T Izard

Summary

Affiliation: St. Jude Children's Research Hospital
Country: USA

Publications

  1. pmc The crystal structures of chloramphenicol phosphotransferase reveal a novel inactivation mechanism
    T Izard
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
    EMBO J 19:2690-700. 2000
  2. ncbi request reprint Structural basis for amplifying vinculin activation by talin
    Tina Izard
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 279:27667-78. 2004
  3. ncbi request reprint Vinculin activation by talin through helical bundle conversion
    Tina Izard
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Nature 427:171-5. 2004
  4. pmc A novel adenylate binding site confers phosphopantetheine adenylyltransferase interactions with coenzyme A
    Tina Izard
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
    J Bacteriol 185:4074-80. 2003
  5. pmc Shigella applies molecular mimicry to subvert vinculin and invade host cells
    Tina Izard
    Cell Adhesion Laboratory, Department of Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    J Cell Biol 175:465-75. 2006
  6. ncbi request reprint The crystal structures of phosphopantetheine adenylyltransferase with bound substrates reveal the enzyme's catalytic mechanism
    Tina Izard
    Department of Structural Biology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105 2794, USA
    J Mol Biol 315:487-95. 2002
  7. pmc Structural basis for chloramphenicol tolerance in Streptomyces venezuelae by chloramphenicol phosphotransferase activity
    T Izard
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
    Protein Sci 10:1508-13. 2001
  8. pmc Crystal structures of the metal-dependent 2-dehydro-3-deoxy-galactarate aldolase suggest a novel reaction mechanism
    T Izard
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
    EMBO J 19:3849-56. 2000
  9. pmc Raver1 interactions with vinculin and RNA suggest a feed-forward pathway in directing mRNA to focal adhesions
    Jun Hyuck Lee
    Cell Adhesion Laboratory, Department of Cancer Biology, The Scripps Research Institute, Jupiter, FL 33458, USA
    Structure 17:833-42. 2009
  10. pmc The crystal structure of a novel bacterial adenylyltransferase reveals half of sites reactivity
    T Izard
    Department of Biochemistry, University of Leicester, Leicester LE1
    EMBO J 18:2021-30. 1999

