R E Lanford

Summary

Affiliation: Southwest Foundation for Biomedical Research
Country: USA

Publications

  1. pmc Therapeutic silencing of microRNA-122 in primates with chronic hepatitis C virus infection
    Robert E Lanford
    Department of Virology and Immunology and Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, TX 78227, USA
    Science 327:198-201. 2010
  2. ncbi request reprint Comparison of tamarins and marmosets as hosts for GBV-B infections and the effect of immunosuppression on duration of viremia
    Robert E Lanford
    Department of Virology and Immunology, Southwest National Primate Research Center, and Southwest Foundation for Biomedical Research, San Antonio, TX 78227, USA
    Virology 311:72-80. 2003
  3. pmc Antiviral effect and virus-host interactions in response to alpha interferon, gamma interferon, poly(i)-poly(c), tumor necrosis factor alpha, and ribavirin in hepatitis C virus subgenomic replicons
    Robert E Lanford
    Department of Virology and Immunology, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 77:1092-104. 2003
  4. ncbi request reprint Advances in model systems for hepatitis C virus research
    Robert E Lanford
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    Virology 293:1-9. 2002
  5. pmc Ribavirin induces error-prone replication of GB virus B in primary tamarin hepatocytes
    R E Lanford
    Department of Virology and Immunology, Southwest Regional Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 75:8074-81. 2001
  6. ncbi request reprint Infectious cDNA clone of the hepatitis C virus genotype 1 prototype sequence
    R E Lanford
    Department of Virology and Immunology, Southwest Regional Primate Research Center, Southwest Foundation for Biomedical Research, 7620 NW Loop 410, San Antonio, TX 78227, USA
    J Gen Virol 82:1291-7. 2001
  7. pmc Hepadnavirus infection in captive gibbons
    R E Lanford
    Department of Virology and Immunology, Southwest Regional Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 74:2955-9. 2000
  8. pmc Mapping of the hepatitis B virus reverse transcriptase TP and RT domains by transcomplementation for nucleotide priming and by protein-protein interaction
    R E Lanford
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 73:1885-93. 1999
  9. pmc Cross-genotype immunity to hepatitis C virus
    Robert E Lanford
    Department of Virology and Immunology, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 78:1575-81. 2004
  10. ncbi request reprint Genomic response to interferon-alpha in chimpanzees: implications of rapid downregulation for hepatitis C kinetics
    Robert E Lanford
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, Southwest National Primate Research Center, San Antonio, TX 78227, USA
    Hepatology 43:961-72. 2006

