W E Moerner

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. doi request reprint Single-molecule spectroscopy and imaging over the decades
    W E Moerner
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    Faraday Discuss 184:9-36. 2015
  2. pmc Microscopy beyond the diffraction limit using actively controlled single molecules
    W E Moerner
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Microsc 246:213-20. 2012
  3. pmc New directions in single-molecule imaging and analysis
    W E Moerner
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 104:12596-602. 2007
  4. pmc DCDHF fluorophores for single-molecule imaging in cells
    Samuel J Lord
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    Chemphyschem 10:55-65. 2009
  5. pmc Diffusion of lipid-like single-molecule fluorophores in the cell membrane
    Stefanie Y Nishimura
    Department of Chemistry and Biophysics Program, Stanford University, Stanford, CA 94305 5080, USA
    J Phys Chem B 110:8151-7. 2006
  6. pmc Three-dimensional superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus
    Matthew D Lew
    Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 108:E1102-10. 2011
  7. pmc Photophysical properties of acene DCDHF fluorophores: long-wavelength single-molecule emitters designed for cellular imaging
    Samuel J Lord
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    J Phys Chem A 111:8934-41. 2007
  8. pmc Cholesterol depletion induces solid-like regions in the plasma membrane
    Stefanie Y Nishimura
    Department of Chemistry, Molecular and Cellular Physiology, and Biophysics Program, Stanford University, Stanford, California 94305 5080, USA
    Biophys J 90:927-38. 2006
  9. pmc Cholesterol depletion suppresses the translational diffusion of class II major histocompatibility complex proteins in the plasma membrane
    Marija Vrljic
    Biophysics Program, Stanford University, Stanford, California 94305 5080, USA
    Biophys J 88:334-47. 2005
  10. pmc Probing single biomolecules in solution using the anti-Brownian electrokinetic (ABEL) trap
    Quan Wang
    Department of Chemistry, Stanford University, Stanford, California, USA
    Acc Chem Res 45:1955-64. 2012

Research Grants

Collaborators

Detail Information

Publications82

  1. doi request reprint Single-molecule spectroscopy and imaging over the decades
    W E Moerner
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    Faraday Discuss 184:9-36. 2015
    ....
  2. pmc Microscopy beyond the diffraction limit using actively controlled single molecules
    W E Moerner
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Microsc 246:213-20. 2012
    ..Single-molecule active control microscopy provides a powerful window into information about nanoscale structures that was previously unavailable...
  3. pmc New directions in single-molecule imaging and analysis
    W E Moerner
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 104:12596-602. 2007
    ....
  4. pmc DCDHF fluorophores for single-molecule imaging in cells
    Samuel J Lord
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    Chemphyschem 10:55-65. 2009
    ..This molecule and its relatives provide a new class of bright photoactivatable small-molecule fluorophores, which are needed for super-resolution imaging schemes that require active control (here turning-on) of single-molecule emission...
  5. pmc Diffusion of lipid-like single-molecule fluorophores in the cell membrane
    Stefanie Y Nishimura
    Department of Chemistry and Biophysics Program, Stanford University, Stanford, CA 94305 5080, USA
    J Phys Chem B 110:8151-7. 2006
    ....
  6. pmc Three-dimensional superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus
    Matthew D Lew
    Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 108:E1102-10. 2011
    ....
  7. pmc Photophysical properties of acene DCDHF fluorophores: long-wavelength single-molecule emitters designed for cellular imaging
    Samuel J Lord
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    J Phys Chem A 111:8934-41. 2007
    ..Finally, we image single copies of the acene DCDHFs diffusing in the plasma membrane of living cells...
  8. pmc Cholesterol depletion induces solid-like regions in the plasma membrane
    Stefanie Y Nishimura
    Department of Chemistry, Molecular and Cellular Physiology, and Biophysics Program, Stanford University, Stanford, California 94305 5080, USA
    Biophys J 90:927-38. 2006
    ..Cytoskeletal effects appear to be minimal. These results are consistent with a previously described model of solid-like domain formation in the plasma membrane...
