Leonore Herzenberg

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. ncbi request reprint Epitope-specific regulation: the elephant in the bathtub
    Leonore A Herzenberg
    Department of Genetics, Stanford University, Stanford, California 94305, USA
    Nat Immunol 8:783-6. 2007
  2. ncbi request reprint Interpreting flow cytometry data: a guide for the perplexed
    Leonore A Herzenberg
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305 5318, USA
    Nat Immunol 7:681-5. 2006
  3. ncbi request reprint Genetics, FACS, immunology, and redox: a tale of two lives intertwined
    Leonard A Herzenberg
    Genetics Department, Stanford University Medical School, Beckman Center, Stanford, California 94305 5318, USA
    Annu Rev Immunol 22:1-31. 2004
  4. pmc Profound functional and signaling changes in viable inflammatory neutrophils homing to cystic fibrosis airways
    Rabindra Tirouvanziam
    Departments of Pediatrics, Genetics, and Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 105:4335-9. 2008
  5. pmc High-dose oral N-acetylcysteine, a glutathione prodrug, modulates inflammation in cystic fibrosis
    Rabindra Tirouvanziam
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 103:4628-33. 2006
  6. ncbi request reprint Protein glutathionylation: coupling and uncoupling of glutathione to protein thiol groups in lymphocytes under oxidative stress and HIV infection
    Pietro Ghezzi
    Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
    Mol Immunol 38:773-80. 2002
  7. ncbi request reprint Photogenerated glycan arrays identify immunogenic sugar moieties of Bacillus anthracis exosporium
    Denong Wang
    Carbohydrate Microarray Laboratory, Stanford University School of Medicine, Stanford, CA, USA
    Proteomics 7:180-4. 2007
  8. ncbi request reprint N-Acetylcysteine--a safe antidote for cysteine/glutathione deficiency
    Kondala R Atkuri
    Department of Genetics, School of Medicine, Stanford University, B007 Beckman Center, 279 Campus Drive, Stanford University, CA 94305 5318, United States
    Curr Opin Pharmacol 7:355-9. 2007
  9. pmc Importance of culturing primary lymphocytes at physiological oxygen levels
    Kondala R Atkuri
    Departments of Genetics and Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 104:4547-52. 2007
  10. pmc Culturing at atmospheric oxygen levels impacts lymphocyte function
    Kondala R Atkuri
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 102:3756-9. 2005

Research Grants

  1. Automated Analysis: Hi-D FACS data by Scale-Space Method
    Leonore Herzenberg; Fiscal Year: 2003
  2. Compensation Automation for HIV Vaccine Development
    Leonore A Herzenberg; Fiscal Year: 2010
  3. Compensation Automation for HIV Vaccine Development
    Leonore A Herzenberg; Fiscal Year: 2010
  4. New Perspective on Innate Antibodies in Mice
    Leonore A Herzenberg; Fiscal Year: 2010
  5. Compensation Automation for HIV Vaccine Development
    Leonore Herzenberg; Fiscal Year: 2009
  6. Automating FACS data analysis for HIV studies
    Leonore Herzenberg; Fiscal Year: 2007
  7. Do antibodies to HIV-Tat regulate redox status in HIV?
    Leonore Herzenberg; Fiscal Year: 2007
  8. Bioluminescence imaging to monitor T cell localization
    Leonore Herzenberg; Fiscal Year: 2006
  9. Sensitivity of TCR signaling to GHS loss in HIV disease
    Leonore Herzenberg; Fiscal Year: 2005
  10. Human Lymphocyte Function in Air or at Physiologic O2
    Leonore Herzenberg; Fiscal Year: 2004