Collaborators

Detail Information

Publications40

  1. pmc Therapeutic silencing of microRNA-122 in primates with chronic hepatitis C virus infection
    Robert E Lanford
    Department of Virology and Immunology and Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, TX 78227, USA
    Science 327:198-201. 2010
    ..The prolonged virological response to SPC3649 treatment without HCV rebound holds promise of a new antiviral therapy with a high barrier to resistance...
  2. ncbi request reprint Comparison of tamarins and marmosets as hosts for GBV-B infections and the effect of immunosuppression on duration of viremia
    Robert E Lanford
    Department of Virology and Immunology, Southwest National Primate Research Center, and Southwest Foundation for Biomedical Research, San Antonio, TX 78227, USA
    Virology 311:72-80. 2003
    ..The greater availability of marmosets in comparison to tamarins will greatly facilitate future research efforts with this model...
  3. pmc Antiviral effect and virus-host interactions in response to alpha interferon, gamma interferon, poly(i)-poly(c), tumor necrosis factor alpha, and ribavirin in hepatitis C virus subgenomic replicons
    Robert E Lanford
    Department of Virology and Immunology, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 77:1092-104. 2003
    ..These studies reveal characteristics of Huh7 cells that may contribute to their unique capacity to support HCV RNA synthesis and demonstrate the utility of the replicon system for mechanistic studies on antiviral agents...
  4. ncbi request reprint Advances in model systems for hepatitis C virus research
    Robert E Lanford
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    Virology 293:1-9. 2002
  5. pmc Ribavirin induces error-prone replication of GB virus B in primary tamarin hepatocytes
    R E Lanford
    Department of Virology and Immunology, Southwest Regional Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 75:8074-81. 2001
    ....
  6. ncbi request reprint Infectious cDNA clone of the hepatitis C virus genotype 1 prototype sequence
    R E Lanford
    Department of Virology and Immunology, Southwest Regional Primate Research Center, Southwest Foundation for Biomedical Research, 7620 NW Loop 410, San Antonio, TX 78227, USA
    J Gen Virol 82:1291-7. 2001
    ..This is the first example of an infectious chimeric HCV clone. An infectious cDNA clone of HCV-1 will be of particular value, since it is the prototype HCV sequence and many commonly used reagents are based on this sequence...
  7. pmc Hepadnavirus infection in captive gibbons
    R E Lanford
    Department of Virology and Immunology, Southwest Regional Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 74:2955-9. 2000
    ..Phylogenetic analysis indicated that the gibbon isolates lie within the human HBV family, indicating that these HBV isolates most likely stem from infection of gibbons from a human source...
  8. pmc Mapping of the hepatitis B virus reverse transcriptase TP and RT domains by transcomplementation for nucleotide priming and by protein-protein interaction
    R E Lanford
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 73:1885-93. 1999
    ..These studies help to further define the complex protein-protein interactions required in HBV genome replication...
  9. pmc Cross-genotype immunity to hepatitis C virus
    Robert E Lanford
    Department of Virology and Immunology, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 78:1575-81. 2004
    ..These studies are the first to show that cross-genotype immunity can be induced to HCV, demonstrating the feasibility of developing a vaccine protective against all HCV strains...
  10. ncbi request reprint Genomic response to interferon-alpha in chimpanzees: implications of rapid downregulation for hepatitis C kinetics
    Robert E Lanford
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, Southwest National Primate Research Center, San Antonio, TX 78227, USA
    Hepatology 43:961-72. 2006
    ..In conclusion, the response to IFN-alpha is largely tissue-specific, and the response is rapidly downregulated in vivo, which may have a significant influence on the kinetics of antiviral response...
  11. pmc Lack of response to exogenous interferon-alpha in the liver of chimpanzees chronically infected with hepatitis C virus
    Robert E Lanford
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78227, USA
    Hepatology 46:999-1008. 2007
    ..The IFNalpha response in chronically HCV-infected chimpanzees may be mechanistically similar to the null response in the human population...
  12. ncbi request reprint Hepatitis C virus genotype 1b chimeric replicon containing genotype 3 NS5A domain
    Robert E Lanford
    Department of Virology and Immunology, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, 7620 NW Loop 410, San Antonio, TX 78227, USA
    Virology 355:192-202. 2006
    ..The results suggest that NS5A is not sufficient to convey the increased IFNalpha response by genotype 3 or the replicon model is not capable of mimicking the events involved in increased sustained viral response...
  13. pmc Transcomplementation of nucleotide priming and reverse transcription between independently expressed TP and RT domains of the hepatitis B virus reverse transcriptase
    R E Lanford
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 71:2996-3004. 1997
    ..The results indicate that using the baculovirus system, it is possible to dissect the protein-protein and protein-RNA interactions required for HBV genome replication...
  14. pmc An infectious clone of woolly monkey hepatitis B virus
    Robert E Lanford
    Department of Virology and Immunology, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, TX 78227, USA
    J Virol 77:7814-9. 2003
    ..These data demonstrate the production of an infectious clone for WMHBV and extend the characterization of the spider monkey animal model...
