Paul E Mead

Summary

Affiliation: St. Jude Children's Research Hospital
Country: USA

Publications

  1. pmc Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis
    Donald A Yergeau
    Department of Pathology, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA
    Mob DNA 2:15. 2011
  2. pmc Manipulating the Xenopus genome with transposable elements
    Donald A Yergeau
    Department of Pathology, St, Jude Children s Research Hospital, North Lauderdale Street, Memphis, Tennessee 38105, USA
    Genome Biol 8:S11. 2007
  3. ncbi request reprint Evi-1 expression in Xenopus
    Paul E Mead
    Department of Pathology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Gene Expr Patterns 5:601-8. 2005
  4. pmc CDK9/cyclin complexes modulate endoderm induction by direct interaction with Mix.3/mixer
    Haiqing Zhu
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    Dev Dyn 238:1346-57. 2009
  5. pmc Transposon transgenesis in Xenopus
    Donald A Yergeau
    Department of Pathology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Methods 51:92-100. 2010
  6. pmc Remobilization of Tol2 transposons in Xenopus tropicalis
    Donald A Yergeau
    Department of Pathology, St, Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA
    BMC Dev Biol 10:11. 2010
  7. pmc Transgenesis in Xenopus using the Sleeping Beauty transposon system
    Donald A Yergeau
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Dev Dyn 238:1727-43. 2009
  8. ncbi request reprint Injection-mediated transposon transgenesis in Xenopus tropicalis and the identification of integration sites by modified extension primer tag selection (EPTS) linker-mediated PCR
    Donald A Yergeau
    Department of Pathology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, Tennessee 38105, USA
    Nat Protoc 2:2975-86. 2007
  9. ncbi request reprint Cloning and expression pattern of the Xenopus erythropoietin receptor
    Donald A Yergeau
    Department of Pathology, St Jude Children s Research Hospital, 332 North Lauderdale St, Memphis, TN 38105, USA
    Gene Expr Patterns 6:420-5. 2006
  10. doi request reprint Xenopus transgenics: methods using transposons
    Clair M Kelley
    Department of Pathology, St Jude Children s Research Hospital, Memphis, TN, USA
    Methods Mol Biol 917:231-43. 2012

