Ying Fang

Summary

Affiliation: South Dakota State University
Country: USA

Publications

  1. ncbi request reprint Peptide domains involved in the localization of the porcine reproductive and respiratory syndrome virus nucleocapsid protein to the nucleolus
    Raymond R R Rowland
    Department of Diagnostic Medicine and Pathobiology, 1800 Denison Avenue, Kansas State University, Manhattan, KS 66506, USA
    Virology 316:135-45. 2003
  2. pmc Validation of a blocking enzyme-linked immunosorbent assay for detection of antibodies against porcine reproductive and respiratory syndrome virus
    Neal H Ferrin
    Department of Veterinary Science, P O Box 2175, North Campus Dr, South Dakota State University, Brookings, SD 57007, USA
    Clin Diagn Lab Immunol 11:503-14. 2004
  3. pmc Efficient -2 frameshifting by mammalian ribosomes to synthesize an additional arterivirus protein
    Ying Fang
    Departments of Veterinary and Biomedical Science and Biology Microbiology, South Dakota State University, Brookings, SD 57007, USA
    Proc Natl Acad Sci U S A 109:E2920-8. 2012
  4. doi request reprint Development of genetic markers in the non-structural protein 2 region of a US type 1 porcine reproductive and respiratory syndrome virus: implications for future recombinant marker vaccine development
    Ying Fang
    Center for Infectious Disease Research and Vaccinology, Veterinary Science Department, South Dakota State University, Brookings, SD 57007, USA
    J Gen Virol 89:3086-96. 2008
  5. doi request reprint The PRRSV replicase: exploring the multifunctionality of an intriguing set of nonstructural proteins
    Ying Fang
    Department of Veterinary and Biomedical Science Department of Bio microbiology, South Dakota State University, Brookings, SD 57007 1396, USA
    Virus Res 154:61-76. 2010
  6. pmc A full-length cDNA infectious clone of North American type 1 porcine reproductive and respiratory syndrome virus: expression of green fluorescent protein in the Nsp2 region
    Ying Fang
    Center for Infectious Disease Research and Vaccinology, Department of Veterinary Science, Box 2175, South Dakota State University, Brookings, SD 57007 1396, USA
    J Virol 80:11447-55. 2006
  7. doi request reprint Immunodominant epitopes in nsp2 of porcine reproductive and respiratory syndrome virus are dispensable for replication, but play an important role in modulation of the host immune response
    Zhenhai Chen
    Veterinary Science Department, South Dakota State University, Brookings, SD 57007, USA
    J Gen Virol 91:1047-57. 2010
  8. doi request reprint Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus
    Steven R Lawson
    Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA
    Virus Res 163:461-8. 2012
  9. pmc Development of a fluorescent microsphere immunoassay for detection of antibodies against porcine reproductive and respiratory syndrome virus using oral fluid samples as an alternative to serum-based assays
    Robert J Langenhorst
    Department of Veterinary and Biomedical Science, South Dakota State University, Brookings, South Dakota, USA
    Clin Vaccine Immunol 19:180-9. 2012
  10. doi request reprint Development of an 8-plex Luminex assay to detect swine cytokines for vaccine development: assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination
    Steven Lawson
    Veterinary Science Department, South Dakota State University, North Campus Drive, Brookings, SD 57007, USA
    Vaccine 28:5356-64. 2010