Collaborators

Detail Information

Publications29

  1. ncbi request reprint Epitope-specific regulation: the elephant in the bathtub
    Leonore A Herzenberg
    Department of Genetics, Stanford University, Stanford, California 94305, USA
    Nat Immunol 8:783-6. 2007
  2. ncbi request reprint Interpreting flow cytometry data: a guide for the perplexed
    Leonore A Herzenberg
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305 5318, USA
    Nat Immunol 7:681-5. 2006
  3. ncbi request reprint Genetics, FACS, immunology, and redox: a tale of two lives intertwined
    Leonard A Herzenberg
    Genetics Department, Stanford University Medical School, Beckman Center, Stanford, California 94305 5318, USA
    Annu Rev Immunol 22:1-31. 2004
  4. pmc Profound functional and signaling changes in viable inflammatory neutrophils homing to cystic fibrosis airways
    Rabindra Tirouvanziam
    Departments of Pediatrics, Genetics, and Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 105:4335-9. 2008
    ..Thus, we identified a subset of neutrophils within CF airways with a viable but dysfunctional phenotype. This subset provides a possible therapeutic target and indicates a need to revisit current paradigms of CF airway disease...
  5. pmc High-dose oral N-acetylcysteine, a glutathione prodrug, modulates inflammation in cystic fibrosis
    Rabindra Tirouvanziam
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 103:4628-33. 2006
    ..032). Thus, high-dose oral N-acetylcysteine has the potential to counter the intertwined redox and inflammatory imbalances in CF...
  6. ncbi request reprint Protein glutathionylation: coupling and uncoupling of glutathione to protein thiol groups in lymphocytes under oxidative stress and HIV infection
    Pietro Ghezzi
    Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
    Mol Immunol 38:773-80. 2002
    ..Thus, reversible glutathionylation of proteins can rapidly shift the activity of a key metabolic enzyme and thereby result in dramatic, reversible changes in cellular metabolism...
  7. ncbi request reprint Photogenerated glycan arrays identify immunogenic sugar moieties of Bacillus anthracis exosporium
    Denong Wang
    Carbohydrate Microarray Laboratory, Stanford University School of Medicine, Stanford, CA, USA
    Proteomics 7:180-4. 2007
    ..anthracis exosporium. Since this sugar moiety is highly specific for the spores of B. anthracis, it appears to be a key biomarker for detection of B. anthracis spores and development of novel vaccines that target anthrax spores...
  8. ncbi request reprint N-Acetylcysteine--a safe antidote for cysteine/glutathione deficiency
    Kondala R Atkuri
    Department of Genetics, School of Medicine, Stanford University, B007 Beckman Center, 279 Campus Drive, Stanford University, CA 94305 5318, United States
    Curr Opin Pharmacol 7:355-9. 2007
    ....
  9. pmc Importance of culturing primary lymphocytes at physiological oxygen levels
    Kondala R Atkuri
    Departments of Genetics and Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 104:4547-52. 2007
    ..Thus, we suggest caution in culturing primary lymphocytes at atmosO(2) because the requisite adaptation to nonphysiological oxygen levels may seriously skew T cell responses, particularly after several days in culture...
  10. pmc Culturing at atmospheric oxygen levels impacts lymphocyte function
    Kondala R Atkuri
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 102:3756-9. 2005
    ....
  11. ncbi request reprint The extracellular microenvironment plays a key role in regulating the redox status of cell surface proteins in HIV-infected subjects
    Bita Sahaf
    Herzenberg Laboratory, Department of Genetics, Beckman Center, B 007 Stanford University School of Medicine Stanford, CA 94304 5318, USA
    Arch Biochem Biophys 434:26-32. 2005
    ....
  12. doi request reprint FR255734, a humanized, Fc-Silent, Anti-CD28 antibody, improves psoriasis in the SCID mouse-psoriasis xenograft model
    Siba P Raychaudhuri
    Department of Genetics, Stanford University School of Medicine, Stanford, California, USA
    J Invest Dermatol 128:1969-76. 2008
    ..In vitro studies have shown that FR255734 effectively blocked T-cell proliferation and proinflammatory cytokine production. These observations warrant studies to evaluate the efficacy of FR255734 in human autoimmune diseases...
  13. pmc Motexafin gadolinium (Gd-Tex) selectively induces apoptosis in HIV-1 infected CD4+ T helper cells
    Omar D Perez
    Department of Microbiology and Immunology, The Baxter Laboratories of Genetic Pharmacology, Stanford University, Stanford, CA 94305 5175, USA
    Proc Natl Acad Sci U S A 99:2270-4. 2002
    ..These findings suggest that Gd-Tex may have therapeutic utility as an anti-HIV agent capable of selectively targeting and removing HIV-infected cells in an infected host...
  14. pmc Culturing of human peripheral blood cells reveals unsuspected lymphocyte responses relevant to HIV disease
    Bita Sahaf
    Department of Genetics and Department of Pediatrics, Division of Stem Cell Transplantation, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 105:5111-6. 2008
    ..These findings strongly recommend culturing primary cells at physiological oxygen levels. In addition, they suggest HIV-Tat as a key regulator of HIV disease progression...
  15. ncbi request reprint The history and future of the fluorescence activated cell sorter and flow cytometry: a view from Stanford
