Steven Block

Summary

Affiliation: Stanford University
Country: USA

Publications

  1. pmc Direct observation of base-pair stepping by RNA polymerase
    Elio A Abbondanzieri
    Department of Applied Physics, Stanford University, Stanford, California 94305, USA
    Nature 438:460-5. 2005
  2. pmc Backsteps induced by nucleotide analogs suggest the front head of kinesin is gated by strain
    Nicholas R Guydosh
    Biophysics Program and Departments of Biological Sciences and Applied Physics, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 103:8054-9. 2006
  3. pmc Not so lame after all: kinesin still walks with a hobbled head
    Nicholas R Guydosh
    Biophysics Program, Stanford University, Stanford, CA 94305, USA
    J Gen Physiol 130:441-4. 2007
  4. pmc A universal pathway for kinesin stepping
    Bason E Clancy
    Department of Biology, Stanford University, Stanford, California, USA
    Nat Struct Mol Biol 18:1020-7. 2011
  5. pmc Combined optical trapping and single-molecule fluorescence
    Matthew J Lang
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    J Biol 2:6. 2003
  6. pmc Molecule by molecule, the physics and chemistry of life: SMB 2007
    Steven M Block
    Department of Applied Physics, Stanford University, Stanford, California 94305, USA
    Nat Chem Biol 3:193-7. 2007
  7. pmc Probing the kinesin reaction cycle with a 2D optical force clamp
    Steven M Block
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 100:2351-6. 2003
  8. pmc Direct measurements of kinesin torsional properties reveal flexible domains and occasional stalk reversals during stepping
    Braulio Gutiérrez-Medina
    Department of Biology, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 106:17007-12. 2009
  9. pmc Individual dimers of the mitotic kinesin motor Eg5 step processively and support substantial loads in vitro
    Megan T Valentine
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Nat Cell Biol 8:470-6. 2006
  10. pmc Direct observation of the binding state of the kinesin head to the microtubule
    Nicholas R Guydosh
    Biophysics Program, Stanford University, Stanford, California 94305, USA
    Nature 461:125-8. 2009

