Douglas Green

Summary

Affiliation: St. Jude Children's Research Hospital
Country: USA

Publications

  1. doi request reprint Stress in biomedical research: six impossible things
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Mol Cell 40:176-8. 2010
  2. doi request reprint Pseudokiller, qu'est-ce que c'est?
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38139, USA Electronic address
    Immunity 39:421-2. 2013
  3. pmc Sweet nothings: sensing of sugar metabolites controls T cell function
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Cell Metab 18:7-8. 2013
  4. pmc The pantheon of the fallen: why are there so many forms of cell death?
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN, USA
    Trends Cell Biol 22:555-6. 2012
  5. doi request reprint The end and after: how dying cells impact the living organism
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Immunity 35:441-4. 2011
  6. pmc Cytoplasmic functions of the tumour suppressor p53
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Nature 458:1127-30. 2009
  7. pmc Immunogenic and tolerogenic cell death
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA e mails
    Nat Rev Immunol 9:353-63. 2009
  8. doi request reprint Fas Bim boom!
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Immunity 28:141-3. 2008
  9. ncbi request reprint At the gates of death
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Cancer Cell 9:328-30. 2006
  10. pmc Mitochondria and the autophagy-inflammation-cell death axis in organismal aging
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Science 333:1109-12. 2011

Research Grants

  1. Activation-Induced Cell Death in T Cells
    Douglas R Green; Fiscal Year: 2010
  2. Central mechanisms of cell death
    Douglas R Green; Fiscal Year: 2011
  3. CENTRAL MECHANISMS OF APOPTOSIS IN THE IMMUNE SYSTEM
    Douglas Green; Fiscal Year: 2006
  4. Conference on Cellular Senescene and Cell Death
    Douglas Green; Fiscal Year: 2005
  5. Heat Shock-Induced Apoptosis
    Douglas Green; Fiscal Year: 2007
  6. Mechanisms of AICD and Peripheral Deletion in T Cells
    Douglas Green; Fiscal Year: 2007
  7. Mechanisms of Stress Induced Apoptosis in T-Cells
    Douglas Green; Fiscal Year: 2007
  8. Activation and Function of Caspase-2
    Douglas R Green; Fiscal Year: 2010
  9. Central mechanisms of cell death
    Douglas R Green; Fiscal Year: 2010
  10. Heat Shock-Induced Apoptosis
    Douglas Green; Fiscal Year: 2009

Collaborators

Detail Information

Publications83

  1. doi request reprint Stress in biomedical research: six impossible things
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Mol Cell 40:176-8. 2010
    ..We consider six impossible things and what we might be able to do about them, provided they are believed by lunchtime or, with practice, before breakfast...
  2. doi request reprint Pseudokiller, qu'est-ce que c'est?
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38139, USA Electronic address
    Immunity 39:421-2. 2013
    ..Necroptosis is mediated by engagement of RIP kinases and a downstream pseudokinase, MLKL. In this issue of Immunity, Murphy et al. (2013) show that it operates at or close to the final execution mechanism of the death process. ..
  3. pmc Sweet nothings: sensing of sugar metabolites controls T cell function
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Cell Metab 18:7-8. 2013
    ..Now, Chang et al. (2013) in Cell provide a mechanism whereby available sugars dictate metabolic pathways in activated T cells and direct a nonmetabolic regulatory function of glyceraldehyde-3-phosphate dehydrogenase. ..
  4. pmc The pantheon of the fallen: why are there so many forms of cell death?
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN, USA
    Trends Cell Biol 22:555-6. 2012
    ..Here, we attempt to explain why there are so many different forms of cell death, and propose a distinction between active death that constitutes 'suicide' versus 'sabotage'...
  5. doi request reprint The end and after: how dying cells impact the living organism
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Immunity 35:441-4. 2011
    ..Here we discuss the different modes of cell death as they relate to this rapidly evolving field...
  6. pmc Cytoplasmic functions of the tumour suppressor p53
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Nature 458:1127-30. 2009
    ..An emerging area of research unravels additional activities of p53 in the cytoplasm, where it triggers apoptosis and inhibits autophagy. These previously unknown functions contribute to the mission of p53 as a tumour suppressor...
