S Jackowski

Summary

Affiliation: St. Jude Children's Research Hospital
Country: USA

Publications

  1. ncbi request reprint Activity of the phosphatidylcholine biosynthetic pathway modulates the distribution of fatty acids into glycerolipids in proliferating cells
    S Jackowski
    Department of Biochemistry St Jude Children s Research Hospital, 332 North Lauderdale, Memphis, TN 38105 2794, USA
    Biochim Biophys Acta 1483:301-15. 2000
  2. pmc Disruption of CCTbeta2 expression leads to gonadal dysfunction
    Suzanne Jackowski
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105 2794, USA
    Mol Cell Biol 24:4720-33. 2004
  3. ncbi request reprint CTP: Phosphocholine cytidylyltransferase: paving the way from gene to membrane
    Suzanne Jackowski
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 280:853-6. 2005
  4. ncbi request reprint Distribution of CTP:phosphocholine cytidylyltransferase (CCT) isoforms. Identification of a new CCTbeta splice variant
    A Lykidis
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 274:26992-7001. 1999
  5. ncbi request reprint Pantothenate kinase regulation of the intracellular concentration of coenzyme A
    C O Rock
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 275:1377-83. 2000
  6. pmc The licC gene of Streptococcus pneumoniae encodes a CTP:phosphocholine cytidylyltransferase
    C O Rock
    Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
    J Bacteriol 183:4927-31. 2001
  7. ncbi request reprint 2-Acylglycerolphosphoethanolamine acyltransferase/acyl-acyl carrier protein synthetase is a membrane-associated acyl carrier protein binding protein
    C L Cooper
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101
    J Biol Chem 264:7384-9. 1989
  8. ncbi request reprint Lipid activation of CTP: phosphocholine cytidylyltransferase alpha: characterization and identification of a second activation domain
    A Lykidis
    Department of Biochemistry, St. Jude Children's Research Hospital, Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
    Biochemistry 40:494-503. 2001
  9. ncbi request reprint Role of calcium-independent phospholipases (iPLA(2)) in phosphatidylcholine metabolism
    C H Chiu
    Protein Science Division, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
    Biochem Biophys Res Commun 287:600-6. 2001
  10. pmc Overproduction of beta-ketoacyl-acyl carrier protein synthase I imparts thiolactomycin resistance to Escherichia coli K-12
    J T Tsay
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101
    J Bacteriol 174:508-13. 1992

Research Grants

  1. Regulation of Coenzyme A Levels by Panothenate Kinase
    Suzanne Jackowski; Fiscal Year: 2010
  2. Regulation of Coenzyme A Levels by Pantothenate Kinase
    Suzanne Jackowski; Fiscal Year: 2005
  3. REGULATION OF PHOSPHOLIPID SYNTHESIS
    Suzanne Jackowski; Fiscal Year: 2006
  4. Regulation of Coenzyme A Levels by Panothenate Kinase
    Suzanne Jackowski; Fiscal Year: 2007
  5. Regulation of Coenzyme A Levels by Panothenate Kinase
    Suzanne Jackowski; Fiscal Year: 2009
  6. REGULATION OF MEMBRANE PHOSPHOLIPID SYNTHESIS
    Suzanne Jackowski; Fiscal Year: 2002
  7. REGULATION OF MEMBRANE BIOSYNTHESIS BY CSF-1
    Suzanne Jackowski; Fiscal Year: 1993

