D Scott Merrell

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. pmc Iron and pH homeostasis intersect at the level of Fur regulation in the gastric pathogen Helicobacter pylori
    Hanan Gancz
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Infect Immun 74:602-14. 2006
  2. pmc Growth phase-dependent response of Helicobacter pylori to iron starvation
    D Scott Merrell
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    Infect Immun 71:6510-25. 2003
  3. pmc Information overload: assigning genetic functionality in the age of genomics and large-scale screening
    D Scott Merrell
    Stanford University School of Medicine, Dept of Microbiology and Immunology, 299 Campus Drive, Fairchild D051, CA 94305, USA
    Trends Microbiol 10:571-4. 2002
  4. pmc pH-regulated gene expression of the gastric pathogen Helicobacter pylori
    D Scott Merrell
    Department of Microbiology and Immunology, Stanford School of Medicine, California 94305, USA
    Infect Immun 71:3529-39. 2003
  5. pmc Helicobacter pylori apo-Fur regulation appears unconserved across species
    Shana Miles
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    J Microbiol 48:378-86. 2010
  6. pmc Random and site-specific mutagenesis of the Helicobacter pylori ferric uptake regulator provides insight into Fur structure-function relationships
    Jeremy J Gilbreath
    Department of Microbiology and Immunology, Uniformed Services University of Health Sciences, Bethesda, MD, USA
    Mol Microbiol 89:304-23. 2013
  7. pmc Fur activates expression of the 2-oxoglutarate oxidoreductase genes (oorDABC) in Helicobacter pylori
    Jeremy J Gilbreath
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    J Bacteriol 194:6490-7. 2012
  8. pmc Mutagenesis of conserved amino acids of Helicobacter pylori fur reveals residues important for function
    Beth M Carpenter
    Department of Microbiology and Immunology, Uniformed Services University of the Heath Sciences, 4301 Jones Bridge Rd, Bethesda, MD 20814, USA
    J Bacteriol 192:5037-52. 2010
  9. pmc A single nucleotide change affects fur-dependent regulation of sodB in H. pylori
    Beth M Carpenter
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
    PLoS ONE 4:e5369. 2009
  10. pmc Identification and characterization of novel Helicobacter pylori apo-fur-regulated target genes
    Beth M Carpenter
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    J Bacteriol 195:5526-39. 2013

Collaborators

Detail Information

Publications40

  1. pmc Iron and pH homeostasis intersect at the level of Fur regulation in the gastric pathogen Helicobacter pylori
    Hanan Gancz
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Infect Immun 74:602-14. 2006
    ..This fact likely explains the requirement for this regulator for growth and colonization in the stomach...
  2. pmc Growth phase-dependent response of Helicobacter pylori to iron starvation
    D Scott Merrell
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    Infect Immun 71:6510-25. 2003
    ..Finally, bioinformatic analysis of the predicted promoter regions of the differentially regulated genes led to identification of several putative Fur boxes, suggesting a direct role for Fur in iron-dependent regulation of these genes...
  3. pmc Information overload: assigning genetic functionality in the age of genomics and large-scale screening
    D Scott Merrell
    Stanford University School of Medicine, Dept of Microbiology and Immunology, 299 Campus Drive, Fairchild D051, CA 94305, USA
    Trends Microbiol 10:571-4. 2002
    ..This screen and potential new applications of the VAP technique are discussed here...
  4. pmc pH-regulated gene expression of the gastric pathogen Helicobacter pylori
    D Scott Merrell
    Department of Microbiology and Immunology, Stanford School of Medicine, California 94305, USA
    Infect Immun 71:3529-39. 2003
    ..Furthermore, this transcriptional repression was reflected at the level of protein accumulation in the H. pylori cell...
  5. pmc Helicobacter pylori apo-Fur regulation appears unconserved across species
    Shana Miles
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    J Microbiol 48:378-86. 2010
    ..These data suggest that despite the conservation among bacterial Fur proteins, H. pylori Fur contains unique structure/function features that make it novel in comparison to Fur from other species...