  7. pmc Immunogenic and tolerogenic cell death
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA e mails
    Nat Rev Immunol 9:353-63. 2009
    ..Consequently, a central problem in immunology is to understand how the immune system determines whether cell death is immunogenic, tolerogenic or 'silent'...
  8. doi request reprint Fas Bim boom!
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Immunity 28:141-3. 2008
    ..New findings by Hughes et al. (2008), Hutcheson et al. (2008), and Weant et al. (2008) highlight the roles of apoptosis regulators Bim and Fas in the contraction phase of T cell responses and reveal consequences of failure of this process...
  9. ncbi request reprint At the gates of death
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Cancer Cell 9:328-30. 2006
    ..Here, we discuss an emerging view, proposed by Certo et al. in this issue of Cancer Cell, on how these interactions result in MOMP and apoptosis...
  10. pmc Mitochondria and the autophagy-inflammation-cell death axis in organismal aging
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Science 333:1109-12. 2011
    ..Thus, a combination of mitochondrial dysfunction and insufficient autophagy may contribute to multiple aging-associated pathologies...
  11. pmc A survivor hits the breaks
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Mol Cell 29:411-2. 2008
    ..In this issue of Molecular Cell, Wang et al. (2008) propose another function for Bcl2: the inhibition of DNA repair by nonhomologous end-joining...
  12. ncbi request reprint Life, death, BH3 profiles, and the salmon mousse
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Cancer Cell 12:97-9. 2007
    ....
  13. ncbi request reprint p53 and metabolism: Inside the TIGAR
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Cell 126:30-2. 2006
    ..The discovery of three new target genes for p53 reveals unexpected functions for this tumor suppressor in the regulation of glucose metabolism and autophagy...
  14. pmc RIPK-dependent necrosis and its regulation by caspases: a mystery in five acts
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Mol Cell 44:9-16. 2011
    ..In this review, we will lay out the evidence as it now stands, reinterpreting earlier observations in light of new clues and considering where the investigation might lead...
  15. ncbi request reprint Cell competition: pirates on the tangled bank
    Douglas R Green
    St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Cell Stem Cell 6:287-8. 2010
    ..Two recent studies highlight competition between hematopoietic stem cells based on p53. These findings have implications for both normal homeostasis and tumorigenesis...
  16. pmc Eating for good health: linking autophagy and phagocytosis in host defense
    Miguel A Sanjuan
    Department of Immunology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Autophagy 4:607-11. 2008
    ..Possible links between these two forms of cellular "eating" represent a new dimension in host defense...
  17. pmc Resistance to caspase-independent cell death requires persistence of intact mitochondria
    Stephen W G Tait
    Department of Immunology, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA
    Dev Cell 18:802-13. 2010
    ..Thus, iMOMP provides a critical source of intact mitochondria that permits cellular survival following MOMP...
  18. pmc Pharmacological manipulation of cell death: clinical applications in sight?
    Douglas R Green
    Department of Immunology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Clin Invest 115:2610-7. 2005
    ....
  19. pmc NF-kappaB inhibits T-cell activation-induced, p73-dependent cell death by induction of MDM2
    Valere Busuttil
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 107:18061-6. 2010
    ..Our results demonstrate a pathway for survival of activated T cells through NF-κB-induced Mdm2, which blocks Bim-dependent apoptosis through binding and inhibition of p73...
  20. pmc Characterization of cytoplasmic caspase-2 activation by induced proximity
    Lisa Bouchier-Hayes
    Department of Immunology, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA
    Mol Cell 35:830-40. 2009
    ..Furthermore, we identify Hsp90alpha as a key negative regulator of heat shock-induced caspase-2 activation...
  21. pmc PUMA cooperates with direct activator proteins to promote mitochondrial outer membrane permeabilization and apoptosis
    Jerry E Chipuk
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Cell Cycle 8:2692-6. 2009
    ..Here we will define these interactions and discuss our experiments that suggest PUMA cooperates with direct activator proteins to efficiently induce MOMP and apoptosis...
