Leonore A Herzenberg

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. ncbi request reprint The history and future of the fluorescence activated cell sorter and flow cytometry: a view from Stanford
    Leonard A Herzenberg
    Stanford University, Department of Genetics, Stanford, CA 94305, USA
    Clin Chem 48:1819-27. 2002
  2. ncbi request reprint Genetics, FACS, immunology, and redox: a tale of two lives intertwined
    Leonard A Herzenberg
    Genetics Department, Stanford University Medical School, Beckman Center, Stanford, California 94305 5318, USA
    Annu Rev Immunol 22:1-31. 2004
  3. pmc Antigen-specific memory in B-1a and its relationship to natural immunity
    Yang Yang
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 109:5388-93. 2012
  4. pmc Two physically, functionally, and developmentally distinct peritoneal macrophage subsets
    Eliver Eid Bou Ghosn
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 107:2568-73. 2010
  5. doi request reprint Blood basophils from cystic fibrosis patients with allergic bronchopulmonary aspergillosis are primed and hyper-responsive to stimulation by aspergillus allergens
    Yael Gernez
    Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
    J Cyst Fibros 11:502-10. 2012
  6. pmc Importance of culturing primary lymphocytes at physiological oxygen levels
    Kondala R Atkuri
    Departments of Genetics and Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 104:4547-52. 2007
  7. pmc Division and differentiation of natural antibody-producing cells in mouse spleen
    Yang Yang
    Department of Genetics, Stanford University, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 104:4542-6. 2007
  8. pmc Profound functional and signaling changes in viable inflammatory neutrophils homing to cystic fibrosis airways
    Rabindra Tirouvanziam
    Departments of Pediatrics, Genetics, and Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 105:4335-9. 2008
  9. pmc Antigen-specific antibody responses in B-1a and their relationship to natural immunity
    Yang Yang
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 109:5382-7. 2012
  10. ncbi request reprint Identification of B-cell subsets: an exposition of 11-color (Hi-D) FACS methods
    James W Tung
    Department of Genetics, Stanford University Medical School, Stanford, CA, USA
    Methods Mol Biol 271:37-58. 2004

Collaborators

Detail Information

Publications48

  1. ncbi request reprint The history and future of the fluorescence activated cell sorter and flow cytometry: a view from Stanford
    Leonard A Herzenberg
    Stanford University, Department of Genetics, Stanford, CA 94305, USA
    Clin Chem 48:1819-27. 2002
    ..Thus, key functions can be studied in various subsets of cells without the need for prior sorting...
  2. ncbi request reprint Genetics, FACS, immunology, and redox: a tale of two lives intertwined
    Leonard A Herzenberg
    Genetics Department, Stanford University Medical School, Beckman Center, Stanford, California 94305 5318, USA
    Annu Rev Immunol 22:1-31. 2004
  3. pmc Antigen-specific memory in B-1a and its relationship to natural immunity
    Yang Yang
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 109:5388-93. 2012
    ..In addition, at a practical level, these studies suggest previously unexplored vaccination strategies for pathogen-associated antigens that target the B-1a repertoire...
  4. pmc Two physically, functionally, and developmentally distinct peritoneal macrophage subsets
    Eliver Eid Bou Ghosn
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 107:2568-73. 2010
    ..Thus, they introduce a new context for interpreting (and reinterpreting) data from ex vivo studies with PerC MØ...
  5. doi request reprint Blood basophils from cystic fibrosis patients with allergic bronchopulmonary aspergillosis are primed and hyper-responsive to stimulation by aspergillus allergens
    Yael Gernez
    Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
    J Cyst Fibros 11:502-10. 2012
    ..Since basophils play a key role in allergy, we hypothesized that they would display alterations in CF-ABPA patients compared to CF-AC or patients without Af colonization (CF)...
