Simon Fredriksson

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. doi request reprint Multiplexed proximity ligation assays to profile putative plasma biomarkers relevant to pancreatic and ovarian cancer
    Simon Fredriksson
    Stanford Genome Technology Center, Bio X, Stanford University, Stanford, CA, USA
    Clin Chem 54:582-9. 2008
  2. ncbi request reprint Multiplexed protein detection by proximity ligation for cancer biomarker validation
    Simon Fredriksson
    Stanford Genome Technology Center, Stanford, California 94305, USA
    Nat Methods 4:327-9. 2007
  3. pmc Multiplex amplification of all coding sequences within 10 cancer genes by Gene-Collector
    Simon Fredriksson
    Stanford Genome Technology Center, Bio X, Stanford, California 94305, USA
    Nucleic Acids Res 35:e47. 2007
  4. pmc Multigene amplification and massively parallel sequencing for cancer mutation discovery
    Fredrik Dahl
    Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA
    Proc Natl Acad Sci U S A 104:9387-92. 2007
  5. ncbi request reprint A sensitive proximity ligation assay for active PSA
    Lei Zhu
    Department of Clinical Chemistry, Biomedicum Helsinki University Central Hospital, FI 00029 Helsinki, Finland
    Biol Chem 387:769-72. 2006
  6. ncbi request reprint Detection of individual microbial pathogens by proximity ligation
    Sigrun M Gustafsdottir
    The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden
    Clin Chem 52:1152-60. 2006
  7. ncbi request reprint Proximity ligation assays for sensitive and specific protein analyses
    Sigrun M Gustafsdottir
    Rudbeck Laboratory, Department of Genetics and Pathology, Uppsala University, SE 75185 Uppsala, Sweden
    Anal Biochem 345:2-9. 2005
  8. pmc Cytokine detection by antibody-based proximity ligation
    Mats Gullberg
    The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE 75185 Uppsala, Sweden
    Proc Natl Acad Sci U S A 101:8420-4. 2004
  9. ncbi request reprint A sense of closeness: protein detection by proximity ligation
    Mats Gullberg
    The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, SE 751 85 Uppsala, Sweden
    Curr Opin Biotechnol 14:82-6. 2003
  10. ncbi request reprint Protein detection using proximity-dependent DNA ligation assays
    Simon Fredriksson
    The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, SE 75185 Uppsala, Sweden
    Nat Biotechnol 20:473-7. 2002

Collaborators

Detail Information

Publications11

  1. doi request reprint Multiplexed proximity ligation assays to profile putative plasma biomarkers relevant to pancreatic and ovarian cancer
    Simon Fredriksson
    Stanford Genome Technology Center, Bio X, Stanford University, Stanford, CA, USA
    Clin Chem 54:582-9. 2008
    ..Sensitive methods are needed for biomarker discovery and validation. We tested one promising technology, multiplex proximity ligation assay (PLA), in a pilot study profiling plasma biomarkers in pancreatic and ovarian cancer...
  2. ncbi request reprint Multiplexed protein detection by proximity ligation for cancer biomarker validation
    Simon Fredriksson
    Stanford Genome Technology Center, Stanford, California 94305, USA
    Nat Methods 4:327-9. 2007
    ..The procedure can use a single polyclonal antibody batch for each target protein, simplifying affinity-reagent creation for new biomarker candidates...
  3. pmc Multiplex amplification of all coding sequences within 10 cancer genes by Gene-Collector
    Simon Fredriksson
    Stanford Genome Technology Center, Bio X, Stanford, California 94305, USA
    Nucleic Acids Res 35:e47. 2007
    ..Gene-Collector has utility for numerous applications such as high throughput resequencing, SNP analyses, and pathogen detection...
  4. pmc Multigene amplification and massively parallel sequencing for cancer mutation discovery
    Fredrik Dahl
    Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA
    Proc Natl Acad Sci U S A 104:9387-92. 2007
    ..Seven cancer cell lines and one normal genomic DNA sample were studied with multiple mutations and polymorphisms identified among the 10 genes. Mutations and polymorphisms in the TP53 gene were confirmed by traditional sequencing...
  5. ncbi request reprint A sensitive proximity ligation assay for active PSA
    Lei Zhu
    Department of Clinical Chemistry, Biomedicum Helsinki University Central Hospital, FI 00029 Helsinki, Finland
    Biol Chem 387:769-72. 2006
    ..Our results show that a highly sensitive immunopeptidometric assay can be developed using proximity ligation. This principle should facilitate establishment of specific assays for active forms of other proteases...
  6. ncbi request reprint Detection of individual microbial pathogens by proximity ligation
    Sigrun M Gustafsdottir
    The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden
    Clin Chem 52:1152-60. 2006
    ..Nucleic acid amplification allows the detection of single infectious agents. Protein-based assays, although they provide information on ongoing infections, have substantially less detection sensitivity...
  7. ncbi request reprint Proximity ligation assays for sensitive and specific protein analyses
    Sigrun M Gustafsdottir
    Rudbeck Laboratory, Department of Genetics and Pathology, Uppsala University, SE 75185 Uppsala, Sweden
    Anal Biochem 345:2-9. 2005
  8. pmc Cytokine detection by antibody-based proximity ligation
    Mats Gullberg
    The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE 75185 Uppsala, Sweden
    Proc Natl Acad Sci U S A 101:8420-4. 2004
    ..The technique is applied to measure cytokines in a homogenous test format with femtomolar detection sensitivities in 1-microl samples, and we exemplify its utility in situations when only minute sample amounts are available...
  9. ncbi request reprint A sense of closeness: protein detection by proximity ligation
    Mats Gullberg
    The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, SE 751 85 Uppsala, Sweden
    Curr Opin Biotechnol 14:82-6. 2003
    ..Through a ligation reaction a DNA reporter sequence is created, which can be amplified. The procedure thus encodes detected proteins as specific nucleic acid sequences in what may be viewed as a reverse translation reaction...
  10. ncbi request reprint Protein detection using proximity-dependent DNA ligation assays
    Simon Fredriksson
    The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, SE 75185 Uppsala, Sweden
    Nat Biotechnol 20:473-7. 2002
    ..This proximity ligation assay detects zeptomole (40 x 10(-21) mol) amounts of the cytokine platelet-derived growth factor (PDGF) without washes or separations, and the mechanism can be generalized to other forms of protein analysis...
  11. ncbi request reprint Effect of oligonucleotide truncation on single-nucleotide distinction by solid-phase hybridization
    Magnus Jobs
    Center for Genomics and Bioinformatics, Karolinska Institute, Stockholm, Sweden
    Anal Chem 74:199-202. 2002
    ..We conclude that at optimal temperatures greater oligonucleotide quality can improve the performance of oligonucleotide hybridization microarrays...