  9. pmc Cholesterol depletion suppresses the translational diffusion of class II major histocompatibility complex proteins in the plasma membrane
    Marija Vrljic
    Biophysics Program, Stanford University, Stanford, California 94305 5080, USA
    Biophys J 88:334-47. 2005
    ..That is, cholesterol extraction destroys liquid cholesterol-phospholipid complexes, leaving solid-like high melting phospholipid domains that inhibit the lateral diffusion of membrane components...
  10. pmc Probing single biomolecules in solution using the anti-Brownian electrokinetic (ABEL) trap
    Quan Wang
    Department of Chemistry, Stanford University, Stanford, California, USA
    Acc Chem Res 45:1955-64. 2012
    ..We determined the rate constants of a model of the underlying kinetics through an analysis of the dwell times of the high/low intensity levels of the fluorophore versus nitrite concentration...
  11. pmc A photoactivatable push-pull fluorophore for single-molecule imaging in live cells
    Samuel J Lord
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    J Am Chem Soc 130:9204-5. 2008
    ..This proof-of-principle demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single molecule emission...
  12. pmc Controlling Brownian motion of single protein molecules and single fluorophores in aqueous buffer
    Adam E Cohen
    Dept of Chemistry, Stanford University, Stanford, CA 94305, USA
    Opt Express 16:6941-56. 2008
    ..The feedback latency is set by the finite rate of arrival of photons. We demonstrate trapping of individual molecules of the protein GroEL in buffer, and we show confinement of single fluorophores of the dye Cy3 in water...
  13. ncbi request reprint Single-molecule tracking
    Marija Vrljic
    Dept of Chemistry, Stanford University, CA 94305, USA
    Methods Mol Biol 398:193-219. 2007
    ....
  14. pmc Translational diffusion of individual class II MHC membrane proteins in cells
    Marija Vrljic
    Biophysics Program, Stanford University, Stanford, CA 94305 5080, USA
    Biophys J 83:2681-92. 2002
    ..Both analyses show that motion is predominantly Brownian. This study finds no strong evidence for significant confinement of either GPI-linked or native I-E(k) in the plasma membrane of CHO cells...
  15. pmc Quantitative multicolor subdiffraction imaging of bacterial protein ultrastructures in three dimensions
    Andreas Gahlmann
    Department of Chemistry, Stanford University, Stanford, California 94305, United States
    Nano Lett 13:987-93. 2013
    ..Surface-relief dielectric phase masks implement a double-helix response at two wavelengths to distinguish two different fluorescent labels and to quantitatively and precisely localize them relative to each other in 3D...
  16. doi request reprint Single-molecule motions enable direct visualization of biomolecular interactions in solution
    Quan Wang
    1 Department of Chemistry, Stanford University, Stanford, California, USA 2 Department of Electrical Engineering, Stanford University, Stanford, California, USA
    Nat Methods 11:555-8. 2014
    ..We demonstrate the approach by resolving a monomer-trimer mixture along a protein dissociation pathway and visualizing the binding-unbinding kinetics of a single DNA molecule. ..
  17. pmc Visualization of the movement of single histidine kinase molecules in live Caulobacter cells
    J Deich
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 101:15921-6. 2004
    ..We did not detect any directional bias in the motion of the PleC-EYFP molecules, implying that the molecules are not being actively transported...
  18. pmc Molecules and methods for super-resolution imaging
    Michael A Thompson
    Department of Chemistry, Stanford University, Stanford, California, USA
    Methods Enzymol 475:27-59. 2010
    ..Finally, a new method is described for obtaining three-dimensional super-resolution information using a double-helix point-spread function...
  19. pmc A polymeric protein anchors the chromosomal origin/ParB complex at a bacterial cell pole
    Grant R Bowman
    Department of Developmental Biology, Stanford University School of Medicine, Beckman Center, Stanford, CA 94305, USA
    Cell 134:945-55. 2008
    ....
  20. pmc Single molecules of the bacterial actin MreB undergo directed treadmilling motion in Caulobacter crescentus
    So Yeon Kim
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 103:10929-34. 2006
    ..Thus, MreB, like actin, exhibits treadmilling behavior in vivo, and the long MreB structures that have been visualized in multiple bacterial species seem to represent bundles of short filaments that lack a uniform global polarity...