    Leonard A Herzenberg
    Stanford University, Department of Genetics, Stanford, CA 94305, USA
    Clin Chem 48:1819-27. 2002
    ..Thus, key functions can be studied in various subsets of cells without the need for prior sorting...
  16. pmc B cell-dependent T cell responses: IgM antibodies are required to elicit contact sensitivity
    Ryohei F Tsuji
    Noda Institute for Scientific Research, Chiba ken 278, Japan
    J Exp Med 196:1277-90. 2002
    ..The IgM and challenge antigen likely form local complexes that activate complement, generating C5a, leading to local vascular activation to recruit the antigen-primed effector T cells that mediate the CS response...
  17. pmc Lymphocyte surface thiol levels
    Bita Sahaf
    Department of Genetics, Stanford School of Medicine, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 100:4001-5. 2003
    ..Collectively, our findings indicate that local environment plays a greater role in determining the redox status of cell surface molecules than the internal redox status of the cells...
  18. ncbi request reprint Ontogeny of gamma delta T cells in humans
    Stephen C De Rosa
    Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 172:1637-45. 2004
    ..This represents the earliest immunological maturation of any lymphocyte compartment in humans and most likely indicates the importance of these cells in controlling pathology due to common environmental challenges...
  19. ncbi request reprint New approaches to fluorescence compensation and visualization of FACS data
    James W Tung
    Department of Genetics, Stanford University Medical School, Stanford, CA 94305 5120, USA
    Clin Immunol 110:277-83. 2004
    ..Finally, we discuss controls that facilitate recognition of boundaries between positive and negative subsets...
  20. pmc Division and differentiation of natural antibody-producing cells in mouse spleen
    Yang Yang
    Department of Genetics, Stanford University, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 104:4542-6. 2007
    ..Thus, the studies present a different view of the roles that B-1a cells play in the early phases of the innate immune response...
  21. pmc Modern flow cytometry: a practical approach
    James W Tung
    Department of Genetics, School of Medicine, Stanford University, Stanford, CA 94305, USA
    Clin Lab Med 27:453-68, v. 2007
    ..FACS technology development and the emergence of new software support for this technology are cooperating in this effort...
  22. ncbi request reprint Unraveling B-1 progenitors
    James W Tung
    Department of Genetics, Stanford School of Medicine, Stanford University, Stanford, CA 94305, USA
    Curr Opin Immunol 19:150-5. 2007
    ..Notably, the identification of CD45R(-/lo)CD19(+) B-1 progenitors and expression of two surface determinants, CD138 and major histocompatibility class II antigens, distinguish developing B-1 cells from B-2 cells...
  23. pmc B-cell development fails in the absence of the Pbx1 proto-oncogene
    Mrinmoy Sanyal
    Department of Pathology, Stanford University, School of Medicine, Stanford, CA 94305, USA
    Blood 109:4191-9. 2007
    ..Thus, Pbx1 critically functions at a stage between hematopoietic stem cell development and B-cell commitment and, therefore, is one of the earliest-acting transcription factors that regulate de novo B-lineage lymphopoiesis...
  24. ncbi request reprint MYC can induce DNA breaks in vivo and in vitro independent of reactive oxygen species
    Suma Ray
    Division of Oncology, Department of Medicine and Pathology, Stanford University School of Medicine, 269 Campus Drive, Stanford, CA 94305, USA
    Cancer Res 66:6598-605. 2006
    ..Hence, MYC overexpression can induce ROS and SSBs under some conditions, but generally induces widespread DSBs in vivo and in vitro independent of ROS production...
  25. pmc Phenotypically distinct B cell development pathways map to the three B cell lineages in the mouse
    James W Tung
    Department of Genetics, Stanford School of Medicine, Stanford University, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 103:6293-8. 2006
    ..Thus, CD138 expression provides a key route to distinguishing early B cell development pathway for what now are clearly three B cell lineages...
  26. ncbi request reprint B cell lineages: documented at last!
    Leonore A Herzenberg
    Nat Immunol 7:225-6. 2006
  27. ncbi request reprint Identification of B-cell subsets: an exposition of 11-color (Hi-D) FACS methods
    James W Tung
    Department of Genetics, Stanford University Medical School, Stanford, CA, USA
    Methods Mol Biol 271:37-58. 2004
    ..In addition, it facilitates discrimination of valid subsets. Application of Logicle visualization tools in the Hi-D FACS studies discussed here creates a strong new base for in-depth analysis of B-cell development and function...
  28. pmc Identification of a germ-line pro-B cell subset that distinguishes the fetal/neonatal from the adult B cell development pathway
    Li Sheng Lu
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 99:3007-12. 2002
    ....
  29. pmc CD11b expression distinguishes sequential stages of peritoneal B-1 development
    Eliver Eid Bou Ghosn
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 105:5195-200. 2008
    ..However, the clear phenotypic differences between the neonatal and adult CD11b B-1 subsets argue that although CD11b(-) B-1 give rise to CD11b(+) B-1 in both cases different forces may regulate this transition...

