R T Hayden

Summary

Affiliation: St. Jude Children's Research Hospital
Country: USA

Publications

  1. pmc Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus
    R T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    J Clin Microbiol 51:540-6. 2013
  2. doi request reprint Comparison of two broadly multiplexed PCR systems for viral detection in clinical respiratory tract specimens from immunocompromised children
    Randall T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, TN 38105, United States
    J Clin Virol 53:308-13. 2012
  3. pmc Factors contributing to variability of quantitative viral PCR results in proficiency testing samples: a multivariate analysis
    R T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    J Clin Microbiol 50:337-45. 2012
  4. doi request reprint A survey-based assessment of United States clinical laboratory response to the 2009 H1N1 influenza outbreak
    Randall T Hayden
    Department of Pathology, St JudeChildren s Research Hospital, Memphis, TN 38105 2794, USA
    Arch Pathol Lab Med 134:1671-8. 2010
  5. ncbi request reprint Characterization of culture-derived spiral bacteria by 16S ribosomal RNA gene sequence analysis
    R T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    Diagn Microbiol Infect Dis 39:55-9. 2001
  6. ncbi request reprint In situ hybridization for the differentiation of Aspergillus, Fusarium, and Pseudallescheria species in tissue section
    R T Hayden
    Children s Research Hospital, Memphis, TN, USA
    Diagn Mol Pathol 12:21-6. 2003
  7. ncbi request reprint In situ hybridization for the identification of filamentous fungi in tissue section
    R T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee 38103, USA
    Diagn Mol Pathol 11:119-26. 2002
  8. pmc Multicenter comparison of different real-time PCR assays for quantitative detection of Epstein-Barr virus
    R T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    J Clin Microbiol 46:157-63. 2008
  9. ncbi request reprint Quantitative real-time PCR detection of adenovirus in clinical blood specimens: a comparison of plasma, whole blood and peripheral blood mononuclear cells
    J Perlman
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN, United States
    J Clin Virol 40:295-300. 2007
  10. pmc Multicenter evaluation of a commercial cytomegalovirus quantitative standard: effects of commutability on interlaboratory concordance
    R T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    J Clin Microbiol 51:3811-7. 2013