  21. ncbi request reprint Nonlinear optical chromophores as nanoscale emitters for single-molecule spectroscopy
    Katherine A Willets
    Department of Chemistry, Stanford University, Stanford, California 94305, and Department of Chemistry, Kent State University, Kent, Ohio 44242, USA
    Acc Chem Res 38:549-56. 2005
    ..Some preliminary applications of the chromophores as single-molecule reporters in cellular and polymer systems are discussed, along with detection of the emitters by two-photon fluorescence...
  22. pmc Sensing cooperativity in ATP hydrolysis for single multisubunit enzymes in solution
    Yan Jiang
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 108:16962-7. 2011
    ..While a complete alternative microscopic model cannot be defined at present, the addition of subunit-occupancy-dependent cooperativity in hydrolysis yields distributions consistent with the data...
  23. pmc Super-resolution imaging of the nucleoid-associated protein HU in Caulobacter crescentus
    Steven F Lee
    Department of Chemistry, Stanford University, Stanford, California, USA
    Biophys J 100:L31-3. 2011
    ..The methods presented in this letter should be of broad applicability in the future study of prokaryotic NAPs...
  24. pmc Internal mechanical response of a polymer in solution
    Adam E Cohen
    Department of Physics, Stanford University, Stanford, California 94305, USA
    Phys Rev Lett 98:116001. 2007
    ..We find a nonmonotonic radial dependence of the relaxation time. In contrast with earlier measurements on freely diffusing dsDNA, we observe clear signs of internal hydrodynamic interactions...
  25. pmc Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function
    Michael A Thompson
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Nano Lett 10:211-8. 2010
    ..The DH-PSF, for the first time, is used to track single quantum dots in aqueous solution and a quantum dot-labeled structure inside a living cell in three dimensions...
  26. pmc Distinct constrictive processes, separated in time and space, divide caulobacter inner and outer membranes
    Ellen M Judd
    Department of Applied Physics, Stanford University School of Medicine, 279 Campus Drive, Beckman Center B300, Stanford, CA 94305 5329, USA
    J Bacteriol 187:6874-82. 2005
    ..Here, we also used FLIP experiments to show that both membrane-bound and periplasmic fluorescent proteins diffuse freely through the FtsZ ring during most of the constriction procession...
  27. pmc Toward nanometer-scale optical photolithography: utilizing the near-field of bowtie optical nanoantennas
    Arvind Sundaramurthy
    E L Ginzton Laboratory and Department of Chemistry, Stanford University, California 94305, USA
    Nano Lett 6:355-60. 2006
    ....
  28. pmc Long-wavelength analogue of PRODAN: synthesis and properties of Anthradan, a fluorophore with a 2,6-donor-acceptor anthracene structure
    Zhikuan Lu
    Department of Chemistry, Kent State University, Kent, Ohio 44242 0001, USA
    J Org Chem 71:9651-7. 2006
    ..Single molecules of these anthracene-containing fluorophores have been imaged in polymer hosts as a proof-of-principle...
  29. pmc Three-dimensional super-resolution imaging of the midplane protein FtsZ in live Caulobacter crescentus cells using astigmatism
    Julie S Biteen
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Chemphyschem 13:1007-12. 2012
    ..crescentus cells at different stages of the cell cycle and find that the FtsZ superstructure is dynamic with the cell cycle, forming an open shape during the stalked stage and a dense focus during the pre-divisional stage...
  30. pmc Redox cycling and kinetic analysis of single molecules of solution-phase nitrite reductase
    Randall H Goldsmith
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 108:17269-74. 2011
    ..Changes in parameters as a function of substrate concentration are consistent with a random sequential substrate binding mechanism...
  31. doi request reprint Photo-induced conformational flexibility in single solution-phase peridinin-chlorophyll-proteins
    Samuel D Bockenhauer
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Phys Chem A 117:8399-406. 2013
    ..In contrast to previous work, we show that this quenching cannot result from isolated photobleaching of Chl. These independent mechanisms arise from distinct conformational changes which maintain relatively stable fluorescence emission. ..
  32. pmc Fluorescent saxitoxins for live cell imaging of single voltage-gated sodium ion channels beyond the optical diffraction limit
    Alison E Ondrus
    Department of Chemistry, Stanford University, 333 Campus Drive, Stanford, CA 94305 5080, USA
    Chem Biol 19:902-12. 2012
    ....