  22. doi request reprint Mitochondria and cell death: outer membrane permeabilization and beyond
    Stephen W G Tait
    Department of Immunology, St Jude Childrens Research Hospital, Memphis, Tennessee 38105, USA
    Nat Rev Mol Cell Biol 11:621-32. 2010
    ..MOMP typically leads to cell death irrespective of caspase activity by causing a progressive decline in mitochondrial function, although cells can survive this under certain circumstances, which may have pathophysiological consequences...
  23. pmc How do BCL-2 proteins induce mitochondrial outer membrane permeabilization?
    Jerry E Chipuk
    St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105, USA
    Trends Cell Biol 18:157-64. 2008
    ....
  24. pmc Mechanism of apoptosis induction by inhibition of the anti-apoptotic BCL-2 proteins
    Jerry E Chipuk
    Department of Immunology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Proc Natl Acad Sci U S A 105:20327-32. 2008
    ..Our data show that the induction of apoptosis by inhibition of the anti-apoptotic BCL-2 repertoire requires "covert" levels of direct activators of BAX and BAK at the OMM...
  25. pmc Different mitochondrial intermembrane space proteins are released during apoptosis in a manner that is coordinately initiated but can vary in duration
    Cristina Munoz-Pinedo
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 103:11573-8. 2006
    ..The timing and extent of AIF release makes it unlikely that it is involved in the induction of apoptosis, either upstream or downstream of mitochondrial outer membrane permeabilization...
  26. pmc Measuring apoptosis at the single cell level
    Lisa Bouchier-Hayes
    Department of Immunology, St Jude Children s Research Hospital, 332 N Lauderdale Street, Memphis, TN 38105, USA
    Methods 44:222-8. 2008
    ..We discuss these techniques here and highlight the advantages and drawbacks of using such approaches to study apoptosis...
  27. pmc The BCL-2 family reunion
    Jerry E Chipuk
    Department of Immunology, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA
    Mol Cell 37:299-310. 2010
    ..Here we will discuss the mechanisms and functions of the BCL-2 family in the context of these pathways, highlighting the complex integration and regulation of the BCL-2 family in cell fate decisions...
  28. ncbi request reprint Toll-like receptor signalling in macrophages links the autophagy pathway to phagocytosis
    Miguel A Sanjuan
    Department of Immunology, St Jude Children s Research Institute, Memphis, Tennessee 38105, USA
    Nature 450:1253-7. 2007
    ....
  29. doi request reprint Mitochondria in cell death
    MELISSA J PARSONS
    Department of Immunology, St Jude Children s Research Hospital, 262 Danny Thomas Place Mail Stop 351, Memphis, TN 38105, USA
    Essays Biochem 47:99-114. 2010
    ..Proper regulation of these mitochondrial functions is vitally important for the life and death of the cell and for the organism as a whole...
  30. doi request reprint Toll-like receptor signaling in the lysosomal pathways
    Miguel A Sanjuan
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105 3678, USA
    Immunol Rev 227:203-20. 2009
    ..In this review, we discuss recent views on how Toll-like receptors direct microbes to final destruction by regulating the different pathways that lead to the lysosome...
  31. pmc Inducible dimerization and inducible cleavage reveal a requirement for both processes in caspase-8 activation
    Andrew Oberst
    Department of Immunology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 285:16632-42. 2010
    ....
  32. pmc PB1-F2 proteins from H5N1 and 20 century pandemic influenza viruses cause immunopathology
    Julie L McAuley
    Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee, United States of America
    PLoS Pathog 6:e1001014. 2010
    ..These data suggest that the PB1-F2 protein contributes to the virulence of pandemic strains when the PB1 gene segment is recently derived from the avian reservoir...
  33. doi request reprint Live to dead cell imaging
    Stephen W G Tait
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN, USA
    Methods Mol Biol 559:33-48. 2009
    ..Next, the cells are induced to undergo apoptosis and continuously imaged. Finally, quantitative kinetic analysis of various apoptotic processes is performed postimaging...
  34. ncbi request reprint Bid, Bax, and lipids cooperate to form supramolecular openings in the outer mitochondrial membrane
    Tomomi Kuwana
    La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Cell 111:331-42. 2002
    ..We conclude that mitochondrial protein release in apoptosis can be mediated by supramolecular openings in the outer mitochondrial membrane, promoted by BH3/Bax/lipid interaction and directly inhibited by Bcl-x(L)...