Collaborators

  • J E Cronan
  • C O Rock
  • J Wang
  • Shigeru Chohnan
  • R S Williams
  • C H Chiu
  • Yong Mei Zhang
  • W J Song
  • J R Kinghorn
  • S E Unkles
  • R J Heath
  • W Yang
  • E I Tuomanen
  • Joerg R Weber
  • STEPHEN W contact WHITE
  • Roberta Leonardi
  • A Lykidis
  • Christopher Gunter
  • Yong Tian
  • Rungtawan Sriburi
  • Paolo Fagone
  • Joseph W Brewer
  • Kristopher G Virga
  • Hee Won Park
  • Jerold E Rehg
  • Matthew Frank
  • Richard E Lee
  • Robert A Ivey
  • Nathan I Nicely
  • Bum Soo Hong
  • K Gopal Murti
  • Kirk Hevener
  • Limin Wang
  • Janine Zweigner
  • Gayathri Ramaswamy
  • Mohammad Karim
  • Belen Ramos
  • Bo Yeon Kwak
  • M Yun
  • Matthew W Frank
  • Kathryn E Gunn
  • Pamela D Jackson
  • Christopher R Sage
  • R B Calder
  • Athanasios Lykidis
  • Al Claiborne
  • Gregory R Robbins
  • Hemamalini Bommiasamy
  • Gerald L Buldak
  • Cheryl Ward-Chapman
  • Gerald L Newton
  • Robert C Fahey
  • Carleitta Paige
  • T Conn Mallett
  • Masoud Vedadi
  • Ruobing Zhou
  • Guillermo Senisterra
  • Wael M Rabeh
  • Derek Parsonage
  • Pamela Jackson
  • Ira Tabas
  • Susan Magdaleno
  • Shannon H Smith
  • Marie van der Merwe
  • Mohammad A Karim
  • Kazutoshi Mori
  • Pam Jackson
  • Mohammed El Mouedden
  • Mikyung Yun
  • Enrique Claro
  • M A Karim
  • P Jackson
  • X X Xu
  • H W Park
  • C G Park
  • J Y Kim
  • I Baburina
  • E Campbell
  • G Ramaswamy
  • K G Murti
  • J T Tsay
  • P D Jackson
  • M S Rutherford
  • N A Jenkins
  • T G Tessner
  • N G Copeland
  • L Hsu
  • D J Gilbert
  • C L Cooper
  • T J Larson