  6. pmc Importance of culturing primary lymphocytes at physiological oxygen levels
    Kondala R Atkuri
    Departments of Genetics and Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 104:4547-52. 2007
    ..Thus, we suggest caution in culturing primary lymphocytes at atmosO(2) because the requisite adaptation to nonphysiological oxygen levels may seriously skew T cell responses, particularly after several days in culture...
  7. pmc Division and differentiation of natural antibody-producing cells in mouse spleen
    Yang Yang
    Department of Genetics, Stanford University, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 104:4542-6. 2007
    ..Thus, the studies present a different view of the roles that B-1a cells play in the early phases of the innate immune response...
  8. pmc Profound functional and signaling changes in viable inflammatory neutrophils homing to cystic fibrosis airways
    Rabindra Tirouvanziam
    Departments of Pediatrics, Genetics, and Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 105:4335-9. 2008
    ..Thus, we identified a subset of neutrophils within CF airways with a viable but dysfunctional phenotype. This subset provides a possible therapeutic target and indicates a need to revisit current paradigms of CF airway disease...
  9. pmc Antigen-specific antibody responses in B-1a and their relationship to natural immunity
    Yang Yang
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 109:5382-7. 2012
    ..These findings introduce previously unexplored vaccination strategies for pathogens that target the B-1a repertoire...
  10. ncbi request reprint Identification of B-cell subsets: an exposition of 11-color (Hi-D) FACS methods
    James W Tung
    Department of Genetics, Stanford University Medical School, Stanford, CA, USA
    Methods Mol Biol 271:37-58. 2004
    ..In addition, it facilitates discrimination of valid subsets. Application of Logicle visualization tools in the Hi-D FACS studies discussed here creates a strong new base for in-depth analysis of B-cell development and function...
  11. pmc Distinct progenitors for B-1 and B-2 cells are present in adult mouse spleen
    Eliver Eid Bou Ghosn
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 108:2879-84. 2011
    ..In adults, in vivo inflammatory stimulation (LPS) triggers B-1 progenitors in spleen to expand and initiate development along this B-1 developmental pathway...
  12. pmc Activation of critical, host-induced, metabolic and stress pathways marks neutrophil entry into cystic fibrosis lungs
    Megha Makam
    Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 106:5779-83. 2009
    ..Taken together, our data demonstrate an early and sustained activation of host-responsive metabolic and stress pathways upon neutrophil entry into CF airways, suggesting potential targets for therapeutic modulation...
  13. pmc Distinct B-cell lineage commitment distinguishes adult bone marrow hematopoietic stem cells
    Eliver Eid Bou Ghosn
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 109:5394-8. 2012
    ..In addition, of importance to contemporary medicine, we consider the implications that HSC developmental heterogeneity may have for selecting HSC sources for human transplantation...
  14. doi request reprint Modulation of mTOR effector phosphoproteins in blood basophils from allergic patients
    Yael Gernez
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Clin Immunol 32:565-73. 2012
    ..Thus, basophil responses to offending allergens are associated with modulation of mTOR effector phosphoproteins...
  15. pmc Phenotypically distinct B cell development pathways map to the three B cell lineages in the mouse
    James W Tung
    Department of Genetics, Stanford School of Medicine, Stanford University, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 103:6293-8. 2006
    ..Thus, CD138 expression provides a key route to distinguishing early B cell development pathway for what now are clearly three B cell lineages...
  16. pmc Culturing of human peripheral blood cells reveals unsuspected lymphocyte responses relevant to HIV disease
    Bita Sahaf
    Department of Genetics and Department of Pediatrics, Division of Stem Cell Transplantation, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 105:5111-6. 2008
    ..These findings strongly recommend culturing primary cells at physiological oxygen levels. In addition, they suggest HIV-Tat as a key regulator of HIV disease progression...
