Annu Rev Biophys 41:321-42. 2012..The statistical, imaging, and active-control strategies for achieving superresolution imaging with single molecules are reviewed... - Analytical tools to distinguish the effects of localization error, confinement, and medium elasticity on the velocity autocorrelation functionStephanie C Weber
Department of Biochemistry, Howard Hughes Medical Institute, Stanford University, Stanford, California, USA
Biophys J 102:2443-50. 2012..Finally, we apply our analysis to experimental data sets of chromosomal loci and RNA-protein particles in Escherichia coli... - Cellular inclusion bodies of mutant huntingtin exon 1 obscure small fibrillar aggregate speciesSteffen J Sahl
Department of Chemistry, Stanford University, Stanford, CA, USA
Sci Rep 2:895. 2012..These short structures bear a striking resemblance to fibers described in vitro. Definition of the diverse Htt structures in cells will provide an avenue to link the impact of therapeutic agents to aggregate populations and morphologies... - Exploring protein superstructures and dynamics in live bacterial cells using single-molecule and superresolution imagingJulie S Biteen
Department of Chemistry, University of Michigan, Ann Arbor, MI, USA
Methods Mol Biol 783:139-58. 2011.... - Superresolution imaging of targeted proteins in fixed and living cells using photoactivatable organic fluorophoresHsiao lu D Lee
Department of Chemistry, Stanford University, Stanford, California 94305, USA
J Am Chem Soc 132:15099-101. 2010.... - A spindle-like apparatus guides bacterial chromosome segregationJerod L Ptacin
Department of Developmental Biology, Stanford University School of Medicine, Beckman Center, Stanford, CA 94305, USA
Nat Cell Biol 12:791-8. 2010.... 
Single-molecule trackingMarija Vrljic
Dept of Chemistry, Stanford University, CA 94305, USA
Methods Mol Biol 398:193-219. 2007....- Cholesterol depletion induces solid-like regions in the plasma membraneStefanie Y Nishimura
Department of Chemistry, Molecular and Cellular Physiology, and Biophysics Program, Stanford University, Stanford, California 94305 5080, USA
Biophys J 90:927-38. 2006..Cytoskeletal effects appear to be minimal. These results are consistent with a previously described model of solid-like domain formation in the plasma membrane... - Optical methods for exploring dynamics of single copies of green fluorescent proteinW E Moerner
Department of Chemistry, Stanford University, California 94305 5080, USA
Cytometry 36:232-8. 1999..We compare and contrast the two methods in terms of the ability to follow the complex dynamics of this system... - Visualization of the movement of single histidine kinase molecules in live Caulobacter cellsJ Deich
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 101:15921-6. 2004..We did not detect any directional bias in the motion of the PleC-EYFP molecules, implying that the molecules are not being actively transported... - Suppressing Brownian motion of individual biomolecules in solutionAdam E Cohen
Department of Physics, Stanford University, 381 Via Pueblo Mall, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 103:4362-5. 2006..We also demonstrate trapping and manipulation of single virus particles, lipid vesicles, and fluorescent semiconductor nanocrystals... - Toward nanometer-scale optical photolithography: utilizing the near-field of bowtie optical nanoantennasArvind Sundaramurthy
E L Ginzton Laboratory and Department of Chemistry, Stanford University, California 94305, USA
Nano Lett 6:355-60. 2006.... - Cholesterol depletion suppresses the translational diffusion of class II major histocompatibility complex proteins in the plasma membraneMarija Vrljic
Biophysics Program, Stanford University, Stanford, California 94305 5080, USA
Biophys J 88:334-47. 2005..That is, cholesterol extraction destroys liquid cholesterol-phospholipid complexes, leaving solid-like high melting phospholipid domains that inhibit the lateral diffusion of membrane components... - Probing single biomolecules in solution using the anti-Brownian electrokinetic (ABEL) trapQuan Wang
Department of Chemistry, Stanford University, Stanford, California, USA
Acc Chem Res 45:1955-64. 2012..We determined the rate constants of a model of the underlying kinetics through an analysis of the dwell times of the high/low intensity levels of the fluorophore versus nitrite concentration... - Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objectsMatthew D Lew
Department of Electrical Engineering, Stanford University, 350 Serra Mall, Stanford, California 94305, USA
Opt Lett 36:202-4. 2011..With 99,000 photons detected, the corkscrew PSF achieves a localization precision of 2.7 nm in x, 2.1 nm in y, and 5.7 nm in z... - Watching conformational- and photo-dynamics of single fluorescent proteins in solutionRandall H Goldsmith
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
Nat Chem 2:179-86. 2010.... - Azido push-pull fluorogens photoactivate to produce bright fluorescent labelsSamuel J Lord
Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
J Phys Chem B 114:14157-67. 2010..Lastly, we demonstrate that photoactivated push-pull dyes can insert into bonds of nearby biomolecules, simultaneously forming a covalent bond and becoming fluorescent (fluorogenic photoaffinity labeling)... - Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread functionMichael A Thompson
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
Nano Lett 10:211-8. 2010..The DH-PSF, for the first time, is used to track single quantum dots in aqueous solution and a quantum dot-labeled structure inside a living cell in three dimensions... - Nonlinear optical chromophores as nanoscale emitters for single-molecule spectroscopyKatherine A Willets
