Philip R Hardwidge

Summary

Affiliation: South Dakota State University
Country: USA

Publications

  1. ncbi request reprint Proteomic analysis of the binding partners to enteropathogenic Escherichia coli virulence proteins expressed in Saccharomyces cerevisiae
    Philip R Hardwidge
    Veterinary Science Department, South Dakota State University, Brookings, SD, USA
    Proteomics 6:2174-9. 2006
  2. pmc Application of a split luciferase complementation assay for the detection of viral protein-protein interactions
    Qiji Deng
    Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, United States
    J Virol Methods 176:108-11. 2011
  3. pmc Enterotoxigenic Escherichia coli modulates host intestinal cell membrane asymmetry and metabolic activity
    Amber M Johnson
    Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, South Dakota 57007, USA
    Infect Immun 77:341-7. 2009
  4. pmc Nuclear localization of influenza B polymerase proteins and their binary complexes
    Qiji Deng
    Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, United States
    Virus Res 156:49-53. 2011
  5. doi request reprint Disruption of transepithelial resistance by enterotoxigenic Escherichia coli
    Amber M Johnson
    Department of Veterinary Science, South Dakota State University, Brookings, SD 57007, USA
    Vet Microbiol 141:115-9. 2010
  6. pmc Characterization of the NleF effector protein from attaching and effacing bacterial pathogens
    Frank Echtenkamp
    Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, SD 57007, USA
    FEMS Microbiol Lett 281:98-107. 2008
  7. pmc Heat-labile enterotoxin promotes Escherichia coli adherence to intestinal epithelial cells
    Amber M Johnson
    Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, South Dakota 57007, USA
    J Bacteriol 191:178-86. 2009
  8. doi request reprint Near-infrared fluorescence detection of ATP-biotin-mediated phosphoprotein labeling
    Xiaofei Gao
    Center for Infectious Disease Research and Vaccinology, South Dakota State University, Box 2175, 1119 North Campus Drive, Brookings, SD 57007, USA
    Biotechnol Lett 31:113-7. 2009
  9. ncbi request reprint Biochemical characterization of the enterotoxigenic Escherichia coli LeoA protein
    Eric A Brown
    Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, SD 57007, USA
    Microbiology 153:3776-84. 2007
  10. ncbi request reprint Susceptibility of human enterotoxigenic Escherichia coli isolates to growth inhibition by porcine intestinal epithelial cells
    Eric A Brown
    Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, SD 57007, USA
    FEMS Microbiol Lett 274:95-101. 2007

Research Grants

Collaborators

  • Feng Li
  • Dan Wang
  • Ruedi Aebersold
  • Mark E Wickham
  • James M Fleckenstein
  • B B Finlay
  • Nikhil A Thomas
  • D R Goodlett
  • Tony Pawson
  • Amber M Johnson
  • Radhey S Kaushik
  • Qiji Deng
  • Xiaofei Gao
  • Eric A Brown
  • Samantha Gruenheid
  • Wanyin Deng
  • Suvobrata Chakravarty
  • Thorsten Wolff
  • Xiaoxiao Xiang
  • Frank Echtenkamp
  • Ajitha Thanabalasuriar
  • Jinoh Kim
  • Jose L Puente
  • Elizabeth A Frey
  • Isabel Rodriguez-Escudero
  • Micah McCauley
  • Radhey Kaushik
  • Nicholas J Rotella
  • David H Francis
  • Amy Schutz-Geschwender
  • Alejandra Vazquez
  • Tessa Chaworth-Musters
  • Pavel Metalnikov
  • Randy Schekman
  • Paula I Lario
  • Elizabeth L Hartland
  • Keyrillos Rizg
  • Sau Fung Lee
  • Natalie C Strynadka
  • J Chris Fromme
  • Mark C Williams
  • Maria Molina
  • Richard A Pfuetzner
  • Victor J Cid
  • L James Maher
  • Yuling Li
  • Sansan Lee
  • Cesar Nombela
  • Natalie C J Strynakda

