Sylvie Lalonde

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. pmc A novel analytical method for in vivo phosphate tracking
    Hong Gu
    Carnegie Institution, Department of Plant Biology, 260 Panama Street, Stanford, CA 94305, USA
    FEBS Lett 580:5885-93. 2006
  2. pmc A membrane protein/signaling protein interaction network for Arabidopsis version AMPv2
    Sylvie Lalonde
    Department of Plant Biology, Carnegie Institution for Science Stanford, CA, USA
    Front Physiol 1:24. 2010
  3. doi request reprint Molecular and cellular approaches for the detection of protein-protein interactions: latest techniques and current limitations
    Sylvie Lalonde
    Carnegie Institution, 260 Panama Street, Stanford, CA 94305, USA
    Plant J 53:610-35. 2008
  4. pmc Protonophore- and pH-insensitive glucose and sucrose accumulation detected by FRET nanosensors in Arabidopsis root tips
    Bhavna Chaudhuri
    Carnegie Institution for Science, Department of Plant Biology, 260 Panama Street, Stanford, CA 94305, USA
    Plant J 56:948-62. 2008
  5. pmc Rapid metabolism of glucose detected with FRET glucose nanosensors in epidermal cells and intact roots of Arabidopsis RNA-silencing mutants
    Karen Deuschle
    Department of Plant Biology, Carnegie Institution, Stanford, California 94305, USA
    Plant Cell 18:2314-25. 2006
  6. pmc Feedback inhibition of ammonium uptake by a phospho-dependent allosteric mechanism in Arabidopsis
    Viviane Lanquar
    Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305, USA
    Plant Cell 21:3610-22. 2009
  7. doi request reprint Optical sensors for monitoring dynamic changes of intracellular metabolite levels in mammalian cells
    Bi Huei Hou
    Carnegie Institution for Science, Stanford, California, USA
    Nat Protoc 6:1818-33. 2011
  8. pmc Sugar transporters for intercellular exchange and nutrition of pathogens
    Li Qing Chen
    Department of Plant Biology, Carnegie Institution for Science, 260 Panama St, Stanford, California 94305, USA
    Nature 468:527-32. 2010
  9. pmc Genetically encoded FRET sensors for visualizing metabolites with subcellular resolution in living cells
    Loren L Looger
    Department of Plant Biology, Carnegie Institution, Stanford, California 94305, USA
    Plant Physiol 138:555-7. 2005
  10. ncbi request reprint Conversion of a putative Agrobacterium sugar-binding protein into a FRET sensor with high selectivity for sucrose
    Ida Lager
    Carnegie Institution, Department of Plant Biology, Stanford, California 94305, USA
    J Biol Chem 281:30875-83. 2006