Collaborators

Detail Information

Publications35

  1. ncbi request reprint Peptide domains involved in the localization of the porcine reproductive and respiratory syndrome virus nucleocapsid protein to the nucleolus
    Raymond R R Rowland
    Department of Diagnostic Medicine and Pathobiology, 1800 Denison Avenue, Kansas State University, Manhattan, KS 66506, USA
    Virology 316:135-45. 2003
    ..These results identify a single NLS involved in the transport of N from the cytoplasm and into nucleus. An additional peptide sequence, overlapping NLS-2, is involved in the further targeting of N to the nucleolus...
  2. pmc Validation of a blocking enzyme-linked immunosorbent assay for detection of antibodies against porcine reproductive and respiratory syndrome virus
    Neal H Ferrin
    Department of Veterinary Science, P O Box 2175, North Campus Dr, South Dakota State University, Brookings, SD 57007, USA
    Clin Diagn Lab Immunol 11:503-14. 2004
    ..The anticipated use of the bELISA is as a follow-up test to the IDEXX ELISA for determining the PRRSV serostatus of individual animals with unexpected positive test results from swine herds from which negative results are expected...
  3. pmc Efficient -2 frameshifting by mammalian ribosomes to synthesize an additional arterivirus protein
    Ying Fang
    Departments of Veterinary and Biomedical Science and Biology Microbiology, South Dakota State University, Brookings, SD 57007, USA
    Proc Natl Acad Sci U S A 109:E2920-8. 2012
    ..Our findings demonstrate that -2 PRF is a functional gene-expression mechanism in eukaryotes and add another layer to the complexity of arterivirus genome expression...
  4. doi request reprint Development of genetic markers in the non-structural protein 2 region of a US type 1 porcine reproductive and respiratory syndrome virus: implications for future recombinant marker vaccine development
    Ying Fang
    Center for Infectious Disease Research and Vaccinology, Veterinary Science Department, South Dakota State University, Brookings, SD 57007, USA
    J Gen Virol 89:3086-96. 2008
    ..This rationally designed marker virus will provide a basis for further development of PRRSV marker vaccines to assist with the control of PRRS...
  5. doi request reprint The PRRSV replicase: exploring the multifunctionality of an intriguing set of nonstructural proteins
    Ying Fang
    Department of Veterinary and Biomedical Science Department of Bio microbiology, South Dakota State University, Brookings, SD 57007 1396, USA
    Virus Res 154:61-76. 2010
    ....
  6. pmc A full-length cDNA infectious clone of North American type 1 porcine reproductive and respiratory syndrome virus: expression of green fluorescent protein in the Nsp2 region
    Ying Fang
    Center for Infectious Disease Research and Vaccinology, Department of Veterinary Science, Box 2175, South Dakota State University, Brookings, SD 57007 1396, USA
    J Virol 80:11447-55. 2006
    ..The availability of this North American type 1 infectious clone provides an important research tool for further study of the basic viral biology and pathogenic mechanisms of this group of type 1 PRRSV in the United States...
  7. doi request reprint Immunodominant epitopes in nsp2 of porcine reproductive and respiratory syndrome virus are dispensable for replication, but play an important role in modulation of the host immune response
    Zhenhai Chen
    Veterinary Science Department, South Dakota State University, Brookings, SD 57007, USA
    J Gen Virol 91:1047-57. 2010
    ..These results suggest that certain regions in nsp2 are non-essential for PRRSV replication but may play an important role in modulation of host immunity in vivo...
  8. doi request reprint Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus
    Steven R Lawson
    Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA
    Virus Res 163:461-8. 2012
    ....
  9. pmc Development of a fluorescent microsphere immunoassay for detection of antibodies against porcine reproductive and respiratory syndrome virus using oral fluid samples as an alternative to serum-based assays
    Robert J Langenhorst
    Department of Veterinary and Biomedical Science, South Dakota State University, Brookings, South Dakota, USA
    Clin Vaccine Immunol 19:180-9. 2012
    ..The development of oral fluid-based diagnostic tests will change the way we survey diseases in swine herds and improve our ability to cheaply and efficiently track PRRSV infections in both populations and individual animals...
  10. doi request reprint Development of an 8-plex Luminex assay to detect swine cytokines for vaccine development: assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination
    Steven Lawson
    Veterinary Science Department, South Dakota State University, North Campus Drive, Brookings, SD 57007, USA
    Vaccine 28:5356-64. 2010
    ....
  11. ncbi request reprint Construction of a full-length cDNA infectious clone of a European-like Type 1 PRRSV isolated in the U.S
    Ying Fang