Collaborators

Detail Information

Publications39

  1. pmc Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus
    R T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    J Clin Microbiol 51:540-6. 2013
    ..Digital PCR may provide an opportunity to reduce the quantitative variability currently seen using real-time PCR, but methods need to be further optimized to match the sensitivity of real-time PCR...
  2. doi request reprint Comparison of two broadly multiplexed PCR systems for viral detection in clinical respiratory tract specimens from immunocompromised children
    Randall T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, TN 38105, United States
    J Clin Virol 53:308-13. 2012
    ..The detection of viral respiratory tract infections has evolved greatly with the development of PCR based commercial systems capable of simultaneously detecting a wide variety of pathogens...
  3. pmc Factors contributing to variability of quantitative viral PCR results in proficiency testing samples: a multivariate analysis
    R T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    J Clin Microbiol 50:337-45. 2012
    ..The reduction of such variability will require a multifaceted approach to improve the accuracy, reliability, and clinical utility of these important tests...
  4. doi request reprint A survey-based assessment of United States clinical laboratory response to the 2009 H1N1 influenza outbreak
    Randall T Hayden
    Department of Pathology, St JudeChildren s Research Hospital, Memphis, TN 38105 2794, USA
    Arch Pathol Lab Med 134:1671-8. 2010
    ..Laboratory response represented a critical element in the care of affected patients, but little has been published regarding this aspect of the pandemic...
  5. ncbi request reprint Characterization of culture-derived spiral bacteria by 16S ribosomal RNA gene sequence analysis
    R T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    Diagn Microbiol Infect Dis 39:55-9. 2001
    ..Analysis of 16S rDNA or other molecular targets may provide a way to accurately and rapidly characterize isolates that are recalcitrant to speciation...
  6. ncbi request reprint In situ hybridization for the differentiation of Aspergillus, Fusarium, and Pseudallescheria species in tissue section
    R T Hayden
    Children s Research Hospital, Memphis, TN, USA
    Diagn Mol Pathol 12:21-6. 2003
    ..While less sensitive than morphology, ISH is highly accurate and may help to distinguish between organisms that have similar or identical morphologic features by light microscopy...
  7. ncbi request reprint In situ hybridization for the identification of filamentous fungi in tissue section
    R T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee 38103, USA
    Diagn Mol Pathol 11:119-26. 2002
    ..Its primary use lies in the ability to accurately distinguish between organisms that have similar or identical morphologic features by light microscopy...
  8. pmc Multicenter comparison of different real-time PCR assays for quantitative detection of Epstein-Barr virus
    R T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    J Clin Microbiol 46:157-63. 2008
    ....
  9. ncbi request reprint Quantitative real-time PCR detection of adenovirus in clinical blood specimens: a comparison of plasma, whole blood and peripheral blood mononuclear cells
    J Perlman
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN, United States
    J Clin Virol 40:295-300. 2007
    ..Investigators have described the use of whole blood (WB), peripheral blood mononuclear cells (PBMC), serum and plasma but no studies have compared the utility of these different sample types for use in a clinical diagnostic assay...
  10. pmc Multicenter evaluation of a commercial cytomegalovirus quantitative standard: effects of commutability on interlaboratory concordance
    R T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    J Clin Microbiol 51:3811-7. 2013
    ..Use of a common, commutable quantitative standard can improve agreement across different assays; use of a noncommutable calibrator can reduce agreement among laboratories. ..
  11. pmc Comparison of various blood compartments and reporting units for the detection and quantification of Epstein-Barr virus in peripheral blood
    H Hakim
    Department of Infectious Diseases, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105, USA
    J Clin Microbiol 45:2151-5. 2007
    ..The close correlation between copies/ml and copies/microg DNA also suggests that normalization to cell number or genomic DNA in cellular specimens may not be necessary...
  12. pmc Cutaneous infection caused by Macrophomina phaseolina in a child with acute myeloid leukemia
    Ashok Srinivasan
    Department of Oncology, St Jude Children s Research Hospital, 262 Danny Thomas Place, MS 260, Memphis, TN 38105, USA
    J Clin Microbiol 47:1969-72. 2009
    ..The fungus was identified by DNA sequencing using both the internal transcribed spacer and D1/D2 region of the 28S ribosomal DNA gene...
  13. doi request reprint Infections caused by coryneform bacteria in pediatric oncology patients
    Elisabeth E Adderson
    Department of Infectious Diseases, St Jude Children s Research Hospital, Department of Molecular Sciences, University of Tennessee Health Sciences Center, Memphis, TN 38105, USA
    Pediatr Infect Dis J 27:136-41. 2008
    ..Because pediatric experience is limited, we examined the epidemiology and clinical characteristics of these infections in children undergoing cancer therapy...
  14. ncbi request reprint Baseline mannose binding lectin levels may not predict infection among children with leukemia
    Jeffrey E Rubnitz
