MICHELE CALOS

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. pmc Phage integrases for the construction and manipulation of transgenic mammals
    Roger P Hollis
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    Reprod Biol Endocrinol 1:79. 2003
  2. ncbi request reprint Therapeutic applications of the ΦC31 integrase system
    Christopher L Chavez
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    Curr Gene Ther 11:375-81. 2011
  3. pmc Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31
    Amy C Groth
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
    Genetics 166:1775-82. 2004
  4. ncbi request reprint The phiC31 integrase system for gene therapy
    Michele P Calos
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    Curr Gene Ther 6:633-45. 2006
  5. ncbi request reprint Site-specific integration with phiC31 integrase for prolonged expression of therapeutic genes
    Daniel S Ginsburg
    Department of Genetics Stanford University School of Medicine Stanford, California 94305, USA
    Adv Genet 54:179-87. 2005
  6. ncbi request reprint Long-term increase in mVEGF164 in mouse hindlimb muscle mediated by phage phiC31 integrase after nonviral DNA delivery
    Joylette L Portlock
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Hum Gene Ther 17:871-6. 2006
  7. ncbi request reprint PhiC31 integrase mediates integration in cultured synovial cells and enhances gene expression in rabbit joints
    Annahita Keravala
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    J Gene Med 8:1008-17. 2006
  8. ncbi request reprint A diversity of serine phage integrases mediate site-specific recombination in mammalian cells
    Annahita Keravala
    Department of Genetics, M 334, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305 5120, USA
    Mol Genet Genomics 276:135-46. 2006
  9. ncbi request reprint Creating transgenic Drosophila by microinjecting the site-specific phiC31 integrase mRNA and a transgene-containing donor plasmid
    Matthew P Fish
    Department of Developmental Biology and Howard Hughes Medical Institute, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305 5120, USA
    Nat Protoc 2:2325-31. 2007
  10. pmc Long-term transgene expression in mouse neural progenitor cells modified with phiC31 integrase
    Annahita Keravala
    Department of Genetics, Stanford University School of Medicine, M 334, 300 Pasteur Drive, Stanford, CA 94305 5120, United States
    J Neurosci Methods 173:299-305. 2008

Research Grants

  1. Retinal Gene Therapy by Site-Specific Integration
    MICHELE CALOS; Fiscal Year: 2006
  2. Transferring integrase technology to animals
    MICHELE CALOS; Fiscal Year: 2006
  3. Custom integration tools for functional genomics
    MICHELE CALOS; Fiscal Year: 2003
  4. Transferring integrase technology to animals
    MICHELE CALOS; Fiscal Year: 2003
  5. SITE-SPECIFIC INTEGRATION FOR GENE THERAPY
    MICHELE CALOS; Fiscal Year: 2003