Detail Information

Publications55

  1. ncbi request reprint Activity of the phosphatidylcholine biosynthetic pathway modulates the distribution of fatty acids into glycerolipids in proliferating cells
    S Jackowski
    Department of Biochemistry St Jude Children s Research Hospital, 332 North Lauderdale, Memphis, TN 38105 2794, USA
    Biochim Biophys Acta 1483:301-15. 2000
    ..Thus, the cellular DG supply did not regulate PtdCho biosynthesis and CCT activity governs the partitioning of DG into either the PL or TG pools, thereby controlling both PtdCho and TG biosynthesis...
  2. pmc Disruption of CCTbeta2 expression leads to gonadal dysfunction
    Suzanne Jackowski
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105 2794, USA
    Mol Cell Biol 24:4720-33. 2004
    ..The results indicate that although CCTbeta2 is expressed at very low levels compared to the alpha-isoform, loss of CCTbeta2 expression causes a breakdown in the gonadal response to hormonal stimulation...
  3. ncbi request reprint CTP: Phosphocholine cytidylyltransferase: paving the way from gene to membrane
    Suzanne Jackowski
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 280:853-6. 2005
  4. ncbi request reprint Distribution of CTP:phosphocholine cytidylyltransferase (CCT) isoforms. Identification of a new CCTbeta splice variant
    A Lykidis
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 274:26992-7001. 1999
    ....
  5. ncbi request reprint Pantothenate kinase regulation of the intracellular concentration of coenzyme A
    C O Rock
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 275:1377-83. 2000
    ..These data support the concept that the expression and/or activity of PanK is a determining factor in the physiological regulation of the intracellular CoA concentration...
  6. pmc The licC gene of Streptococcus pneumoniae encodes a CTP:phosphocholine cytidylyltransferase
    C O Rock
    Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
    J Bacteriol 183:4927-31. 2001
    ..Phosphoethanolamine is a poor substrate. The LicC protein plays a role in the biosynthesis of the phosphocholine-derivatized cell wall constituents that are critical for cell separation and pathogenesis...
  7. ncbi request reprint 2-Acylglycerolphosphoethanolamine acyltransferase/acyl-acyl carrier protein synthetase is a membrane-associated acyl carrier protein binding protein
    C L Cooper
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101
    J Biol Chem 264:7384-9. 1989
    ..These data indicate that the 2-acyl-GPE acyltransferase and acyl-ACP synthetase reactions are catalyzed by the same membrane protein that possesses a high affinity binding site for soluble ACP...
  8. ncbi request reprint Lipid activation of CTP: phosphocholine cytidylyltransferase alpha: characterization and identification of a second activation domain
    A Lykidis
    Department of Biochemistry, St. Jude Children's Research Hospital, Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
    Biochemistry 40:494-503. 2001
    ..The data also demonstrate for the first time that the 57-residue carboxy-terminal domain in CCTalpha participates in lipid-mediated regulation and is sufficient for maximum activation of enzyme activity...
  9. ncbi request reprint Role of calcium-independent phospholipases (iPLA(2)) in phosphatidylcholine metabolism
    C H Chiu
    Protein Science Division, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
    Biochem Biophys Res Commun 287:600-6. 2001
    ..Since iPLA(2) overexpression did not accelerate either phospholipid remodeling or phosphatidylcholine synthesis, this enzyme does play a determinant (rate-controlling?) role in either of these cellular processes...
  10. pmc Overproduction of beta-ketoacyl-acyl carrier protein synthase I imparts thiolactomycin resistance to Escherichia coli K-12
    J T Tsay
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101
    J Bacteriol 174:508-13. 1992
    ..However, extracts from a TLM-resistant mutant (strain CDM5) contained normal levels of TLM-sensitive synthase I activity, illustrating that there are other mechanisms of TLM resistance...
  11. ncbi request reprint Cloning and characterization of a second human CTP:phosphocholine cytidylyltransferase
    A Lykidis
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 273:14022-9. 1998
    ..These data demonstrate that a second, distinct CCT enzyme is expressed in human tissues and provides another mechanism by which cells regulate phosphatidylcholine production...
  12. ncbi request reprint Overexpression of a mammalian ethanolamine-specific kinase accelerates the CDP-ethanolamine pathway
    A Lykidis
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 276:2174-9. 2001
    ..The data demonstrate the existence of separate ethanolamine and choline kinases in mammals and show that ethanolamine kinase can be a rate-controlling step in PtdEtn biosynthesis...
  13. ncbi request reprint Kinetics and regulation of pantothenate kinase from Escherichia coli
    W J Song
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101
    J Biol Chem 269:27051-8. 1994
    ..Thus, pantothenate kinase regulation involves the competitive binding of CoA to the ATP site, which blocks ATP binding at one site and prevents positive cooperative ATP binding to the second site on the dimer...
