Gregory Brewer

Summary

Affiliation: Southern Illinois University School of Medicine
Country: USA

Publications

  1. pmc Age-related differences in NFkappaB translocation and Bcl-2/Bax ratio caused by TNFalpha and Abeta42 promote survival in middle-age neurons and death in old neurons
    Jigisha R Patel
    Department of Medical Microbiology, Immunology, and Cell Biology, Southern Illinois University, School of Medicine, Springfield, IL, USA
    Exp Neurol 213:93-100. 2008
  2. pmc Age-related toxicity of amyloid-beta associated with increased pERK and pCREB in primary hippocampal neurons: reversal by blueberry extract
    Gregory J Brewer
    Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, IL 62794 9626, USA
    J Nutr Biochem 21:991-8. 2010
  3. ncbi request reprint Culture and regeneration of human neurons after brain surgery
    G J Brewer
    Molecular Biology, Microbiology and Biochemistry, Southern Illinois University School of Medicine, Springfield, IL 62794 9626, USA
    J Neurosci Methods 107:15-23. 2001
  4. ncbi request reprint Isolation and culture of adult rat hippocampal neurons
    G J Brewer
    Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield 62794 1220, USA
    J Neurosci Methods 71:143-55. 1997
  5. ncbi request reprint Effect of Neuregen nutrient medium on survival of cortical neurons after aspiration lesion in rats
    Gregory J Brewer
    Department of Neurology, Medical Microbiology, and Immunology, Division of Neurosurgery, Southern Illinois University School of Medicine, Springfield, Illinois 62794 9626, USA
    J Neurosurg 98:1291-8. 2003
  6. ncbi request reprint Prevention of age-related dysregulation of calcium dynamics by estrogen in neurons
    Gregory J Brewer
    Department of Neurology, Southern Illinois University School Medicine, P O Box 19626, Springfield, IL 62794 9626, USA
    Neurobiol Aging 27:306-17. 2006
  7. ncbi request reprint Human primary brain tumor cell growth inhibition in serum-free medium optimized for neuron survival
    Gregory J Brewer
    Departments of Neurology, Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, IL 62794 9626, USA
    Brain Res 1157:156-66. 2007
  8. ncbi request reprint Isolation and culture of adult neurons and neurospheres
    Gregory J Brewer
    Department of Neurology, Southern Illinois University School of Medicine, Springfield, Illinois 62794 9626, USA
    Nat Protoc 2:1490-8. 2007
  9. ncbi request reprint Neuronal plasticity and stressor toxicity during aging
    G J Brewer
    Department of Neurology, School of Medicine, Southern Illinois University, Springfield, IL, 62794 9626, USA
    Exp Gerontol 35:1165-83. 2000
  10. ncbi request reprint Protective effect of the energy precursor creatine against toxicity of glutamate and beta-amyloid in rat hippocampal neurons
    G J Brewer
    Department of Medical Microbiology Immunology, Southern Illinois University School of Medicine, Springfield, Illinois 62794 9626, USA
    J Neurochem 74:1968-78. 2000

Research Grants

  1. Age-Dependent Response of Hippocampal Neurons to Stress
    Gregory Brewer; Fiscal Year: 2006
  2. AGE DEPENDENT RESPONSE OF HIPPOCAMPAL NEURONS TO STRESS
    Gregory Brewer; Fiscal Year: 2000
  3. Mitoenergetic Failure in Brain Aging
    Gregory J Brewer; Fiscal Year: 2010

