A M Krensky

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. ncbi request reprint Granulysin: a novel host defense molecule
    Alan M Krensky
    Department of Pediatrics, Stanford University, Stanford, CA, USA
    Am J Transplant 5:1789-92. 2005
  2. pmc Mechanisms of disease: regulation of RANTES (CCL5) in renal disease
    Alan M Krensky
    Division of Immunology and Transplantation Biology in the Department of Pediatrics at Stanford University School of Medicine, Stanford, CA, USA
    Nat Clin Pract Nephrol 3:164-70. 2007
  3. ncbi request reprint Society for Pediatric Research Presidential Address 2002: first principles
    Alan M Krensky
    Department of Pediatrics, Stanford University, Stanford, California 94305 5164, USA
    Pediatr Res 53:513-5. 2003
  4. ncbi request reprint Immunologic tolerance
    A M Krensky
    Department of Pediatrics, Stanford University, CA 94305 5164, USA
    Pediatr Nephrol 16:675-9. 2001
  5. ncbi request reprint Granulysin, a new human cytolytic granule-associated protein with possible involvement in cell-mediated cytotoxicity
    S V Pena
    Department of Pediatrics, Stanford University School of Medicine, CA 94305, USA
    Semin Immunol 9:117-25. 1997
  6. ncbi request reprint A distinct pathway of cell-mediated apoptosis initiated by granulysin
    A A Kaspar
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Immunol 167:350-6. 2001
  7. ncbi request reprint RFLAT-1: a new zinc finger transcription factor that activates RANTES gene expression in T lymphocytes
    A Song
    Department of Pediatrics, Stanford University School of Medicine, California 94305 5208, USA
    Immunity 10:93-103. 1999
  8. ncbi request reprint Granulysin: a novel antimicrobial
    J Kumar
    Department of Medicine, Division of Nephrology, Stanford University, Stanford, CA 94305, USA
    Expert Opin Investig Drugs 10:321-9. 2001
  9. ncbi request reprint Biosynthesis of granulysin, a novel cytolytic molecule
    D A Hanson
    Department of Pediatrics, Stanford University School of Medicine, CA 94305 5208, USA
    Mol Immunol 36:413-22. 1999
  10. ncbi request reprint Granulysin: a novel antimicrobial peptide of cytolytic T lymphocytes and natural killer cells
    A M Krensky
    Department of Pediatrics, Stanford University School of Medicine, CA 94305 5208, USA
    Biochem Pharmacol 59:317-20. 2000