  14. ncbi request reprint Acetoacetyl-acyl carrier protein synthase. A target for the antibiotic thiolactomycin
    S Jackowski
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101
    J Biol Chem 264:7624-9. 1989
    ..These data point to acetoacetyl-ACP synthase as a target for thiolactomycin inhibition of bacterial fatty acid biosynthesis...
  15. ncbi request reprint Cloning and characterization of a eukaryotic pantothenate kinase gene (panK) from Aspergillus nidulans
    R B Calder
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 274:2014-20. 1999
    ..Acetyl-CoA inhibition of aPanK was competitive with respect to ATP. Thus, the eukaryotic PanK has a distinct primary structure and unique regulatory properties that clearly distinguish it from its prokaryotic counterpart...
  16. ncbi request reprint Feedback regulation of murine pantothenate kinase 3 by coenzyme A and coenzyme A thioesters
    Yong Mei Zhang
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 280:32594-601. 2005
    ....
  17. ncbi request reprint The structure of the pantothenate kinase.ADP.pantothenate ternary complex reveals the relationship between the binding sites for substrate, allosteric regulator, and antimetabolites
    Robert A Ivey
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 279:35622-9. 2004
    ....
  18. ncbi request reprint Gene structure, expression and identification of a new CTP:phosphocholine cytidylyltransferase beta isoform
    Mohammad Karim
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105 2794, USA
    Biochim Biophys Acta 1633:1-12. 2003
    ..CCTbeta3 is not expressed in embryonic brain tissues, but is a significant transcript in the adult. These data suggest unique roles for the CCT protein isoforms in the differential regulation of PtdCho biosynthesis in specific tissues...
  19. pmc Role of feedback regulation of pantothenate kinase (CoaA) in control of coenzyme A levels in Escherichia coli
    Charles O Rock
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Bacteriol 185:3410-5. 2003
    ..These data illustrate the key role of feedback regulation of pantothenate kinase in the control of intracellular CoA levels...
  20. ncbi request reprint Forty years of bacterial fatty acid synthesis
    Charles O Rock
    The Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, 332 N Lauderdale Street, Memphis, TN 38105, USA
    Biochem Biophys Res Commun 292:1155-66. 2002
  21. ncbi request reprint Acyl carrier protein is a cellular target for the antibacterial action of the pantothenamide class of pantothenate antimetabolites
    Yong Mei Zhang
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 279:50969-75. 2004
    ..Thus, the pantothenamides are pro-antibiotics that inhibit fatty acid synthesis and bacterial growth because of the covalent modification of ACP...
  22. pmc Increased unsaturated fatty acid production associated with a suppressor of the fabA6(Ts) mutation in Escherichia coli
    C O Rock
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101, USA
    J Bacteriol 178:5382-7. 1996
    ..Thus, the suppression of the fabA6(Ts) mutation by sfa was attributed to its ability to increase the biosynthesis of unsaturated fatty acids...
  23. ncbi request reprint Structural basis for the feedback regulation of Escherichia coli pantothenate kinase by coenzyme A
    M Yun
    Departments of Structural Biology and Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 275:28093-9. 2000
    ..The PanK structure provides the framework for a more detailed understanding of the mechanism of catalysis and feedback regulation of PanK...
  24. pmc Cloning, sequence, and expression of the pantothenate permease (panF) gene of Escherichia coli
    S Jackowski
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee
    J Bacteriol 172:3842-8. 1990
    ..The predicted topological profile of pantothenate permease is similar to that of other membrane carriers that catalyze cation-dependent symport...
  25. pmc Probucol therapy overcomes the reproductive defect in CTP: phosphocholine cytidylyltransferase beta2 knockout mice
    Christopher Gunter
    Department of Infectious Diseases, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105 2794, USA
    Biochim Biophys Acta 1771:845-52. 2007
    ..These data indicated that a deficiency in de novo PtdCho synthesis could be complemented by altering the metabolism of serum lipoproteins, an alternative source for cellular phospholipid...
  26. pmc Chemical knockout of pantothenate kinase reveals the metabolic and genetic program responsible for hepatic coenzyme A homeostasis
    Yong Mei Zhang
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Chem Biol 14:291-302. 2007
    ..These results identify the metabolic rearrangements that maintain the CoASH pool which is critical to mitochondrial functions, including gluconeogenesis, fatty acid oxidation, and the tricarboxylic acid and urea cycles...
  27. ncbi request reprint Coenzyme A: back in action
    Roberta Leonardi
    Department of Infectious Diseases, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105 2794, USA
    Prog Lipid Res 44:125-53. 2005
    ..The purpose of this review is to integrate previous knowledge with the most recent findings in the genetics, enzymology and regulation of CoA biosynthesis in bacteria, plants and mammals...