Collaborators

Detail Information

Publications32

  1. pmc Phage integrases for the construction and manipulation of transgenic mammals
    Roger P Hollis
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    Reprod Biol Endocrinol 1:79. 2003
    ..The results reviewed here introduce these bacteriophage integrases as tools for site-specific modification of the genome for the creation and manipulation of transgenic mammals...
  2. ncbi request reprint Therapeutic applications of the ΦC31 integrase system
    Christopher L Chavez
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    Curr Gene Ther 11:375-81. 2011
    ..We conclude that gene therapy strategies utilizing ΦC31 integrase offer great promise for the development of treatments in the future...
  3. pmc Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31
    Amy C Groth
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
    Genetics 166:1775-82. 2004
    ..These experiments demonstrate the potential for precise genetic engineering of the Drosophila genome with the phiC31 integrase system and will likely benefit research in Drosophila and other insects...
  4. ncbi request reprint The phiC31 integrase system for gene therapy
    Michele P Calos
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    Curr Gene Ther 6:633-45. 2006
    ..Ongoing and planned improvements to the phage integrase system are discussed. We conclude that gene therapy strategies using phiC31 integrase and its derivatives offer great promise for success in the near term...
  5. ncbi request reprint Site-specific integration with phiC31 integrase for prolonged expression of therapeutic genes
    Daniel S Ginsburg
    Department of Genetics Stanford University School of Medicine Stanford, California 94305, USA
    Adv Genet 54:179-87. 2005
    ..Development of phiC31 integrase-based vectors is still underway, but it has already been shown to provide long-term expression through site-specific integration...
  6. ncbi request reprint Long-term increase in mVEGF164 in mouse hindlimb muscle mediated by phage phiC31 integrase after nonviral DNA delivery
    Joylette L Portlock
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Hum Gene Ther 17:871-6. 2006
    ..These results suggest the possible utility of the phiC31 integrase system to treat ischemic disease...
  7. ncbi request reprint PhiC31 integrase mediates integration in cultured synovial cells and enhances gene expression in rabbit joints
    Annahita Keravala
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    J Gene Med 8:1008-17. 2006
    ..We report a novel approach for non-viral gene therapy to joints that utilizes phage phiC31 integrase to bring about unidirectional genomic integration...
  8. ncbi request reprint A diversity of serine phage integrases mediate site-specific recombination in mammalian cells
    Annahita Keravala
    Department of Genetics, M 334, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305 5120, USA
    Mol Genet Genomics 276:135-46. 2006
    ..The other integrases did not mediate integration at genomic sequences at a frequency above background. These site-specific integrases represent valuable new tools for manipulating eukaryotic genomes...
  9. ncbi request reprint Creating transgenic Drosophila by microinjecting the site-specific phiC31 integrase mRNA and a transgene-containing donor plasmid
    Matthew P Fish
    Department of Developmental Biology and Howard Hughes Medical Institute, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305 5120, USA
    Nat Protoc 2:2325-31. 2007
    ..The whole procedure, from injection to established transgenic stocks, can be completed in three generations (approximately 1 month) and can be adapted for other types of transgenesis and mRNA injections in Drosophila...
  10. pmc Long-term transgene expression in mouse neural progenitor cells modified with phiC31 integrase
    Annahita Keravala
    Department of Genetics, Stanford University School of Medicine, M 334, 300 Pasteur Drive, Stanford, CA 94305 5120, United States
    J Neurosci Methods 173:299-305. 2008
    ..Our results demonstrate that the phiC31 integrase system produces stable transgene expression in adult mNPCs and their progeny and may be useful in strategies for combating neurodegenerative disorders...
  11. pmc Enhancement of plasmid-mediated gene therapy for muscular dystrophy by directed plasmid integration
    Carmen Bertoni
    Department of Neurology, Stanford University School of Medicine, Stanford, CA 94305 5235, USA
    Proc Natl Acad Sci U S A 103:419-24. 2006
    ..These data demonstrate the importance of both the level and distribution of dystrophin expression to achieve therapeutic efficacy, and that the efficacy can be enhanced by targeted plasmid integration...
  12. ncbi request reprint phiC31 integrase confers genomic integration and long-term transgene expression in rat retina
