Research Topics
Genomes and Genes | T IzardSummaryAffiliation: St. Jude Children's Research Hospital Country: USA Publications
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Publications
The crystal structures of chloramphenicol phosphotransferase reveal a novel inactivation mechanismT Izard
Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
EMBO J 19:2690-700. 2000..Comparison between liganded and substrate-free CPT structures highlights side chain movements of the active site's Arg136 guanidinium group of >9 A upon substrate binding...
Structural basis for amplifying vinculin activation by talinTina Izard
Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
J Biol Chem 279:27667-78. 2004..These findings support a model in which talin binds to and activates multiple vinculin molecules to provoke rapid reorganization of the actin cytoskeleton...- Vinculin activation by talin through helical bundle conversionTina Izard
Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
Nature 427:171-5. 2004..Notably, talin's effects on Vh structure establish helical bundle conversion as a signalling mechanism by which proteins direct cellular responses... - A novel adenylate binding site confers phosphopantetheine adenylyltransferase interactions with coenzyme ATina Izard
Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
J Bacteriol 185:4074-80. 2003.... - Shigella applies molecular mimicry to subvert vinculin and invade host cellsTina Izard
Cell Adhesion Laboratory, Department of Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
J Cell Biol 175:465-75. 2006..flexneri utilizes a remarkable level of molecular mimicry of the talin-vinculin interaction to activate vinculin. Mimicry of vinculin's interactions may therefore be a general mechanism applied by pathogens to infect the host cell... - The crystal structures of phosphopantetheine adenylyltransferase with bound substrates reveal the enzyme's catalytic mechanismTina Izard
Department of Structural Biology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105 2794, USA
J Mol Biol 315:487-95. 2002..Within the PPAT hexamer, one trimer is in its substrate-free state, whereas the other is in a substrate-bound state... - Structural basis for chloramphenicol tolerance in Streptomyces venezuelae by chloramphenicol phosphotransferase activityT Izard
Department of Structural Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
Protein Sci 10:1508-13. 2001..9 A resolution. Surprisingly, p-NH(2)-Cm binds CPT in a novel fashion. Additionally, neither 2-N-Ac-Cm nor p-NH(2)-Cm binds to the secondary product binding site... - Crystal structures of the metal-dependent 2-dehydro-3-deoxy-galactarate aldolase suggest a novel reaction mechanismT Izard
Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
EMBO J 19:3849-56. 2000..Analysis of the DDG aldolase crystal structures suggests a novel aldolase mechanism in which a phosphate anion accepts the proton from the methyl group of pyruvate... - Raver1 interactions with vinculin and RNA suggest a feed-forward pathway in directing mRNA to focal adhesionsJun Hyuck Lee
Cell Adhesion Laboratory, Department of Cancer Biology, The Scripps Research Institute, Jupiter, FL 33458, USA
Structure 17:833-42. 2009..These findings suggest a feed-forward model where vinculin activation at focal adhesions provides a scaffold for recruitment of raver1 and its mRNA cargo to facilitate the production of components of adhesion complexes... - The crystal structure of a novel bacterial adenylyltransferase reveals half of sites reactivityT Izard
Department of Biochemistry, University of Leicester, Leicester LE1
EMBO J 18:2021-30. 1999..The homologous active site attachment of ATP and the structural distribution of predicted sequence-binding motifs in PPAT classify the enzyme as belonging to the nucleotidyltransferase superfamily... - The vinculin binding sites of talin and alpha-actinin are sufficient to activate vinculinPhilippe R J Bois
Department of Biochemistry, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105, USA
J Biol Chem 281:7228-36. 2006.... - Rhombohedral crystals of 2-dehydro-3-deoxygalactarate aldolase from Escherichia coliN C Blackwell
Department of Biochemistry, University of Leicester, Leicester LE1 7RH, England
Acta Crystallogr D Biol Crystallogr 55:1368-9. 1999..The crystals diffract to beyond 1.8 A resolution on a Cu Kalpha rotating-anode generator. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 1.34 A3 Da-1... - Rhombohedral crystals of the human vinculin head domain in complex with a vinculin-binding site of talinChristina L Rush
Department of Hematology-Oncology, St Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
Acta Crystallogr D Biol Crystallogr 60:945-7. 2004..7 A, alpha = 105.5 degrees and diffract to 2.4 A on a third-generation synchrotron source. The packing density for one heterodimer in the asymmetric unit is 3.04 A(3) Da(-1), with a solvent content of 0.59... - Cubic crystals of phosphopantetheine adenylyltransferase from Escherichia coliT Izard