  33. pmc Cellular inclusion bodies of mutant huntingtin exon 1 obscure small fibrillar aggregate species
    Steffen J Sahl
    Department of Chemistry, Stanford University, Stanford, CA, USA
    Sci Rep 2:895. 2012
    ..These short structures bear a striking resemblance to fibers described in vitro. Definition of the diverse Htt structures in cells will provide an avenue to link the impact of therapeutic agents to aggregate populations and morphologies...
  34. pmc Exploring bacterial cell biology with single-molecule tracking and super-resolution imaging
    Andreas Gahlmann
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    Nat Rev Microbiol 12:9-22. 2014
    ....
  35. pmc Single-molecule motions of oligoarginine transporter conjugates on the plasma membrane of Chinese hamster ovary cells
    H L Lee
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Am Chem Soc 130:9364-70. 2008
    ....
  36. ncbi request reprint Optical methods for exploring dynamics of single copies of green fluorescent protein
    W E Moerner
    Department of Chemistry, Stanford University, California 94305 5080, USA
    Cytometry 36:232-8. 1999
    ..We compare and contrast the two methods in terms of the ability to follow the complex dynamics of this system...
  37. pmc Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP
    Julie S Biteen
    Department of Chemistry, Stanford University, 375 North South Mall, Stanford, California 94305, USA
    Nat Methods 5:947-9. 2008
    ..These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging...
  38. doi request reprint Extending microscopic resolution with single-molecule imaging and active control
    Michael A Thompson
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    Annu Rev Biophys 41:321-42. 2012
    ..The statistical, imaging, and active-control strategies for achieving superresolution imaging with single molecules are reviewed...
  39. pmc Analytical tools to distinguish the effects of localization error, confinement, and medium elasticity on the velocity autocorrelation function
    Stephanie C Weber
    Department of Biochemistry, Howard Hughes Medical Institute, Stanford University, Stanford, California, USA
    Biophys J 102:2443-50. 2012
    ..Finally, we apply our analysis to experimental data sets of chromosomal loci and RNA-protein particles in Escherichia coli...
  40. doi request reprint Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions
    Yoav Shechtman
    Department of Chemistry, Stanford University, 375 North South Mall, Stanford, California 94305, United States
    Nano Lett 15:4194-9. 2015
    ..To illustrate, we perform flow profiling in a microfluidic channel and show scan-free tracking of single quantum-dot-labeled phospholipid molecules on the surface of living, thick mammalian cells. ..
  41. pmc A spindle-like apparatus guides bacterial chromosome segregation
    Jerod L Ptacin
    Department of Developmental Biology, Stanford University School of Medicine, Beckman Center, Stanford, CA 94305, USA
    Nat Cell Biol 12:791-8. 2010
    ....
  42. pmc Superresolution imaging of targeted proteins in fixed and living cells using photoactivatable organic fluorophores
    Hsiao lu D Lee
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Am Chem Soc 132:15099-101. 2010
    ....
  43. pmc Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy
    Matthew D Lew
    Departments of Chemistry and Electrical Engineering, Stanford University, Stanford, California 94305, United States
    Nano Lett 13:3967-72. 2013
    ..Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions. ..
  44. pmc Probing the sequence of conformationally induced polarity changes in the molecular chaperonin GroEL with fluorescence spectroscopy
    So Yeon Kim
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Phys Chem B 109:24517-25. 2005
    ....
  45. pmc Cy3-Cy5 covalent heterodimers for single-molecule photoswitching
    Nicholas R Conley
    Department of Chemistry, Stanford University, Stanford, California, USA
    J Phys Chem B 112:11878-80. 2008
    ..As proof of principle, these heterodimers have been applied to super-resolution imaging of the tubular stalk structures of live Caulobacter crescentus bacterial cells...
  46. pmc Exploring protein superstructures and dynamics in live bacterial cells using single-molecule and superresolution imaging
    Julie S Biteen
    Department of Chemistry, University of Michigan, Ann Arbor, MI, USA
    Methods Mol Biol 783:139-58. 2011
    ....
