Guowei Fang

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. pmc The checkpoint protein Chfr is a ligase that ubiquitinates Plk1 and inhibits Cdc2 at the G2 to M transition
    Dongmin Kang
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    J Cell Biol 156:249-59. 2002
  2. pmc Checkpoint protein BubR1 acts synergistically with Mad2 to inhibit anaphase-promoting complex
    Guowei Fang
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    Mol Biol Cell 13:755-66. 2002
  3. ncbi request reprint Anillin is a substrate of anaphase-promoting complex/cyclosome (APC/C) that controls spatial contractility of myosin during late cytokinesis
    Wei Meng Zhao
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    J Biol Chem 280:33516-24. 2005
  4. ncbi request reprint Centromere cohesion: regulating the guardian
    Lin Fang
    Department of Biological Sciences, Lokey Chemical Biology Building, Stanford University, Stanford, CA 94305 5020, USA
    Cell Res 17:664-5. 2007
  5. pmc Bora and the kinase Aurora a cooperatively activate the kinase Plk1 and control mitotic entry
    Akiko Seki
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Science 320:1655-8. 2008
  6. pmc Plk1 and Aurora A regulate the depolymerase activity and the cellular localization of Kif2a
    Chang Young Jang
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Sci 122:1334-41. 2009
  7. ncbi request reprint A Xenopus cell-free system for analysis of the Chfr ubiquitin ligase involved in control of mitotic entry
    Dongmin Kang
    Department of Biological Sciences, Stanford University, CA, USA
    Methods Mol Biol 280:229-43. 2004
  8. pmc DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement
    Chang Young Jang
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 181:255-67. 2008
  9. pmc Cdk1 phosphorylation of BubR1 controls spindle checkpoint arrest and Plk1-mediated formation of the 3F3/2 epitope
    Oi Kwan Wong
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 179:611-7. 2007
  10. pmc FAM29A, a target of Plk1 regulation, controls the partitioning of NEDD1 between the mitotic spindle and the centrosomes
    Hui Zhu
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Sci 122:2750-9. 2009

