Research Topics
Genomes and Genes
Species | Guowei FangSummaryAffiliation: Stanford University Country: USA Publications
| Collaborators
|
Detail Information
Publications
The checkpoint protein Chfr is a ligase that ubiquitinates Plk1 and inhibits Cdc2 at the G2 to M transitionDongmin Kang
Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
J Cell Biol 156:249-59. 2002..Thus, the Chfr pathway represents a novel checkpoint pathway that regulates the entry into mitosis by ubiquitin-dependent proteolysis...- Checkpoint protein BubR1 acts synergistically with Mad2 to inhibit anaphase-promoting complexGuowei Fang
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
Mol Biol Cell 13:755-66. 2002..Thus, BubR1 and Mad2 act cooperatively to prevent premature separation of sister chromatids by directly inhibiting APC... 
Anillin is a substrate of anaphase-promoting complex/cyclosome (APC/C) that controls spatial contractility of myosin during late cytokinesisWei Meng Zhao
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
J Biol Chem 280:33516-24. 2005..We concluded that anillin functions to maintain the localization of active myosin, thereby ensuring the spatial control of concerted contraction during cytokinesis...- Centromere cohesion: regulating the guardianLin Fang
Department of Biological Sciences, Lokey Chemical Biology Building, Stanford University, Stanford, CA 94305-5020, USA
Cell Res 17:664-5. 2007 - Bora and the kinase Aurora a cooperatively activate the kinase Plk1 and control mitotic entryAkiko Seki
Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
Science 320:1655-8. 2008..Thus, Bora and Aur-A control mitotic entry, which provides a mechanism for one of the most important yet ill-defined events in the cell cycle... - Plk1 and Aurora A regulate the depolymerase activity and the cellular localization of Kif2aChang Young Jang
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
J Cell Sci 122:1334-41. 2009.... - A Xenopus cell-free system for analysis of the Chfr ubiquitin ligase involved in control of mitotic entryDongmin Kang
Department of Biological Sciences, Stanford University, CA, USA
Methods Mol Biol 280:229-43. 2004.... - DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movementChang Young Jang
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
J Cell Biol 181:255-67. 2008..Thus, DDA3 represents a new class of MT-destabilizing protein that controls spindle dynamics and mitotic progression by regulating MT depolymerases... - Cdk1 phosphorylation of BubR1 controls spindle checkpoint arrest and Plk1-mediated formation of the 3F3/2 epitopeOi Kwan Wong
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
J Cell Biol 179:611-7. 2007..Thus, Cdk1-mediated phosphorylation of BubR1 controls checkpoint arrest and promotes the formation of the kinetochore 3F3/2 epitope... - FAM29A, a target of Plk1 regulation, controls the partitioning of NEDD1 between the mitotic spindle and the centrosomesHui Zhu
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
J Cell Sci 122:2750-9. 2009..FAM29A controls the relative contributions of these two pathways to microtubule polymerization during mitosis... - Cep55, a microtubule-bundling protein, associates with centralspindlin to control the midbody integrity and cell abscission during cytokinesisWei Meng Zhao
Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
Mol Biol Cell 17:3881-96. 2006..Our study defines a cellular mechanism that links centralspindlin to Cep55, which, in turn, controls the midbody structure and membrane fusion at the terminal stage of cytokinesis... - FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindleHui Zhu
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
J Cell Biol 183:835-48. 2008..Our study provides a biochemical mechanism for MT-dependent MT amplification and for the maturation of kinetochore fibers in mammalian cells... - Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule-binding domainJim Wong
Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
Mol Biol Cell 19:2083-91. 2008..We concluded that phosphorylation of HURP by Aurora A provides a regulatory mechanism for the control of spindle assembly and function... - SKAP associates with kinetochores and promotes the metaphase-to-anaphase transitionLin Fang
Department of Biological Sciences, Stanford University, Stanford, CA, USA
Cell Cycle 8:2819-27. 2009.... - Plk1- and beta-TrCP-dependent degradation of Bora controls mitotic progressionAkiko Seki
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
J Cell Biol 181:65-78. 2008..We conclude that tight regulation of the Bora protein by its synthesis and degradation is critical for cell cycle progression... - RCS1, a substrate of APC/C, controls the metaphase to anaphase transitionWei Meng Zhao
Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
Proc Natl Acad Sci U S A 105:13415-20. 2008..Our study uncovers a complex regulatory network for the metaphase-to-anaphase transition... - The N-terminal domain of DDA3 regulates the spindle-association of the microtubule depolymerase Kif2a and controls the mitotic function of DDA3Chang Young Jang
Department of Biological Sciences, Stanford University, Stanford, CA, USA
Cell Cycle 8:3165-71. 2009..The C-terminal domain confers its ability to associate with the mitotic spindle, while the regulatory N-terminal domain controls the microtubule-binding by the C-terminal domain and determines the cellular activity of the DDA3 protein... 