Collaborators

Detail Information

Publications40

  1. pmc Age-related differences in NFkappaB translocation and Bcl-2/Bax ratio caused by TNFalpha and Abeta42 promote survival in middle-age neurons and death in old neurons
    Jigisha R Patel
    Department of Medical Microbiology, Immunology, and Cell Biology, Southern Illinois University, School of Medicine, Springfield, IL, USA
    Exp Neurol 213:93-100. 2008
    ..In the setting of old neurons, these results suggest that overactivated nuclear translocation of NFkappaB and lower Bcl-2 levels promote death that is reduced by inhibition of either TNFR1 or R2...
  2. pmc Age-related toxicity of amyloid-beta associated with increased pERK and pCREB in primary hippocampal neurons: reversal by blueberry extract
    Gregory J Brewer
    Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, IL 62794 9626, USA
    J Nutr Biochem 21:991-8. 2010
    ..These results suggest that the beneficial effects of blueberry extract may involve transient stress signaling and ROS protection that may translate into improved cognition in aging rats and APP/PS1 mice given blueberry extract...
  3. ncbi request reprint Culture and regeneration of human neurons after brain surgery
    G J Brewer
    Molecular Biology, Microbiology and Biochemistry, Southern Illinois University School of Medicine, Springfield, IL 62794 9626, USA
    J Neurosci Methods 107:15-23. 2001
    ..Three postmortem cases did not regenerate neurites. These methods may aid development of human culture models of epilepsy as well as human pharmacology, toxicology and development of improved methods for brain grafts...
  4. ncbi request reprint Isolation and culture of adult rat hippocampal neurons
    G J Brewer
    Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield 62794 1220, USA
    J Neurosci Methods 71:143-55. 1997
    ..They also will enable aging studies. Therefore, the concept of environmental growth restriction may be more appropriate for neurons in the brain than the concept of a genetic block that precludes regeneration of processes...
  5. ncbi request reprint Effect of Neuregen nutrient medium on survival of cortical neurons after aspiration lesion in rats
    Gregory J Brewer
    Department of Neurology, Medical Microbiology, and Immunology, Division of Neurosurgery, Southern Illinois University School of Medicine, Springfield, Illinois 62794 9626, USA
    J Neurosurg 98:1291-8. 2003
    ..The authors hypothesize that brain lesions irrigated and soaked in Neuregen nutrients will have better neuron survival rates in deafferented regions than lesions irrigated with saline...
  6. ncbi request reprint Prevention of age-related dysregulation of calcium dynamics by estrogen in neurons
    Gregory J Brewer
    Department of Neurology, Southern Illinois University School Medicine, P O Box 19626, Springfield, IL 62794 9626, USA
    Neurobiol Aging 27:306-17. 2006
    ..These results demonstrate age-associated dysregulation of [Ca2+]i homeostasis which was largely prevented by 17beta-estradiol with implications for estrogen/hormone therapy...
  7. ncbi request reprint Human primary brain tumor cell growth inhibition in serum-free medium optimized for neuron survival
    Gregory J Brewer
    Departments of Neurology, Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, IL 62794 9626, USA
    Brain Res 1157:156-66. 2007
    ..The possible in vivo efficacy of Neuregen for treatment of brain tumor resections remains to be determined...
  8. ncbi request reprint Isolation and culture of adult neurons and neurospheres
    Gregory J Brewer
    Department of Neurology, Southern Illinois University School of Medicine, Springfield, Illinois 62794 9626, USA
    Nat Protoc 2:1490-8. 2007
    ..This protocol emphasizes two differences between neuron differentiation and neurosphere proliferation: adhesion dependence and the differentiating power of retinyl acetate...
  9. ncbi request reprint Neuronal plasticity and stressor toxicity during aging
    G J Brewer
    Department of Neurology, School of Medicine, Southern Illinois University, Springfield, IL, 62794 9626, USA
    Exp Gerontol 35:1165-83. 2000
    ..Together with newly demonstrated capabilities for adult and aged neuron regeneration and multiplication, i.e. plasticity, these approaches offer new hope toward reversing age-related decrements and damage from neurodegenerative disease...
  10. ncbi request reprint Protective effect of the energy precursor creatine against toxicity of glutamate and beta-amyloid in rat hippocampal neurons
    G J Brewer
    Department of Medical Microbiology Immunology, Southern Illinois University School of Medicine, Springfield, Illinois 62794 9626, USA
    J Neurochem 74:1968-78. 2000