  47. doi request reprint An Adaptive Anti-Brownian ELectrokinetic trap with real-time information on single-molecule diffusivity and mobility
    Quan Wang
    Department of Chemistry, Stanford University, Mail Code 5080, Stanford, California 94305 5080, USA
    ACS Nano 5:5792-9. 2011
    ..Moreover, we demonstrate real-time measurement of diffusion coefficient and electrokinetic mobility of trapped objects, using adaptive tuning of the Kalman filter parameters...
  48. pmc Optimal point spread function design for 3D imaging
    Yoav Shechtman
    Department of Chemistry, Stanford University, 375 North South Mall, Stanford, California 94305, USA
    Phys Rev Lett 113:133902. 2014
    ....
  49. pmc Super-resolution fluorescence of huntingtin reveals growth of globular species into short fibers and coexistence of distinct aggregates
    Whitney C Duim
    Department of Chemistry, Department of Applied Physics, and Department of Biology, Stanford University, Stanford, California 94305, United States
    ACS Chem Biol 9:2767-78. 2014
    ....
  50. pmc Watching conformational- and photo-dynamics of single fluorescent proteins in solution
    Randall H Goldsmith
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Nat Chem 2:179-86. 2010
    ....
  51. pmc Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects
    Matthew D Lew
    Department of Electrical Engineering, Stanford University, 350 Serra Mall, Stanford, California 94305, USA
    Opt Lett 36:202-4. 2011
    ..With 99,000 photons detected, the corkscrew PSF achieves a localization precision of 2.7 nm in x, 2.1 nm in y, and 5.7 nm in z...
  52. pmc Azido push-pull fluorogens photoactivate to produce bright fluorescent labels
    Samuel J Lord
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    J Phys Chem B 114:14157-67. 2010
    ..Lastly, we demonstrate that photoactivated push-pull dyes can insert into bonds of nearby biomolecules, simultaneously forming a covalent bond and becoming fluorescent (fluorogenic photoaffinity labeling)...
  53. pmc Suppressing Brownian motion of individual biomolecules in solution
    Adam E Cohen
    Department of Physics, Stanford University, 381 Via Pueblo Mall, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 103:4362-5. 2006
    ..We also demonstrate trapping and manipulation of single virus particles, lipid vesicles, and fluorescent semiconductor nanocrystals...
  54. ncbi request reprint Enhancement of the fluorescence of the blue fluorescent proteins by high pressure or low temperature
    Koit Mauring
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    J Phys Chem B 109:12976-81. 2005
    ..The chromophore's hydrogen-bond equilibrium at room temperature is dominated by entropic effects, while below approximately 200 K the balance is enthalpy-driven...
  55. pmc Single-molecule orientation measurements with a quadrated pupil
    Adam S Backer
    Institute of Computational and Mathematical Engineering, Stanford, California 94305, USA
    Opt Lett 38:1521-3. 2013
    ....
  56. ncbi request reprint Chromosomal locus tracking with proper accounting of static and dynamic errors
    Mikael P Backlund
    Department of Chemistry, Stanford University, 375 North South Mall, Stanford, California 94305, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 91:062716. 2015
    ..We compare these data to analytical forms of the expected values of the MSD and VAC for a general FBM in the presence of these errors. ..
  57. pmc Single-molecule imaging of Hedgehog pathway protein Smoothened in primary cilia reveals binding events regulated by Patched1
    Ljiljana Milenkovic
    Department of Developmental Biology, Genetics and Bioengineering, Stanford University, Stanford, CA 94305
    Proc Natl Acad Sci U S A 112:8320-5. 2015
    ..Our findings quantify activation-dependent changes in Smo dynamics in cilia and highlight a previously unknown step in Hh pathway activation. ..
  58. pmc Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function
    Yin Loon Lee
    Department of Biology, Stanford School of Medicine, Stanford University, Stanford, CA 94305
    Mol Biol Cell 25:2919-33. 2014
    ..This suggests that Cby1 is required for efficient recruitment of Ahi1, providing a possible molecular mechanism for the ciliogenesis defect in Cby1(-/-) cells...
  59. pmc Small-molecule labeling of live cell surfaces for three-dimensional super-resolution microscopy
    Marissa K Lee
    Department of Chemistry, Stanford University, Stanford, California 94305, United States
    J Am Chem Soc 136:14003-6. 2014
    ..Covalent labeling with the optimized rhodamine spirolactam label provides a general strategy to study the surfaces of living cells with high specificity and resolution down to 10-20 nm. ..