  17. ncbi request reprint The extracellular microenvironment plays a key role in regulating the redox status of cell surface proteins in HIV-infected subjects
    Bita Sahaf
    Herzenberg Laboratory, Department of Genetics, Beckman Center, B 007 Stanford University School of Medicine Stanford, CA 94304 5318, USA
    Arch Biochem Biophys 434:26-32. 2005
    ....
  18. pmc Reversal of paralysis and reduced inflammation from peripheral administration of β-amyloid in TH1 and TH17 versions of experimental autoimmune encephalomyelitis
    Jacqueline L Grant
    Department of Neurology and Neurological Sciences, Stanford University, Stanford, CA 94305, USA
    Sci Transl Med 4:145ra105. 2012
    ..Because Aβ42 and Aβ40 ameliorate experimental autoimmune inflammation targeting the CNS, we might now consider its potential anti-inflammatory role in other neuropathological conditions...
  19. pmc Basophil CD203c levels are increased at baseline and can be used to monitor omalizumab treatment in subjects with nut allergy
    Yael Gernez
    Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Int Arch Allergy Immunol 154:318-27. 2011
    ..Basophils contribute to anaphylaxis and allergies. We examined the utility of assessing basophil-associated surface antigens (CD11b/CD63/CD123/CD203c/CD294) in characterizing and monitoring subjects with nut allergy...
  20. ncbi request reprint N-Acetylcysteine--a safe antidote for cysteine/glutathione deficiency
    Kondala R Atkuri
    Department of Genetics, School of Medicine, Stanford University, B007 Beckman Center, 279 Campus Drive, Stanford University, CA 94305 5318, United States
    Curr Opin Pharmacol 7:355-9. 2007
    ....
  21. pmc H-Y antigen-binding B cells develop in male recipients of female hematopoietic cells and associate with chronic graft vs. host disease
    Bita Sahaf
    Division of Blood and Marrow Transplantation, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 110:3005-10. 2013
    ..Our study suggests that detection of anti-DBY-2 B cells may predict cGVHD and that this prediction may have clinical utility. Validation of this hypothesis will require larger prospective studies...
  22. pmc Modern flow cytometry: a practical approach
    James W Tung
    Department of Genetics, School of Medicine, Stanford University, Stanford, CA 94305, USA
    Clin Lab Med 27:453-68, v. 2007
    ..FACS technology development and the emergence of new software support for this technology are cooperating in this effort...
  23. pmc CD11b expression distinguishes sequential stages of peritoneal B-1 development
    Eliver Eid Bou Ghosn
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 105:5195-200. 2008
    ..However, the clear phenotypic differences between the neonatal and adult CD11b B-1 subsets argue that although CD11b(-) B-1 give rise to CD11b(+) B-1 in both cases different forces may regulate this transition...
  24. pmc B-cell development fails in the absence of the Pbx1 proto-oncogene
    Mrinmoy Sanyal
    Department of Pathology, Stanford University, School of Medicine, Stanford, CA 94305, USA
    Blood 109:4191-9. 2007
    ..Thus, Pbx1 critically functions at a stage between hematopoietic stem cell development and B-cell commitment and, therefore, is one of the earliest-acting transcription factors that regulate de novo B-lineage lymphopoiesis...
  25. ncbi request reprint New approaches to fluorescence compensation and visualization of FACS data
    James W Tung
    Department of Genetics, Stanford University Medical School, Stanford, CA 94305 5120, USA
    Clin Immunol 110:277-83. 2004
    ..Finally, we discuss controls that facilitate recognition of boundaries between positive and negative subsets...
  26. ncbi request reprint Interpreting flow cytometry data: a guide for the perplexed
    Leonore A Herzenberg
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305 5318, USA
    Nat Immunol 7:681-5. 2006
  27. ncbi request reprint Protein glutathionylation: coupling and uncoupling of glutathione to protein thiol groups in lymphocytes under oxidative stress and HIV infection
    Pietro Ghezzi
    Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
    Mol Immunol 38:773-80. 2002
    ..Thus, reversible glutathionylation of proteins can rapidly shift the activity of a key metabolic enzyme and thereby result in dramatic, reversible changes in cellular metabolism...