  28. ncbi request reprint Structure and mechanism of CTP:phosphocholine cytidylyltransferase (LicC) from Streptococcus pneumoniae
    Bo Yeon Kwak
    Department of Structural Biology and the Protein Science Division, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 277:4343-50. 2002
    ..These results provide a structural basis for assigning a specific role for magnesium in the catalytic mechanism of pneumococcal LicC...
  29. ncbi request reprint The role of CDP-diacylglycerol synthetase and phosphatidylinositol synthase activity levels in the regulation of cellular phosphatidylinositol content
    A Lykidis
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 272:33402-9. 1997
    ....
  30. ncbi request reprint Sequence and function of the aas gene in Escherichia coli
    S Jackowski
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101
    J Biol Chem 269:2921-8. 1994
    ....
  31. ncbi request reprint Isolation and characterization of Escherichia coli K-12 mutants lacking both 2-acyl-glycerophosphoethanolamine acyltransferase and acyl-acyl carrier protein synthetase activity
    L Hsu
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105
    J Biol Chem 266:13783-8. 1991
    ....
  32. ncbi request reprint Isolation and characterization of the beta-ketoacyl-acyl carrier protein synthase III gene (fabH) from Escherichia coli K-12
    J T Tsay
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105
    J Biol Chem 267:6807-14. 1992
    ..These catalytic properties are consistent with the proposed role of synthase III in the initiation of fatty acid synthesis...
  33. pmc Early embryonic lethality in mice with targeted deletion of the CTP:phosphocholine cytidylyltransferase alpha gene (Pcyt1a)
    Limin Wang
    Department of Infectious Diseases, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105 2794, USA
    Mol Cell Biol 25:3357-63. 2005
    ..These data show that CCTalpha expression is required for early embryonic development, but that a 50% reduction in enzyme activity has little detectable impact on the operation of the CDP-choline metabolic pathway in adult tissues...
  34. pmc CTP:phosphocholine cytidylyltransferase alpha is required for B-cell proliferation and class switch recombination
    Paolo Fagone
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105 3678, USA
    J Biol Chem 284:6847-54. 2009
    ..Thus, the inability of stimulated B-cells to produce enough phosphatidylcholine prevents proliferation and class switch recombination but leads to unfolded protein response activation and a hyper-IgM secretion phenotype...
  35. ncbi request reprint A pantothenate kinase from Staphylococcus aureus refractory to feedback regulation by coenzyme A
    Roberta Leonardi
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 280:3314-22. 2005
    ..aureus, which lacks glutathione and relies on the CoA/CoA disulfide reductase redox system for protection from oxidative damage...
  36. ncbi request reprint PPARalpha controls the intracellular coenzyme A concentration via regulation of PANK1alpha gene expression
    Gayathri Ramaswamy
    Protein Science Division, St Jude Children s Research Hospital, Memphis, TN 38105 2794, USA
    J Lipid Res 45:17-31. 2004
    ....
  37. ncbi request reprint The gene for murine CTP:phosphocholine cytidylyltransferase (Ctpct) is located on mouse chromosome 16
    M S Rutherford
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101
    Genomics 18:698-701. 1993
    ..These data place the Ctpct gene on mouse chromosome 16 between the Smst and Stf-1 genes...
  38. pmc coaA and rts are allelic and located at kilobase 3532 on the Escherichia coli physical map
    W J Song
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101
    J Bacteriol 174:1705-6. 1992
  39. pmc Activation of human mitochondrial pantothenate kinase 2 by palmitoylcarnitine
    Roberta Leonardi
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 104:1494-9. 2007
    ..Our results suggest that PanK2 is located in the mitochondria to sense the levels of palmitoylcarnitine and up-regulate CoA biosynthesis in response to an increased mitochondrial demand for the cofactor to support beta-oxidation...
  40. ncbi request reprint Phospholipid biosynthesis program underlying membrane expansion during B-lymphocyte differentiation
    Paolo Fagone
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
    J Biol Chem 282:7591-605. 2007
    ..This work defines a genetic and biochemical program for membrane phospholipid biogenesis that correlates with an increase in the phospholipid components of the endoplasmic reticulum and Golgi compartments in LPS-stimulated B-cells...
  41. pmc Role of phosphocholine cytidylyltransferase alpha in lung development
    Yong Tian
    Department of Infectious Diseases, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105 2794, USA
    Mol Cell Biol 27:975-82. 2007
    ....
  42. ncbi request reprint Structure-activity relationships and enzyme inhibition of pantothenamide-type pantothenate kinase inhibitors
    Kristopher G Virga
    Department of Pharmaceutical Sciences, University of Tennessee Health Science Center, Memphis, 38163, USA
    Bioorg Med Chem 14:1007-20. 2006
    ..coli enzyme showed greater selectivity. The overall activity data from these analogues suggest a complex and non-enzyme specific SAR for pantothenamide substrate/inhibitors of the different PanK enzymes...