Mitotic kinases regulate MT-polymerizing/MT-bundling activity of DDA3Chang Young Jang
Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
Biochem Biophys Res Commun 408:174-9. 2011..We conclude that kinases control the function of DDA3 in the cell cycle by regulating its MT-polymerizing/bundling activities through sequential phosphorylation...- MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesisWei Meng Zhao
Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
Proc Natl Acad Sci U S A 102:13158-63. 2005..We conclude that MgcRacGAP controls the initiation of cytokinesis by regulating ECT2, which in turn induces the assembly of the contractile ring and triggers the ingression of the cleavage furrow... - Anaphase-promoting complex/cyclosome controls the stability of TPX2 during mitotic exitScott Stewart
Department of Biological Sciences, Stanford University, 337 Campus Drive, Room 137, Stanford, CA 94305 5020, USA
Mol Cell Biol 25:10516-27. 2005..We conclude that APC/C(Cdh1) controls the stability of TPX2, thereby ensuring accurate regulation of the spindle assembly in the cell cycle... - Loading of the 3F3/2 antigen onto kinetochores is dependent on the ordered assembly of the spindle checkpoint proteinsOi Kwan Wong
Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
Mol Biol Cell 17:4390-9. 2006..The characterization of this ordered assembly pathway provides a framework for the biochemical mechanism of the checkpoint signaling and will aid in the eventual identification of the 3F3/2 substrate... - CKAP2 is a spindle-associated protein degraded by APC/C-Cdh1 during mitotic exitAkiko Seki
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
J Biol Chem 282:15103-13. 2007..We concluded that CKAP2 is a physiological substrate of APC/C during mitotic exit and that a tight regulation of the CKAP2 protein level is critical for the normal mitotic progression... - Phospho-regulation of DDA3 function in mitosisChang Young Jang
Department of Biological Sciences, Stanford University, CA 94305 5020, USA
Biochem Biophys Res Commun 393:259-63. 2010..We conclude that the mitotic function of DDA3 is regulated by phosphorylation on the Ser225 residue... - Plx1 is the 3F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochoresOi Kwan Wong
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
J Cell Biol 170:709-19. 2005..Thus, Plx1 couples the tension signal to cellular responses through phosphorylating the 3F3/2 epitope and targeting structural and checkpoint proteins to kinetochores... - Destruction box-dependent degradation of aurora B is mediated by the anaphase-promoting complex/cyclosome and Cdh1Scott Stewart
Department of Biological Sciences, Stanford University, Stanford, California 94305-5020, USA
Cancer Res 65:8730-5. 2005..We conclude that, as a key mitotic regulator, Aurora B is regulated both by its activation during early mitosis and by its destruction by APC/C-Cdh1 in late mitosis and in G1... - HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore captureJim Wong
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
J Cell Biol 173:879-91. 2006..Thus, HURP controls spindle stability and dynamics to achieve efficient kinetochore capture at prometaphase, timely chromosome congression to the metaphase plate, and proper interkinetochore tension for anaphase initiation... - Mechanism, function and regulation of microtubule-dependent microtubule amplification in mitosisHui Zhu
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
Mol Cells 27:1-3. 2009..We will review here our current understanding on the molecular mechanism, the physiological function and the cell-cycle regulation of MT amplification... - Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochoresJennifer G DeLuca
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Curr Biol 13:2103-9. 2003..Our observations support a model in which Nuf2 and Hec1 function to prevent microtubule-dependent stripping of Mad1 and Mad2 from kinetochores that have not yet formed stable kinetochore-microtubule attachments... - Breast cancer-specific gene 1 interacts with the mitotic checkpoint kinase BubR1Anu Gupta
Department of Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA
Oncogene 22:7593-9. 2003..Taken together, our novel findings suggest that BCSG1 may accelerate the progression of breast cancer at least in part by compromising the mitotic checkpoint control through inactivation of BubR1... - Chfr is required for tumor suppression and Aurora A regulationXiaochun Yu
Department of Oncology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
Nat Genet 37:401-6. 2005..Collectively, our data suggest that Chfr is a tumor suppressor and ensures chromosomal stability by controlling the expression levels of key mitotic proteins such as Aurora A... - Anaphase onset does not require the microtubule-dependent depletion of kinetochore and centromere-binding proteinsJulie C Canman
University of North Carolina, Department of Biology, 607 Fordham Hall, CB3280, Chapel Hill, NC 27599, USA
J Cell Sci 115:3787-95. 2002.... - Inducing precocious anaphase in cultured mammalian cellsJulie C Canman
University of North Carolina, Department of Biology, 607 Fordham Hall, CB 3280, Chapel Hill, NC 27599 3280, USA
Cell Motil Cytoskeleton 52:61-5. 2002..This paper compares new and old methods of overriding the spindle checkpoint in prometaphase mammalian tissue culture cells... - Cyclin B and E2F-1 expression in prostate carcinoma cells treated with the novel retinoid CD437 are regulated by the ubiquitin-mediated pathwayLulu Farhana
John D Dingell VA Medical Center and Karmanos Cancer Institute, and Department Internal Medicine, Wayne State University, Detroit, Michigan 48201, USA
Cancer Res 62:3842-9. 2002..Thus, CD437 modulates the expression of E2F-1 and cyclin B through the simultaneous stimulation and inhibition of the cyclin B and E2F-1 E3 ligases, respectively... - Spindle checkpoint protein dynamics at kinetochores in living cellsBonnie J Howell
Department of Biology, CB#3280, 607 Fordham Hall, University of North Carolina, Chapel Hill, NC 27599 USA
Curr Biol 14:953-64. 2004.... - Determining the position of the cell division planeJulie C Canman
University of North Carolina, Department of Biology, 607 Fordham Hall, CB #3280, Chapel Hill, North Carolina 27699-3280, USA
Nature 424:1074-8. 2003..Our data are consistent with a model in which chromosomes supply microtubules with factors that promote microtubule stability and furrowing...