Collaborators

Detail Information

Publications27

  1. pmc The crystal structures of chloramphenicol phosphotransferase reveal a novel inactivation mechanism
    T Izard
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
    EMBO J 19:2690-700. 2000
    ..Comparison between liganded and substrate-free CPT structures highlights side chain movements of the active site's Arg136 guanidinium group of >9 A upon substrate binding...
  2. ncbi request reprint Structural basis for amplifying vinculin activation by talin
    Tina Izard
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 279:27667-78. 2004
    ..These findings support a model in which talin binds to and activates multiple vinculin molecules to provoke rapid reorganization of the actin cytoskeleton...
  3. ncbi request reprint Vinculin activation by talin through helical bundle conversion
    Tina Izard
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Nature 427:171-5. 2004
    ..Notably, talin's effects on Vh structure establish helical bundle conversion as a signalling mechanism by which proteins direct cellular responses...
  4. pmc A novel adenylate binding site confers phosphopantetheine adenylyltransferase interactions with coenzyme A
    Tina Izard
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
    J Bacteriol 185:4074-80. 2003
    ....
  5. pmc Shigella applies molecular mimicry to subvert vinculin and invade host cells
    Tina Izard
    Cell Adhesion Laboratory, Department of Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    J Cell Biol 175:465-75. 2006
    ..flexneri utilizes a remarkable level of molecular mimicry of the talin-vinculin interaction to activate vinculin. Mimicry of vinculin's interactions may therefore be a general mechanism applied by pathogens to infect the host cell...
  6. ncbi request reprint The crystal structures of phosphopantetheine adenylyltransferase with bound substrates reveal the enzyme's catalytic mechanism
    Tina Izard
    Department of Structural Biology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105 2794, USA
    J Mol Biol 315:487-95. 2002
    ..Within the PPAT hexamer, one trimer is in its substrate-free state, whereas the other is in a substrate-bound state...
  7. pmc Structural basis for chloramphenicol tolerance in Streptomyces venezuelae by chloramphenicol phosphotransferase activity
    T Izard
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
    Protein Sci 10:1508-13. 2001
    ..9 A resolution. Surprisingly, p-NH(2)-Cm binds CPT in a novel fashion. Additionally, neither 2-N-Ac-Cm nor p-NH(2)-Cm binds to the secondary product binding site...
  8. pmc Crystal structures of the metal-dependent 2-dehydro-3-deoxy-galactarate aldolase suggest a novel reaction mechanism
    T Izard
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
    EMBO J 19:3849-56. 2000
    ..Analysis of the DDG aldolase crystal structures suggests a novel aldolase mechanism in which a phosphate anion accepts the proton from the methyl group of pyruvate...
  9. pmc Raver1 interactions with vinculin and RNA suggest a feed-forward pathway in directing mRNA to focal adhesions
    Jun Hyuck Lee
    Cell Adhesion Laboratory, Department of Cancer Biology, The Scripps Research Institute, Jupiter, FL 33458, USA
    Structure 17:833-42. 2009
    ..These findings suggest a feed-forward model where vinculin activation at focal adhesions provides a scaffold for recruitment of raver1 and its mRNA cargo to facilitate the production of components of adhesion complexes...
  10. pmc The crystal structure of a novel bacterial adenylyltransferase reveals half of sites reactivity
    T Izard
    Department of Biochemistry, University of Leicester, Leicester LE1
    EMBO J 18:2021-30. 1999
    ..The homologous active site attachment of ATP and the structural distribution of predicted sequence-binding motifs in PPAT classify the enzyme as belonging to the nucleotidyltransferase superfamily...
  11. ncbi request reprint The vinculin binding sites of talin and alpha-actinin are sufficient to activate vinculin
    Philippe R J Bois
    Department of Biochemistry, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105, USA
    J Biol Chem 281:7228-36. 2006
    ....
  12. ncbi request reprint Rhombohedral crystals of 2-dehydro-3-deoxygalactarate aldolase from Escherichia coli
    N C Blackwell
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, England
    Acta Crystallogr D Biol Crystallogr 55:1368-9. 1999
    ..The crystals diffract to beyond 1.8 A resolution on a Cu Kalpha rotating-anode generator. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 1.34 A3 Da-1...
  13. ncbi request reprint Rhombohedral crystals of the human vinculin head domain in complex with a vinculin-binding site of talin
    Christina L Rush
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Acta Crystallogr D Biol Crystallogr 60:945-7. 2004
    ..7 A, alpha = 105.5 degrees and diffract to 2.4 A on a third-generation synchrotron source. The packing density for one heterodimer in the asymmetric unit is 3.04 A(3) Da(-1), with a solvent content of 0.59...
  14. ncbi request reprint Cubic crystals of phosphopantetheine adenylyltransferase from Escherichia coli
    T Izard
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, England
    Acta Crystallogr D Biol Crystallogr 55:1226-8. 1999