Collaborators

Detail Information

Publications25

  1. pmc A novel analytical method for in vivo phosphate tracking
    Hong Gu
    Carnegie Institution, Department of Plant Biology, 260 Panama Street, Stanford, CA 94305, USA
    FEBS Lett 580:5885-93. 2006
    ..FLIPPi sensors are suitable for real-time monitoring of P(i) metabolism in living cells, providing a new tool for fluxomics, analysis of pathophysiology or changes of P(i) during cell migration...
  2. pmc A membrane protein/signaling protein interaction network for Arabidopsis version AMPv2
    Sylvie Lalonde
    Department of Plant Biology, Carnegie Institution for Science Stanford, CA, USA
    Front Physiol 1:24. 2010
    ..The robotic screening method established here will enable a systematic analysis of membrane protein interactions in fungi, plants and metazoa...
  3. doi request reprint Molecular and cellular approaches for the detection of protein-protein interactions: latest techniques and current limitations
    Sylvie Lalonde
    Carnegie Institution, 260 Panama Street, Stanford, CA 94305, USA
    Plant J 53:610-35. 2008
    ..597-609). In addition, we discuss the use of interaction data to construct interaction networks and as the basis for the exciting possibility of using to predict interaction surfaces...
  4. pmc Protonophore- and pH-insensitive glucose and sucrose accumulation detected by FRET nanosensors in Arabidopsis root tips
    Bhavna Chaudhuri
    Carnegie Institution for Science, Department of Plant Biology, 260 Panama Street, Stanford, CA 94305, USA
    Plant J 56:948-62. 2008
    ..The root expression maps may help to pinpoint candidate genes for uptake and release of carbohydrates from roots...
  5. pmc Rapid metabolism of glucose detected with FRET glucose nanosensors in epidermal cells and intact roots of Arabidopsis RNA-silencing mutants
    Karen Deuschle
    Department of Plant Biology, Carnegie Institution, Stanford, California 94305, USA
    Plant Cell 18:2314-25. 2006
    ....
  6. pmc Feedback inhibition of ammonium uptake by a phospho-dependent allosteric mechanism in Arabidopsis
    Viviane Lanquar
    Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305, USA
    Plant Cell 21:3610-22. 2009
    ..This novel autoregulatory mechanism is capable of tuning uptake capacity over a wide range of supply levels using an extracellular sensory system, potentially mediated by a transceptor (i.e., transporter and receptor)...
  7. doi request reprint Optical sensors for monitoring dynamic changes of intracellular metabolite levels in mammalian cells
    Bi Huei Hou
    Carnegie Institution for Science, Stanford, California, USA
    Nat Protoc 6:1818-33. 2011
    ..The whole procedure typically takes ∼4 d including seeding and transfection of mammalian cells; the FRET-based analysis of transfected cells takes ∼5 h...
  8. pmc Sugar transporters for intercellular exchange and nutrition of pathogens
    Li Qing Chen
    Department of Plant Biology, Carnegie Institution for Science, 260 Panama St, Stanford, California 94305, USA
    Nature 468:527-32. 2010
    ..The metazoan homologues may be involved in sugar efflux from intestinal, liver, epididymis and mammary cells...
  9. pmc Genetically encoded FRET sensors for visualizing metabolites with subcellular resolution in living cells
    Loren L Looger
    Department of Plant Biology, Carnegie Institution, Stanford, California 94305, USA
    Plant Physiol 138:555-7. 2005
  10. ncbi request reprint Conversion of a putative Agrobacterium sugar-binding protein into a FRET sensor with high selectivity for sucrose
    Ida Lager
    Carnegie Institution, Department of Plant Biology, Stanford, California 94305, USA
    J Biol Chem 281:30875-83. 2006
    ..This novel reagent complements FLIPs for glucose, maltose, ribose, glutamate, and phosphate and will be used for analysis of sucrose-derived carbon flux in bacterial, fungal, plant, and animal cells...
  11. ncbi request reprint Live imaging of glucose homeostasis in nuclei of COS-7 cells
    Marcus Fehr
    Carnegie Institution of Washington, Plant Biology, 260 Panama Street, Stanford, California 94305 4101, USA
    J Fluoresc 14:603-9. 2004
    ....
  12. pmc Genetically encoded sensors for metabolites
    Karen Deuschle
    Carnegie Institution of Washington, Plant Biology, Stanford, California 94305 4101, USA
    Cytometry A 64:3-9. 2005
    ..However, one crucial set of data especially relevant in the case of multicellular organisms is lacking: the knowledge of the spatial and temporal profiles of metabolite levels at cellular and subcellular levels...
  13. pmc An essential role for 14-3-3 proteins in brassinosteroid signal transduction in Arabidopsis
    Srinivas S Gampala
    Department of Plant Biology, Carnegie Institution, Stanford, CA 94305, USA
    Dev Cell 13:177-89. 2007
    ..This study demonstrates that multiple mechanisms are required for BR regulation of gene expression and plant growth...
  14. pmc Uncovering Arabidopsis membrane protein interactome enriched in transporters using mating-based split ubiquitin assays and classification models
