R J Heath

Summary

Affiliation: St. Jude Children's Research Hospital
Country: USA

Publications

  1. ncbi Fatty acid biosynthesis as a target for novel antibacterials
    Richard J Heath
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Curr Opin Investig Drugs 5:146-53. 2004
  2. ncbi Inhibitors of fatty acid synthesis as antimicrobial chemotherapeutics
    R J Heath
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis TN 38105, USA
    Appl Microbiol Biotechnol 58:695-703. 2002
  3. ncbi Lipid biosynthesis as a target for antibacterial agents
    R J Heath
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, Tennessee 38105, Memphis, USA
    Prog Lipid Res 40:467-97. 2001
  4. ncbi The FadR.DNA complex. Transcriptional control of fatty acid metabolism in Escherichia coli
    Y Xu
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 276:17373-9. 2001
  5. ncbi Regulation of fatty acid elongation and initiation by acyl-acyl carrier protein in Escherichia coli
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101, USA
    J Biol Chem 271:1833-6. 1996
  6. ncbi Roles of the FabA and FabZ beta-hydroxyacyl-acyl carrier protein dehydratases in Escherichia coli fatty acid biosynthesis
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101, USA
    J Biol Chem 271:27795-801. 1996
  7. ncbi The licC gene of Streptococcus pneumoniae encodes a CTP:phosphocholine cytidylyltransferase
    C O Rock
    Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
    J Bacteriol 183:4927-31. 2001
  8. ncbi Identification and analysis of the acyl carrier protein (ACP) docking site on beta-ketoacyl-ACP synthase III
    Y M Zhang
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 276:8231-8. 2001
  9. ncbi The 1.8 A crystal structure and active-site architecture of beta-ketoacyl-acyl carrier protein synthase III (FabH) from escherichia coli
    C Davies
    Department of Structural Biology, University of Tennessee, School of Biological Sciences, University of Sussex, Memphis, Falmer, 38163, BN1 9QG, USA, UK
    Structure 8:185-95. 2000
  10. ncbi Inhibition of the Staphylococcus aureus NADPH-dependent enoyl-acyl carrier protein reductase by triclosan and hexachlorophene
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 275:4654-9. 2000