  6. pmc Random and site-specific mutagenesis of the Helicobacter pylori ferric uptake regulator provides insight into Fur structure-function relationships
    Jeremy J Gilbreath
    Department of Microbiology and Immunology, Uniformed Services University of Health Sciences, Bethesda, MD, USA
    Mol Microbiol 89:304-23. 2013
    ..Overall, these studies provide novel information regarding the role of these residues in Fur function, and provide mechanistic insight into how H. pylori Fur regulates gene expression in both the iron-bound and apo forms of the protein...
  7. pmc Fur activates expression of the 2-oxoglutarate oxidoreductase genes (oorDABC) in Helicobacter pylori
    Jeremy J Gilbreath
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    J Bacteriol 194:6490-7. 2012
    ..These findings provide novel insight into the genetic regulation of a key metabolic enzyme and add to our understanding of the diverse roles Fur plays in gene regulation in H. pylori...
  8. pmc Mutagenesis of conserved amino acids of Helicobacter pylori fur reveals residues important for function
    Beth M Carpenter
    Department of Microbiology and Immunology, Uniformed Services University of the Heath Sciences, 4301 Jones Bridge Rd, Bethesda, MD 20814, USA
    J Bacteriol 192:5037-52. 2010
    ..This study is the first for H. pylori to analyze the roles of specific amino acid residues of Fur in function and continues to highlight the complexity of Fur regulation in this organism...
  9. pmc A single nucleotide change affects fur-dependent regulation of sodB in H. pylori
    Beth M Carpenter
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
    PLoS ONE 4:e5369. 2009
    ..Taken together these data indicate that this nucleotide residue is important for direct apo-Fur binding to the sodB promoter...
  10. pmc Identification and characterization of novel Helicobacter pylori apo-fur-regulated target genes
    Beth M Carpenter
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    J Bacteriol 195:5526-39. 2013
    ..Together these studies expand the known apo-Fur regulon in H. pylori and characterize the first reported apo-Fur box sequence. ..
  11. pmc The ferric uptake regulator of Helicobacter pylori: a critical player in the battle for iron and colonization of the stomach
    Oscar Q Pich
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Future Microbiol 8:725-38. 2013
    ..In this review, we discuss the main roles of Fur in the biology of H. pylori and highlight the importance of this regulatory protein in the infectious process...
  12. pmc The oligo-acyl lysyl antimicrobial peptide C₁₂K-2β₁₂ exhibits a dual mechanism of action and demonstrates strong in vivo efficacy against Helicobacter pylori
    Morris O Makobongo
    Department of Microbiology and Immunology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    Antimicrob Agents Chemother 56:378-90. 2012
    ..pylori when used as monotherapy. Therefore, OAK peptides may be a valuable resource for therapeutic treatment of H. pylori infection...
  13. pmc Polymorphisms in the intermediate region of VacA impact Helicobacter pylori-induced disease development
    Kathleen R Jones
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd, Bethesda, Maryland 20814, USA
    J Clin Microbiol 49:101-10. 2011
    ..Collectively, these data help explain the impact of the VacA intermediate region on disease and lead to the hypothesis that there are allele-driven interactions between VacA and CagA...
  14. pmc Expanding the Helicobacter pylori genetic toolbox: modification of an endogenous plasmid for use as a transcriptional reporter and complementation vector
    Beth M Carpenter
    Department of Microbiology and Immunology, Uniformed Services University of the Heath Sciences, 4301 Jones Bridge Rd, Bethesda, MD 20814, USA
    Appl Environ Microbiol 73:7506-14. 2007
    ..These studies show the potential of pTM117 as a molecular tool for genetic analysis of H. pylori...
  15. pmc Detailed analysis of Helicobacter pylori Fur-regulated promoters reveals a Fur box core sequence and novel Fur-regulated genes
    Oscar Q Pich
    Department of Microbiology and Immunology, Uniformed Services University of the Heath Sciences, Bethesda, MD 20814, USA
    Mol Microbiol 84:921-41. 2012
    ..Future experiments will determine whether loss of Fur regulation of any of these particular genes contributes to the defects in colonization exhibited by the H. pylori fur mutant...
  16. pmc Detailed in vivo analysis of the role of Helicobacter pylori Fur in colonization and disease
    Shana Miles
    Department of Microbiology and Immunology, Uniformed Services University of the Heath Sciences, Bethesda, Maryland 20814, USA
    Infect Immun 78:3073-82. 2010
    ..pylori Fur is most important at early stages of infection and illustrate the importance of the ability of H. pylori to adapt to its constantly fluctuating environment when it is establishing infection, inflammation, and disease...