Collaborators

Detail Information

Publications16

  1. pmc Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis
    Donald A Yergeau
    Department of Pathology, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA
    Mob DNA 2:15. 2011
    ..abstract:..
  2. pmc Manipulating the Xenopus genome with transposable elements
    Donald A Yergeau
    Department of Pathology, St, Jude Children s Research Hospital, North Lauderdale Street, Memphis, Tennessee 38105, USA
    Genome Biol 8:S11. 2007
    ....
  3. ncbi request reprint Evi-1 expression in Xenopus
    Paul E Mead
    Department of Pathology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Gene Expr Patterns 5:601-8. 2005
    ..Expression of Evi-1 is detected by in situ hybridization in the pronephric tissue, the brain and in neural crest derivatives of the head and neck...
  4. pmc CDK9/cyclin complexes modulate endoderm induction by direct interaction with Mix.3/mixer
    Haiqing Zhu
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    Dev Dyn 238:1346-57. 2009
    ..3 during embryonic development. Developmental Dynamics 238:1346-1357, 2009. (c) 2009 Wiley-Liss, Inc...
  5. pmc Transposon transgenesis in Xenopus
    Donald A Yergeau
    Department of Pathology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Methods 51:92-100. 2010
    ....
  6. pmc Remobilization of Tol2 transposons in Xenopus tropicalis
    Donald A Yergeau
    Department of Pathology, St, Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA
    BMC Dev Biol 10:11. 2010
    ..Tol2 transposons are stable in the frog genome and are transmitted to the offspring at the expected Mendelian frequency...
  7. pmc Transgenesis in Xenopus using the Sleeping Beauty transposon system
    Donald A Yergeau
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Dev Dyn 238:1727-43. 2009
    ..tropicalis genome by a noncanonical process and are passed through the germline. We compare the activity of SB in this model organism with that of Tol2, a hAT (hobo, Ac1, TAM)-like transposon system...
  8. ncbi request reprint Injection-mediated transposon transgenesis in Xenopus tropicalis and the identification of integration sites by modified extension primer tag selection (EPTS) linker-mediated PCR
    Donald A Yergeau
    Department of Pathology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, Tennessee 38105, USA
    Nat Protoc 2:2975-86. 2007
    ..This cloning strategy allows rapid identification of genomic sequences flanking the integration sites and multiple independently segregating transposon integration events in a single tadpole can be cloned simultaneously...
  9. ncbi request reprint Cloning and expression pattern of the Xenopus erythropoietin receptor
    Donald A Yergeau
    Department of Pathology, St Jude Children s Research Hospital, 332 North Lauderdale St, Memphis, TN 38105, USA
    Gene Expr Patterns 6:420-5. 2006
    ..Expression of the erythropoietin receptor is first detected by in situ hybridization in the ventral blood island during tailbud stages...
  10. doi request reprint Xenopus transgenics: methods using transposons
    Clair M Kelley
    Department of Pathology, St Jude Children s Research Hospital, Memphis, TN, USA
    Methods Mol Biol 917:231-43. 2012
    ..The cellular machinery rapidly translates the exogenous mRNA to produce active transposase enzyme that catalyzes excision of the transposon substrate from the plasmid and stable integration into the genomic DNA...
  11. ncbi request reprint A flk-1 promoter/enhancer reporter transgenic Xenopus laevis generated using the Sleeping Beauty transposon system: an in vivo model for vascular studies
    Joanne R Doherty
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Dev Dyn 236:2808-17. 2007
    ..These studies demonstrate the convenience of the SB system for generating transgenic animals and the utility of the xflk-1:GFP transgenic line for in vivo studies of vascular development...
  12. ncbi request reprint Tol2 transposon-mediated transgenesis in Xenopus tropicalis
    Michelle R Johnson Hamlet
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Genesis 44:438-45. 2006
    ..Here, we show that the Medaka fish transposon, Tol2, is able to stably integrate into the X. tropicalis genome and will serve as a powerful tool for insertional mutagenesis strategies in the frog...
  13. doi request reprint Forward genetic screens in Xenopus using transposon-mediated insertional mutagenesis
    Donald A Yergeau
    Department of Pathology, St Jude Children s Research Hospital, Memphis, TN, USA
    Methods Mol Biol 917:111-27. 2012
    ..As each outcross of Xenopus tropicalis typically produces 2,000, or more, progeny, this method can be used to perform large-scale insertional mutagenesis screens in this highly tractable developmental model system...
  14. ncbi request reprint Determination of the minimal domains of Mix.3/Mixer required for endoderm development
    Joanne R Doherty
    Department of Pathology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105, USA
    Mech Dev 123:56-66. 2006
    ..3/Mixer that confer endoderm-inducing activity. These studies reveal a novel co-operation between the homeodomain and a small domain in the carboxyl terminal region that is essential for Mix.3/Mixer function...
  15. ncbi request reprint Expression of Xenopus suppressor of cytokine signaling 3 (xSOCS3) is induced by epithelial wounding
    Emin Kuliyev
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Dev Dyn 233:1123-30. 2005
    ....
  16. ncbi request reprint BMP4-dependent expression of Xenopus Grainyhead-like 1 is essential for epidermal differentiation
    Jianning Tao
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Development 132:1021-34. 2005
    ..These results establish a novel developmental role for XGrhl1 as a crucial tissue-specific regulator of vertebrate epidermal differentiation...

Research Grants8

  1. Insertional Mutagenesis Strategies in Xenopus tropicalis
    Paul Mead; Fiscal Year: 2006
    ..The proposed study will provide valuable insight into the biology of hematopoiesis in both normal and disease states. ..
  2. Tol2-mediated gene and enhancer trapping in Xenopus tropicalis
    Paul Mead; Fiscal Year: 2009
    ..We are currently characterizing the novel integration events in the progeny from the other remobilized F2 frogs. We anticipate submitting a manuscript describing this data soon. ..
  3. Tol2-mediated gene and enhancer trapping in Xenopus tropicalis
    Paul E Mead; Fiscal Year: 2010
    ..We are currently characterizing the novel integration events in the progeny from the other remobilized F2 frogs. We anticipate submitting a manuscript describing this data soon. ..