  43. ncbi request reprint The murine pantothenate kinase (Pank1) gene encodes two differentially regulated pantothenate kinase isozymes
    Charles O Rock
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, 332 North Lauderdale, Memphis, TN 38105 2794, USA
    Gene 291:35-43. 2002
    ..Differential expression of the PanK1 alpha and PanK1 beta transcripts would alter the amount of CoA produced in cells as a function of the ratio of free CoA to acetyl-CoA, a reflection of the metabolic status of the tissue...
  44. pmc A missense mutation in the fabB (beta-ketoacyl-acyl carrier protein synthase I) gene confers tiolactomycin resistance to Escherichia coli
    Suzanne Jackowski
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Antimicrob Agents Chemother 46:1246-52. 2002
    ..These data illustrate that missense mutations that introduce valine at position 390 confer TLM resistance while maintaining the vital catalytic properties of FabB...
  45. ncbi request reprint Cloning of a novel phosphoprotein regulated by colony-stimulating factor 1 shares a domain with the Drosophila disabled gene product
    X X Xu
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101, USA
    J Biol Chem 270:14184-91. 1995
    ..These data identify a novel protein that is phosphorylated in response to mitogenic growth factor stimulation...
  46. pmc Localization and regulation of mouse pantothenate kinase 2
    Roberta Leonardi
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105, United States
    FEBS Lett 581:4639-44. 2007
    ..Differences in expression and subcellular localization should be considered in developing a mouse model for human PanK2 deficiency...
  47. pmc Pantothenate kinase 1 is required to support the metabolic transition from the fed to the fasted state
    Roberta Leonardi
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee, United States of America
    PLoS ONE 5:e11107. 2010
    ..The findings also suggest that PanK1 may be a suitable target for therapeutic intervention in metabolic disorders that feature hyperglycemia and hypertriglyceridemia...
  48. pmc Elimination of the CDP-ethanolamine pathway disrupts hepatic lipid homeostasis
    Roberta Leonardi
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105 3678, USA
    J Biol Chem 284:27077-89. 2009
    ..The absence of the ECT pathway for diacylglycerol utilization at the endoplasmic reticulum triggers increased fatty acid synthesis to support the formation of triacylglycerols leading to liver steatosis...
  49. ncbi request reprint Placental thrombosis and spontaneous fetal death in mice deficient in ethanolamine kinase 2
    Yong Tian
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
    J Biol Chem 281:28438-49. 2006
    ..These results illustrate a non-redundant role for EtnK2 expression in regulating placental hemostasis...
  50. pmc Bacterial inhibition of phosphatidylcholine synthesis triggers apoptosis in the brain
    Janine Zweigner
    Department of Infectious Diseases, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105, USA
    J Exp Med 200:99-106. 2004
    ..We conclude that bacterial inhibition of PtdCho biosynthesis activates an apoptotic cascade that is a causative event in pathogenesis and amenable to therapeutic intervention...
  51. pmc Structure of the type III pantothenate kinase from Bacillus anthracis at 2.0 A resolution: implications for coenzyme A-dependent redox biology
    Nathan I Nicely
    Center for Structural Biology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    Biochemistry 46:3234-45. 2007
    ..Our analyses also suggest that the type III PanK in the spore-forming B. anthracis plays an essential role in the novel thiol/disulfide redox biology of this category A biodefense pathogen...
  52. ncbi request reprint Crystal structures of human pantothenate kinases. Insights into allosteric regulation and mutations linked to a neurodegeneration disorder
    Bum Soo Hong
    Structural Genomics Consortium and Department of Pharmacology, University of Toronto, Toronto, Ontario M5G 1L5, Canada
    J Biol Chem 282:27984-93. 2007
    ....
  53. ncbi request reprint Coordinate regulation of phospholipid biosynthesis and secretory pathway gene expression in XBP-1(S)-induced endoplasmic reticulum biogenesis
    Rungtawan Sriburi
    Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois 60153, USA
    J Biol Chem 282:7024-34. 2007
    ..We propose that XBP-1(S) regulates ER abundance by coordinately increasing the supply of membrane phospholipids and ER proteins, the key ingredients for ER biogenesis...
  54. pmc XBP1: a link between the unfolded protein response, lipid biosynthesis, and biogenesis of the endoplasmic reticulum
    Rungtawan Sriburi
    Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA
    J Cell Biol 167:35-41. 2004
    ..These data suggest that XBP1(S) links the mammalian UPR to phospholipid biosynthesis and ER biogenesis...
  55. ncbi request reprint Inhibition of CTP:phosphocholine cytidylyltransferase by C(2)-ceramide and its relationship to apoptosis
    Belen Ramos
    Departamento de Fisiologia, Universidad de Extremadura, Caceres, Spain
    Mol Pharmacol 62:1068-75. 2002
    ..Taken together, our results suggest CCT as a primary target for C(2)-ceramide inhibition that accounts for its cytotoxic effects...

