Research Topics
Genomes and Genes | M S CyertSummaryAffiliation: Stanford University Country: USA Publications
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Publications
Regulation of cation balance in Saccharomyces cerevisiaeMartha S Cyert
Department of Biology, Stanford University, Stanford, CA 94305 5020, USA
Genetics 193:677-713. 2013..e., either limited availability or toxic levels in the environment...
Calcineurin signaling in Saccharomyces cerevisiae: how yeast go crazy in response to stressMartha S Cyert
Department of Biological Sciences, Stanford University, Stanford, CA 94306, USA
Biochem Biophys Res Commun 311:1143-50. 2003..Recent studies concerning the regulation of Crz1p by calcineurin are discussed in this review and the mechanisms by which calcineurin controls gene expression in yeast and mammalian cells are compared...- Genetic analysis of calmodulin and its targets in Saccharomyces cerevisiaeM S Cyert
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
Annu Rev Genet 35:647-72. 2001..Thus, calmodulin performs important physiological functions in yeast cells in both its Ca2+-bound and Ca2+-free form... - Calcineurin acts through the CRZ1/TCN1-encoded transcription factor to regulate gene expression in yeastA M Stathopoulos
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
Genes Dev 11:3432-44. 1997..Thus, in response to multiple signals, calcineurin acts through the Crz1p transcription factor to differentially regulate the expression of several target genes in yeast... - The product of HUM1, a novel yeast gene, is required for vacuolar Ca2+/H+ exchange and is related to mammalian Na+/Ca2+ exchangersT C Pozos
Department of Biological Sciences, Stanford University, California 94305 5020, USA
Mol Cell Biol 16:3730-41. 1996..Vacuolar membrane vesicles from hum1 mutants lack all Ca2+/H+ antiport activity, demonstrating that Hum1p catalyzes the exchange of Ca2+ for H+ across the yeast vacuolar membrane... - Calcineurin-dependent nuclear import of the transcription factor Crz1p requires Nmd5pR S Polizotto
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
J Cell Biol 154:951-60. 2001..Finally, we demonstrate that residues in both the NH2- and COOH-terminal portions of Crz1p are required for regulated Crz1p binding to Nmd5p, supporting a model of NLS masking for regulating Crz1p nuclear import... - The eukaryotic response regulator Skn7p regulates calcineurin signaling through stabilization of Crz1pK E Williams
Department of Biological Science, Stanford University, Stanford, CA 94305 5020, USA
EMBO J 20:3473-83. 2001..The DNA-binding domain of Skn7p is also required for binding to Crz1p and calcineurin in vitro. Thus, we propose that Skn7p protects Crz1p from degradation by binding to it and calcineurin through its DNA-binding domain... - Yeast calcineurin regulates nuclear localization of the Crz1p transcription factor through dephosphorylationA Stathopoulos-Gerontides
Deptartment of Biological Sciences, Stanford University, Stanford, California 94305 5020 USA
Genes Dev 13:798-803. 1999..Thus, the mechanism of Ca2+/calcineurin-dependent signaling shows remarkable conservation between yeast and mammalian cells... - Ion tolerance of Saccharomyces cerevisiae lacking the Ca2+/CaM-dependent phosphatase (calcineurin) is improved by mutations in URE2 or PMA1J L Withee
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
Genetics 149:865-78. 1998..These studies further characterize the roles that URE2 and PMA1 play in regulating intracellular ion homeostasis... - Identification of a novel region critical for calcineurin function in vivo and in vitroB Jiang
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
J Biol Chem 274:18543-51. 1999.... - Hph1p and Hph2p, novel components of calcineurin-mediated stress responses in Saccharomyces cerevisiaeVictoria L Heath
Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
Eukaryot Cell 3:695-704. 2004..Thus, Hph1p and Hph2p are components of a novel Ca2+- and calcineurin-regulated response required to promote growth under conditions of high Na+, alkaline pH, and cell wall stress... - Luv1p/Rki1p/Tcs3p/Vps54p, a yeast protein that localizes to the late Golgi and early endosome, is required for normal vacuolar morphologyM J Conboy
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
Mol Biol Cell 11:2429-43. 2000..Computer analysis predicts a short coiled-coil domain in Luv1p. We propose that this protein maintains traffic through or the integrity of the early endosome and that this function is required for proper vacuolar morphology... - Integration of stress responses: modulation of calcineurin signaling in Saccharomyces cerevisiae by protein kinase AKimberly A Kafadar
Department of Biological Sciences, Stanford University, 337 Campus Dr, Lokey Building, Stanford, CA 94305 5020, USA
Eukaryot Cell 3:1147-53. 2004..Thus, these studies identify a specific biochemical mechanism through which the activities of multiple stress-activated signaling pathways are integrated in vivo... - Negative regulation of calcineurin signaling by Hrr25p, a yeast homolog of casein kinase IKimberly A Kafadar
Department of Biological Sciences Stanford University, Stanford, California, 94305 5020, USA
Genes Dev 17:2698-708. 2003..These findings represent the first identification of a negative regulator for Crz1p, and establish a novel physiological role for Hrr25p in antagonizing calcineurin signaling... - A conserved docking site modulates substrate affinity for calcineurin, signaling output, and in vivo functionJagoree Roy