Department of Chemistry, Stanford University, Stanford, California 94305, and Department of Chemistry, Kent State University, Kent, Ohio 44242, USA
Acc Chem Res 38:549-56. 2005..Some preliminary applications of the chromophores as single-molecule reporters in cellular and polymer systems are discussed, along with detection of the emitters by two-photon fluorescence... - Single-molecule motions of oligoarginine transporter conjugates on the plasma membrane of Chinese hamster ovary cellsH L Lee
Department of Chemistry, Stanford University, Stanford, California 94305, USA
J Am Chem Soc 130:9364-70. 2008.... - Controlling Brownian motion of single protein molecules and single fluorophores in aqueous bufferAdam E Cohen
Dept of Chemistry, Stanford University, Stanford, CA 94305, USA
Opt Express 16:6941-56. 2008..The feedback latency is set by the finite rate of arrival of photons. We demonstrate trapping of individual molecules of the protein GroEL in buffer, and we show confinement of single fluorophores of the dye Cy3 in water... - A selenium analogue of firefly D-luciferin with red-shifted bioluminescence emissionNicholas R Conley
Department of Chemistry, Stanford University, CA 94305 5080, USA
Angew Chem Int Ed Engl 51:3350-3. 2012..It has a red-shifted bioluminescence emission maximum at 600 nm and is suitable for bioluminescence imaging studies in living subjects... - Simultaneous, accurate measurement of the 3D position and orientation of single moleculesMikael P Backlund
Department of Chemistry and Electrical Engineering, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 109:19087-92. 2012..Furthermore, by averaging many estimations of orientation over different depths, we are able to improve from a lateral SD of 116 (∼4× worse than the photon-limited precision; 28 nm) to 34 nm (within 6 nm of the photon limit)... - An Adaptive Anti-Brownian ELectrokinetic trap with real-time information on single-molecule diffusivity and mobilityQuan Wang
Department of Chemistry, Stanford University, Mail Code 5080, Stanford, California 94305 5080, USA
ACS Nano 5:5792-9. 2011..Moreover, we demonstrate real-time measurement of diffusion coefficient and electrokinetic mobility of trapped objects, using adaptive tuning of the Kalman filter parameters... - Fluorescent saxitoxins for live cell imaging of single voltage-gated sodium ion channels beyond the optical diffraction limitAlison E Ondrus
Department of Chemistry, Stanford University, 333 Campus Drive, Stanford, CA 94305 5080, USA
Chem Biol 19:902-12. 2012.... - Action of the chaperonin GroEL/ES on a non-native substrate observed with single-molecule FRETSo Yeon Kim
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
J Mol Biol 401:553-63. 2010.... - Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread functionMichael A Thompson
Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
Proc Natl Acad Sci U S A 107:17864-71. 2010..The quantitative methods detailed in this paper can be broadly applied to the study of mRNA localization and the dynamics of diverse other biomolecules in a wide variety of cell types... - Micrometer-sized DNA-single-fluorophore-DNA supramolecule: synthesis and single-molecule characterizationJungkyu K Lee
Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
Small 5:2418-23. 2009..This type of triblock structure is a step closer to providing a scaffold for single-molecule electronic devices after metallization of the DNAs... - Photophysical properties of acene DCDHF fluorophores: long-wavelength single-molecule emitters designed for cellular imagingSamuel J Lord
Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
J Phys Chem A 111:8934-41. 2007..Finally, we image single copies of the acene DCDHFs diffusing in the plasma membrane of living cells... - Enhancement of the fluorescence of the blue fluorescent proteins by high pressure or low temperatureKoit Mauring
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA
J Phys Chem B 109:12976-81. 2005..The chromophore's hydrogen-bond equilibrium at room temperature is dominated by entropic effects, while below approximately 200 K the balance is enthalpy-driven... - Fluorescence bleaching reveals asymmetric compartment formation prior to cell division in CaulobacterEllen M Judd
Department of Developmental Biology, Stanford University School of Medicine, 300 Beckman Center, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 100:8235-40. 2003..Monitoring of a fluorescent marker for CtrA showed that the differential degradation of CtrA in the nascent stalk cell compartment occurs only after the cytoplasm is compartmentalized... - A photoactivatable push-pull fluorophore for single-molecule imaging in live cellsSamuel J Lord
Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
J Am Chem Soc 130:9204-5. 2008..This proof-of-principle demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single molecule emission... - Lifetime and spectrally resolved characterization of the photodynamics of single fluorophores in solution using the anti-brownian electrokinetic trapQuan Wang
Department of Chemistry and Department of Electrical Engineering, Stanford University, Stanford, California 94305, United States
J Phys Chem B 117:4641-8. 2013..These results represent a significant advance in the ability to identify and characterize different dynamical states of single molecules in aqueous solution with high precision and millisecond time resolution... - STED microscopy with optimized labeling density reveals 9-fold arrangement of a centriole proteinLana Lau