    ..5 A. The crystals diffract to better than 1.8 A resolution on a Cu Kalpha rotating-anode generator. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 2.9 A3 Da-1...
  15. pmc Structural dynamics of alpha-actinin-vinculin interactions
    Philippe R J Bois
    Department of Hematology Oncology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, Tennessee 38105, USA
    Mol Cell Biol 25:6112-22. 2005
    ..The data suggest a model where vinculin's Vh1 domain acts as a molecular switch that undergoes distinct structural changes provoked by talin and alpha-actinin binding in focal adhesions versus adherens junctions, respectively...
  16. pmc Intermolecular versus intramolecular interactions of the vinculin binding site 33 of talin
    S D Yogesha
    Cell Adhesion Laboratory, Department of Cancer Biology, The Scripps Research Institute, Jupiter, FL 33458, USA
    Protein Sci 20:1471-6. 2011
    ....
  17. ncbi request reprint Crystal structure of human vinculin
    Robert A Borgon
    Department of Hematology Oncology, St Jude Children s Research Hospital, 332 N Lauderdale Street, Memphis, TN 38105, USA
    Structure 12:1189-97. 2004
    ....
  18. pmc Substrate-induced asymmetry and channel closure revealed by the apoenzyme structure of Mycobacterium tuberculosis phosphopantetheine adenylyltransferase
    Van K Morris
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis TN 38105, USA
    Protein Sci 13:2547-52. 2004
    ..In its Apo form, PPAT is a symmetric hexamer with an open solvent channel. However, ligand binding provokes asymmetry and alters the structure of the solvent channel, so that ligand binding becomes restricted to one trimer...
  19. ncbi request reprint Rhombohedral crystals of Mycobacterium tuberculosis phosphopantetheine adenylyltransferase
    Kathrin L Brown
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Acta Crystallogr D Biol Crystallogr 60:195-6. 2004
    ..81 degrees. The crystals diffract to better than 2 A resolution on a Cu Kalpha rotating-anode generator. The packing density for one polypeptide chain in the asymmetric unit is 2.89 A(3) Da(-1), with a solvent content of 0.57...
  20. pmc Dramatic improvement of crystal quality for low-temperature-grown rabbit muscle aldolase
    HaJeung Park
    Cell Adhesion Laboratory, Department of Cancer Biology, The Scripps Research Institute, Jupiter, FL 33458, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:595-600. 2010
    ..The thermodynamically unfavorable immobilization of high-entropy side chains used in lattice formation was compensated by an entropic increase in the bulk-solvent content owing to the greater solvent content of the crystal lattice...
  21. ncbi request reprint Induced fit movements and metal cofactor selectivity of class II aldolases: structure of Thermus aquaticus fructose-1,6-bisphosphate aldolase
    Tina Izard
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38111, USA
    J Biol Chem 279:11825-33. 2004
    ..Comparison with the E. coli structure also indicates a structural basis for thermostability by Taq FBP aldolase...
  22. ncbi request reprint Crystallization of HLA-DR4 fused to an immunodominant collagen II peptide implicated in rheumatoid arthritis
    Jason C Hurlbert
    Department of Structural Biology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105, USA
    Acta Crystallogr D Biol Crystallogr 58:1749-51. 2002
    ..Crystallization time, crystal size, and reproducibility were greatly improved by macroseeding into microdialysis buttons...
  23. pmc Mapping the interaction surface of linker histone H1(0) with the nucleosome of native chromatin in vivo
    David T Brown
    Department of Biochemistry, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA
    Nat Struct Mol Biol 13:250-5. 2006
    ..Multiple residues bind cooperatively to form a highly specific chromatosome structure that provides a mechanism by which individual domains of linker histones interact to facilitate chromatin condensation...
  24. pmc Nucleosome interaction surface of linker histone H1c is distinct from that of H1(0)
    Eric M George
    Department of Biochemistry, University of Mississippi Medical Center, Jackson, MI 39216 4505, USA
    J Biol Chem 285:20891-6. 2010
    ....
  25. ncbi request reprint A hydrophobic pocket in the active site of glycolytic aldolase mediates interactions with Wiskott-Aldrich syndrome protein
    Miguel St-Jean
    Department of Biochemistry, University of Montreal, Montreal, Quebec, Canada
    J Biol Chem 282:14309-15. 2007
    ..The data support a model whereby aldolase exists in distinct forms that regulate glycolysis or actin dynamics...
  26. pmc Vinculin binding in its closed conformation by a helix addition mechanism
    Guy Tran Van Nhieu
    Unite de Pathogenie Microbienne Moleculaire, Institut Pasteur, INSERM U786, Paris, France
    EMBO J 26:4588-96. 2007
    ....
  27. ncbi request reprint Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain
    Nalini Ramarao
    Unite de Pathogenie Microbienne Moleculaire, INSERM U786, Institut Pasteur, 75724 Paris Cedex 15, France
    FEBS Lett 581:853-7. 2007
    ..These results suggest that IpaA regulates actin polymerisation/depolymerisation at sites of Shigella invasion by modulating the barbed end capping activity of vinculin...