  35. ncbi request reprint The pathophysiology of mitochondrial cell death
    Douglas R Green
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Science 305:626-9. 2004
    ..The general rules governing the pathophysiology of MOMP and controversial issues regarding its regulation are discussed...
  36. ncbi request reprint Pharmacologic activation of p53 elicits Bax-dependent apoptosis in the absence of transcription
    Jerry E Chipuk
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
    Cancer Cell 4:371-81. 2003
    ..Hence, pharmacologic p53 modulators can activate a transcription-independent apoptotic program...
  37. ncbi request reprint Minimal BH3 peptides promote cell death by antagonizing anti-apoptotic proteins
    Carole Moreau
    INSERM U419, Nantes, France
    J Biol Chem 278:19426-35. 2003
    ..Thus, BH3 domains do not necessarily activate multidomain pro-apoptotic proteins directly but promote apoptosis by releasing active multidomain pro-apoptotic proteins from their anti-apoptotic counterparts...
  38. ncbi request reprint Disruption of mitochondrial function during apoptosis is mediated by caspase cleavage of the p75 subunit of complex I of the electron transport chain
    Jean Ehrland Ricci
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Cell 117:773-86. 2004
    ..Therefore, caspase cleavage of NDUFS1 is required for several mitochondrial changes associated with apoptosis...
  39. ncbi request reprint And all of a sudden it's over: mitochondrial outer-membrane permeabilization in apoptosis
    Nigel J Waterhouse
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, California 92121, USA
    Biochimie 84:113-21. 2002
    ..It is still unclear how these proteins are released from the mitochondria. Recent advances in our knowledge of mitochondrial outer-membrane permeabilization and the consequences of this event on mitochondria will be discussed...
  40. pmc The role of ARK in stress-induced apoptosis in Drosophila cells
    Katja C Zimmermann
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
    J Cell Biol 156:1077-87. 2002
    ..These results demonstrate the central role of ARK in stress-induced apoptosis, which appears to act independently of cytochrome c. Apoptosis induced by Reaper or Grim can proceed via a distinct pathway, independent of ARK...
  41. ncbi request reprint Cytoplasmic p53: bax and forward
    Jerry E Chipuk
    La Jolla Institute for Allergy and Immunology, Division of Cellular Immunology, San Diego, California 92121, USA
    Cell Cycle 3:429-31. 2004
    ..In parallel, p53 can release pro-apoptotic Bcl-2 proteins sequestered by Bcl-x(L). These data suggest that cytoplasmic p53 functions analogously to the BH3-only proteins, a subset of pro-apoptotic Bcl-2 proteins...
  42. ncbi request reprint Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1
    Ulrich Maurer
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, California 92121, USA
    Mol Cell 21:749-60. 2006
    ..The results demonstrate that the control of MCL-1 stability by GSK-3 is an important mechanism for the regulation of apoptosis by growth factors, PI3K, and AKT...
  43. ncbi request reprint Connected to death: the (unexpurgated) mitochondrial pathway of apoptosis
    Diana Spierings
    La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Science 310:66-7. 2005
    ....
  44. ncbi request reprint BH3 domains of BH3-only proteins differentially regulate Bax-mediated mitochondrial membrane permeabilization both directly and indirectly
    Tomomi Kuwana
    La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Mol Cell 17:525-35. 2005
    ..In this model, the simple inhibition of antiapoptotic functions is insufficient to induce apoptosis unless a direct activator of Bax or Bak is present...
  45. pmc Apoptosis: Stabbed in the BAX
    Douglas R Green
    Nature 455:1047-9. 2008
  46. ncbi request reprint PUMA couples the nuclear and cytoplasmic proapoptotic function of p53
    Jerry E Chipuk
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Science 309:1732-5. 2005
    ..Mutant Bcl-xL that bound p53, but not PUMA, rendered cells resistant to p53-induced apoptosis irrespective of PUMA expression. Thus, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53...
  47. ncbi request reprint Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis
    Jerry E Chipuk
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Science 303:1010-4. 2004
    ..We propose that when p53 accumulates in the cytosol, it can function analogously to the BH3-only subset of proapoptotic Bcl-2 proteins to activate Bax and trigger apoptosis...