  17. ncbi request reprint Phosphorylation-independent effects of CagA during interaction between Helicobacter pylori and T84 polarized monolayers
    Sahar H El-Etr
    Department of Microbiology and Immunology, Stanford University Medical School, Stanford, California 94305, USA
    J Infect Dis 190:1516-23. 2004
    ..These data suggest the presence of distinct functional domains within the CagA protein that play essential roles in protein targeting and alteration of host-cell signaling pathways...
  18. pmc The Helicobacter pylori Ferric Uptake Regulator (Fur) is essential for growth under sodium chloride stress
    Hanan Gancz
    Department of Microbiology and Immunology, Uniformed Services University of Health Sciences, 4301 Jones Bridge Rd, Bethesda, MD 20814, USA
    J Microbiol 49:294-8. 2011
    ..Moreover, we demonstrate that the transcriptional response induced by sodium chloride stress exhibits similarities to that seen under iron depletion...
  19. pmc Polymorphism in the CagA EPIYA motif impacts development of gastric cancer
    Kathleen R Jones
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Marylan 20814, USA
    J Clin Microbiol 47:959-68. 2009
    ....
  20. pmc A core microbiome associated with the peritoneal tumors of pseudomyxoma peritonei
    Jeremy J Gilbreath
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Orphanet J Rare Dis 8:105. 2013
    ..These findings highlighted the possible role for bacteria in PMP disease...
  21. ncbi request reprint Successful culture techniques for Helicobacter species: establishing H. pylori cultures from infected rodents
    Jeannette M Whitmire
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
    Methods Mol Biol 921:29-35. 2012
    ..This chapter describes protocols for establishing H. pylori cultures from infected rodents...
  22. pmc Sodium chloride affects Helicobacter pylori growth and gene expression
    Hanan Gancz
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd, Bethesda, MD 20814, USA
    J Bacteriol 190:4100-5. 2008
    ..Here we characterize the response of H. pylori to temporal changes in sodium chloride concentration and show that growth, cell morphology, survival, and virulence factor expression are all altered by increased salt concentration...
  23. pmc Dietary choice affects Shiga toxin-producing Escherichia coli (STEC) O157:H7 colonization and disease
    Steven D Zumbrun
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814 4799, USA
    Proc Natl Acad Sci U S A 110:E2126-33. 2013
    ..coli O157:H7 may depend, at least in part, on individual diet and/or the capacity of the commensal flora to produce butyrate...
  24. pmc Enterobacterial common antigen mutants of Salmonella enterica serovar Typhimurium establish a persistent infection and provide protection against subsequent lethal challenge
    Jeremy J Gilbreath
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    Infect Immun 80:441-50. 2012
    ..Typhimurium. Thus, wecA mutant (ECA-negative) strains of Salmonella may be useful as live attenuated vaccine strains or as vehicles for heterologous antigen expression...
  25. pmc Change is good: variations in common biological mechanisms in the epsilonproteobacterial genera Campylobacter and Helicobacter
    Jeremy J Gilbreath
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    Microbiol Mol Biol Rev 75:84-132. 2011
    ....
  26. ncbi request reprint Balancing the double-edged sword: metal ion homeostasis and the ulcer bug
    Jeannette M Whitmire
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Curr Med Chem 14:469-78. 2007
    ..pylori colonization and disease and the possibility that these metal homeostasis cascade components may serve as good targets for rational drug design to eradicate H. pylori infection...
  27. pmc Gene expression profiling of Helicobacter pylori reveals a growth-phase-dependent switch in virulence gene expression
    Lucinda J Thompson
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia
    Infect Immun 71:2643-55. 2003
    ....
  28. pmc In vitro antibacterial activity of acyl-lysyl oligomers against Helicobacter pylori
    Morris O Makobongo
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd, Bethesda, Maryland 20814, USA
    Antimicrob Agents Chemother 53:4231-9. 2009
    ..Our results show a significant in vitro effect of C12K-2beta12 against H. pylori and suggest that OAKs may be a valuable resource for the treatment of H. pylori infection...
  29. ncbi request reprint Profiling of microdissected gastric epithelial cells reveals a cell type-specific response to Helicobacter pylori infection
    Anne Mueller
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Gastroenterology 127:1446-62. 2004
    ..We have explored the transcriptional response of the epithelium globally by applying a whole-genome approach to a murine model of infection...