    Department of Oncology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    Pediatr Blood Cancer 50:866-8. 2008
    ..Additional studies are required to determine whether serial MBL measurements may be valuable for this purpose...
  15. pmc Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus infections in children with cancer
    Ashok Srinivasan
    Department of Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Pediatr Blood Cancer 53:1216-20. 2009
    ..However, the epidemiology of PVL-positive MRSA infections has not been described in children or adults with cancer...
  16. ncbi request reprint Cidofovir for the treatment of adenoviral infection in pediatric hematopoietic stem cell transplant patients
    Usman Yusuf
    Division of Stem Cell Transplantation, Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Transplantation 81:1398-404. 2006
    ..We report our experience with cidofovir (CDV) for treatment of ADV infection in 57 HSCT patients, median age 8 years (range 0.5-26)...
  17. doi request reprint Staphylococcus aureus bacteremia in pediatric patients with cancer
    Ashok Srinivasan
    Department of Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Pediatr Infect Dis J 29:172-4. 2010
    ..Thirty-eight (73%) of the bacteremic episodes were catheter-related. Methicillin resistance was associated with increased catheter removal (P = 0.003), but no increase in complications or adverse outcomes was seen...
  18. pmc Short communication: methicillin-resistant Staphylococcus aureus infections in children and young adults infected with HIV
    Ashok Srinivasan
    Department of Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    AIDS Res Hum Retroviruses 25:1219-24. 2009
    ..PVL-positive MRSA infections were associated with more SSTI ( p=0.043) but not with increased morbidity or a higher risk of complications compared to PVL-negative MRSA infections in children and young adults with HIV...
  19. ncbi request reprint Diagnosis of catheter-related bloodstream infections among pediatric oncology patients lacking a peripheral culture, using differential time to detection
    Aditya H Gaur
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105 2794, USA
    Pediatr Infect Dis J 24:445-9. 2005
    ..Both the pain and inconvenience of PV cultures are undesirable...
  20. ncbi request reprint Acute necrotizing ulcerative gingivitis and bacteremia caused by Stenotrophomonas maltophilia in an immunocompromised host
    Isao Miyairi
    Department of Infectious Diseases, St Jude Children s Research Hospital, University of Tennessee Health Science Center, Memphis, TN 38105, USA
    Pediatr Infect Dis J 24:181-3. 2005
    ..This case highlights the importance of prompt recognition, debridement and appropriate therapy in immunocompromised patients with acute necrotizing ulcerative gingivitis...
  21. pmc Increasing prevalence of nasal and rectal colonization with methicillin-resistant Staphylococcus aureus in children with cancer
    Ashok Srinivasan
    Department of Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105 3678, USA
    Pediatr Blood Cancer 55:1317-22. 2010
    ..Colonization with S. aureus is a risk factor for infection. However, there are few studies that examine colonization and infection with PVL-positive strains of MRSA in cancer patients...
  22. ncbi request reprint Levels of total fungus and Aspergillus on a pediatric hematopoietic stem cell transplant unit
    Mary Elizabeth Hensley
    St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Pediatr Oncol Nurs 21:67-78. 2004
    ..t tests and analysis of variance were used to verify the findings...
  23. ncbi request reprint Optimizing blood culture practices in pediatric immunocompromised patients: evaluation of media types and blood culture volume
    Aditya H Gaur
    Department of Infectious Diseases, St Jude Children s Research Hospital, University of Tennessee Health Science Center, 332 N Lauderdale Street, Memphis, TN 38105 2794, USA
    Pediatr Infect Dis J 22:545-52. 2003
    ..The optimal use of blood cultures to determine the etiology of febrile episodes in neutropenic children has not been well-defined...
  24. pmc Identification of clinical coryneform bacterial isolates: comparison of biochemical methods and sequence analysis of 16S rRNA and rpoB genes
    Elisabeth E Adderson
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    J Clin Microbiol 46:921-7. 2008
    ..The correlation between identifications produced by different classification systems was poor, particularly for clinical isolates...
  25. pmc Inhibition of primary clinical isolates of human parainfluenza virus by DAS181 in cell culture and in a cotton rat model
    B G Jones
    Department of Infectious Diseases, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA Electronic address
    Antiviral Res 100:562-6. 2013
    ..Together, experiments demonstrated that DAS181 inhibited primary clinical isolates of hPIV in vitro and in vivo at doses similar to those previously described for inhibition of laboratory hPIV and influenza virus isolates. ..
  26. ncbi request reprint Rapid immune reconstitution after a reduced-intensity conditioning regimen and a CD3-depleted haploidentical stem cell graft for paediatric refractory haematological malignancies
    Xiaohua Chen
    Division of Bone Marrow Transplantation, St Jude Children s Research Hospital, Memphis, TN, USA
    Br J Haematol 135:524-32. 2006
    ..Rapid T-cell reconstitution, retention of NK-cells in the graft and induction of low grade GvHD may also enhance the potential anti-cancer immune effect...
  27. pmc Multiplexed, real-time PCR for quantitative detection of human adenovirus
    Z Gu