  60. doi request reprint Micrometer-sized DNA-single-fluorophore-DNA supramolecule: synthesis and single-molecule characterization
    Jungkyu K Lee
    Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
    Small 5:2418-23. 2009
    ..This type of triblock structure is a step closer to providing a scaffold for single-molecule electronic devices after metallization of the DNAs...
  61. pmc Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function
    Michael A Thompson
    Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
    Proc Natl Acad Sci U S A 107:17864-71. 2010
    ..The quantitative methods detailed in this paper can be broadly applied to the study of mRNA localization and the dynamics of diverse other biomolecules in a wide variety of cell types...
  62. pmc Action of the chaperonin GroEL/ES on a non-native substrate observed with single-molecule FRET
    So Yeon Kim
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    J Mol Biol 401:553-63. 2010
    ....
  63. pmc Exploring the chemical enhancement for surface-enhanced Raman scattering with Au bowtie nanoantennas
    David P Fromm
    Department of Chemistry, Stanford University, California 94305, USA
    J Chem Phys 124:61101. 2006
    ..This chemical sensitivity of SERS has significant implications for ultra-sensitive detection of single molecules...
  64. pmc Principal-components analysis of shape fluctuations of single DNA molecules
    Adam E Cohen
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 104:12622-7. 2007
    ..The modes show strong signs of nonlinear hydrodynamics, a feature of the underlying equations of polymer dynamics that has not previously been reported and is neglected in the widely used Rouse and Zimm approximations...
  65. pmc Single-molecule spectroscopy reveals photosynthetic LH2 complexes switch between emissive states
    Gabriela S Schlau-Cohen
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 110:10899-903. 2013
    ..This is a previously unobserved, reversible quenching pathway, and is one mechanism through which photosynthetic organisms can adapt to changes in light intensities. ..
  66. pmc A selenium analogue of firefly D-luciferin with red-shifted bioluminescence emission
    Nicholas R Conley
    Department of Chemistry, Stanford University, CA 94305 5080, USA
    Angew Chem Int Ed Engl 51:3350-3. 2012
    ..It has a red-shifted bioluminescence emission maximum at 600 nm and is suitable for bioluminescence imaging studies in living subjects...
  67. pmc Simultaneous, accurate measurement of the 3D position and orientation of single molecules
    Mikael P Backlund
    Department of Chemistry and Electrical Engineering, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 109:19087-92. 2012
    ..Furthermore, by averaging many estimations of orientation over different depths, we are able to improve from a lateral SD of 116 (∼4× worse than the photon-limited precision; 28 nm) to 34 nm (within 6 nm of the photon limit)...
  68. pmc Fluorescence bleaching reveals asymmetric compartment formation prior to cell division in Caulobacter
    Ellen M Judd
    Department of Developmental Biology, Stanford University School of Medicine, 300 Beckman Center, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 100:8235-40. 2003
    ..Monitoring of a fluorescent marker for CtrA showed that the differential degradation of CtrA in the nascent stalk cell compartment occurs only after the cytoplasm is compartmentalized...
  69. doi request reprint Dissecting pigment architecture of individual photosynthetic antenna complexes in solution
    Quan Wang
    Department of Chemistry, Stanford University, Stanford, CA 94305
    Proc Natl Acad Sci U S A 112:13880-5. 2015
    ..The method developed here should find immediate application in understanding the emergent properties of other natural and artificial light-harvesting systems. ..
  70. doi request reprint Single-Molecule Identification of Quenched and Unquenched States of LHCII
    Gabriela S Schlau-Cohen
    Department of Chemistry, Stanford University, Stanford, California 94305, United States
    J Phys Chem Lett 6:860-7. 2015
    ..Furthermore, one of the quenched conformations significantly increases in relative population under environmental conditions mimicking high light. ..
  71. pmc Azimuthal polarization filtering for accurate, precise, and robust single-molecule localization microscopy
    Matthew D Lew
    Departments of Chemistry and Electrical Engineering, Stanford University, Stanford, California 94305, United States
    Nano Lett 14:6407-13. 2014
    ..Furthermore, localization accuracy is maintained even in the presence of aberrations resulting from imaging in mismatched media. ..