  15. pmc Isolation of a hepadnavirus from the woolly monkey, a New World primate
    R E Lanford
    Departments of Virology and Immunology, Southwest Foundation for Biomedical Research, 7620 Northwest Loop 410, San Antonio, TX 78227, USA
    Proc Natl Acad Sci U S A 95:5757-61. 1998
    ..Thus, we have isolated a hepadnavirus with a host intermediate between humans and rodents and establishes a new animal model for evaluation of antiviral therapies for treating HBV chronic infections...
  16. ncbi request reprint GB virus B as a model for hepatitis C virus
    B Beames
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research and Southwest Regional Primate Research Center, San Antonio, Texas, USA
    ILAR J 42:152-60. 2001
    ..The acute versus chronic course of infection may point to important differences between the two viruses that, along with the numerous similarities, will make GBV-B in tamarins a good surrogate model for HCV...
  17. pmc Analysis of hepatitis C virus-inoculated chimpanzees reveals unexpected clinical profiles
    S E Bassett
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 72:2589-99. 1998
    ..If extrapolated to the human population, these data would imply that a significant percentage of unrecognized HCV infections may occur and that silent carriers may represent potentially infectious blood donors...
  18. ncbi request reprint The chimpanzee model of hepatitis C virus infections
    R E Lanford
    Department of Virology and Immunology, Southwest Regional Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, Texas, USA
    ILAR J 42:117-26. 2001
    ..The chimpanzee will continue to provide a critical aspect to the understanding of HCV disease and the development of therapeutic modalities...
  19. pmc DNA microarray analysis of chimpanzee liver during acute resolving hepatitis C virus infection
    C B Bigger
    Department of Virology and Immunology, Southwest Regional Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 75:7059-66. 2001
    ..The data suggest a biphasic mechanism of viral clearance dependent on both the innate and adaptive immune responses and provide insight into the response of the liver to a hepatotropic viral infection...
  20. pmc Identification of four nuclear transport signal-binding proteins that interact with diverse transport signals
    L Yamasaki
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas
    Mol Cell Biol 9:3028-36. 1989
    ..The four proteins were not bound by wheat germ agglutinin and were not associated tightly with the nuclear pore complex...
  21. pmc Lack of adaptation of chimeric GB virus B/hepatitis C virus in the marmoset model: possible effects of bottleneck
    Trudie Weatherford
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78227, USA
    J Virol 83:8062-75. 2009
    ..We speculate that the failure of the chimeric virus to adapt in marmosets might be due to a bottleneck that occurs at the time of infection of resistant animals, which may lead to a loss of fitness upon serial passage...
  22. pmc Viral persistence, antibody to E1 and E2, and hypervariable region 1 sequence stability in hepatitis C virus-inoculated chimpanzees
    S E Bassett
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA
    J Virol 73:1118-26. 1999
    ..These results suggest that mechanisms in addition to the emergence of HVR-1 antibody escape variants are involved in maintaining viral persistence. The significance of antibodies to E1 and E2 in the chimpanzee animal model is discussed...
  23. pmc Intrahepatic gene expression during chronic hepatitis C virus infection in chimpanzees
    Catherine B Bigger
    Department of Virology and Immunology, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, 7620 NW Loop 410, San Antonio, TX 78227, USA
    J Virol 78:13779-92. 2004
    ..Gene expression data in concert with other observations from HCV infections permit speculation on the regulation of specific aspects of HCV infection...
  24. pmc Antiviral activity and host gene induction by tamarin and marmoset interferon-alpha and interferon-gamma in the GBV-B primary hepatocyte culture model
    Deborah Chavez
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research and Southwest National Primate Research Center, 7620 NW Loop 410, San Antonio, TX 78227, USA
    Virology 390:186-96. 2009
    ..Since the GBV-B hepatocyte culture model possesses a functional innate immune response, it will provide opportunities to explore the nature of the antiviral response to a virus closely related to HCV...
  25. pmc Analysis of host range phenotypes of primate hepadnaviruses by in vitro infections of hepatitis D virus pseudotypes
    Azeneth Barrera
    Department of Virology and Immunology, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, 7620 NW Loop 410, San Antonio, TX 78227, USA
    J Virol 78:5233-43. 2004
    ..Based upon these data, we discuss the possibility that the L protein may be comprised of two domains that affect infectivity and that sequences downstream of residue 40 may influence host range and receptor binding or entry...
  26. pmc The marmoset model of GB virus B infections: adaptation to host phenotypic variation
    Trudie Weatherford
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78227, USA
    J Virol 83:5806-14. 2009
    ....
  27. pmc Mapping of the hepatitis B virus pre-S1 domain involved in receptor recognition
    Azeneth Barrera
    Department of Virology and Immunology, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, TX 78227, USA
    J Virol 79:9786-98. 2005
    ....
  28. ncbi request reprint Transcomplementation of core and polymerase functions of the woolly monkey and human hepatitis B viruses
    Lisa Lott
    Department of Virology and Immunology, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, TX 78227, USA
    Virology 308:330-9. 2003