    St Jude Children s Research Hospital, Memphis, Tennessee 38103, USA
    J Clin Microbiol 41:4636-41. 2003
    ..This assay allows rapid, sensitive, and specific quantitation of adenovirus and may have a significant impact on the care of immunocompromised patients at risk for disseminated viral infection...
  28. ncbi request reprint Difference in time to detection: a simple method to differentiate catheter-related from non-catheter-related bloodstream infection in immunocompromised pediatric patients
    Aditya H Gaur
    Department of Infectious Diseases, St Jude Children s Research Hospital, University of Tennessee Health Science Center, Memphis, TN 38105 2794, USA
    Clin Infect Dis 37:469-75. 2003
    ....
  29. ncbi request reprint Association between tea ingestion and invasive Bacillus cereus infection among children with cancer
    C M El Saleeby
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Clin Infect Dis 39:1536-9. 2004
    ..cereus bacteremia. Policies designed to interrupt transmission of this pathogen to susceptible patients should be considered...
  30. doi request reprint Efficacy of intravascular catheter lock solutions containing preservatives in the prevention of microbial colonization
    L E Shenep
    St Jude Children s Research Hospital, Memphis, TN 38105 3678, USA
    J Hosp Infect 79:317-22. 2011
    ..These findings, together with the authors' historical infection control experience, support the use of preservatives in intravenous lock solutions to reduce catheter related infections in patients beyond the neonatal period...
  31. ncbi request reprint Failure of viridans group streptococci causing bacteremia in pediatric oncology patients to express superantigens
    Esobe F Ohuoba
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    J Pediatr Hematol Oncol 28:627-9. 2006
    ..Adjunctive treatment with intravenous immunoglobulin, which is advantageous in streptococcal toxic shock syndrome, may not benefit these patients...
  32. doi request reprint Computer-assisted bar-coding system significantly reduces clinical laboratory specimen identification errors in a pediatric oncology hospital
    Randall T Hayden
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    J Pediatr 152:219-24. 2008
    ..To assess the ability of a bar code-based electronic positive patient and specimen identification (EPPID) system to reduce identification errors in a pediatric hospital's clinical laboratory...
  33. pmc Discordant rise in galactomannan antigenemia in a patient with resolving Aspergillosis, renal failure, and ongoing hemodialysis
    Chadi M El Saleeby
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105 2794, USA
    J Clin Microbiol 43:3560-3. 2005
    ..In patients with renal failure and aspergillosis, rising serum galactomannan levels may not necessarily signify progressive infection...
  34. ncbi request reprint The epidemiology of chronic hepatitis C infection in survivors of childhood cancer: an update of the St Jude Children's Research Hospital hepatitis C seropositive cohort
    Sharon Castellino
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, TN 38105 2794, USA
    Blood 103:2460-6. 2004
    ..Six patients have died; 1 patient with decompensated cirrhosis died of variceal bleeding. Despite a young age at HCV infection, the progression of liver disease in childhood cancer survivors is comparable to that seen in adults...
  35. ncbi request reprint Filtration-based culture methods improve recovery of fungal pathogens in respiratory specimens
    Chadi M El Saleeby
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105 2794, USA
    Diagn Microbiol Infect Dis 56:221-3. 2006
    ..The new method resulted in an 8.3% (P = 0.0039) increase in recovery for all organisms and a 6.4% (P = 0.0391) for Candida species when compared to the conventional culture technique...
  36. doi request reprint Quantitative real-time polymerase chain reaction detection of BK virus using labeled primers
    Zhengming Gu
    Departments of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee 38103, USA
    Arch Pathol Lab Med 134:444-8. 2010
    ..BK virus infections among immunocompromised patients are associated with disease of the kidney or urinary bladder. High viral loads, determined by quantitative polymerase chain reaction (PCR), have been correlated with clinical disease...
  37. ncbi request reprint Cross-reactivity of non-Aspergillus fungal species in the Aspergillus galactomannan enzyme immunoassay
    Jessica R Cummings
    Department of Pathology, St Jude Children s Research Hospital, Memphis, TN 38103, USA
    Diagn Microbiol Infect Dis 59:113-5. 2007
    ..Of 53 fungal isolates, cross-reactivity was observed with 5 non-Aspergillus spp.: Blastomyces dermatitidis, Nigrospora oryzae, Paecilomyces lilacinus, Penicillium chrysogenum, and Trichothecium roseum...
  38. ncbi request reprint In situ hybridization for the identification of yeastlike organisms in tissue section
    R T Hayden
    Mayo Clinic, Rochester, Minnesota 55905, USA
    Diagn Mol Pathol 10:15-23. 2001
    ..Its primary strength lies in the ability to speciate organisms accurately that are too few or atypical to identify based solely on morphologic features...
  39. pmc Direct detection of Legionella species from bronchoalveolar lavage and open lung biopsy specimens: comparison of LightCycler PCR, in situ hybridization, direct fluorescence antigen detection, and culture
    R T Hayden
    Mayo Clinic, Rochester, Minnesota 55905, USA
    J Clin Microbiol 39:2618-26. 2001
    ..WS staining, Legionella genus LC-PCR, and L. pneumophila species-specific ISH were useful as rapid tests with lung tissue...