  48. doi request reprint Cytochrome c: functions beyond respiration
    Yong Ling P Ow
    The Campbell Family Institute for Breast Cancer Research, Ontario Cancer Institute, Toronto, Ontario M5G 2C1, Canada
    Nat Rev Mol Cell Biol 9:532-42. 2008
    ..As well as its role in canonical intrinsic apoptosis, cytochrome c amplifies signals that are generated by other apoptotic pathways and participates in certain non-apoptotic functions...
  49. ncbi request reprint Mitochondria, apoptosis and autoimmunity
    Michael J Pinkoski
    Medical Research Council, Toxicology Unit, Leicester, UK
    Curr Dir Autoimmun 9:55-73. 2006
    ....
  50. pmc Induction of immunological tolerance by apoptotic cells requires caspase-dependent oxidation of high-mobility group box-1 protein
    Hirotaka Kazama
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 631101, USA
    Immunity 29:21-32. 2008
    ..Similarly, blocking sites of oxidation in HMGB1 prevented tolerance induction by apoptotic cells. These results suggest that caspase-orchestrated mitochondrial events determine the impact of apoptotic cells on the immune response...
  51. pmc Nonlymphoid Fas ligand in peptide-induced peripheral lymphocyte deletion
    Michael J Pinkoski
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
    Proc Natl Acad Sci U S A 99:16174-9. 2002
    ..These data demonstrate that nonlymphoid FasL is expressed in response to peripheral T cell activation and participates in the regulation of T cells that infiltrate peripheral tissues...
  52. pmc Chk1 suppresses a caspase-2 apoptotic response to DNA damage that bypasses p53, Bcl-2, and caspase-3
    Samuel Sidi
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell 133:864-77. 2008
    ..The evolutionarily conserved "Chk1-suppressed" pathway defines a novel apoptotic process, whose responsiveness to Chk1 inhibitors and insensitivity to p53 and BCL2 alterations have important implications for cancer therapy...
  53. pmc Chemical inhibition of the mitochondrial division dynamin reveals its role in Bax/Bak-dependent mitochondrial outer membrane permeabilization
    Ann Cassidy-Stone
    Section of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA
    Dev Cell 14:193-204. 2008
    ..Our findings raise the interesting possibility that mdivi-1 represents a class of therapeutics for stroke, myocardial infarction, and neurodegenerative diseases...
  54. ncbi request reprint Galectin-7 (PIG1) exhibits pro-apoptotic function through JNK activation and mitochondrial cytochrome c release
    Ichiro Kuwabara
    Divisions of Allergy and Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
    J Biol Chem 277:3487-97. 2002
    ..Some of them are potentially contributory to this lectin's proapoptotic function and these include redox-related genes monoamine oxidase B, ryanodine receptor 2, and glutathione S-transferase Mu 3...
  55. pmc Apaf-1 and caspase-9 do not act as tumor suppressors in myc-induced lymphomagenesis or mouse embryo fibroblast transformation
    Clare L Scott
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
    J Cell Biol 164:89-96. 2004
    ..Moreover, loss of Apaf-1 did not promote oncogene-induced transformation of mouse embryo fibroblasts. Thus, Apaf-1 and caspase-9 do not suppress c-myc-induced lymphomagenesis and embryo fibroblast transformation...
  56. ncbi request reprint p53's believe it or not: lessons on transcription-independent death
    Jerry E Chipuk
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, California 92121, USA
    J Clin Immunol 23:355-61. 2003
    ..Here we will explore what is currently known about TIPA learned from various p53 mutants and truncations, along with discussing several proposed mechanisms...
  57. ncbi request reprint Introduction: apoptosis in the development and function of the immune system
    Douglas R Green
    Semin Immunol 15:121-3. 2003
  58. ncbi request reprint RelB/p50 dimers are differentially regulated by tumor necrosis factor-alpha and lymphotoxin-beta receptor activation: critical roles for p100
    Emmanuel Derudder
    Laboratoire Oncogenese, Differenciation et Transduction du Signal, CNRS UPR 9079, Institut Andre Lwoff, 7 rue Guy Moquet, 94801 Villejuif, France
    J Biol Chem 278:23278-84. 2003
    ....