Research Grants

  1. Granulysin: A Novel Cytolytic Molecule
    David Lewis; Fiscal Year: 2006

Collaborators

Detail Information

Publications47

  1. ncbi request reprint Granulysin: a novel host defense molecule
    Alan M Krensky
    Department of Pediatrics, Stanford University, Stanford, CA, USA
    Am J Transplant 5:1789-92. 2005
    ..Recent studies implicate granulysin in cell-mediated cytotoxicity, chemoattraction, immune activation and as a potential diagnostic biomarker for transplant rejection...
  2. pmc Mechanisms of disease: regulation of RANTES (CCL5) in renal disease
    Alan M Krensky
    Division of Immunology and Transplantation Biology in the Department of Pediatrics at Stanford University School of Medicine, Stanford, CA, USA
    Nat Clin Pract Nephrol 3:164-70. 2007
    ..Such complex regulation of biological systems has major implications for the rational design of drugs aimed at increasing or decreasing inflammatory responses in patients...
  3. ncbi request reprint Society for Pediatric Research Presidential Address 2002: first principles
    Alan M Krensky
    Department of Pediatrics, Stanford University, Stanford, California 94305 5164, USA
    Pediatr Res 53:513-5. 2003
  4. ncbi request reprint Immunologic tolerance
    A M Krensky
    Department of Pediatrics, Stanford University, CA 94305 5164, USA
    Pediatr Nephrol 16:675-9. 2001
    ..This review highlights historical perspectives and recent progress in induction and maintenance of immunologic tolerance relevant to pediatric nephrology...
  5. ncbi request reprint Granulysin, a new human cytolytic granule-associated protein with possible involvement in cell-mediated cytotoxicity
    S V Pena
    Department of Pediatrics, Stanford University School of Medicine, CA 94305, USA
    Semin Immunol 9:117-25. 1997
    ..The 9kDa form localizes to dense, highly cytolytic granules and contains the SAPLIP homology domain. A recombinant granulysin protein, corresponding to the 9kDa form, is cytolytic against tumor cell targets...
  6. ncbi request reprint A distinct pathway of cell-mediated apoptosis initiated by granulysin
    A A Kaspar
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Immunol 167:350-6. 2001
    ..Nevertheless, activation of caspase 3 is observed in granulysin-treated cells, suggesting that granulysin activates a novel pathway of CTL- and NK cell-mediated death distinct from granzyme- and death receptor-induced apoptosis...
  7. ncbi request reprint RFLAT-1: a new zinc finger transcription factor that activates RANTES gene expression in T lymphocytes
    A Song
    Department of Pediatrics, Stanford University School of Medicine, California 94305 5208, USA
    Immunity 10:93-103. 1999
    ..While Rel proteins play the dominant role in RANTES gene expression in fibroblasts, RFLAT-1 is a strong transactivator for RANTES in T cells...
  8. ncbi request reprint Granulysin: a novel antimicrobial
    J Kumar
    Department of Medicine, Division of Nephrology, Stanford University, Stanford, CA 94305, USA
    Expert Opin Investig Drugs 10:321-9. 2001
    ..Synthetic peptides derived from granulysin have differential activity against eukaryotic cells and bacteria. Selective bactericidal peptides may have therapeutic roles as novel antibiotics...
  9. ncbi request reprint Biosynthesis of granulysin, a novel cytolytic molecule
    D A Hanson
    Department of Pediatrics, Stanford University School of Medicine, CA 94305 5208, USA
    Mol Immunol 36:413-22. 1999
    ....
  10. ncbi request reprint Granulysin: a novel antimicrobial peptide of cytolytic T lymphocytes and natural killer cells
    A M Krensky
    Department of Pediatrics, Stanford University School of Medicine, CA 94305 5208, USA
    Biochem Pharmacol 59:317-20. 2000
    ..The identification of this novel molecule indicates a broader and perhaps more significant role for T lymphocytes in both innate and acquired antimicrobial defenses...
  11. ncbi request reprint The HLA system, antigen processing and presentation
    A M Krensky
    Department of Pediatrics, Stanford University, California, USA
    Kidney Int Suppl 58:S2-7. 1997
    ..This mature complex is transported to the cell surface for presentation. Alloreactivity is the special case of antigen presentation responsible for transplant rejection...
  12. ncbi request reprint Granulysin expression is a marker for acute rejection and steroid resistance in human renal transplantation
    M M Sarwal
    Department of Pediatrics, Stanford University, Stanford, CA 94305, USA
    Hum Immunol 62:21-31. 2001
    ..Memory CTL abound in steroid resistant grafts and may have a markedly different response to CSA immunotherapy, suggesting a possible mechanism for steroid resistance...
  13. ncbi request reprint Molecular biology of transplantation
    A M Krensky
    Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305 5208, USA
    Nephron 86:260-5. 2000
    ..Such information has proven important in understanding the action of immunosuppressive drugs and will prove useful in the design of new immunotherapies...