Research Grants25

  1. Regulation of Coenzyme A Levels by Panothenate Kinase
    Suzanne Jackowski; Fiscal Year: 2010
    ..The research requires expertise in the areas of CoA biosynthesis, lipid metabolism and protein biochemistry which are the clear strengths of the investigative team assembled to pursue this problem. ..
  2. Regulation of Coenzyme A Levels by Pantothenate Kinase
    Suzanne Jackowski; Fiscal Year: 2005
    ..This research will contribute to the understanding of global metabolic control and human disease by defining the importance of CoA regulation by specific PanK isozymes. ..
  3. REGULATION OF PHOSPHOLIPID SYNTHESIS
    Suzanne Jackowski; Fiscal Year: 2006
    ..The functions of the CCT regulatory domains characterized in vitro will also be evaluated for cellular localization and membrane binding in living cells. ..
  4. Regulation of Coenzyme A Levels by Panothenate Kinase
    Suzanne Jackowski; Fiscal Year: 2007
    ..The research requires expertise in the areas of CoA biosynthesis, lipid metabolism and protein biochemistry which are the clear strengths of the investigative team assembled to pursue this problem. ..
  5. Regulation of Coenzyme A Levels by Panothenate Kinase
    Suzanne Jackowski; Fiscal Year: 2009
    ..The research requires expertise in the areas of CoA biosynthesis, lipid metabolism and protein biochemistry which are the clear strengths of the investigative team assembled to pursue this problem. ..
  6. REGULATION OF MEMBRANE PHOSPHOLIPID SYNTHESIS
    Suzanne Jackowski; Fiscal Year: 2002
    ..This research will advance our understanding of the factors that influence cell membrane synthesis and trafficking and then mechanisms that control phospholipid homeostasis in growing cells. ..
  7. REGULATION OF MEMBRANE BIOSYNTHESIS BY CSF-1
    Suzanne Jackowski; Fiscal Year: 1993
    ....