Department of Biological Sciences, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA
Mol Cell 25:889-901. 2007..Thus, calcineurin-substrate affinity determines the Ca(2+) concentration dependence and output of signaling in vivo as well as the balance between different branches of calcineurin signaling in an overall biological response... - Calcineurin-dependent regulation of Crz1p nuclear export requires Msn5p and a conserved calcineurin docking siteLeila M Boustany
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
Genes Dev 16:608-19. 2002..Therefore, in yeast as in mammals, a docking site is required to target calcineurin to its substrate such that it can dephosphorylate it efficiently... - Genome-wide analysis of gene expression regulated by the calcineurin/Crz1p signaling pathway in Saccharomyces cerevisiaeHiroyuki Yoshimoto
Department of Biological Sciences, Stanford University, 371 Serra Mall, Stanford, CA 94305-5020, USA
J Biol Chem 277:31079-88. 2002..A similar sequence, 5'-GAGGCTG-3', was identified as a common sequence motif in the upstream regions of calcineurin/ Crz1p-dependent genes. This finding is consistent with direct regulation of these genes by Crz1p... - Calcineurin, the Ca2+/calmodulin-dependent protein phosphatase, is essential in yeast mutants with cell integrity defects and in mutants that lack a functional vacuolar H(+)-ATPaseP Garrett-Engele
Department of Biological Sciences, Stanford University, California 94305 5020
Mol Cell Biol 15:4103-14. 1995..We discuss possible roles for calcineurin in regulating intracellular ion homeostasis and in maintaining cell integrity... - Slm1 and slm2 are novel substrates of the calcineurin phosphatase required for heat stress-induced endocytosis of the yeast uracil permeaseGeert Bultynck
Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
Mol Cell Biol 26:4729-45. 2006.... - Molecular analysis reveals localization of Saccharomyces cerevisiae protein kinase C to sites of polarized growth and Pkc1p targeting to the nucleus and mitotic spindleValerie Denis
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
Eukaryot Cell 4:36-45. 2005..This novel nuclear and spindle localization of Pkc1p may provide a molecular explanation for previous observations that suggest a role for Pkc1p in regulating microtubule function... - Internal Ca(2+) release in yeast is triggered by hypertonic shock and mediated by a TRP channel homologueValerie Denis
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
J Cell Biol 156:29-34. 2002..These studies reveal a novel mechanism of internal Ca(2+) release and establish a new function for TRP channels... - Curcumin inhibits growth of Saccharomyces cerevisiae through iron chelationSteven Minear
Department of Biology, Stanford University, Stanford, CA 94305 5020, USA
Eukaryot Cell 10:1574-81. 2011..These findings suggest that curcumin-induced iron starvation is conserved from yeast to humans and underlies curcumin's medicinal properties... - Hph1 and Hph2 are novel components of the Sec63/Sec62 posttranslational translocation complex that aid in vacuolar proton ATPase biogenesisFrancisco J Piña
Department of Biology, 371 Serra Drive, Stanford University, Stanford, CA 94305 5020, USA
Eukaryot Cell 10:63-71. 2011..We propose that Hph1 and Hph2 aid Sec63/Sec62-mediated translocation of specific proteins, including factors that promote efficient biogenesis of V-ATPase, to support yeast cell survival during environmental stress... - Renaming the DSCR1/Adapt78 gene family as RCAN: regulators of calcineurinKelvin J A Davies
Ethel Percy Andrus Gerontology Center, and Division of Molecular and Computational Biology, The University of Southern California, Los Angeles, CA 90089-0191, USA
FASEB J 21:3023-8. 2007
Research Grants
- Role of Ca2+/Calcineurin Signaling in S.CerevisiaeMartha Cyert; Fiscal Year: 2009..3) Identify the role of calcineurin interacting proteins in calcineurin signaling pathways. 4) Identify additional calcineurin substrates using novel proteomic and genomic screening methods. ..
- Role of Ca2+/Calcineurin Signaling in S.CerevisiaeMartha Cyert; Fiscal Year: 2009..3) Identify the role of calcineurin interacting proteins in calcineurin signaling pathways. 4) Identify additional calcineurin substrates using novel proteomic and genomic screening methods. ..
- Role of Ca2+/Calcineurin Signaling in S. cerevisiaeMartha Cyert; Fiscal Year: 2005..cerevisiae, because of the many experimental advantages offered by this organism. However, we believe our findings will establish general principles that will apply to calcineurin-dependent signaling in all cells. ..
- YEAST PP2B PHOSPHATASES--ROLE IN PHEROMONE ADAPTATIONMartha Cyert; Fiscal Year: 2001..4) Identify substrates of calcineurin by identifying gene products that physically interact with calcineurin in vivo and by testing specific candidate proteins in vitro for calcineurin-dependent dephosphorylation. ..
- Role of Ca2+/Calcineurin Signaling in S. cerevisiaeMartha S Cyert; Fiscal Year: 2010..This research studies, at the biochemical level, the functions of calcineurin in a simple, experimentally tractable organism, i.e. yeast, and will serve as a model for understanding the actions of calcineurin in human cells. ..