Collaborators

Detail Information

Publications35

  1. pmc The checkpoint protein Chfr is a ligase that ubiquitinates Plk1 and inhibits Cdc2 at the G2 to M transition
    Dongmin Kang
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    J Cell Biol 156:249-59. 2002
    ..Thus, the Chfr pathway represents a novel checkpoint pathway that regulates the entry into mitosis by ubiquitin-dependent proteolysis...
  2. pmc Checkpoint protein BubR1 acts synergistically with Mad2 to inhibit anaphase-promoting complex
    Guowei Fang
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    Mol Biol Cell 13:755-66. 2002
    ..Thus, BubR1 and Mad2 act cooperatively to prevent premature separation of sister chromatids by directly inhibiting APC...
  3. ncbi request reprint Anillin is a substrate of anaphase-promoting complex/cyclosome (APC/C) that controls spatial contractility of myosin during late cytokinesis
    Wei Meng Zhao
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    J Biol Chem 280:33516-24. 2005
    ..We concluded that anillin functions to maintain the localization of active myosin, thereby ensuring the spatial control of concerted contraction during cytokinesis...
  4. ncbi request reprint Centromere cohesion: regulating the guardian
    Lin Fang
    Department of Biological Sciences, Lokey Chemical Biology Building, Stanford University, Stanford, CA 94305 5020, USA
    Cell Res 17:664-5. 2007
  5. pmc Bora and the kinase Aurora a cooperatively activate the kinase Plk1 and control mitotic entry
    Akiko Seki
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Science 320:1655-8. 2008
    ..Thus, Bora and Aur-A control mitotic entry, which provides a mechanism for one of the most important yet ill-defined events in the cell cycle...
  6. pmc Plk1 and Aurora A regulate the depolymerase activity and the cellular localization of Kif2a
    Chang Young Jang
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Sci 122:1334-41. 2009
    ....
  7. ncbi request reprint A Xenopus cell-free system for analysis of the Chfr ubiquitin ligase involved in control of mitotic entry
    Dongmin Kang
    Department of Biological Sciences, Stanford University, CA, USA
    Methods Mol Biol 280:229-43. 2004
    ....
  8. pmc DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement
    Chang Young Jang
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 181:255-67. 2008
    ..Thus, DDA3 represents a new class of MT-destabilizing protein that controls spindle dynamics and mitotic progression by regulating MT depolymerases...
  9. pmc Cdk1 phosphorylation of BubR1 controls spindle checkpoint arrest and Plk1-mediated formation of the 3F3/2 epitope
    Oi Kwan Wong
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 179:611-7. 2007
    ..Thus, Cdk1-mediated phosphorylation of BubR1 controls checkpoint arrest and promotes the formation of the kinetochore 3F3/2 epitope...
  10. pmc FAM29A, a target of Plk1 regulation, controls the partitioning of NEDD1 between the mitotic spindle and the centrosomes
    Hui Zhu
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Sci 122:2750-9. 2009
    ..FAM29A controls the relative contributions of these two pathways to microtubule polymerization during mitosis...
  11. pmc Cep55, a microtubule-bundling protein, associates with centralspindlin to control the midbody integrity and cell abscission during cytokinesis
    Wei Meng Zhao
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Mol Biol Cell 17:3881-96. 2006
    ..Our study defines a cellular mechanism that links centralspindlin to Cep55, which, in turn, controls the midbody structure and membrane fusion at the terminal stage of cytokinesis...
  12. pmc FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle
    Hui Zhu
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 183:835-48. 2008
    ..Our study provides a biochemical mechanism for MT-dependent MT amplification and for the maturation of kinetochore fibers in mammalian cells...
  13. pmc Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule-binding domain
    Jim Wong
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Mol Biol Cell 19:2083-91. 2008
    ..We concluded that phosphorylation of HURP by Aurora A provides a regulatory mechanism for the control of spindle assembly and function...
  14. ncbi request reprint SKAP associates with kinetochores and promotes the metaphase-to-anaphase transition
    Lin Fang
    Department of Biological Sciences, Stanford University, Stanford, CA, USA
    Cell Cycle 8:2819-27. 2009
    ....
  15. pmc Plk1- and beta-TrCP-dependent degradation of Bora controls mitotic progression
    Akiko Seki
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 181:65-78. 2008
    ..We conclude that tight regulation of the Bora protein by its synthesis and degradation is critical for cell cycle progression...
  16. pmc RCS1, a substrate of APC/C, controls the metaphase to anaphase transition
    Wei Meng Zhao
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Proc Natl Acad Sci U S A 105:13415-20. 2008
    ..Our study uncovers a complex regulatory network for the metaphase-to-anaphase transition...
  17. ncbi request reprint The N-terminal domain of DDA3 regulates the spindle-association of the microtubule depolymerase Kif2a and controls the mitotic function of DDA3
    Chang Young Jang
    Department of Biological Sciences, Stanford University, Stanford, CA, USA
    Cell Cycle 8:3165-71. 2009
    ..The C-terminal domain confers its ability to associate with the mitotic spindle, while the regulatory N-terminal domain controls the microtubule-binding by the C-terminal domain and determines the cellular activity of the DDA3 protein...
  18. doi request reprint Mitotic kinases regulate MT-polymerizing/MT-bundling activity of DDA3
    Chang Young Jang
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Biochem Biophys Res Commun 408:174-9. 2011
    ..We conclude that kinases control the function of DDA3 in the cell cycle by regulating its MT-polymerizing/bundling activities through sequential phosphorylation...
  19. pmc MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis
    Wei Meng Zhao
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Proc Natl Acad Sci U S A 102:13158-63. 2005