  28. doi request reprint A randomized controlled pilot trial of oral N-acetylcysteine in children with autism
    Antonio Y Hardan
    Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, California 94305, USA
    Biol Psychiatry 71:956-61. 2012
    ..The goal of this pilot study was to assess the feasibility of using oral N-acetylcysteine (NAC), a glutamatergic modulator and an antioxidant, in the treatment of behavioral disturbance in children with autism...
  29. doi request reprint Distinct plasma profile of polar neutral amino acids, leucine, and glutamate in children with Autism Spectrum Disorders
    Rabindra Tirouvanziam
    Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Autism Dev Disord 42:827-36. 2012
    ..Findings from this limited prospective study warrant further examination of plasma AA levels in larger cross-sectional and longitudinal cohorts to adequately assess for relationships with developmental and clinical features of ASD...
  30. pmc Lymphocyte surface thiol levels
    Bita Sahaf
    Department of Genetics, Stanford School of Medicine, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 100:4001-5. 2003
    ..Collectively, our findings indicate that local environment plays a greater role in determining the redox status of cell surface molecules than the internal redox status of the cells...
  31. ncbi request reprint Unraveling B-1 progenitors
    James W Tung
    Department of Genetics, Stanford School of Medicine, Stanford University, Stanford, CA 94305, USA
    Curr Opin Immunol 19:150-5. 2007
    ..Notably, the identification of CD45R(-/lo)CD19(+) B-1 progenitors and expression of two surface determinants, CD138 and major histocompatibility class II antigens, distinguish developing B-1 cells from B-2 cells...
  32. pmc Inherited disorders affecting mitochondrial function are associated with glutathione deficiency and hypocitrullinemia
    Kondala R Atkuri
    Department of Genetics, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 106:3941-5. 2009
    ..Furthermore, studies here suggest that antioxidant therapy may be useful for relieving the chronic oxidative stress that otherwise occurs in patients with mitochondrial dysfunction...
  33. ncbi request reprint Epitope-specific regulation: the elephant in the bathtub
    Leonore A Herzenberg
    Department of Genetics, Stanford University, Stanford, California 94305, USA
    Nat Immunol 8:783-6. 2007
  34. doi request reprint FR255734, a humanized, Fc-Silent, Anti-CD28 antibody, improves psoriasis in the SCID mouse-psoriasis xenograft model
    Siba P Raychaudhuri
    Department of Genetics, Stanford University School of Medicine, Stanford, California, USA
    J Invest Dermatol 128:1969-76. 2008
    ..In vitro studies have shown that FR255734 effectively blocked T-cell proliferation and proinflammatory cytokine production. These observations warrant studies to evaluate the efficacy of FR255734 in human autoimmune diseases...
  35. ncbi request reprint Molecular imaging using labeled donor tissues reveals patterns of engraftment, rejection, and survival in transplantation
    Yu An Cao
    Department of Pediatrics, Stanford School of Medicine, Stanford, California, USA
    Transplantation 80:134-9. 2005
    ..This imaging approach is sensitive and reproducible, permits study of the dynamic range of the entire process of transplantation, and will greatly enhance studies across various disciplines involving transplantation...
  36. pmc Culturing at atmospheric oxygen levels impacts lymphocyte function
    Kondala R Atkuri
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 102:3756-9. 2005
    ....
  37. ncbi request reprint MYC can induce DNA breaks in vivo and in vitro independent of reactive oxygen species
    Suma Ray
    Division of Oncology, Department of Medicine and Pathology, Stanford University School of Medicine, 269 Campus Drive, Stanford, CA 94305, USA
    Cancer Res 66:6598-605. 2006
    ..Hence, MYC overexpression can induce ROS and SSBs under some conditions, but generally induces widespread DSBs in vivo and in vitro independent of ROS production...