  72. pmc Extending single-molecule microscopy using optical Fourier processing
    Adam S Backer
    Institute for Computational and Mathematical Engineering and Department of Chemistry, Stanford University, Stanford, California 94305, United States
    J Phys Chem B 118:8313-29. 2014
    ..Finally, we discuss selected recent applications of Fourier processing methods to measure the orientation, depth, and rotational mobility of single fluorescent molecules. ..
  73. pmc Single-molecule spectroscopy and imaging of biomolecules in living cells
    Samuel J Lord
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    Anal Chem 82:2192-203. 2010
    ..This Perspective summarizes the field of live-cell imaging of single biomolecules...
  74. ncbi request reprint Illuminating single molecules in condensed matter
    W E Moerner
    Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
    Science 283:1670-6. 1999
    ....
  75. pmc Single-molecule and superresolution imaging in live bacteria cells
    Julie S Biteen
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    Cold Spring Harb Perspect Biol 2:a000448. 2010
    ....
  76. pmc STED microscopy with optimized labeling density reveals 9-fold arrangement of a centriole protein
    Lana Lau
    Department of Chemistry, Stanford University, Stanford, California, USA
    Biophys J 102:2926-35. 2012
    ....
  77. pmc Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope
    Mikael P Backlund
    Department of Chemistry, Stanford University, Stanford, CA 94305
    Mol Biol Cell 25:3619-29. 2014
    ..1-30 s), the loci moved with significantly higher subdiffusive mean square displacement exponents than previously reported, which has implications for the application of polymer theory to chromatin motion in eukaryotes...
  78. pmc Conformational dynamics of single G protein-coupled receptors in solution
    Samuel Bockenhauer
    Department of Chemistry, Stanford University, Stanford, California 94305, United States
    J Phys Chem B 115:13328-38. 2011
    ..The intensity autocorrelations of these faster fluctuations are well-described by stretched exponential functions with a stretching exponent β ~ 0.5, suggesting protein dynamics over a range of time scales...
  79. pmc Super-resolution fluorescence imaging with single molecules
    Steffen J Sahl
    Department of Chemistry, Stanford University, Stanford, CA, USA
    Curr Opin Struct Biol 23:778-87. 2013
    ....
  80. doi request reprint Lifetime and spectrally resolved characterization of the photodynamics of single fluorophores in solution using the anti-brownian electrokinetic trap
    Quan Wang
    Department of Chemistry and Department of Electrical Engineering, Stanford University, Stanford, California 94305, United States
    J Phys Chem B 117:4641-8. 2013
    ..These results represent a significant advance in the ability to identify and characterize different dynamical states of single molecules in aqueous solution with high precision and millisecond time resolution...
  81. pmc Polarized fluorescence microscopy of individual and many kinesin motors bound to axonemal microtubules
    E J Peterman
    Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA
    Biophys J 81:2851-63. 2001
    ..Our results support models of mechanochemistry that require a state in which both motor domains of a kinesin dimer bind simultaneously with similar orientation with respect to the microtubule...
  82. ncbi request reprint Novel fluorophores for single-molecule imaging
    Katherine A Willets
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Am Chem Soc 125:1174-5. 2003
    ....

Research Grants4

  1. Development of Single-Biomolecule Optical Imaging
    WILLIAM MOERNER; Fiscal Year: 2004
    ..abstract_text> ..
  2. Single-Molecule Studies of Chaperonin Mechanism
    WILLIAM MOERNER; Fiscal Year: 2006
    ..The exploratory nature of this application arises from the fact that many of the assays proposed have not been previously utilized to explore chaperonin mechanisms. [unreadable] [unreadable] [unreadable]..
  3. Development of an Electrokinetic Trap for Single Biomolecules in Solution
    WILLIAM MOERNER; Fiscal Year: 2008
    ..It is for this reason that this research represents a new and powerful potential advance for biomedical applications. [unreadable] [unreadable] [unreadable]..
  4. Single-Molecule Fluorophores for Cellular Imaging
    WILLIAM MOERNER; Fiscal Year: 2008
    ..abstract_text> ..