  14. ncbi request reprint Processing, subcellular localization, and function of 519 (granulysin), a human late T cell activation molecule with homology to small, lytic, granule proteins
    S V Pena
    Department of Pediatrics, Stanford University School of Medicine, CA 94305, USA
    J Immunol 158:2680-8. 1997
    ..Its lytic activity suggests the involvement of 519 in CTL effector function...
  15. ncbi request reprint Granulysin blocks replication of varicella-zoster virus and triggers apoptosis of infected cells
    A Hata
    Department of Pediatrics, Stanford University School of Medicine, California 94305-5208, USA
    Viral Immunol 14:125-33. 2001
    ..Because granulysin is a product of natural killer cells and T lymphocytes, it is possible that its antiviral activity may act as a mediator of innate and adaptive immune mechanisms...
  16. ncbi request reprint Granulysin, a cytolytic molecule, is also a chemoattractant and proinflammatory activator
    Anmei Deng
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Immunol 174:5243-8. 2005
    ....
  17. pmc Dynamic interplay of transcriptional machinery and chromatin regulates "late" expression of the chemokine RANTES in T lymphocytes
    Yong Tae Ahn
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305 5164, USA
    Mol Cell Biol 27:253-66. 2007
    ..These events recruit RNA polymerase II to the RANTES promoter and are responsible for late expression of RANTES in T lymphocytes. Therefore, KLF13 is a key regulator of late RANTES expression in T lymphocytes...
  18. ncbi request reprint Granulysin
    Carol Clayberger
    Department of Pediatrics, CCSR 2105, Stanford University, Stanford, CA 94305 5164, USA
    Curr Opin Immunol 15:560-5. 2003
    ....
  19. pmc Granulysin-mediated tumor rejection in transgenic mice
    Lisa P Huang
    Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Immunol 178:77-84. 2007
    ..These findings demonstrate for the first time an in vivo effect of GNLY and suggest that GNLY may prove a useful therapeutic modality for the treatment of cancer...
  20. pmc Hemolysis of erythrocytes by granulysin-derived peptides but not by granulysin
    Qing Li
    Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine, 300 Pasteur Dr, Stanford, CA 94305, USA
    Antimicrob Agents Chemother 49:388-97. 2005
    ..The finding that some derivatives of granulysin are hemolytic may have important implications for the design of granulysin-based antimicrobial therapeutics...
  21. ncbi request reprint Human NKT cells express granulysin and exhibit antimycobacterial activity
    Jennifer L Gansert
    Division of Hematology and Oncology and Department of Microbiology and Immunology, University of California, Los Angeles, School of Medicine, Los Angeles, CA 90095, USA
    J Immunol 170:3154-61. 2003
    ..These studies provide evidence that alpha Gal Cer-activated CD1d-restricted T cells can participate in human host defense against M. tuberculosis infection...
  22. pmc Kruppel-like transcription factor 13 regulates T lymphocyte survival in vivo
    Meixia Zhou
    Department of Pediatrics, Stanford University, 300 Pasteur Drive, Palo Alto, CA 94305, USA
    J Immunol 178:5496-504. 2007
    ..Furthermore, EMSA and luciferase reporter assays demonstrated that KLF13 binds to multiple sites within the Bcl-X(L) promoter and results in decreased Bcl-X(L) promoter activity, making KLF13 a negative regulator of BCL-X(L)...
  23. ncbi request reprint A T cell-specific enhancer of the human CD40 ligand gene
    Lisa A Schubert
    Department of Immunology, University of Washington School of Medicine, Seattle, WA 98195, USA
    J Biol Chem 277:7386-95. 2002
    ..Together, these results suggest that the CD40L gene 3'-flanking region acts as a T cell-specific classical transcriptional enhancer by a NF-kappaB p50-dependent mechanism...
  24. pmc Interaction of PRP4 with Kruppel-like factor 13 regulates CCL5 transcription
    Boli Huang
    Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Immunol 178:7081-7. 2007
    ..Finally, knock-down of PRP4 by small interfering RNA markedly decreases CCL5 expression in T lymphocytes. Thus, PRP4-mediated phosphorylation of KLF13 plays a role in the regulation of CCL5 expression in T lymphocytes...
  25. pmc A translational rheostat for RFLAT-1 regulates RANTES expression in T lymphocytes
    Tania Nikolcheva
    Department of Pediatrics, Stanford University School of Medicine, 300 Pasteur Drive, CCSR 2105, Stanford, CA 94305 5164, USA
    J Clin Invest 110:119-26. 2002
    ....
  26. ncbi request reprint Functional domains and DNA-binding sequences of RFLAT-1/KLF13, a Krüppel-like transcription factor of activated T lymphocytes
    An Song
    Department of Pediatrics, Stanford University School of Medicine, Stanford, California 94305 5164, USA
    J Biol Chem 277:30055-65. 2002
    ..The intact CTCCC box on the RANTES promoter is necessary for RFLAT-1-mediated RANTES transcription and is also required for the synergy between RFLAT-1 and NF-kappaB proteins...