Research Grants

Collaborators

Detail Information

Publications28

  1. pmc Direct observation of base-pair stepping by RNA polymerase
    Elio A Abbondanzieri
    Department of Applied Physics, Stanford University, Stanford, California 94305, USA
    Nature 438:460-5. 2005
    ..Global fits were inconsistent with a model for movement incorporating a power stroke tightly coupled to pyrophosphate release, but consistent with a brownian ratchet model incorporating a secondary NTP binding site...
  2. pmc Backsteps induced by nucleotide analogs suggest the front head of kinesin is gated by strain
    Nicholas R Guydosh
    Biophysics Program and Departments of Biological Sciences and Applied Physics, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 103:8054-9. 2006
    ..Together, our results support stepping models in which ATP binding triggers the mechanical step and the front head is gated by strain...
  3. pmc Not so lame after all: kinesin still walks with a hobbled head
    Nicholas R Guydosh
    Biophysics Program, Stanford University, Stanford, CA 94305, USA
    J Gen Physiol 130:441-4. 2007
  4. pmc A universal pathway for kinesin stepping
    Bason E Clancy
    Department of Biology, Stanford University, Stanford, California, USA
    Nat Struct Mol Biol 18:1020-7. 2011
    ..In addition to inter-head tension, we found that neck-linker orientation is also responsible for ensuring gating in kinesin...
  5. pmc Combined optical trapping and single-molecule fluorescence
    Matthew J Lang
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    J Biol 2:6. 2003
    ..Here, we report the development of an instrument capable of true, simultaneous, spatially coincident optical trapping and single-molecule fluorescence...
  6. pmc Molecule by molecule, the physics and chemistry of life: SMB 2007
    Steven M Block
    Department of Applied Physics, Stanford University, Stanford, California 94305, USA
    Nat Chem Biol 3:193-7. 2007
    ..Interdisciplinary work in the life sciences at the boundaries of biology, chemistry and physics is making enormous strides. This progress was showcased at the recent Single Molecule Biophysics conference...
  7. pmc Probing the kinesin reaction cycle with a 2D optical force clamp
    Steven M Block
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 100:2351-6. 2003
    ..Fluctuation analysis shows that the cycle contains at least four transitions, and confirms that hydrolysis remains tightly coupled to stepping. Together, our findings pose challenges for models of kinesin motion...
  8. pmc Direct measurements of kinesin torsional properties reveal flexible domains and occasional stalk reversals during stepping
    Braulio Gutiérrez-Medina
    Department of Biology, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 106:17007-12. 2009
    ..Our results impose constraints on kinesin walking models and suggest a role for rotational freedom in cargo transport...
  9. pmc Individual dimers of the mitotic kinesin motor Eg5 step processively and support substantial loads in vitro
    Megan T Valentine
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Nat Cell Biol 8:470-6. 2006
    ..The Eg5-513-5His velocity data were described by a minimal, three-state model where a force-dependent transition follows nucleotide binding...
  10. pmc Direct observation of the binding state of the kinesin head to the microtubule
    Nicholas R Guydosh
    Biophysics Program, Stanford University, Stanford, California 94305, USA
    Nature 461:125-8. 2009
    ..The linked head could only rebind the microtubule once ATP had become bound to its partner head...
  11. pmc Kinesin moves by an asymmetric hand-over-hand mechanism
    Charles L Asbury
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Science 302:2130-4. 2003
    ..Limping implies that kinesin molecules strictly alternate between two different conformations as they step, indicative of an asymmetric, hand-over-hand mechanism...
  12. pmc An automated two-dimensional optical force clamp for single molecule studies
    Matthew J Lang
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    Biophys J 83:491-501. 2002
    ..We present details of force clamp operation and preliminary data showing kinesin motor movement subject to diagonal and forward loads...
  13. pmc Single-molecule studies of RNA polymerase: motoring along
    Kristina M Herbert
    Biophysics Program, Stanford University, Stanford, CA 94305, USA
    Annu Rev Biochem 77:149-76. 2008
    ..We discuss recent progress achieved by single-molecule studies, highlighting some of the unresolved questions and ongoing debates...
  14. pmc Precision steering of an optical trap by electro-optic deflection
    Megan T Valentine
    Department of Biology, Stanford University, Stanford, California 94305, USA
    Opt Lett 33:599-601. 2008
    ..These attributes impart improved force and position resolution, making EOD-based traps a promising alternative for high-precision nanomechanical measurements of biomaterials...
  15. pmc Forward and reverse motion of single RecBCD molecules on DNA
    Thomas T Perkins
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    Biophys J 86:1640-8. 2004
    ..Our observations show that RecBCD-DNA complexes can exist in multiple, functionally distinct states that persist for many catalytic turnovers: such states may help tune enzyme activity for various biological functions...
  16. pmc Sequence-dependent pausing of single lambda exonuclease molecules
    Thomas T Perkins
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Science 301:1914-8. 2003
    ..This sequence is found in the left lambda cohesive end, where exonuclease inhibition may contribute to the reduced recombination efficiency at that end...
  17. ncbi request reprint Ubiquitous transcriptional pausing is independent of RNA polymerase backtracking
    Keir C Neuman
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Cell 115:437-47. 2003
    ..We propose instead that they are caused by a structural rearrangement within the enzyme...
  18. pmc Statistical kinetics of macromolecular dynamics
    Joshua W Shaevitz
    Departments of Physics, Biological Sciences, and Applied Physics, Stanford University, Stanford, CA 94305 5020, USA
    Biophys J 89:2277-85. 2005
    ....
  19. pmc Applied force reveals mechanistic and energetic details of transcription termination
    Matthew H Larson
    Biophysics Program, Stanford University, Stanford, CA 94305, USA
    Cell 132:971-82. 2008
    ..We propose a quantitative, energetic model that predicts the behavior for these terminators and mutant variants...
  20. pmc On the origin of kinesin limping
    Adrian N Fehr
    Department of Applied Physics, Stanford University, Stanford, California, USA
    Biophys J 97:1663-70. 2009
    ..These results suggest that kinesin heads take different vertical trajectories during alternate steps, and that the rates for these motions are differentially sensitive to load...
  21. pmc High-resolution, single-molecule measurements of biomolecular motion
    William J Greenleaf
    Department of Applied Physics, Stanford University, Stanford, California 94305 5030, USA
    Annu Rev Biophys Biomol Struct 36:171-90. 2007
    ..Extensions to these methods are also discussed, with an eye toward future application to outstanding biological problems...
  22. pmc Kinesin motor mechanics: binding, stepping, tracking, gating, and limping
    Steven M Block
    Department of Biological Sciences and Department of Applied Physics, Stanford University, Stanford, California 94305, USA
    Biophys J 92:2986-95. 2007
  23. pmc Single-molecule, motion-based DNA sequencing using RNA polymerase
    William J Greenleaf
    Department of Applied Physics, Stanford University, Stanford, CA 94305, USA
    Science 313:801. 2006
    ..This proof of principle demonstrates that the motion of a processive nucleic acid enzyme may be used to extract sequence information directly from DNA...
  24. pmc Pulling on the nascent RNA during transcription does not alter kinetics of elongation or ubiquitous pausing
    Ravindra V Dalal
    Department of Physics, Stanford University, Stanford, California 94305, USA
    Mol Cell 23:231-9. 2006
    ..The ability of the TEC to sustain large loads on the transcript reflects a tight binding of RNA within the TEC and has important implications for models of transcriptional termination...
  25. pmc Stepping and stretching. How kinesin uses internal strain to walk processively
    Steven S Rosenfeld
    Department of Neurology, University of Alabama at Birmingham, Birmingham, Alabama 35294 3293, USA
    J Biol Chem 278:18550-6. 2003
    ..This effect guarantees the two motor domains remain out of phase for many mechanochemical cycles and provides an efficient and adaptable mechanism for the maintenance of processive movement...
  26. pmc The importance of lattice defects in katanin-mediated microtubule severing in vitro
    Liza J Davis
    Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Biophys J 82:2916-27. 2002
    ..As a result of our analysis, we propose that defects in the microtubule lattice, which are known to exist but previously not known to have any biological function, serve as sites for katanin activity...
  27. pmc Kinesin steps do not alternate in size
    Adrian N Fehr
    Biophys J 94:L20-2. 2008
    ..In both cases, we find that kinesin takes uniform 8-nm steps, a result that strongly constrains the allowed models...
  28. pmc Direct measurement of the full, sequence-dependent folding landscape of a nucleic acid
    Michael T Woodside
    National Institute for Nanotechnology, National Research Council of Canada, Edmonton AB, Canada, T6G 2M9
    Science 314:1001-4. 2006
    ....