    ..These results highlight the conservation of function despite significant sequence divergence in these viruses...
  29. pmc Amplification of simian retroviral sequences from human recipients of baboon liver transplants
    J S Allan
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228, USA
    AIDS Res Hum Retroviruses 14:821-4. 1998
    ..The persistence of SFV and BaEV in human recipients throughout the posttransplant period underscores the potential infectious risks associated with xenotransplantation...
  30. ncbi request reprint Infection of primary chimpanzee hepatocytes with recombinant hepatitis D virus particles: a surrogate model for hepatitis B virus
    Azeneth Barrera
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX, USA
    Methods Mol Med 96:131-42. 2004
  31. ncbi request reprint Comparison of diverse transport signals in synthetic peptide-induced nuclear transport
    R E Lanford
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78284
    Exp Cell Res 186:32-8. 1990
    ..However, the short nucleoplasmin peptide was only marginally more effective than the T antigen mutant peptide when conjugates with a high number of signals coupled per carrier protein were examined...
  32. ncbi request reprint Plasma protein and apolipoprotein synthesis by human yolk sac carcinoma cells in vitro
    R E Lanford
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228
    In Vitro Cell Dev Biol 27:205-10. 1991
    ..The results indicate that these yolk sac carcinoma cells display a protein expression profile similar to that observed for the human yolk sac, and the possibility that the cells may have the potential to differentiate is discussed...
  33. ncbi request reprint Expression and purification of functional hepatitis B virus polymerase in the baculovirus insect cell system
    Lisa Lott
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX, USA
    Methods Mol Med 95:271-9. 2004
  34. ncbi request reprint Signal peptide peptidase cleavage of GB virus B core protein is required for productive infection in vivo
    Paul Targett-Adams
    Medical Research Council Virology Unit, Church Street, Glasgow, G11 5JR, United Kingdom
    J Biol Chem 281:29221-7. 2006
    ..Moreover, they identify SPP as a potential therapeutic target for reducing the impact of HCV infection...
  35. pmc GB virus B disrupts RIG-I signaling by NS3/4A-mediated cleavage of the adaptor protein MAVS
    Zihong Chen
    Department of Microbiology and Immunology, Center for Hepatitis Research, Institute for Human Infections and Immunity, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1019, USA
    J Virol 81:964-76. 2007
    ..These data provide further support for the use of GBV-B infection in small primates as an accurate surrogate model for deciphering virus-host interactions in hepacivirus pathogenesis...
  36. ncbi request reprint A chimeric GB virus B with 5' nontranslated RNA sequence from hepatitis C virus causes hepatitis in tamarins
    Rene Rijnbrand
    Department of Microbiology and Immunology and Institute for Human Infections and Immunity, The University of Texas Medical Branch, Galveston, TX 77555 1019, USA
    Hepatology 41:986-94. 2005
    ..In conclusion, such novel chimeric flaviviruses offer opportunities for new insights into HCV replication mechanisms, while potentially facilitating the evaluation of candidate therapeutics in vivo...
  37. pmc Activity of a potent hepatitis C virus polymerase inhibitor in the chimpanzee model
    Chih Ming Chen
    Abbott Laboratories, Global Pharmaceutical Research and Development, Abbott Park, IL 60064, USA
    Antimicrob Agents Chemother 51:4290-6. 2007
    ..These findings validate the antiviral efficacy and resistance development of benzothiadiazine HCV polymerase inhibitors in vivo...
  38. pmc Chronic hepatitis associated with GB virus B persistence in a tamarin after intrahepatic inoculation of synthetic viral RNA
    Annette Martin
    Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1019, USA
    Proc Natl Acad Sci U S A 100:9962-7. 2003
    ..Mitochondrial structural changes and altered lipid metabolism leading to steatosis are conserved features of the pathogenesis of chronic hepatitis caused by these genetically distinct flaviviruses...
  39. pmc Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro
    Brett D Lindenbach
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 103:3805-9. 2006
    ..These results greatly extend the utility of the HCVcc genetic system to allow the complete in vitro and in vivo dissection of the HCV life cycle...
  40. ncbi request reprint Interferon-alpha2b secretion by adenovirus-mediated gene delivery in rat, rabbit, and chimpanzee results in similar pharmacokinetic profiles
    G William Demers
    Canji, Inc, San Diego, California 92121, USA
    Toxicol Appl Pharmacol 180:36-42. 2002
    ..These studies suggest that serum concentration of secreted transgene products after gene delivery in small animal models may be highly predictive for larger species and will help define dosing strategies in human patients...

Research Grants17

  1. GBV-B: A SMALL PRIMATE MODEL FOR HEPATITIS C INFECTION
    Robert Lanford; Fiscal Year: 2007
    ....
  2. Resolution of Acute Hepatitis B Virus Infections in Chimpanzees
    Robert Lanford; Fiscal Year: 2007
    ..Our long-term objective is to apply our understanding of the resolution of acute HBV infection to the development of effective new immunotherapies for chronic HBV infections. ..
  3. Gene Expression Analyses During IFN Antiviral Therapy
    Robert Lanford; Fiscal Year: 2005
    ..These data will help determine why interferon therapy is often unsuccessful for chronic HCV infections and provide cellular targets for future therapies. ..
  4. GBV-B-- A SMALL PRIMATE MODEL FOR HEPATITIS C INFECTION
    Robert Lanford; Fiscal Year: 2002
    ..It is anticipated that a viable surrogate model for HCV infection will be developed. ..