  27. pmc CD96 is a leukemic stem cell-specific marker in human acute myeloid leukemia
    Naoki Hosen
    Department of Pathology, Institute of Stem Cell Biology and Regenerative Medicine, Stanford Cancer Center, Stanford University School of Medicine, Stanford, CA 94305 5323, USA
    Proc Natl Acad Sci U S A 104:11008-13. 2007
    ..These results demonstrate that CD96 is a cell surface marker present on many AML-LSC and may serve as an LSC-specific therapeutic target...
  28. ncbi request reprint Dimethyl 2,2-dichlorovinyl phosphate (DDVP) markedly decreases the expression of perforin, granzyme A and granulysin in human NK-92CI cell line
    Qing Li
    Department of Hygiene and Public Health, Nippon Medical School, 1 1 5 Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    Toxicology 213:107-16. 2005
    ..We also found that DDVP have a modest, but a significant inhibitory effect on the transcription of mRNA of perforin, granzyme A and granulysin...
  29. ncbi request reprint Cell selectivity correlates with membrane-specific interactions: a case study on the antimicrobial peptide G15 derived from granulysin
    Ayyalusamy Ramamoorthy
    Department of Chemistry and Biophysics Research Division, University of Michigan, 930 N University Avenue, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1758:154-63. 2006
    ..These results explain the susceptibility of E. coli and the resistance of human Jurkat cells to G15, and may have implications in designing membrane-selective therapeutic agents...
  30. ncbi request reprint Healthy lifestyles are associated with higher levels of perforin, granulysin and granzymes A/B-expressing cells in peripheral blood lymphocytes
    Qing Li
    Department of Hygiene and Public Health, Nippon Medical School, 1 1 5 Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    Prev Med 44:117-23. 2007
    ..In order to explore the underlying mechanism, we investigated the effect of lifestyle on intracellular perforin, granulysin, and granzymes A/B in peripheral blood lymphocytes (PBL)...
  31. ncbi request reprint Cytotoxic CD4+ T cells use granulysin to kill Cryptococcus neoformans, and activation of this pathway is defective in HIV patients
    Chun Fu Zheng
    Department of Microbiology and Infectious Disease, University of Calgary, AB, Canada
    Blood 109:2049-57. 2007
    ..These cells use granulysin as the effector molecule, and priming is dysregulated in HIV-infected patients, which results in defective microbicidal activity...
  32. pmc Decreased serum granulysin levels in childhood tuberculosis which reverse after therapy
    Diana Di Liberto
    Dipartimento di Biopatologia e Metodologie Biomediche, Universita di Palermo, Corso Tukory 211, 90134 Palermo, Italy
    Tuberculosis (Edinb) 87:322-8. 2007
    ..We propose that serum granulysin levels may provide a marker of disease activity in childhood TB and might be useful for monitoring improvement after chemotherapy...
  33. ncbi request reprint Phytoncides (wood essential oils) induce human natural killer cell activity
    Qing Li
    Department of Hygiene and Public Health, Nippon Medical School, Tokyo, Japan
    Immunopharmacol Immunotoxicol 28:319-33. 2006
    ..Taken together, these data indicate that phytoncides significantly enhance human NK activity and this effect is at least partially mediated by induction of intracellular perforin, granzyme A, and granulysin...
  34. pmc Messenger RNA expression of IL-8, FOXP3, and IL-12beta differentiates latent tuberculosis infection from disease
    Bo Wu
    Department of Pediatrics, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA
    J Immunol 178:3688-94. 2007
    ..Thus, measurement of Ag-specific expression of these three genes may offer a specific and noninvasive means of differentiating between latent Mtb infection and TB...
  35. ncbi request reprint Transactivation of the CCL5/RANTES gene by Epstein-Barr virus latent membrane protein 1
    Jun Nosuke Uchihara
    Division of Molecular Virology and Oncology, Graduate School of Medicine, University of the Ryukyus, Nishihara, Japan
    Int J Cancer 114:747-55. 2005
    ..These results indicate that the activation of the NF-kappaB pathway by LMP-1 is required for the activation of CCL5 expression...
  36. ncbi request reprint Identification of epitopes of Mycobacterium tuberculosis 16-kDa protein recognized by human leukocyte antigen-A*0201 CD8(+) T lymphocytes
    Nadia Caccamo
    Department of Biopathology, University of Palermo, Palermo, Italy
    J Infect Dis 186:991-8. 2002
    ..Our data suggest that these antigenic peptides can play a critical role in effective immunity against mycobacterial infection and TB...
  37. ncbi request reprint CD8 T cell-mediated killing of Cryptococcus neoformans requires granulysin and is dependent on CD4 T cells and IL-15
    Ling Ling Ma
    Department of Medical Sciences, University of Calgary, Calgary, Alberta, Canada T2N 4N1