Department of Biochemistry, University of Leicester, Leicester LE1 7RH, England
Acta Crystallogr D Biol Crystallogr 55:1226-8. 1999..5 A. The crystals diffract to better than 1.8 A resolution on a Cu Kalpha rotating-anode generator. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 2.9 A3 Da-1... - Structural dynamics of alpha-actinin-vinculin interactionsPhilippe R J Bois
Department of Hematology Oncology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, Tennessee 38105, USA
Mol Cell Biol 25:6112-22. 2005..The data suggest a model where vinculin's Vh1 domain acts as a molecular switch that undergoes distinct structural changes provoked by talin and alpha-actinin binding in focal adhesions versus adherens junctions, respectively... - Intermolecular versus intramolecular interactions of the vinculin binding site 33 of talinS D Yogesha
Cell Adhesion Laboratory, Department of Cancer Biology, The Scripps Research Institute, Jupiter, FL 33458, USA
Protein Sci 20:1471-6. 2011.... - Crystal structure of human vinculinRobert A Borgon
Department of Hematology-Oncology, St. Jude Children's Research Hospital, 332 N. Lauderdale Street, Memphis, TN 38105, USA
Structure 12:1189-97. 2004.... - Substrate-induced asymmetry and channel closure revealed by the apoenzyme structure of Mycobacterium tuberculosis phosphopantetheine adenylyltransferaseVan K Morris
Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis TN 38105, USA
Protein Sci 13:2547-52. 2004..In its Apo form, PPAT is a symmetric hexamer with an open solvent channel. However, ligand binding provokes asymmetry and alters the structure of the solvent channel, so that ligand binding becomes restricted to one trimer... - Rhombohedral crystals of Mycobacterium tuberculosis phosphopantetheine adenylyltransferaseKathrin L Brown
Department of Hematology-Oncology, St Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
Acta Crystallogr D Biol Crystallogr 60:195-6. 2004..81 degrees. The crystals diffract to better than 2 A resolution on a Cu Kalpha rotating-anode generator. The packing density for one polypeptide chain in the asymmetric unit is 2.89 A(3) Da(-1), with a solvent content of 0.57... - Dramatic improvement of crystal quality for low-temperature-grown rabbit muscle aldolaseHaJeung Park
Cell Adhesion Laboratory, Department of Cancer Biology, The Scripps Research Institute, Jupiter, FL 33458, USA
Acta Crystallogr Sect F Struct Biol Cryst Commun 66:595-600. 2010..The thermodynamically unfavorable immobilization of high-entropy side chains used in lattice formation was compensated by an entropic increase in the bulk-solvent content owing to the greater solvent content of the crystal lattice... - Induced fit movements and metal cofactor selectivity of class II aldolases: structure of Thermus aquaticus fructose-1,6-bisphosphate aldolaseTina Izard
Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38111, USA
J Biol Chem 279:11825-33. 2004..Comparison with the E. coli structure also indicates a structural basis for thermostability by Taq FBP aldolase... - Crystallization of HLA-DR4 fused to an immunodominant collagen II peptide implicated in rheumatoid arthritisJason C Hurlbert
Department of Structural Biology, St Jude Children's Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105, USA
Acta Crystallogr D Biol Crystallogr 58:1749-51. 2002..Crystallization time, crystal size, and reproducibility were greatly improved by macroseeding into microdialysis buttons... - Mapping the interaction surface of linker histone H1(0) with the nucleosome of native chromatin in vivoDavid T Brown
Department of Biochemistry, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA
Nat Struct Mol Biol 13:250-5. 2006..Multiple residues bind cooperatively to form a highly specific chromatosome structure that provides a mechanism by which individual domains of linker histones interact to facilitate chromatin condensation... - Nucleosome interaction surface of linker histone H1c is distinct from that of H1(0)Eric M George
Department of Biochemistry, University of Mississippi Medical Center, Jackson, MI 39216 4505, USA
J Biol Chem 285:20891-6. 2010.... - A hydrophobic pocket in the active site of glycolytic aldolase mediates interactions with Wiskott-Aldrich syndrome proteinMiguel St-Jean
Department of Biochemistry, University of Montreal, Montreal, Quebec, Canada
J Biol Chem 282:14309-15. 2007..The data support a model whereby aldolase exists in distinct forms that regulate glycolysis or actin dynamics... - Vinculin binding in its closed conformation by a helix addition mechanismGuy Tran Van Nhieu
Unite de Pathogenie Microbienne Moleculaire, Institut Pasteur, INSERM U786, Paris, France
EMBO J 26:4588-96. 2007.... - Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domainNalini Ramarao
Unite de Pathogenie Microbienne Moleculaire, INSERM U786, Institut Pasteur, 75724 Paris Cedex 15, France
FEBS Lett 581:853-7. 2007..These results suggest that IpaA regulates actin polymerisation/depolymerisation at sites of Shigella invasion by modulating the barbed end capping activity of vinculin...