    ..These results suggest that fortified energy reserves are able to protect neurons against important cytotoxic agents. The oral availability of creatine may benefit patients with neurodegenerative diseases...
  11. pmc NbActiv4 medium improvement to Neurobasal/B27 increases neuron synapse densities and network spike rates on multielectrode arrays
    Gregory J Brewer
    Southern Illinois University School of Medicine, Springfield, IL 62794 9626, USA
    J Neurosci Methods 170:181-7. 2008
    ..These results show that NbActiv4 is an improvement to Neurobasal/B27 for cultured networks with an increased density of synapses and transmitter receptors which produces higher spontaneous spike rates in neuron networks...
  12. pmc Neuron network activity scales exponentially with synapse density
    G J Brewer
    Department of Neurology, Southern Illinois University School of Medicine, Springfield, IL 62794 9626, USA
    J Neural Eng 6:014001. 2009
    ....
  13. ncbi request reprint Thrombin receptor on rat primary hippocampal neurons: coupled calcium and cAMP responses
    Y Yang
    Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield 62794 1220, USA
    Brain Res 761:11-8. 1997
    ..These results may have implications for synaptic remodeling, learning and memory...
  14. ncbi request reprint Effects of acidosis on the distribution of processing of the beta-amyloid precursor protein in cultured hippocampal neurons
    G J Brewer
    Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield 62794 1220, USA
    Mol Chem Neuropathol 31:171-86. 1997
    ..If a similar process occurs in the presence of other cell types in the aging brain, acidosis may stimulate an extracellular deposition of amyloid and contribute to the pathogenesis of Alzheimer disease...
  15. pmc Epigenetic oxidative redox shift (EORS) theory of aging unifies the free radical and insulin signaling theories
    Gregory J Brewer
    Kenneth Stark Endowed Chair in Alzheimer Research, Southern Illinois University School of Medicine, Springfield, IL 62794 9626, USA
    Exp Gerontol 45:173-9. 2010
    ..Several steps in the pathway are amenable to reversal for exit from the vicious cycle of EORS. Examples from our work in the aging rodent brain as well as other aging models are provided...
  16. ncbi request reprint Age-related calcium changes, oxyradical damage, caspase activation and nuclear condensation in hippocampal neurons in response to glutamate and beta-amyloid
    Gregory J Brewer
    Medical Microbiology and Immunology, Southern Illinois University School of Medicine, P O Box 19626, Springfield, IL 62794 9626, USA
    Exp Gerontol 40:426-37. 2005
    ..Age-related glutamate toxicity involves other steps that lead to nuclear condensation, but neuron responses to calcium influx appear more important to cell death than the amount of influx...
  17. pmc Chronic electrical stimulation of cultured hippocampal networks increases spontaneous spike rates
    Gregory J Brewer
    Department of Neurology, Southern Illinois University School of Medicine, Springfield, IL 62794 9626, USA
    J Neurosci Methods 184:104-9. 2009
    ..The results suggest that chronic stimulation applied during network development recruits activity at 50% more electrodes and enables higher rates of spontaneous activity within bursts in cultured hippocampal networks...
  18. ncbi request reprint Precocious axons and improved survival of rat hippocampal neurons on lysine-alanine polymer substrates
    G J Brewer
    Southern Illinois University School of Medicine, Springfield 62794 1220, USA
    J Neurosci Methods 85:13-20. 1998
    ..These results indicate that LAS is a superior substrate to polylysine for growth of CNS neurons. LAS may be useful for regeneration of damaged circuits in the CNS as well as a substrate for connections to a neuroprosthesis...
  19. ncbi request reprint Age-related toxicity to lactate, glutamate, and beta-amyloid in cultured adult neurons
    G J Brewer
    Southern Illinois University School of Medicine, Springfield 62794 9626, USA
    Neurobiol Aging 19:561-8. 1998
    ..Similar results were found for A beta (1-40) (LD50 = 2 microM). These results suggest that aging imparts to individual cells an increased susceptibility to toxic substances relevant to neurodegenerative diseases...
  20. ncbi request reprint Age- and concentration-dependent neuroprotection and toxicity by TNF in cortical neurons from beta-amyloid
    J J Viel
    Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield, IL 62794-9626, USA
    J Neurosci Res 64:454-65. 2001
    ..These results are discussed in relation to neuroprotection and toxicity of the age-related pathology of AD...
  21. ncbi request reprint Age-related differences in NMDA responses in cultured rat hippocampal neurons
    C Cady
    Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, P.O. Box 19626, Springfield, IL 62794-9626, USA
    Brain Res 921:1-11. 2001
    ..996). Our data support the hypothesis that NMDAR responsiveness is altered during aging with an enhanced NMDA peak current in both old and embryonic neurons compared to middle-age neurons...
  22. ncbi request reprint Temperature and time interval for culture of postmortem neurons from adult rat cortex
    J J Viel
    Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield, Illinois 62794-9626, USA
    J Neurosci Res 64:311-21. 2001
    ..These findings should encourage donation of human postmortem brain neurons for studies on ischemia, adult pharmacology, and neurological disease...
  23. ncbi request reprint CTCF is essential for up-regulating expression from the amyloid precursor protein promoter during differentiation of primary hippocampal neurons
    Y Yang
    Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield 62794 9626, USA
    J Neurochem 73:2286-98. 1999
    ..These results suggest that CTCF is pivotal for the up-regulation of APP expression during synaptogenesis in primary neurons...
  24. pmc Chronic network stimulation enhances evoked action potentials
    A N Ide
    Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University, School of Medicine, Springfield, IL 62794 9626, USA
    J Neural Eng 7:16008. 2010
    ..These data suggest plastic network changes induced by chronic stimulation that enhance the reliability of information transmission and the efficiency of multisynaptic network communication...
  25. ncbi request reprint Culture of neurons from postmortem rat brain
    D Q McManus
    Department of Neurology, Southern Illinois University School Medicine, Springfield 62794 1220, USA
    Neurosci Lett 224:193-6. 1997
    ..Our results suggest that neurons may be cultured from autopsy donors who have died either with or without a neurodegenerative disease such as Alzheimer or Parkinson disease...
  26. ncbi request reprint Electrophysiology of embryonic, adult and aged rat hippocampal neurons in serum-free culture
    M S Evans
    Department of Neurology, Southern Illinois University School of Medicine, Springfield 62794, USA
    J Neurosci Methods 79:37-46. 1998
    ..Our results indicate that both embryonic and adult cultured neurons retain a clearly neuronal electrophysiological phenotype in Neurobasal/B27 serum-free medium...
  27. ncbi request reprint Upregulation of amyloid precursor protein gene promoter in rat primary hippocampal neurons by phorbol ester, IL-1 and retinoic acid, but not by reactive oxygen species
    Y Yang
    Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield, IL 62794 1220, USA
    Brain Res Mol Brain Res 60:40-9. 1998
    ..Since elevated levels of IL-1 have been observed in AD brain, IL-1 could contribute to development of Alzheimer's disease by stimulating APP synthesis in primary neurons...
  28. ncbi request reprint Pertussis toxin B-oligomer suppresses human immunodeficiency virus-1 Tat-induced neuronal apoptosis through feedback inhibition of phospholipase C-beta by protein kinase C
    S Jajoo
    Department of Pharmacology, Southern Illinois University School of Medicine, PO Box 19629, Springfield, IL 62794 9629, USA
    Neuroscience 151:525-32. 2008
    ..Furthermore, PTX-B suppresses HIV-1 Tat-mediated apoptosis by reducing its activation of PLC-beta through a PKC activation pathway...
  29. ncbi request reprint Long-term maintenance of patterns of hippocampal pyramidal cells on substrates of polyethylene glycol and microstamped polylysine
    D W Branch
    Biophysics Program, Beckman Institute, Urbana, IL, USA
    IEEE Trans Biomed Eng 47:290-300. 2000
    ..We conclude that these materials, especially PEG, are adequate for the maintenance of long-term patterned cultures of neurons. We believe that this is the first report of high-quality long-term patterning of cultured neurons...
  30. doi request reprint Age-related decreases in NAD(P)H and glutathione cause redox declines before ATP loss during glutamate treatment of hippocampal neurons
    Mordhwaj S Parihar
    Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, Illinois 62794 9626, USA
    J Neurosci Res 86:2339-52. 2008
    ..We conclude that age does not affect production of NADH by dehydrogenases but that old-aged neurons consume more NADH and glutathione, leading to a catastrophic decline in redox ratio...
  31. pmc Age-related changes to tumor necrosis factor receptors affect neuron survival in the presence of beta-amyloid
    Jigisha R Patel
    Department of Medical Microbiology, Immunology, and Cell Biology, Southern Illinois University, School of Medicine, Springfield, Illinois 62702, USA
    J Neurosci Res 86:2303-13. 2008