  38. pmc High-dose oral N-acetylcysteine, a glutathione prodrug, modulates inflammation in cystic fibrosis
    Rabindra Tirouvanziam
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 103:4628-33. 2006
    ..032). Thus, high-dose oral N-acetylcysteine has the potential to counter the intertwined redox and inflammatory imbalances in CF...
  39. doi request reprint A conversation with Leonard and Leonore Herzenberg
    Leonard A Herzenberg
    Department of Genetics, Stanford University, Stanford, California 94305
    Annu Rev Physiol 76:1-20. 2014
    ..Their unique philosophy has shaped their lives, their science, and ultimately the scientific community. In this Conversation, we explore some of these driving forces and the impact on the laboratory. ..
  40. ncbi request reprint AutoGate: automating analysis of flow cytometry data
    Stephen Meehan
    Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94305, USA
    Immunol Res 58:218-23. 2014
    ..When this "early adopter" phase is complete, the authors expect to distribute the software free of charge to .edu, .org and .gov users...
  41. ncbi request reprint Selective uptake of single-walled carbon nanotubes by circulating monocytes for enhanced tumour delivery
    Bryan Ronain Smith
    1 Molecular Imaging Program at Stanford, The James H Clark Center, Stanford University, Stanford, California 94305, USA 2 Department of Radiology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nat Nanotechnol 9:481-7. 2014
    ..001, day 7 post-injection). The remarkable selectivity of this tumour-targeting mechanism demonstrates an advanced immune-based delivery strategy for enhancing specific tumour delivery with substantial penetration. ..
  42. ncbi request reprint Photogenerated glycan arrays identify immunogenic sugar moieties of Bacillus anthracis exosporium
    Denong Wang
    Carbohydrate Microarray Laboratory, Stanford University School of Medicine, Stanford, CA, USA
    Proteomics 7:180-4. 2007
    ..anthracis exosporium. Since this sugar moiety is highly specific for the spores of B. anthracis, it appears to be a key biomarker for detection of B. anthracis spores and development of novel vaccines that target anthrax spores...
  43. pmc Identification of a germ-line pro-B cell subset that distinguishes the fetal/neonatal from the adult B cell development pathway
    Li Sheng Lu
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 99:3007-12. 2002
    ....
  44. pmc Motexafin gadolinium (Gd-Tex) selectively induces apoptosis in HIV-1 infected CD4+ T helper cells
    Omar D Perez
    Department of Microbiology and Immunology, The Baxter Laboratories of Genetic Pharmacology, Stanford University, Stanford, CA 94305 5175, USA
    Proc Natl Acad Sci U S A 99:2270-4. 2002
    ..These findings suggest that Gd-Tex may have therapeutic utility as an anti-HIV agent capable of selectively targeting and removing HIV-infected cells in an infected host...
  45. pmc The E47 transcription factor negatively regulates CD5 expression during thymocyte development
    Yang Yang
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 101:3898-902. 2004
    ....
  46. pmc B cell-dependent T cell responses: IgM antibodies are required to elicit contact sensitivity
    Ryohei F Tsuji
    Noda Institute for Scientific Research, Chiba ken 278, Japan
    J Exp Med 196:1277-90. 2002
    ..The IgM and challenge antigen likely form local complexes that activate complement, generating C5a, leading to local vascular activation to recruit the antigen-primed effector T cells that mediate the CS response...
  47. ncbi request reprint Ontogeny of gamma delta T cells in humans
    Stephen C De Rosa
    Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 172:1637-45. 2004
    ..This represents the earliest immunological maturation of any lymphocyte compartment in humans and most likely indicates the importance of these cells in controlling pathology due to common environmental challenges...
  48. ncbi request reprint B cell lineages: documented at last!
    Leonore A Herzenberg
    Nat Immunol 7:225-6. 2006