Detail Information

Publications20

  1. ncbi request reprint Proteomic analysis of the binding partners to enteropathogenic Escherichia coli virulence proteins expressed in Saccharomyces cerevisiae
    Philip R Hardwidge
    Veterinary Science Department, South Dakota State University, Brookings, SD, USA
    Proteomics 6:2174-9. 2006
    ..The dataset suggests several potential mammalian targets of these proteins that may guide future experimentation...
  2. pmc Application of a split luciferase complementation assay for the detection of viral protein-protein interactions
    Qiji Deng
    Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, United States
    J Virol Methods 176:108-11. 2011
    ..The described SLCA is highly specific and easy to perform, and thus may be useful for studying protein-protein interactions in viral diseases...
  3. pmc Enterotoxigenic Escherichia coli modulates host intestinal cell membrane asymmetry and metabolic activity
    Amber M Johnson
    Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, South Dakota 57007, USA
    Infect Immun 77:341-7. 2009
    ....
  4. pmc Nuclear localization of influenza B polymerase proteins and their binary complexes
    Qiji Deng
    Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, United States
    Virus Res 156:49-53. 2011
    ..Results of our studies represent the first step toward the understanding of nuclear transport and molecular assembly within the influenza B polymerase complex...
  5. doi request reprint Disruption of transepithelial resistance by enterotoxigenic Escherichia coli
    Amber M Johnson
    Department of Veterinary Science, South Dakota State University, Brookings, SD 57007, USA
    Vet Microbiol 141:115-9. 2010
    ..Robust heat-labile enterotoxin-independent reduction of host transepithelial resistance was observed in the absence of tight junction protein mislocalization...
  6. pmc Characterization of the NleF effector protein from attaching and effacing bacterial pathogens
    Frank Echtenkamp
    Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, SD 57007, USA
    FEMS Microbiol Lett 281:98-107. 2008
    ..Challenge of gnotobiotic piglets also revealed a role for NleF in colonization of the piglet colon and rectoanal junction...
  7. pmc Heat-labile enterotoxin promotes Escherichia coli adherence to intestinal epithelial cells
    Amber M Johnson
    Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, South Dakota 57007, USA
    J Bacteriol 191:178-86. 2009
    ..Increased adherence was not due to changes in the surface expression of the host receptor for the K88ac adhesin. Evidence is also presented for a role for bacterial sensing of host-derived cAMP in promoting adherence to host cells...
  8. doi request reprint Near-infrared fluorescence detection of ATP-biotin-mediated phosphoprotein labeling
    Xiaofei Gao
    Center for Infectious Disease Research and Vaccinology, South Dakota State University, Box 2175, 1119 North Campus Drive, Brookings, SD 57007, USA
    Biotechnol Lett 31:113-7. 2009
    ..NIR signal intensity was dependent on substrate and ATP-biotin concentrations...
  9. ncbi request reprint Biochemical characterization of the enterotoxigenic Escherichia coli LeoA protein
    Eric A Brown
    Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, SD 57007, USA
    Microbiology 153:3776-84. 2007
    ..Deletion of leoA rendered ETEC H10407 non-motile, through apparent periplasmic accumulation of FliC...
  10. ncbi request reprint Susceptibility of human enterotoxigenic Escherichia coli isolates to growth inhibition by porcine intestinal epithelial cells
    Eric A Brown
    Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, SD 57007, USA
    FEMS Microbiol Lett 274:95-101. 2007
    ..Susceptibility to porcine intestinal epithelial cells appears to be restricted to ETEC and not E. coli O157:H7 disease isolates...
  11. ncbi request reprint Enteropathogenic Escherichia coli type III effectors alter cytoskeletal function and signalling in Saccharomyces cerevisiae
    Isabel Rodriguez-Escudero
    Departamento de Microbiologia II, Facultad de Farmacia, Universidad Complutense de Madrid, Pza Ramón y Cajal s n, 28040 Madrid, Spain
    Microbiology 151:2933-45. 2005
    ..The utility of the yeast model for functional analyses of EPEC pathogenesis is discussed...
  12. ncbi request reprint DNA bending by bZIP charge variants: a unified study using electrophoretic phasing and fluorescence resonance energy transfer
    Philip R Hardwidge
    Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, Minnesota 55905, USA
    Biochemistry 41:7732-42. 2002
    ....
  13. ncbi request reprint Dominant effect of protein charge rather than protein shape in apparent DNA bending by engineered bZIP domains
    Philip R Hardwidge
    Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, Minnesota 55905, USA
    Biochemistry 41:8277-88. 2002
    ....
  14. ncbi request reprint Reflections on apparent DNA bending by charge variants of bZIP proteins
    Philip R Hardwidge
    Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, MN 55905, USA
    Biopolymers 69:110-7. 2003
    ..We report that two conventional DNA bending assays that rely on DNA ligation cannot be applied to analysis of the bZIP peptides we studied due to ligation inhibition...
  15. ncbi request reprint Proteomic analysis of the intestinal epithelial cell response to enteropathogenic Escherichia coli
    Philip R Hardwidge
    Biotechnology Laboratory, University of British Columbia, Wesbrook Building, 6174 University Boulevard, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 279:20127-36. 2004
    ..These data provide a framework for future biochemical analyses of host-pathogen interactions...
  16. pmc Regulation of type III secretion hierarchy of translocators and effectors in attaching and effacing bacterial pathogens
    Wanyin Deng
    Michael Smith Laboratories, University of British Columbia, 301 2185, East Mall, Vancouver, British Columbia V6T 1Z4, Canada
    Infect Immun 73:2135-46. 2005
    ....
  17. pmc Dual binding modes for an HMG domain from human HMGB2 on DNA
    Micah McCauley
    Department of Physics and Center for Interdisciplinary Research on Complex Systems, Northeastern University, Boston, Massachusetts 02115, USA
    Biophys J 89:353-64. 2005
    ..However, at higher protein concentrations, a cooperative filament mode is observed instead of the hinge binding. This mode may be uniquely characterized by this high-force optical tweezers experiment...
  18. ncbi request reprint The bacterial virulence factor NleA inhibits cellular protein secretion by disrupting mammalian COPII function
    Jinoh Kim
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell Host Microbe 2:160-71. 2007
    ..Collectively, these findings indicate that disruption of COPII function in host cells contributes to the virulence of EPEC and EHEC...
  19. pmc Modulation of host cytoskeleton function by the enteropathogenic Escherichia coli and Citrobacter rodentium effector protein EspG
    Philip R Hardwidge
    Michael Smith Laboratories, University of British Columbia, 301 2185 East Mall, Vancouver, BC V6T 1Z4, Canada
    Infect Immun 73:2586-94. 2005
    ....
  20. pmc Charge neutralization and DNA bending by the Escherichia coli catabolite activator protein
    Philip R Hardwidge
    Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, MN 55905, USA
    Nucleic Acids Res 30:1879-85. 2002
    ..We suggest that asymmetric phosphate neutralization contributes favorably to DNA bending by CAP, but cannot account for the full DNA deformation...

Research Grants2

  1. Mechanisms of pathogenic E.coli-host cell interactions
    PHILIP HARDWIDGE; Fiscal Year: 2005
    ..The characterization of toxic E. coli proteins and the analysis of the intestinal response to these proteins will guide future vaccine and antibiotic designs. ..
  2. Emerging virulence factors in diarrheagenic E. coli pathogenesis
    PHILIP HARDWIDGE; Fiscal Year: 2006
    ..coli disease. ..