Axel T Brunger

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. Brunger A, Choi U, Lai Y, Leitz J, Zhou Q. Molecular Mechanisms of Fast Neurotransmitter Release. Annu Rev Biophys. 2018;47:469-497 pubmed publisher
    ..These studies will be a stepping-stone toward answering the question of how the synaptic vesicle fusion machinery achieves such high speed and sensitivity. ..
  2. Lai Y, Choi U, Zhang Y, Zhao M, Pfuetzner R, Wang A, et al. N-terminal domain of complexin independently activates calcium-triggered fusion. Proc Natl Acad Sci U S A. 2016;113:E4698-707 pubmed publisher
    ..Membrane binding of the N-terminal domain of complexin therefore cooperates with the other fusogenic elements of the synaptic fusion machinery during Ca(2+)-triggered release. ..
  3. Brunger A, Choi U, Lai Y, Leitz J, White K, Zhou Q. The pre-synaptic fusion machinery. Curr Opin Struct Biol. 2019;54:179-188 pubmed publisher
    ..These structures show how this machinery captures the SNARE substrate and provide clues as to a possible processing mechanism. ..
  4. Brunger A, Jin R, Breidenbach M. Highly specific interactions between botulinum neurotoxins and synaptic vesicle proteins. Cell Mol Life Sci. 2008;65:2296-306 pubmed publisher
    ..In this review we discuss the structural basis for botulinum toxin's exquisite specificity for its neuronal cell-surface receptors and intracellular SNARE targets. ..
  5. Brunger A, Leitz J, Zhou Q, Choi U, Lai Y. Ca2+-Triggered Synaptic Vesicle Fusion Initiated by Release of Inhibition. Trends Cell Biol. 2018;28:631-645 pubmed publisher
  6. Brunger A, Rummel A. Receptor and substrate interactions of clostridial neurotoxins. Toxicon. 2009;54:550-60 pubmed publisher
    ..After its translocation the enzymatically active light chain specifically hydrolyses specific SNARE proteins, preventing SNARE complex assembly and thereby blocking exocytosis of neurotransmitter. ..
  7. Brunger A, Adams P, Fromme P, Fromme R, Levitt M, Schröder G. Improving the accuracy of macromolecular structure refinement at 7 Å resolution. Structure. 2012;20:957-66 pubmed publisher
    ..We also found a significant correlation between R(free) values and the accuracy of the model, suggesting that R(free) is useful even at low resolution. ..
  8. Brunger A, DeLaBarre B, Davies J, Weis W. X-ray structure determination at low resolution. Acta Crystallogr D Biol Crystallogr. 2009;65:128-33 pubmed publisher
    ..It is concluded that de novo model building is problematic at low resolution and refinement should start from high-resolution crystal structures whenever possible. ..
  9. Zhao M, Brunger A. Recent Advances in Deciphering the Structure and Molecular Mechanism of the AAA+ ATPase N-Ethylmaleimide-Sensitive Factor (NSF). J Mol Biol. 2016;428:1912-26 pubmed publisher
    ..We also compare NSF with other known AAA+ family members. ..

More Information

Publications14

  1. Brunger A, Das D, Deacon A, Grant J, Terwilliger T, Read R, et al. Application of DEN refinement and automated model building to a difficult case of molecular-replacement phasing: the structure of a putative succinyl-diaminopimelate desuccinylase from Corynebacterium glutamicum. Acta Crystallogr D Biol Crystallogr. 2012;68:391-403 pubmed publisher
    ..This example also illustrates a current limitation of automated procedures that require manual adjustment of local sequence misalignments between the homology model and the target sequence. ..
  2. Lai Y, Diao J, Cipriano D, Zhang Y, Pfuetzner R, Padolina M, et al. Complexin inhibits spontaneous release and synchronizes Ca2+-triggered synaptic vesicle fusion by distinct mechanisms. elife. 2014;3:e03756 pubmed publisher
    ..Taken together, complexin-1 utilizes distinct mechanisms for synchronization of Ca(2+)-triggered fusion and inhibition of spontaneous fusion. ..
  3. Ressl S, Vu B, Vivona S, Martinelli D, Südhof T, Brunger A. Structures of C1q-like proteins reveal unique features among the C1q/TNF superfamily. Structure. 2015;23:688-99 pubmed publisher
    ..Our results reveal unique structural features of C1QLs among C1q/TNF superfamily proteins that may be associated with their specific brain functions. ..
  4. Gipson P, Fukuda Y, Danev R, Lai Y, Chen D, Baumeister W, et al. Morphologies of synaptic protein membrane fusion interfaces. Proc Natl Acad Sci U S A. 2017;114:9110-9115 pubmed publisher
    ..We conclude that synaptic proteins have evolved to limit possible contact site assemblies and morphologies to those that promote fast Ca2+-triggered release. ..
  5. Lai Y, Choi U, Leitz J, Rhee H, Lee C, Altas B, et al. Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18. Neuron. 2017;95:591-607.e10 pubmed publisher
    ..Together, the physiological functions of Munc13 may be related to regulation of proper SNARE complex assembly. ..