    Jin Chen
    Department of Plant Biology, Carnegie Institution for Science Stanford, CA, USA
    Front Plant Sci 3:124. 2012
    ..It also extends and fills gaps in currently available biological networks in plants and allows building a number of hypotheses about processes and mechanisms involving signal-transduction and transport systems...
  15. pmc Shining light on signaling and metabolic networks by genetically encoded biosensors
    Sylvie Lalonde
    Carnegie Institution, Department of Plant Biology, 260 Panama Street, Stanford, California 94305, USA
    Curr Opin Plant Biol 8:574-81. 2005
    ..They represent the basis for cell-based high-throughput assays that are necessary for a systems perspective on plant cell function...
  16. pmc Xanthomonas T3S Effector XopN Suppresses PAMP-Triggered Immunity and Interacts with a Tomato Atypical Receptor-Like Kinase and TFT1
    Jung Gun Kim
    Department of Biology, Stanford University, Stanford, California 94305 5020, USA
    Plant Cell 21:1305-23. 2009
    ..These data provide the basis for a model in which XopN binds to TARK1 to interfere with TARK1-dependent signaling events triggered in response to Xcv infection...
  17. ncbi request reprint Minimally invasive dynamic imaging of ions and metabolites in living cells
    Marcus Fehr
    Carnegie Institution of Washington, Plant Biology, 260 Panama Street, Stanford, California 94305, USA
    Curr Opin Plant Biol 7:345-51. 2004
    ..A major challenge for the future is the application of these nanosensors to quantify metabolite levels in plant cells and tissues...
  18. pmc Ammonium and urea transporter inventory of the selaginella and physcomitrella genomes
    Roberto De Michele
    Department of Plant Biology, Carnegie Institution for Science Stanford, CA, USA
    Front Plant Sci 3:62. 2012
    ..In particular Selaginella, similar to rice, favors the AMT2/MEP family of ammonium transporters over the plant-specific AMT1 type. In comparison, Physcomitrella presents several members belonging to both families...
  19. pmc SUT Sucrose and MST Monosaccharide Transporter Inventory of the Selaginella Genome
    Sylvie Lalonde
    Department of Plant Biology, Carnegie Institution for Science Stanford, CA, USA
    Front Plant Sci 3:24. 2012
    ..We review the current knowledge regarding sugar transport in spikemoss and provide phylogenetic analyses of the complement of MST and SUT homologs in Selaginella (and Physcomitrella)...
  20. pmc Interactions between co-expressed Arabidopsis sucrose transporters in the split-ubiquitin system
    Waltraud X Schulze
    Plant Physiology, Zentrum für Molekularbiologie der Pflanzen ZMBP, Universitat Tubingen, Auf der Morgenstelle1, D 72076 Tubingen, Germany
    BMC Biochem 4:3. 2003
    ..The carriers differ in their kinetic properties. Many transport proteins are known to exist as oligomers. The yeast-based split ubiquitin system can be used to analyze the ability of membrane proteins to interact...
  21. ncbi request reprint Development of a fluorescent nanosensor for ribose
    Ida Lager
    ZMBP Tübingen, Plant Physiology, Tubingen, Germany
    FEBS Lett 553:85-9. 2003
    ..Inhibitor studies suggest that uptake is mediated by a monosaccharide transporter of the GLUT family, however, ribose taken up into the cell was not or only slowly released, indicating irreversibility of uptake...
  22. pmc Fusion to GFP blocks intercellular trafficking of the sucrose transporter SUT1 leading to accumulation in companion cells
    Sylvie Lalonde
    ZMBP Tübingen, Plant Physiology, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    BMC Plant Biol 3:8. 2003
    ..To study mechanisms for trafficking of SUT1, GFP-fusions with and without 3'-UTR were expressed in transgenic plants...
  23. doi request reprint Introns control expression of sucrose transporter LeSUT1 in trichomes, companion cells and in guard cells
    Andreas Weise
    Institute of Molecular Medicine and Cell Research IMMZ, Albert Ludwigs University Freiburg, Stefan Meier Str 17, 79104 Freiburg, Germany
    Plant Mol Biol 68:251-62. 2008
    ..The combination of all introns is required for the full expression pattern in phloem, guard cells and trichomes...
  24. ncbi request reprint Split-ubiquitin system for identifying protein-protein interactions in membrane and full-length proteins
    Christopher Grefen
    Universitat Tubingen, Tubingen, Germany
    Curr Protoc Neurosci . 2007
    ..coli. Protocols for small-scale interaction tests, as well as systematic approaches using sorted bait and prey arrays, are described...
  25. ncbi request reprint In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7 cells by fluorescent nanosensors
    Marcus Fehr
    Zentrum für Molekular biologie der Pflanzen Tübingen, Plant Physiology, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    J Biol Chem 278:19127-33. 2003
    ..The glucose nanosensor provides a novel tool with numerous scientific, medical, and environmental applications...