Department of Chemistry, Stanford University, Stanford, California, USA
Biophys J 102:2926-35. 2012.... - Illuminating single molecules in condensed matterW E Moerner
Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
Science 283:1670-6. 1999.... - Conformational dynamics of single G protein-coupled receptors in solutionSamuel Bockenhauer
Department of Chemistry, Stanford University, Stanford, California 94305, United States
J Phys Chem B 115:13328-38. 2011..The intensity autocorrelations of these faster fluctuations are well-described by stretched exponential functions with a stretching exponent β ~ 0.5, suggesting protein dynamics over a range of time scales... - Single-molecule and superresolution imaging in live bacteria cellsJulie S Biteen
Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
Cold Spring Harb Perspect Biol 2:a000448. 2010.... - Single-molecule spectroscopy and imaging of biomolecules in living cellsSamuel J Lord
Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
Anal Chem 82:2192-203. 2010..This Perspective summarizes the field of live-cell imaging of single biomolecules... - Internal mechanical response of a polymer in solutionAdam E Cohen
Department of Physics, Stanford University, Stanford, California 94305, USA
Phys Rev Lett 98:116001. 2007..We find a nonmonotonic radial dependence of the relaxation time. In contrast with earlier measurements on freely diffusing dsDNA, we observe clear signs of internal hydrodynamic interactions... - Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFPJulie S Biteen
Department of Chemistry, Stanford University, 375 North South Mall, Stanford, California 94305, USA
Nat Methods 5:947-9. 2008..These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging... - DCDHF fluorophores for single-molecule imaging in cellsSamuel J Lord
Department of Chemistry, Stanford University, Stanford, California 94305, USA
Chemphyschem 10:55-65. 2009..This molecule and its relatives provide a new class of bright photoactivatable small-molecule fluorophores, which are needed for super-resolution imaging schemes that require active control (here turning-on) of single-molecule emission... - Exploring the chemical enhancement for surface-enhanced Raman scattering with Au bowtie nanoantennasDavid P Fromm
Department of Chemistry, Stanford University, California 94305, USA
J Chem Phys 124:61101. 2006..This chemical sensitivity of SERS has significant implications for ultra-sensitive detection of single molecules... - Novel fluorophores for single-molecule imagingKatherine A Willets
Department of Chemistry, Stanford University, Stanford, California 94305, USA
J Am Chem Soc 125:1174-5. 2003.... - Single molecules of the bacterial actin MreB undergo directed treadmilling motion in Caulobacter crescentusSo Yeon Kim
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 103:10929-34. 2006..Thus, MreB, like actin, exhibits treadmilling behavior in vivo, and the long MreB structures that have been visualized in multiple bacterial species seem to represent bundles of short filaments that lack a uniform global polarity... - Probing the sequence of conformationally induced polarity changes in the molecular chaperonin GroEL with fluorescence spectroscopySo Yeon Kim
Department of Chemistry, Stanford University, Stanford, California 94305, USA
J Phys Chem B 109:24517-25. 2005.... - Long-wavelength analogue of PRODAN: synthesis and properties of Anthradan, a fluorophore with a 2,6-donor-acceptor anthracene structureZhikuan Lu
Department of Chemistry, Kent State University, Kent, Ohio 44242-0001, USA
J Org Chem 71:9651-7. 2006..Single molecules of these anthracene-containing fluorophores have been imaged in polymer hosts as a proof-of-principle... - Principal-components analysis of shape fluctuations of single DNA moleculesAdam E Cohen
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 104:12622-7. 2007..The modes show strong signs of nonlinear hydrodynamics, a feature of the underlying equations of polymer dynamics that has not previously been reported and is neglected in the widely used Rouse and Zimm approximations... - Polarized fluorescence microscopy of individual and many kinesin motors bound to axonemal microtubulesE J Peterman
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA
Biophys J 81:2851-63. 2001..Our results support models of mechanochemistry that require a state in which both motor domains of a kinesin dimer bind simultaneously with similar orientation with respect to the microtubule...
Research Grants
- Development of Single-Biomolecule Optical ImagingWILLIAM MOERNER; Fiscal Year: 2004..abstract_text> ..
- Actively Controlled and Targeted Single-Molecule Probes for Cellular ImagingLUCILLE SHAPIRO; Fiscal Year: 2010....
- Actively Controlled and Targeted Single-Molecule Probes for Cellular ImagingWILLIAM MOERNER; Fiscal Year: 2009....
- Single-Molecule Fluorophores for Cellular Imaging(RMI)WILLIAM MOERNER; Fiscal Year: 2007..abstract_text> ..
- Development of an Electrokinetic Trap for Single Biomolecules in SolutionWILLIAM MOERNER; Fiscal Year: 2007..It is for this reason that this research represents a new and powerful potential advance for biomedical applications. ..
- Single-Molecule Studies of Chaperonin MechanismWILLIAM MOERNER; Fiscal Year: 2006..The exploratory nature of this application arises from the fact that many of the assays proposed have not been previously utilized to explore chaperonin mechanisms. ..
- Three-Dimensional Superresolution Imaging in Living Cells Using Single-Molecule AWILLIAM E MOERNER; Fiscal Year: 2010....