  59. ncbi request reprint Death and NF-kappaB in T cell activation: life at the edge
    Douglas R Green
    La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Mol Cell 11:551-2. 2003
    ..Nuclear Factor kappa-B appears to be required to prevent p73-induced apoptosis triggered by primary T cell activation. Here we explore what this state of affairs might do for the regulation of the immune system...
  60. ncbi request reprint Granzyme A: the road less traveled
    Michael J Pinkoski
    Nat Immunol 4:106-8. 2003
  61. pmc Caspase-mediated loss of mitochondrial function and generation of reactive oxygen species during apoptosis
    Jean Ehrland Ricci
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
    J Cell Biol 160:65-75. 2003
    ....
  62. ncbi request reprint Tumor necrosis factor alpha up-regulates non-lymphoid Fas-ligand following superantigen-induced peripheral lymphocyte activation
    Michael J Pinkoski
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
    J Biol Chem 277:42380-5. 2002
    ....
  63. ncbi request reprint A matter of life and death
    Douglas R Green
    La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, California 92121, USA
    Cancer Cell 1:19-30. 2002
    ..The secondary traits of diverse neoplasms are a consequence of cell proliferation, tissue expansion, and other outcomes of this platform...
  64. ncbi request reprint Uptake of apoptotic antigen-coupled cells by lymphoid dendritic cells and cross-priming of CD8(+) T cells produce active immune unresponsiveness
    Thomas A Ferguson
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA
    J Immunol 168:5589-95. 2002
    ..The results provide a mechanism for a well-established method of inducing immunologic unresponsiveness...
  65. ncbi request reprint Remodeling for demolition: changes in mitochondrial ultrastructure during apoptosis
    John C Reed
    The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 9:1-3. 2002
    ..Both events are induced by proapoptotic members of the Bcl-2 family and appear to be separable...
  66. ncbi request reprint Death receptors bind SHP-1 and block cytokine-induced anti-apoptotic signaling in neutrophils
    Isabelle Daigle
    Swiss Institute of Allergy and Asthma Research SIAF, Davos, Switzerland
    Nat Med 8:61-7. 2002
    ..Thus, we provide molecular and functional evidence for negative signaling by death receptors...
  67. ncbi request reprint Role of Bcl-2 family members in immunity and disease
    Nathalie M Droin
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Biochim Biophys Acta 1644:179-88. 2004
    ..In this review, we describe current knowledge of the functions of different Bcl-2 members and their potential roles in disease and immunity...
  68. ncbi request reprint Regulation of activation-induced Fas (CD95/Apo-1) ligand expression in T cells by the cyclin B1/Cdk1 complex
    Ralph Torgler
    Division of Immunopathology, Institute of Pathology, University of Bern, 3010 Berne, Switzerland
    J Biol Chem 279:37334-42. 2004
    ..In conclusion, our data support a link between cell cycle progression, activation-induced Fas ligand expression, and apoptosis in T cells...
  69. ncbi request reprint The role of p53 and Fas in a model of acute murine graft-versus-host disease
    Shinichiro Yada
    Division of Gastroenterology, Department of Medicine, Northwestern Medical School, Chicago IL 60611
    J Immunol 174:1291-7. 2005
    ..Our data indicate that BM depletion in acute GVHD is mediated by p53-dependent up-regulation of Fas on BMC, which leads to Fas-dependent depletion and subsequent disease...
  70. ncbi request reprint Correlated three-dimensional light and electron microscopy reveals transformation of mitochondria during apoptosis
    Mei G Sun
    Department of Biology, San Diego State University, San Diego, CA 92182 4614, USA
    Nat Cell Biol 9:1057-65. 2007
    ..Swelling occurs only late in apoptosis after release of cytochrome c and loss of the mitochondrial membrane potential...
  71. ncbi request reprint GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation
    Anna Colell
    Department of Cell Death and Proliferation, Institut d Investigacions Biomediques de Barcelona IIBB CSIC, IDIBAPS, 08036 Barcelona, Spain
    Cell 129:983-97. 2007
    ..Therefore, GAPDH mediates an elevation in glycolysis and enhanced autophagy that cooperate to protect cells from CICD...