    Thomas W Chalberg
    Department of Genetics, Stanford University School of Medicine, CA 94305, USA
    Invest Ophthalmol Vis Sci 46:2140-6. 2005
    ....
  13. pmc Phage TP901-1 site-specific integrase functions in human cells
    Stephanie M Stoll
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305 5120, USA
    J Bacteriol 184:3657-63. 2002
    ..The TP901-1 phage integrase thus represents a new reagent for manipulating DNA in living mammalian cells...
  14. ncbi request reprint Stable nonviral genetic correction of inherited human skin disease
    Susana Ortiz-Urda
    VA Palo Alto Healthcare System and the Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA
    Nat Med 8:1166-70. 2002
    ..These findings establish a practical approach to nonviral genetic correction of severe human genetic disorders requiring stable genomic integration of large DNA sequences...
  15. ncbi request reprint PhiC31 integrase-mediated nonviral genetic correction of junctional epidermolysis bullosa
    Susana Ortiz-Urda
    VA Palo Alto Healthcare System and Program in Epithelial Biology, Stanford University School of Medicine, 269 Campus Drive, Stanford, CA 94305, USA
    Hum Gene Ther 14:923-8. 2003
    ..Furthermore, corrected JEB tissue restored hemidesmosome formation and abolished histologic evidence of subepidermal blistering. These findings provide an approach to durable nonviral correction of JEB...
  16. ncbi request reprint Phage integrases: biology and applications
    Amy C Groth
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    J Mol Biol 335:667-78. 2004
    ..Directed evolution can be used to increase further the affinity of an integrase for a particular native sequence, opening up additional applications for genomic modification...
  17. doi request reprint Site-specific chromosomal integration mediated by phiC31 integrase
    Annahita Keravala
    Department of Genetics, Stanford University School of Medicine, Stanford, CA
    Methods Mol Biol 435:165-73. 2008
    ..This integration system based on phiC31 integrase supplies a simple method to obtain repeated integration at the same chromosomal site in mammalian cells...
  18. ncbi request reprint Extrachromosomal plasmid vectors for gene therapy
    Stephanie M Stoll
    Department of Genetics, Stanford University School of Medicine, CA 94305 5120, USA
    Curr Opin Mol Ther 4:299-305. 2002
    ..This review will discuss the advantages of extrachromosomal DNA as a gene therapy vector, highlighting recent advances and successes in its use in vivo...
  19. ncbi request reprint Integration specificity of phage phiC31 integrase in the human genome
    Thomas W Chalberg
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 95305 5120, USA
    J Mol Biol 357:28-48. 2006
    ..An analysis of the safety of integration sites in terms of proximity to cancer genes suggested minimal cancer risk. We conclude that integration systems derived from phiC31 integrase have great potential utility...
  20. ncbi request reprint Factoring nonviral gene therapy into a cure for hemophilia A
    Vanessa Gabrovsky
    Stanford University School of Medicine, Department of Genetics, Stanford, CA 94305 5120, USA
    Curr Opin Mol Ther 10:464-70. 2008
    ..New types of nonviral strategies, such as DNA integrating vectors, and the success of several nonviral animal studies, suggest that nonviral gene therapy has curative potential and justifies its clinical development...
  21. ncbi request reprint Site-specific genomic integration produces therapeutic Factor IX levels in mice
    Eric C Olivares
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Nat Biotechnol 20:1124-8. 2002
    ..Integration was documented at two pseudo-attP sites, native sequences with partial identity to attP, with one site highly predominant. This study demonstrates in vivo gene transfer in an animal by site-specific genomic integration...
  22. pmc Mutational derivatives of PhiC31 integrase with increased efficiency and specificity
    Annahita Keravala
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305 5120, USA
    Mol Ther 17:112-20. 2009
    ..These mutant integrases are useful for gene therapy and genome modification, and they demonstrate the feasibility of engineering phiC31 integrase toward more desirable properties...
  23. pmc Impact of hydrodynamic injection and phiC31 integrase on tumor latency in a mouse model of MYC-induced hepatocellular carcinoma
    Lauren E Woodard
    Department of Genetics, Stanford University School of Medicine, Stanford, California, United States of America
    PLoS ONE 5:e11367. 2010
    ..However, chromosomal aberrations have been associated with phiC31 integrase expression in tissue culture, leading to questions about safety...
  24. ncbi request reprint Epstein-Barr virus vectors provide prolonged robust factor IX expression in mice
    Christopher R Sclimenti