    ..We conclude that MgcRacGAP controls the initiation of cytokinesis by regulating ECT2, which in turn induces the assembly of the contractile ring and triggers the ingression of the cleavage furrow...
  20. pmc Anaphase-promoting complex/cyclosome controls the stability of TPX2 during mitotic exit
    Scott Stewart
    Department of Biological Sciences, Stanford University, 337 Campus Drive, Room 137, Stanford, CA 94305 5020, USA
    Mol Cell Biol 25:10516-27. 2005
    ..We conclude that APC/C(Cdh1) controls the stability of TPX2, thereby ensuring accurate regulation of the spindle assembly in the cell cycle...
  21. pmc Loading of the 3F3/2 antigen onto kinetochores is dependent on the ordered assembly of the spindle checkpoint proteins
    Oi Kwan Wong
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Mol Biol Cell 17:4390-9. 2006
    ..The characterization of this ordered assembly pathway provides a framework for the biochemical mechanism of the checkpoint signaling and will aid in the eventual identification of the 3F3/2 substrate...
  22. ncbi request reprint CKAP2 is a spindle-associated protein degraded by APC/C-Cdh1 during mitotic exit
    Akiko Seki
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    J Biol Chem 282:15103-13. 2007
    ..We concluded that CKAP2 is a physiological substrate of APC/C during mitotic exit and that a tight regulation of the CKAP2 protein level is critical for the normal mitotic progression...
  23. pmc Phospho-regulation of DDA3 function in mitosis
    Chang Young Jang
    Department of Biological Sciences, Stanford University, CA 94305 5020, USA
    Biochem Biophys Res Commun 393:259-63. 2010
    ..We conclude that the mitotic function of DDA3 is regulated by phosphorylation on the Ser225 residue...
  24. pmc Plx1 is the 3F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores
    Oi Kwan Wong
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 170:709-19. 2005
    ..Thus, Plx1 couples the tension signal to cellular responses through phosphorylating the 3F3/2 epitope and targeting structural and checkpoint proteins to kinetochores...
  25. ncbi request reprint Destruction box-dependent degradation of aurora B is mediated by the anaphase-promoting complex/cyclosome and Cdh1
    Scott Stewart
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    Cancer Res 65:8730-5. 2005
    ..We conclude that, as a key mitotic regulator, Aurora B is regulated both by its activation during early mitosis and by its destruction by APC/C-Cdh1 in late mitosis and in G1...
  26. pmc HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture
    Jim Wong
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 173:879-91. 2006
    ..Thus, HURP controls spindle stability and dynamics to achieve efficient kinetochore capture at prometaphase, timely chromosome congression to the metaphase plate, and proper interkinetochore tension for anaphase initiation...
  27. doi request reprint Mechanism, function and regulation of microtubule-dependent microtubule amplification in mitosis
    Hui Zhu
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Mol Cells 27:1-3. 2009
    ..We will review here our current understanding on the molecular mechanism, the physiological function and the cell-cycle regulation of MT amplification...
  28. ncbi request reprint Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores
    Jennifer G DeLuca
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Curr Biol 13:2103-9. 2003
    ..Our observations support a model in which Nuf2 and Hec1 function to prevent microtubule-dependent stripping of Mad1 and Mad2 from kinetochores that have not yet formed stable kinetochore-microtubule attachments...
  29. ncbi request reprint Breast cancer-specific gene 1 interacts with the mitotic checkpoint kinase BubR1
    Anu Gupta
    Department of Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA
    Oncogene 22:7593-9. 2003
    ..Taken together, our novel findings suggest that BCSG1 may accelerate the progression of breast cancer at least in part by compromising the mitotic checkpoint control through inactivation of BubR1...
  30. ncbi request reprint Chfr is required for tumor suppression and Aurora A regulation
    Xiaochun Yu
    Department of Oncology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
    Nat Genet 37:401-6. 2005
    ..Collectively, our data suggest that Chfr is a tumor suppressor and ensures chromosomal stability by controlling the expression levels of key mitotic proteins such as Aurora A...
  31. ncbi request reprint Anaphase onset does not require the microtubule-dependent depletion of kinetochore and centromere-binding proteins
    Julie C Canman
    University of North Carolina, Department of Biology, 607 Fordham Hall, CB3280, Chapel Hill, NC 27599, USA
    J Cell Sci 115:3787-95. 2002
    ....
  32. ncbi request reprint Inducing precocious anaphase in cultured mammalian cells
    Julie C Canman
    University of North Carolina, Department of Biology, 607 Fordham Hall, CB 3280, Chapel Hill, NC 27599 3280, USA
    Cell Motil Cytoskeleton 52:61-5. 2002
    ..This paper compares new and old methods of overriding the spindle checkpoint in prometaphase mammalian tissue culture cells...
  33. ncbi request reprint Cyclin B and E2F-1 expression in prostate carcinoma cells treated with the novel retinoid CD437 are regulated by the ubiquitin-mediated pathway
    Lulu Farhana
    John D Dingell VA Medical Center and Karmanos Cancer Institute, and Department Internal Medicine, Wayne State University, Detroit, Michigan 48201, USA
    Cancer Res 62:3842-9. 2002
    ..Thus, CD437 modulates the expression of E2F-1 and cyclin B through the simultaneous stimulation and inhibition of the cyclin B and E2F-1 E3 ligases, respectively...
  34. ncbi request reprint Spindle checkpoint protein dynamics at kinetochores in living cells
    Bonnie J Howell
    Department of Biology, CB 3280, 607 Fordham Hall, University of North Carolina, Chapel Hill, NC 27599 USA
    Curr Biol 14:953-64. 2004
    ....
  35. ncbi request reprint Determining the position of the cell division plane
    Julie C Canman
    University of North Carolina, Department of Biology, 607 Fordham Hall, CB 3280, Chapel Hill, North Carolina 27699 3280, USA
    Nature 424:1074-8. 2003
    ..Our data are consistent with a model in which chromosomes supply microtubules with factors that promote microtubule stability and furrowing...