Research Grants21

  1. Nucleic acid enzymes studied at the molecular level
    Steven Block; Fiscal Year: 2005
    ..abstract_text> ..
  2. MICROTUBULE MOTORS STUDIED ON A MOLECULAR SCALE
    Steven Block; Fiscal Year: 2006
    ..For this purpose, advanced instrumentation based on optical trapping ('optical tweezers') and optical nanometry has been developed, and will be used in experiments conducted at the single-molecule level. ..
  3. MICROTUBULE MOTORS STUDIED ON A MOLECULAR SCALE
    Steven Block; Fiscal Year: 2005
    ..For this purpose, advanced instrumentation based on optical trapping ('optical tweezers') and optical nanometry has been developed, and will be used in experiments conducted at the single-molecule level. ..
  4. MICROTUBULE MOTORS STUDIED ON A MOLECULAR SCALE
    Steven Block; Fiscal Year: 2004
    ..For this purpose, advanced instrumentation based on optical trapping ('optical tweezers') and optical nanometry has been developed, and will be used in experiments conducted at the single-molecule level. ..
  5. Nucleic acid enzymes studied at the molecular level
    Steven Block; Fiscal Year: 2004
    ..abstract_text> ..
  6. MICROTUBULE MOTORS STUDIED ON A MOLECULAR SCALE
    Steven Block; Fiscal Year: 2003
    ..For this purpose, advanced instrumentation based on optical trapping ('optical tweezers') and optical nanometry has been developed, and will be used in experiments conducted at the single-molecule level. ..
  7. MICROTUBULE MOTORS STUDIED ON A MOLECULAR SCALE
    Steven Block; Fiscal Year: 2002
    ..For this purpose, advanced instrumentation based on optical trapping ('optical tweezers') and optical nanometry has been developed, and will be used in experiments conducted at the single-molecule level. ..
  8. Nucleic acid enzymes studied at the molecular level
    Steven Block; Fiscal Year: 2009
    ..4A). A direct demonstration that RNAP advances in single basepair increments would allow us to rule out a competing theory that it moves by a so-called "inchworm" mechanism. ..
  9. MICROTUBULE MOTORS STUDIES ON A MOLECULAR SCALE
    Steven Block; Fiscal Year: 2001
    ..For this purpose, advanced instrumentation based on optical trapping (optical tweezers) and optical nanometry has been developed, and will be used in experiments at the single molecule level. ..
  10. MICROTUBULE MOTORS STUDIED ON A MOLECULAR SCALE
    Steven Block; Fiscal Year: 2009
    ....
  11. MICROTUBULE MOTORS STUDIES ON A MOLECULAR SCALE
    Steven Block; Fiscal Year: 2000
    ..For this purpose, advanced instrumentation based on optical trapping (optical tweezers) and optical nanometry has been developed, and will be used in experiments at the single molecule level. ..
  12. MICROTUBULE MOTORS STUDIED ON A MOLECULAR SCALE
    Steven Block; Fiscal Year: 2007
    ....
  13. Nucleic acid enzymes studied at the molecular level
    Steven Block; Fiscal Year: 2007
    ..4Angstroms). A direct demonstration that RNAP advances in single basepair increments would allow us to rule out a competing theory that it moves by a so-called "inchworm" mechanism. ..
  14. Nucleic acid enzymes studied at the molecular level
    Steven Block; Fiscal Year: 2006
    ..4Angstroms). A direct demonstration that RNAP advances in single basepair increments would allow us to rule out a competing theory that it moves by a so-called "inchworm" mechanism. ..
  15. MICROTUBULE MOTORS STUDIES ON A MOLECULAR SCALE
    Steven Block; Fiscal Year: 1999
    ..For this purpose, advanced instrumentation based on optical trapping (optical tweezers) and optical nanometry has been developed, and will be used in experiments at the single molecule level. ..
  16. Nucleic acid enzymes studied at the molecular level
    Steven Block; Fiscal Year: 2003
    ..abstract_text> ..
  17. Nucleic acid enzymes studied at the molecular level
    Steven Block; Fiscal Year: 2002
    ..abstract_text> ..
  18. TRANSCRIPTION STUDIED AT THE MOLECULAR LEVEL
    Steven Block; Fiscal Year: 2000
    ....
  19. MICROTUBULE MOTORS STUDIED ON A MOLECULAR SCALE
    Steven M Block; Fiscal Year: 2010
    ....