    ..These findings support the hypothesis that age-related changes in TNF-alpha surface receptor expression contribute to the neuronal loss associated with inflammation in Alzheimer's disease...
  32. ncbi request reprint Postmortem effect of pentobarbital anesthetic on survival of adult cortical neurons in primary culture
    John J Viel
    Department of Medical Microbiology and Immunology, School of Medicine, Southern Illinois University, Springfield, IL 62794 9626, USA
    Brain Res 1009:219-22. 2004
    ....
  33. ncbi request reprint Development of astroglial cells in patterned neuronal cultures
    Yoonkey Nam
    Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology, Daejeon, South Korea
    J Biomater Sci Polym Ed 18:1091-100. 2007
    ..Over the remaining period, astroglial cells proliferated and the co-localization was 80%. Hence, in these culture conditions astroglial cells develop 2-3 weeks later than neurons but remain highly co-localized with neurons...
  34. ncbi request reprint Age-related changes in neuronal glucose uptake in response to glutamate and beta-amyloid
    Jigisha R Patel
    Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield, Illinois 62794 9626, USA
    J Neurosci Res 72:527-36. 2003
    ..Neuron aging does not indicate a defect in normal glut3 function; rather, our results suggest that mechanisms regulating glucose uptake under stress fail to react in time to ensure cell survival...
  35. ncbi request reprint Active 3-D microscaffold system with fluid perfusion for culturing in vitro neuronal networks
    Laura Rowe
    School of Electrical and Computer Engineering, Georgia Institute of Technology, 791 Atlantic Drive, Atlanta, GA 30332, USA
    Lab Chip 7:475-82. 2007
    ..Furthermore, use of the active microscaffold system was demonstrated by culturing primary hippocampal embryonic rat pup neurons, and characterizing cell viability within the microscaffold system...
  36. ncbi request reprint Simultaneous age-related depolarization of mitochondrial membrane potential and increased mitochondrial reactive oxygen species production correlate with age-related glutamate excitotoxicity in rat hippocampal neurons
    Mordhwaj S Parihar
    Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, Illinois 62794, USA
    J Neurosci Res 85:1018-32. 2007
    ....
  37. ncbi request reprint Neuronal network structuring induces greater neuronal activity through enhanced astroglial development
    John C Chang
    Department of Radiology, Stanford University Medical Center, 300 Pasteur Drive, Stanford, CA 94305, USA
    J Neural Eng 3:217-26. 2006
    ..Together, this yielded greater overall neuronal activity as evaluated by the number of active electrodes. Our finding of astrocytic proliferation within serum-free culture is also novel...
  38. ncbi request reprint A modified microstamping technique enhances polylysine transfer and neuronal cell patterning
    John C Chang
    Department of Electrical and Computer Engineering, Beckman Institute, University of Illinois at Urbana Champaign, 405 N Mathews Ave, Urbana, IL 61801, USA
    Biomaterials 24:2863-70. 2003
    ....
  39. ncbi request reprint Gold-coated microelectrode array with thiol linked self-assembled monolayers for engineering neuronal cultures
    Yoonkey Nam
    Department of Electrical and Computer Engineering, Beckman Institute, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    IEEE Trans Biomed Eng 51:158-65. 2004
    ..The gold coating technique makes it possible to use the rich set of thiol-based surface modification techniques in combination with MEA recording...
  40. ncbi request reprint Apolipoprotein E4 inhibits, and apolipoprotein E3 promotes neurite outgrowth in cultured adult mouse cortical neurons through the low-density lipoprotein receptor-related protein
    Britto P Nathan
    Department of Biological Sciences, Eastern Illinois University, 600 Lincoln Avenue, Charleston, IL 61920, USA
    Brain Res 928:96-105. 2002
    ..These data suggest a potential mechanism whereby apoE4 may play a role in regenerative failure and accelerate the development of AD...

Research Grants12

  1. Age-Dependent Response of Hippocampal Neurons to Stress
    Gregory Brewer; Fiscal Year: 2006
    ..abstract_text> ..
  2. AGE DEPENDENT RESPONSE OF HIPPOCAMPAL NEURONS TO STRESS
    Gregory Brewer; Fiscal Year: 2000
    ..These studies hope to provide a better understanding of the age-related responses of cultured neurons to stressors thought to be important in the AD brain. ..
  3. Mitoenergetic Failure in Brain Aging
    Gregory J Brewer; Fiscal Year: 2010
    ..S. who will constitute 23% of the population in 2040, 70 million people. 13 million of these people are expected to have Alzheimer disease with expected health care costs of $250 billion. ..