    J Immunol 169:5787-95. 2002
    ..neoformans mitogen is presented to CD4 T cells, which in turn activate accessory cells. The resultant IL-15 activates CD8 T cells to express granulysin, which is responsible for antifungal activity...
  38. ncbi request reprint Selective depression of interferon-gamma and granulysin production with increase of proliferative response by Vgamma9/Vdelta2 T cells in children with tuberculosis
    Francesco Dieli
    Dipartimento di Biopatologia, Universita di Palermo, 90134 Palermo, Italy
    J Infect Dis 186:1835-9. 2002
    ..Disease-associated changes in Vgamma9/Vdelta2 T cell effector functions in patients with tuberculosis are consistent with the possibility that these T cells may play a protective role in immune response against M. tuberculosis infection...
  39. ncbi request reprint CD40 ligand trimer enhances the response of CD8+ T cells to Mycobacterium tuberculosis
    Buka Samten
    Department of Microbiology and Immunology and Center for Pulmonary and Infectious Disease Control, University of Texas Health Center, Tyler, TX 75708, USA
    J Immunol 170:3180-6. 2003
    ..We conclude that CD40LT can enhance CD8(+) T cell effector function in response to M. tuberculosis...
  40. pmc Helicobacter pylori induces RANTES through activation of NF-kappa B
    Naoki Mori
    Department of Virology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan
    Infect Immun 71:3748-56. 2003
    ..pylori induced NF-kappa B activation through an intracellular signaling pathway that involved IKK and NIK, leading to RANTES gene transcription. RANTES induction by H. pylori may play an important role in gastric inflammation...
  41. ncbi request reprint Intracellular mediators of granulysin-induced cell death
    Satoshi Okada
    Division of Immunology and Transplantation Biology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA
    J Immunol 171:2556-62. 2003
    ..Finally, an increase in intracellular glutathione protects target cells from granulysin-induced lysis, indicating the importance of the redox state in granulysin-mediated cell death...
  42. ncbi request reprint Elevated expression of CCL5/RANTES in adult T-cell leukemia cells: possible transactivation of the CCL5 gene by human T-cell leukemia virus type I tax
    Naoki Mori
    Division of Molecular Virology and Oncology, Graduate School of Medicine, University of the Ryukyus, Nishihara, Japan
    Int J Cancer 111:548-57. 2004
    ..Aberrant expression of CCL5 by HTLV-I-infected T cells may impact on the pathophysiology of HTLV-I-associated diseases...
  43. ncbi request reprint Gamma delta T cells inhibit in vitro growth of the asexual blood stages of Plasmodium falciparum by a granule exocytosis-dependent cytotoxic pathway that requires granulysin
    Salah E Farouk
    Department of Immunology, Stockholm University, Svante Arrheniusvägen 16, S 10691 Stockholm, Sweden
    Eur J Immunol 34:2248-56. 2004
    ..falciparum is based on the granule exocytosis-dependent cytotoxic pathway and that the presence of granulysin is essential to maintain efficient killing...
  44. ncbi request reprint NK cells use perforin rather than granulysin for anticryptococcal activity
    Ling Ling Ma
    Department of Medical Sciences, University of Calgary, Alberta, Canada
    J Immunol 173:3357-65. 2004
    ....
  45. ncbi request reprint A novel apoptosis pathway activated by the carboxyl terminus of p21
    Chen Dong
    Division of Immunology and Transplantation Biology, Department of Pediatrics, CCSR 2105, 300 Pasteur Dr, Stanford University, Stanford, CA 94305 5164, USA
    Blood 105:1187-94. 2005
    ..Targeting the p21 peptide to specific cell populations may allow development of novel therapies to eliminate aberrant cells in human diseases...
  46. ncbi request reprint Lesional T cells and dermal dendrocytes in psoriasis plaque express increased levels of granulysin
    Siba P Raychaudhuri
    Psoriasis Research Institute, Palo Alto and Stanford University School of Medicine, Palo Alto, California, USA
    J Am Acad Dermatol 51:1006-8. 2004
    ..Increased levels of granulysin provide an explanation for relative immunity of psoriatic plaques against both gram-positive and gram-negative bacterial infections...
  47. ncbi request reprint DQ 65-79, a peptide derived from HLA class II, induces I kappa B expression
    Yun Jiang
    Department of Cardiothoracic Surgery, Stanford University, Stanford, CA 94305 5164, USA
    J Immunol 168:3323-8. 2002
    ..These findings indicate that the DQ 65-79 peptide increases the level of IkappaB proteins, thereby preventing nuclear translocation of the transcription factor, NF-kappaB, and inhibiting T cell proliferation...

Research Grants2

  1. Granulysin: A Novel Cytolytic Molecule
    David Lewis; Fiscal Year: 2006
    ..The ultimate goal of these studies is to use granulysin, peptide components, and/or the information gained from the mechanistic studies to develop new antibiotics and/or tumor chemotherapeutic agents. ..