Collaborators

Detail Information

Publications18

  1. ncbi Fatty acid biosynthesis as a target for novel antibacterials
    Richard J Heath
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Curr Opin Investig Drugs 5:146-53. 2004
    ..Other experimental compounds such as thiolactomycin and cerulenin effectively inhibit other steps. These known pathway inhibitors are reviewed and the areas for potential future developments are explored...
  2. ncbi Inhibitors of fatty acid synthesis as antimicrobial chemotherapeutics
    R J Heath
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis TN 38105, USA
    Appl Microbiol Biotechnol 58:695-703. 2002
    ..The mechanisms of action of these compounds, as well as the potential development of new drugs targeted against this pathway, are discussed...
  3. ncbi Lipid biosynthesis as a target for antibacterial agents
    R J Heath
    Protein Science Division, Department of Infectious Diseases, St Jude Children s Research Hospital, Tennessee 38105, Memphis, USA
    Prog Lipid Res 40:467-97. 2001
    ..This review will summarize the data generated on these inhibitors to date, and examine where additional efforts will be required to develop new chemotherapeutics to help combat microbial infections...
  4. ncbi The FadR.DNA complex. Transcriptional control of fatty acid metabolism in Escherichia coli
    Y Xu
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 276:17373-9. 2001
    ..We suggest that the binding of acyl-CoA disrupts a buried network of charged and polar residues in the C-terminal domain, and the resulting conformational change is transmitted to the N-terminal domain via a domain-spanning alpha-helix...
  5. ncbi Regulation of fatty acid elongation and initiation by acyl-acyl carrier protein in Escherichia coli
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101, USA
    J Biol Chem 271:1833-6. 1996
    ....
  6. ncbi Roles of the FabA and FabZ beta-hydroxyacyl-acyl carrier protein dehydratases in Escherichia coli fatty acid biosynthesis
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101, USA
    J Biol Chem 271:27795-801. 1996
    ....
  7. ncbi The licC gene of Streptococcus pneumoniae encodes a CTP:phosphocholine cytidylyltransferase
    C O Rock
    Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
    J Bacteriol 183:4927-31. 2001
    ..Phosphoethanolamine is a poor substrate. The LicC protein plays a role in the biosynthesis of the phosphocholine-derivatized cell wall constituents that are critical for cell separation and pathogenesis...
  8. ncbi Identification and analysis of the acyl carrier protein (ACP) docking site on beta-ketoacyl-ACP synthase III
    Y M Zhang
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 276:8231-8. 2001
    ....
  9. ncbi The 1.8 A crystal structure and active-site architecture of beta-ketoacyl-acyl carrier protein synthase III (FabH) from escherichia coli
    C Davies
    Department of Structural Biology, University of Tennessee, School of Biological Sciences, University of Sussex, Memphis, Falmer, 38163, BN1 9QG, USA, UK
    Structure 8:185-95. 2000
    ..Although the structures of two related condensing enzymes are known, the roles of the active-site residues have not been experimentally tested...
  10. ncbi Inhibition of the Staphylococcus aureus NADPH-dependent enoyl-acyl carrier protein reductase by triclosan and hexachlorophene
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 275:4654-9. 2000
    ..Thus, the formation of this ternary complex is a key determinant of the antibacterial activity of FabI inhibitors...
  11. ncbi A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101, USA
    J Bacteriol 181:1944-6. 1999
    ..The PlsB26 protein had a significantly reduced glycerol-3-phosphate acyltransferase specific activity coupled with an elevated Km for glycerol-3-phosphate...
  12. ncbi Inhibition of beta-ketoacyl-acyl carrier protein synthases by thiolactomycin and cerulenin. Structure and mechanism
    A C Price
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 276:6551-9. 2001
    ..These data provide a structural framework for understanding antibiotic sensitivity within this group of enzymes...
  13. ncbi Mechanism of triclosan inhibition of bacterial fatty acid synthesis
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 274:11110-4. 1999
    ....
  14. ncbi beta-ketoacyl-acyl carrier protein synthase III (FabH) is a determining factor in branched-chain fatty acid biosynthesis
    K H Choi
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Bacteriol 182:365-70. 2000
    ..Expression of either bFabH1 or bFabH2 in E. coli resulted in the appearance of a branched-chain 17-carbon fatty acid. Thus, the substrate specificity of FabH is an important determinant of branched-chain fatty acid production...
  15. ncbi A conserved histidine is essential for glycerolipid acyltransferase catalysis
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Bacteriol 180:1425-30. 1998
    ..These data are consistent with a mechanism for glycerolipid acyltransferase catalysis where the invariant histidine functions as a general base to deprotonate the hydroxyl moiety of the acyl acceptor...
  16. ncbi Inhibition of beta-ketoacyl-acyl carrier protein synthase III (FabH) by acyl-acyl carrier protein in Escherichia coli
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101, USA
    J Biol Chem 271:10996-1000. 1996
    ..These data support the concept that the inhibition of chain initiation at the beta-ketoacyl-ACP synthase III step contributes to the attenuation of fatty acid biosynthesis by acyl-ACP...
  17. ncbi Enoyl-acyl carrier protein reductase (fabI) plays a determinant role in completing cycles of fatty acid elongation in Escherichia coli
    R J Heath
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38101, USA
    J Biol Chem 270:26538-42. 1995
    ..We conclude that FabI is the only enoyl-ACP reductase involved in fatty acid synthesis in E. coli and that the activity of this enzyme plays a determinant role in completing cycles of fatty acid biosynthesis...
  18. ncbi The Claisen condensation in biology
    Richard J Heath
    Department of Infectious Diseases, St Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
    Nat Prod Rep 19:581-96. 2002
    ..This review covers the recent structural and mechanistic data to see if a unifying hypothesis for the reaction mechanism catalyzed by this important family of enzymes can be established...