  30. ncbi request reprint Nontraditional therapies to treat Helicobacter pylori infection
    Morris O Makobongo
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd, Bethesda, MD, 20814, USA
    J Microbiol 52:259-72. 2014
    ..Herein we review and discuss each of these novel approaches as a means to target this important gastric pathogen. ..
  31. ncbi request reprint Successful culture techniques for Helicobacter species: verification of Helicobacter identity using 16S rRNA gene sequence analysis
    Jeannette M Whitmire
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
    Methods Mol Biol 921:37-40. 2012
    ..This chapter describes protocols for the verification of putative Helicobacter species' identities using 16S rRNA gene sequence analysis...
  32. ncbi request reprint Successful culture techniques for Helicobacter species: general culture techniques for Helicobacter pylori
    Jeannette M Whitmire
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
    Methods Mol Biol 921:17-27. 2012
    ..This chapter describes successful techniques for culturing H. pylori on selective horse blood agar media and in Brucella broth liquid media. Additionally, the specific growth requirements of other Helicobacter species are noted...
  33. pmc A Tale of Two Toxins: Helicobacter Pylori CagA and VacA Modulate Host Pathways that Impact Disease
    Kathleen R Jones
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences Bethesda, MD, USA
    Front Microbiol 1:115. 2010
    ..Herein we highlight the diverse types of cellular pathways and processes targeted by these important toxins...
  34. pmc Microarray analysis of Shigella flexneri-infected epithelial cells identifies host factors important for apoptosis inhibition
    Christina S Faherty
    Department of Microbiology and Immunology, F, Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    BMC Genomics 11:272. 2010
    ..The goal of this research was to identify host factors that contribute to apoptosis inhibition in infected cells...
  35. ncbi request reprint Frontal and stealth attack strategies in microbial pathogenesis
    D Scott Merrell
    Uniformed Services University of the Health Sciences, Department of Microbiology and Immunology, 4301 Jones Bridge Road, Bethesda, Maryland 20814, USA
    Nature 430:250-6. 2004
    ..This highlights the relevance of continuing to probe the mechanisms used by microorganisms to cause disease, and emphasizes the need for new model systems to advance our understanding of host-pathogen interactions...
  36. ncbi request reprint Acid tolerance of gastrointestinal pathogens
    D Scott Merrell
    Stanford University School of Medicine, Department of Microbiology and Immunology, 299 Campus Drive, Stanford, CA 94305, USA
    Curr Opin Microbiol 5:51-5. 2002
    ..Here, we review recent work on four gastrointestinal pathogens, focusing on studies that reveal both acid-inducible resistance systems and inherent (basal state) acid-resistance systems...
  37. pmc Spatiotemporal analysis of acid adaptation-mediated Vibrio cholerae hyperinfectivity
    Michael J Angelichio
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Infect Immun 72:2405-7. 2004
    ..These studies suggest that the increased infectivity of acid-adapted V. cholerae is due to a more rapid onset of multiplication and/or to an increased multiplication rate within the infant mouse intestine...
  38. pmc Host-induced epidemic spread of the cholera bacterium
    D Scott Merrell
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Nature 417:642-5. 2002
    ....
  39. ncbi request reprint ToxR interferes with CRP-dependent transcriptional activation of ompT in Vibrio cholerae
    Caiyi C Li
    Department of Biochemistry, University of Maryland, Baltimore 21201, USA
    Mol Microbiol 43:1577-89. 2002
    ..Mutations in two regions on CRP (AR1 and AR2) that directly contact RNA polymerase (RNAP) abolish activation, suggesting direct interaction of CRP with RNAP from -310 of the ompT promoter via DNA looping...
  40. ncbi request reprint Identification of novel factors involved in colonization and acid tolerance of Vibrio cholerae
    D Scott Merrell
    Tufts University School of Medicine, Department of Molecular Biology and Microbiology, 136 Harrison Avenue, Boston, MA 02111, USA
    Mol Microbiol 43:1471-91. 2002
    ..Competition assays of these nine acid tolerance response (ATR)-defective mutants revealed that mutations in gshB, hepA and recO result in a 1000-fold reduction in colonization...