Research Grants14

  1. Automated Analysis: Hi-D FACS data by Scale-Space Method
    Leonore Herzenberg; Fiscal Year: 2003
    ..When the techniques are working well, we will transform them into a form that can be made available to FACS users generally. ..
  2. Compensation Automation for HIV Vaccine Development
    Leonore A Herzenberg; Fiscal Year: 2010
    ..Basically, at the close of the data preparation phase, the data should be ready for analysis and the investigator should either be confident that the processed data is reliable or know why it is not! ..
  3. Compensation Automation for HIV Vaccine Development
    Leonore A Herzenberg; Fiscal Year: 2010
    ..Basically, at the close of the data preparation phase, the data should be ready for analysis and the investigator should either be confident that the processed data is reliable or know why it is not! ..
  4. New Perspective on Innate Antibodies in Mice
    Leonore A Herzenberg; Fiscal Year: 2010
    ....
  5. Compensation Automation for HIV Vaccine Development
    Leonore Herzenberg; Fiscal Year: 2009
    ..Basically, at the close of the data preparation phase, the data should be ready for analysis and the investigator should either be confident that the processed data is reliable or know why it is not! ..
  6. Automating FACS data analysis for HIV studies
    Leonore Herzenberg; Fiscal Year: 2007
    ....
  7. Do antibodies to HIV-Tat regulate redox status in HIV?
    Leonore Herzenberg; Fiscal Year: 2007
    ..Collectively, these studies will contribute to understanding the source(s) of oxidative stress in HIV infection and to identifying potential markers for predicting HIV disease progression. ..
  8. Bioluminescence imaging to monitor T cell localization
    Leonore Herzenberg; Fiscal Year: 2006
    ..Development of these transgenic mice enables the biological studies defined in this project and biological studies other laboratories. ..
  9. Sensitivity of TCR signaling to GHS loss in HIV disease
    Leonore Herzenberg; Fiscal Year: 2005
    ..Finally, the technology we develop here will provide new and better ways to monitor disease progress and evaluate therapeutic interventions. ..
  10. Human Lymphocyte Function in Air or at Physiologic O2
    Leonore Herzenberg; Fiscal Year: 2004
    ....
  11. New Perspective on Innate Antibodies in Mice
    Leonore A Herzenberg; Fiscal Year: 2010
    ....