  72. ncbi request reprint Duration of CTL activation regulates IL-2 production required for autonomous clonal expansion
    Diana C J Spierings
    La Jolla Institute for Allergy and Immunology, San Diego, CA, USA
    Eur J Immunol 36:1707-17. 2006
    ..These results indicate that the nature and duration of antigenic stimulation regulate the autonomy of CTL expansion via the production of autocrine IL-2...
  73. pmc Caspase-2-induced apoptosis requires bid cleavage: a physiological role for bid in heat shock-induced death
    Christine Bonzon
    Department of Molecular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
    Mol Biol Cell 17:2150-7. 2006
    ..The data are consistent with a model in which Caspase-2 induces apoptosis via cleavage of Bid at D59 and the subsequent engagement of the mitochondrial (intrinsic) pathway...
  74. ncbi request reprint In situ trapping of activated initiator caspases reveals a role for caspase-2 in heat shock-induced apoptosis
    Shine Tu
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Dr, San Diego, CA 92121, USA
    Nat Cell Biol 8:72-7. 2006
    ..This approach unambiguously identifies the apical caspase activated in response to apoptotic stimuli, and establishes caspase-2 as a proximal mediator of heat shock-induced apoptosis...
  75. pmc The multidomain proapoptotic molecules Bax and Bak are directly activated by heat
    Lisa J Pagliari
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
    Proc Natl Acad Sci U S A 102:17975-80. 2005
    ..Our results support an emerging paradigm, wherein the activation of Bax or Bak and the blockade of antiapoptotic Bcl-2 proteins are pivotal steps in the mitochondrial pathway of apoptosis...
  76. ncbi request reprint Dynamic regulation of FoxP3 expression controls the balance between CD4+ T cell activation and cell death
    Deborah J Kasprowicz
    Benaroya Research Institute at Virginia Mason, Seattle, WA 98101, USA
    Eur J Immunol 35:3424-32. 2005
    ..Concomitant with differentiation is a loss of FoxP3 mRNA and protein. These data demonstrate that FoxP3 levels regulate T cell function, and that FoxP3 itself is dynamically regulated during effector T cell differentiation...
  77. pmc A role for transferrin receptor in triggering apoptosis when targeted with gambogic acid
    Shailaja Kasibhatla
    Maxim Pharmaceuticals, Inc, 6650 Nancy Ridge Drive, San Diego, CA 92121, USA
    Proc Natl Acad Sci U S A 102:12095-100. 2005
    ..In summary, GA binding to TfR induces a unique signal leading to rapid apoptosis of tumor cells. These results suggest that GA may provide an additional approach for targeting the TfR and its use in cancer therapy...
  78. ncbi request reprint Life support: the alpha4 phosphatase subunit in cell survival and apoptosis
    Maya Saleh
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
    Trends Cell Biol 15:285-7. 2005
    ....
  79. ncbi request reprint Apoptotic pathways: ten minutes to dead
    Douglas R Green
    La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
    Cell 121:671-4. 2005
    ..The emerging, if preliminary, perspective these new studies provide may represent either a refinement of our views of how cells die or, perhaps, the beginnings of what amounts to a reformulation of our ideas...
  80. ncbi request reprint CD4+ T-cell help controls CD8+ T-cell memory via TRAIL-mediated activation-induced cell death
    Edith M Janssen
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, California 92121, USA
    Nature 434:88-93. 2005
    ..Regulation of Trail expression can therefore account for the role of CD4+ T cells in the generation of CD8+ T cell memory and represents a novel mechanism for controlling adaptive immune responses...
  81. ncbi request reprint Do inducers of apoptosis trigger caspase-independent cell death?
    Jerry E Chipuk
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, California 92121, USA
    Nat Rev Mol Cell Biol 6:268-75. 2005
    ..Here, we present recent findings and discuss the physiological relevance of caspase-independent cell death...
  82. pmc The ubiquitin-protein ligase Itch regulates p73 stability
    Mario Rossi
    Department of Biology, University of Rome Tor Vergata, Rome, Italy
    EMBO J 24:836-48. 2005
    ..In conclusion, we have identified a key mechanism in the control of p73 protein levels both in normal as well as in stress conditions...