    Department of Genetics, Stanford University School of Medicine, California 94305, USA
    Biotechnol Prog 19:144-51. 2003
    ..These results underline the crucial importance of appropriate gene expression signals on gene therapy vectors and the utility of EBV sequences in particular for increasing stable gene expression...
  25. doi request reprint The therapeutic potential of ΦC31 integrase as a gene therapy system
    Marisa Karow
    Stanford University School of Medicine, Department of Genetics, Stanford, CA 94305 5120, USA
    Expert Opin Biol Ther 11:1287-96. 2011
    ..Recent progress highlights the unique advantages of this system for in vivo gene therapy and for use in stem cells...
  26. ncbi request reprint Gene transfer to rabbit retina with electron avalanche transfection
    Thomas W Chalberg
    Departments of Genetics, Stanford University, Stanford, CA 94305, USA
    Invest Ophthalmol Vis Sci 47:4083-90. 2006
    ..This study was conducted in the interest of developing efficient and less toxic forms of gene transfer for the eye...
  27. ncbi request reprint Site-specific genomic strategies for gene therapy
    Joylette L Portlock
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    Curr Opin Mol Ther 5:376-82. 2003
    ..We believe that these approaches hold promise for site-specific, safe and efficient gene therapy...
  28. ncbi request reprint Phage phiC31 integrase-mediated genomic integration of the common cytokine receptor gamma chain in human T-cell lines
    Yoshinori Ishikawa
    Department of Microbiology and Immunology, Tohoku University Graduate School of Medicine, 2 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    J Gene Med 8:646-53. 2006
    ..Here, to explore an alternative gene transfer methodology that may offer less risk of insertional mutagenesis, we employed the phiC31 integrase-based integration system using human T-cell lines, including the gammac-deficient ED40515(-)...
  29. pmc Development of a novel helper-dependent adenovirus-Epstein-Barr virus hybrid system for the stable transformation of mammalian cells
    Oliver Dorigo
    Molecular Biology Institute, University of California at Los Angeles, 611 Charles E Young Dr East, Los Angeles, CA 90095 1570, USA
    J Virol 78:6556-66. 2004
    ..This novel gene transfer system has the potential to confer long-term episomal transgene expression and therefore to correct genetic defects with reduced vector-related toxicity and without insertional mutagenesis...
  30. ncbi request reprint Nucleofection of muscle-derived stem cells and myoblasts with phiC31 integrase: stable expression of a full-length-dystrophin fusion gene by human myoblasts
    Simon P Quenneville
    Unité de Recherche en Génétique Humaine, Centre de Recherche du CHUL, CHUQ, Faculte de Medecine, Universite Laval, Sainte Foy, Quebec, Canada, G1V 4G2
    Mol Ther 10:679-87. 2004
    ..A nonviral approach combining nucleofection and the phiC31 integrase may eventually permit safe autotransplantation of genetically modified cells to patients...
  31. ncbi request reprint Site-specific integration for high-level protein production in mammalian cells
    Bhaskar Thyagarajan
    Poetic Genetics, LLC, Burlingame, CA, USA
    Methods Mol Biol 308:99-106. 2005
  32. ncbi request reprint In vivo correction of murine hereditary tyrosinemia type I by phiC31 integrase-mediated gene delivery
    Patrice K Held
    Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239, USA
    Mol Ther 11:399-408. 2005
    ..The stability of transgene expression, relatively high integration frequency, and significant site specificity that characterize the phiC31 integration system suggest that it may have utility in many gene therapy settings...

Research Grants17

  1. Retinal Gene Therapy by Site-Specific Integration
    MICHELE CALOS; Fiscal Year: 2006
    ..These experiments will move the phiC31 integrase ..
  2. Transferring integrase technology to animals
    MICHELE CALOS; Fiscal Year: 2006
    ..By scaling up to animals more similar to humans, these experiments will move the site-specific integrase technology closer to the clinic. ..
  3. Custom integration tools for functional genomics
    MICHELE CALOS; Fiscal Year: 2003
    ..We develop the appropriate DNA shuffling and genetic screening methods here for generation of a library of custom integration tools. The strategy is scalable to reach essentially all genes in the mouse genome. ..
  4. Transferring integrase technology to animals
    MICHELE CALOS; Fiscal Year: 2003
    ..Success in these experiments will lead to application of this innovative technology to other gene therapy settings and progress toward clinical trials in patients. ..
  5. SITE-SPECIFIC INTEGRATION FOR GENE THERAPY
    MICHELE CALOS; Fiscal Year: 2003
    ..The novel site-specific integration strategy developed here can be used to add value to most current gene therapy vectors. ..