- A rapid, cost-effective method of assembly and purification of synthetic DNA probes >100 bpMichael A Jensen
Stanford Genome Technology Center, Palo Alto, California, United States of America
PLoS ONE 7:e34373. 2012....
- DMSO and betaine greatly improve amplification of GC-rich constructs in de novo synthesisMichael A Jensen
Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA
PLoS ONE 5:e11024. 2010..Furthermore, we believe either additive will allow for the production of a wide variety of GC-rich gene constructs without the need for expensive and time-consuming sample extraction and purification prior to downstream application...
- Gas-phase cleavage and dephosphorylation of universal linker-bound oligodeoxynucleotidesMichael A Jensen
Stanford Genome Technology Center, Stanford University, 855 S California Ave, Palo Alto, CA 94304, USA
Nucleosides Nucleotides Nucleic Acids 29:867-78. 2010..Finally, performance between the two linkers was similar enough to conclude each fulfills the desired requirements for mainstream, high-throughput oligodeoxynucleotide cleavage/deprotection and dephosphorylation in the gas phase...
- A novel catechol-based universal support for oligonucleotide synthesisKeith M Anderson
Stanford Genome Technology Center, Stanford University, Palo Alto, California 94304, USA
J Org Chem 72:9875-80. 2007..The flexibility of the universal support and the efficiency of 3'-dephosphorylation are expected to increase the use of universal supports in oligonucleotide synthesis...