  83. ncbi request reprint Lymphocyte apoptosis: refining the paths to perdition
    Michael J Pinkoski
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
    Curr Opin Hematol 9:43-9. 2002
    ..In addition, the intricacies of the mitochondrial role in apoptosis are starting to unravel with reports of novel proteins, such as Smac/DIABLO, and old proteins, such as heat shock proteins, playing new roles in regulating cell death...

Research Grants51

  1. Activation-Induced Cell Death in T Cells
    Douglas R Green; Fiscal Year: 2010
    ..This novel pathway has fundamental implications for our understanding of T lymphocyte responses under different conditions, and will have impact on our ability to manipulate the immune response. ..
  2. Central mechanisms of cell death
    Douglas R Green; Fiscal Year: 2011
    ..Successful completion of the proposed work will reveal fundamental new processes in the control of cell life and death. ..
  3. CENTRAL MECHANISMS OF APOPTOSIS IN THE IMMUNE SYSTEM
    Douglas Green; Fiscal Year: 2006
    ..Our studies will elucidate how the central mechanisms of this mitochondrial-caspase axis control life/death decisions in cells. ..
  4. Conference on Cellular Senescene and Cell Death
    Douglas Green; Fiscal Year: 2005
    ..The interconnections between these biological events and the overlapping principles that emerge represent an emerging field in biology and biomedical research. ..
  5. Heat Shock-Induced Apoptosis
    Douglas Green; Fiscal Year: 2007
    ..These three goals represent independent and complimentary approaches to the elucidation of the novel apoptotic pathway represented by heat shock and proceeding through the activation of caspase-2 ..
  6. Mechanisms of AICD and Peripheral Deletion in T Cells
    Douglas Green; Fiscal Year: 2007
    ..Our goals represent an integrated approach to understanding the processes of AICD and peripheral deletion and their relationships to the pathways of apoptosis. ..
  7. Mechanisms of Stress Induced Apoptosis in T-Cells
    Douglas Green; Fiscal Year: 2007
    ..In this integrated approach to the problem of p53 function in apoptosis, we will elucidate the roles of p53 in the context of stress-induced apoptosis in mature and developing T cells. ..
  8. Activation and Function of Caspase-2
    Douglas R Green; Fiscal Year: 2010
    ..The studies proposed herein will provide mechanistic insights into these functions. ..
  9. Central mechanisms of cell death
    Douglas R Green; Fiscal Year: 2010
    ..Successful completion of the proposed work will reveal fundamental new processes in the control of cell life and death. ..
  10. Heat Shock-Induced Apoptosis
    Douglas Green; Fiscal Year: 2009
    ..These three goals represent independent and complimentary approaches to the elucidation of the novel apoptotic pathway represented by heat shock and proceeding through the activation of caspase-2 ..
  11. THE ANTI-APOPTOTIC ROLE OF HEATSHOCK PROTEIN 70 (HSP 70)
    Douglas Green; Fiscal Year: 2004
    ..Together, our studies will secure a mechanistic basis for the function of Hsp70 in the regulation of cell death and cell survival. ..
  12. PERIPHERAL T CELL DELETION BY NONLYMPHOID FAS LIGAND
    Douglas Green; Fiscal Year: 2003
    ..Together, these studies will give us insights into the mechanisms whereby T cells induce expression of peripheral, nonlymphoid FasL and the roles this plays in immune homeostasis. ..
  13. MECHANISMS OF STRESS-INDUCED APOPTOSIS IN T CELLS
    Douglas Green; Fiscal Year: 2001
    ..His focus on T lymphocytes is important in the context of understanding how the immune system responds to DNA damage and related stress to remove potentially damaged cells, that could otherwise pose a threat to immune integrity. ..
  14. CENTRAL MECHANISMS OF APOPTOSIS IN THE IMMUNE SYSTEM
    Douglas Green; Fiscal Year: 2001
    ..Once characterized in detail, the activation of the critical proteases will eventually serve as a biochemical thread by which to trace the molecular events that begin at the surface and result in cell death in the immune system. ..