Research Topics
Genomes and Genes | Sukesh R BhaumikSummaryAffiliation: Southern Illinois University School of Medicine Country: USA Publications
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Publications
Distinct regulatory mechanisms of eukaryotic transcriptional activation by SAGA and TFIIDSukesh R Bhaumik
Department of Biochemistry and Molecular Biology, Southern Illnois University School of Medicine, Carbondale, IL 62901, USA
Biochim Biophys Acta 1809:97-108. 2011..The regulatory mechanisms of eukaryotic transcriptional activation by SAGA and TFIID are discussed here. This article is part of a Special Issue entitled The 26S Proteasome: When degradation is just not enough!..
Analysis of in vivo targets of transcriptional activators by fluorescence resonance energy transferSukesh R Bhaumik
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901, USA
Methods 40:353-9. 2006..Here, we describe the FRET assay in general for analysis of the targets of transcriptional activators in living yeast cells. Such an assay can also be used as a general method to monitor protein-protein interactions in vivo...
Diverse regulatory mechanisms of eukaryotic transcriptional activation by the proteasome complexSukesh R Bhaumik
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901, USA
Crit Rev Biochem Mol Biol 43:419-33. 2008..Here, we discuss the intricate mechanisms of such proteasomal regulation of eukaryotic gene activation via multiple pathways...
Rad26p regulates the occupancy of histone H2A-H2B dimer at the active genes in vivoShivani Malik
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901, USA
Nucleic Acids Res 40:3348-63. 2012..Similar results are also found at other inducible non-GAL genes. Collectively, our results define a new role of Rad26p in orchestrating chromatin structure and hence transcription in vivo...
Regulation of chromatin assembly/disassembly by Rtt109p, a histone H3 Lys56-specific acetyltransferase, in vivoGeetha Durairaj
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, Illinois 62901, USA
J Biol Chem 285:30472-9. 2010....
Rad26p, a transcription-coupled repair factor, is recruited to the site of DNA lesion in an elongating RNA polymerase II-dependent manner in vivoShivani Malik
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, 1245 Lincoln Drive, Carbondale, IL 62901, USA
Nucleic Acids Res 38:1461-77. 2010....
The mRNA cap-binding complex stimulates the formation of pre-initiation complex at the promoter via its interaction with Mot1p in vivoShweta Lahudkar
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901, USA
Nucleic Acids Res 39:2188-209. 2011....
Rad26p, a transcription-coupled repair factor, promotes the eviction and prevents the reassociation of histone H2A-H2B dimer during transcriptional elongation in vivoShivani Malik
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901, USA
Biochemistry 51:5873-5. 2012..Thus, the absence of Rad26p leads to the generation of an atypical histone H2A-H2B dimer-enriched chromatin at the active coding sequence in vivo...
Sgf29p facilitates the recruitment of TATA box binding protein but does not alter SAGA's global structural integrity in vivoAbhijit Shukla
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, Illinois 62901, United States
Biochemistry 51:706-14. 2012..Taken together, these results support the idea that SAGA-associated Sgf29p facilitates the recruitment of TBP (and hence transcription) without altering the global structural integrity of SAGA in vivo...
The 19S proteasome subcomplex promotes the targeting of NuA4 HAT to the promoters of ribosomal protein genes to facilitate the recruitment of TFIID for transcriptional initiation in vivoBhawana Uprety
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901, USA
Nucleic Acids Res 40:1969-83. 2012....
The 19 s proteasome subcomplex establishes a specific protein interaction network at the promoter for stimulated transcriptional initiation in vivoShivani Malik
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, Illinois 62901, USA
J Biol Chem 284:35714-24. 2009..Together, these results provide significant insights as to how the 19 S proteasome subcomplex regulates the formation of the active transcription complex assembly (and, hence, transcriptional initiation) at the promoter in vivo...
Functional analysis of Rad14p, a DNA damage recognition factor in nucleotide excision repair, in regulation of transcription in vivoPriyasri Chaurasia
Department of Biochemistry and Molecular Biology, Southern Illinois University, School of Medicine, Carbondale, Illinois 62901, USA
J Biol Chem 288:793-806. 2013..Collectively, our results unveil a new role of Rad14p in stimulating transcription in addition to its well-known function in nucleotide excision repair...
Mixed lineage leukemia: histone H3 lysine 4 methyltransferases from yeast to humanShivani Malik
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901, USA
FEBS J 277:1805-21. 2010..These studies revealed a set of histone H3 lysine 4 methyltransferases with important cellular functions in different eukaryotes, as discussed here...
Stimulation of mRNA export by an F-box protein, Mdm30p, in vivoAbhijit Shukla
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, 1245 Lincoln Drive, Carbondale, IL 62901, USA
J Mol Biol 389:238-47. 2009..Together, our data demonstrate that Mdm30p selectively controls mRNA export independently of mitochondrial fusion, revealing a novel function of an F-box protein in mRNA export...
Nuclear export of mRNA and its regulation by ubiquitylationGeetha Durairaj
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL, USA
RNA Biol 6:531-5. 2009..Here, we highlight the recent advances of mRNA export and its regulation by ubiquitylation or the enzymes controlling this posttranslational modification...
The 26S proteasome complex: an attractive target for cancer therapySarah Frankland-Searby
Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901, USA
Biochim Biophys Acta 1825:64-76. 2012..Here, we discuss the 26S proteasome complex in carcinogenesis and different proteasome inhibitors with their potential therapeutic applications in treatment of numerous cancers...
Elongating RNA polymerase II is disassembled through specific degradation of its largest but not other subunits in response to DNA damage in vivoShivani Malik
Department of Biochemistry and Molecular Biology, Southern Illinois University, School of Medicine, Carbondale, Illinois 62901, USA
J Biol Chem 283:6897-905. 2008....
In vivo target of a transcriptional activator revealed by fluorescence resonance energy transferSukesh R Bhaumik
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 18:333-43. 2004..The FRET assay we describe is a general method that can be used to identify the in vivo targets of other activators...