    South Dakota State University, Brookings, USA
    Adv Exp Med Biol 581:605-8. 2006
  12. doi request reprint Identification of porcine reproductive and respiratory syndrome virus ORF1a-encoded non-structural proteins in virus-infected cells
    Yanhua Li
    Department of Veterinary and Biomedical Science, South Dakota State University, Brookings, SD, USA
    J Gen Virol 93:829-39. 2012
    ..Thus, this PRRSV antibody panel will be an important tool for future studies on the replication and pathogenesis of this major swine pathogen...
  13. pmc Nonstructural protein 2 of porcine reproductive and respiratory syndrome virus inhibits the antiviral function of interferon-stimulated gene 15
    Zhi Sun
    Department of Veterinary and Biomedical Science Department of Biology Microbiology, South Dakota State University, Brookings, South Dakota, USA
    J Virol 86:3839-50. 2012
    ..Taken together, our data demonstrate that ISG15 and ISGylation play an important role in the response to PRRSV infection and that nsp2 is a key factor in counteracting the antiviral function of ISG15...
  14. pmc Antibody response to porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural proteins and implications for diagnostic detection and differentiation of PRRSV types I and II
    Elizabeth Brown
    Department of Veterinary Science, Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, SD 57007 1396, USA
    Clin Vaccine Immunol 16:628-35. 2009
    ..This ELISA offers an additional tool for routine or follow-up diagnostics, as well as having substantial value in epidemiological surveys and outbreak investigations...
  15. ncbi request reprint Heterogeneity in Nsp2 of European-like porcine reproductive and respiratory syndrome viruses isolated in the United States
    Ying Fang
    Department of Veterinary Science, South Dakota State University, Brookings, SD 57007, USA
    Virus Res 100:229-35. 2004
    ..Furthermore, areas in Nsp2 with deletions and amino acid hypervariability localize to regions that are predicted to be immunologically important...
  16. ncbi request reprint Comparison of two DNA extractions and nested PCR, real-time PCR, a new commercial PCR assay, and bacterial culture for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces
    Jane Christopher-Hennings
    Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, SD 57007, USA
    J Vet Diagn Invest 15:87-93. 2003
    ..paratuberculosis from bovine feces and are valid alternatives to culture...
  17. pmc Characterization of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States
    Susan L Ropp
    Department of Veterinary Science, South Dakota State University, Brookings, South Dakota, USA
    J Virol 78:3684-703. 2004
    ....
  18. ncbi request reprint Production and characterization of monoclonal antibodies against the nucleocapsid protein of SARS-CoV
    Ying Fang
    South Dakota State University, Brookings, USA
    Adv Exp Med Biol 581:153-6. 2006
  19. pmc Genetic fusions of heat-labile (LT) and heat-stable (ST) toxoids of porcine enterotoxigenic Escherichia coli elicit neutralizing anti-LT and anti-STa antibodies
    Weiping Zhang
    Center for Infectious Disease Research and Vaccinology Veterinary Science Department, Box 2157, South Dakota State University, Brookings, SD 57006, USA
    Infect Immun 78:316-25. 2010
    ..This fusion strategy could provide instructive information to develop effective toxoid vaccines against ETEC-associated diarrhea in animals and humans...
  20. ncbi request reprint The 2b protein as a minor structural component of PRRSV
    Wai Hong Wu
    Department of Veterinary Science, South Dakota State University, Box 2175, Brookings, SD 57007, USA
    Virus Res 114:177-81. 2005
    ..This study suggests that 2b in PRRSV is similar to the E protein in EAV and forms a minor structural component of the virion...
  21. pmc The cysteine protease domain of porcine reproductive and respiratory syndrome virus nonstructural protein 2 possesses deubiquitinating and interferon antagonism functions
    Zhi Sun
    Department of Veterinary and Biomedical Science, South Dakota State University, Brookings, South Dakota 57007 1396, USA
    J Virol 84:7832-46. 2010
    ..This study represents a fundamental step in elucidating the role of nsp2 in PRRS pathogenesis and provides an important insight in future modified live-virus vaccine development...
  22. pmc Characterization of a porcine intestinal epithelial cell line for influenza virus production
    Zhi Sun
    Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA
    J Gen Virol 93:2008-16. 2012
    ..25 log(10) TCID(50) ml(-1), respectively. The availability of this swine-specific cell line represents a more relevant substrate for studies and growth of swine-origin influenza viruses...
  23. doi request reprint Targeted mutations in a highly conserved motif of the nsp1β protein impair the interferon antagonizing activity of porcine reproductive and respiratory syndrome virus
    Yanhua Li
    Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA
    J Gen Virol 94:1972-83. 2013
    ..Modifying the key residues in the highly conserved GKYLQRRLQ motif could attenuate virus growth and improve the cellular innate immune responses. ..
  24. doi request reprint Construction and immunogenicity evaluation of an epitope-based antigen against swine influenza A virus using Escherichia coli heat-labile toxin B subunit as a carrier-adjuvant
    Zhi Sun
    Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, United States
    Vet Microbiol 164:229-38. 2013
    ..This study establishes a platform for future construction of epitope-based vaccines against influenza A virus infection...
  25. pmc Characterization of the binding specificity of K88ac and K88ad fimbriae of enterotoxigenic Escherichia coli by constructing K88ac/K88ad chimeric FaeG major subunits
    Weiping Zhang
    The Center for Infectious Disease Research and Vaccinology, Department of Veterinary Science, South Dakota State University, Brookings, South Dakota 57007, USA
    Infect Immun 77:699-706. 2009
    ..These data indicate that the peptide including amino acids 125 to 163 of the FaeG subunit is essential for K88 variant-specific binding...
  26. pmc Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples
    Ying Fang
    Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA
    J Clin Microbiol 40:287-91. 2002
    ..Results were quantitated by using a standard curve derived from a plasmid containing IS900. A minimum quantity of 1.7 x 10(-4) pg of DNA, correlating to 1 to 8 CFU, was detected...
  27. ncbi request reprint Encephalitis virus persistence in California birds: experimental infections in mourning doves (Zenaidura macroura)
    William K Reisen
    Center for Vectorborne Diseases, School of Veterinary Medicine, Davis, University of California, CA 95616, USA
    J Med Entomol 41:462-6. 2004
    ..Our results indicated that adult mourning doves were an incompetent host for SLE virus and probably do not serve as a suitable overwintering or dispersal host for either WEE and SLE viruses...
  28. pmc Intracellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein: absence of nucleolar accumulation during infection and after expression as a recombinant protein in vero cells
    Raymond R R Rowland
    Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, 66506, USA
    J Virol 79:11507-12. 2005
    ..These data indicate that nuclear localization is not a conserved property among all nidoviruses...
  29. ncbi request reprint Role of corvids in epidemiology of west Nile virus in southern California
    William K Reisen
    Center for Vectorborne Diseases, Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
    J Med Entomol 43:356-67. 2006
    ..In contrast the uniform dispersion by territorial western scrub-jays resulted in a high, but evenly distributed, incidence of human disease in Kern County...
  30. ncbi request reprint Role of California (Callipepla californica) and Gambel's (Callipepla gambelii) quail in the ecology of mosquito-borne encephalitis viruses in California, USA
    William K Reisen
    Center for Vectorborne Diseases, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California 95616, USA
    Vector Borne Zoonotic Dis 6:248-60. 2006
    ....
  31. ncbi request reprint Does feeding on infected mosquitoes (Diptera: Culicidae) enhance the role of song sparrows in the transmission of arboviruses in California?
    William K Reisen
    Center for Vectorborne Diseases and Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
    J Med Entomol 44:316-9. 2007
    ..Comparatively few field-collected birds had antibodies against either WEEV or WNV, indicating that this species was infrequently bitten by infectious mosquitoes in nature and probably was of limited importance in viral amplification...
  32. pmc West Nile virus infection in tree squirrels (Rodentia: Sciuridae) in California, 2004-2005
    Kerry A Padgett
    Vector Borne Disease Section, Division of Communicable Disease Control, California Department of Health Services, Richmond, CA 94804, USA
    Am J Trop Med Hyg 76:810-3. 2007
    ..Tree squirrels are useful in WNV surveillance and provide localized evidence of WNV transmission to mammals...
  33. pmc Two-way antigenic cross-reactivity between severe acute respiratory syndrome coronavirus (SARS-CoV) and group 1 animal CoVs is mediated through an antigenic site in the N-terminal region of the SARS-CoV nucleoprotein
    Anastasia N Vlasova
    Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691 4096, USA
    J Virol 81:13365-77. 2007
    ..Identification of the cross-reactive and non-cross-reactive N-protein regions allows development of SARS-CoV-specific antibody assays for screening animal and human sera...
  34. pmc A case-control study of the relationship between hepatitis B virus DNA level and risk of hepatocellular carcinoma in Qidong, China
    Tao Tao Liu
    Department of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
    World J Gastroenterol 14:3059-63. 2008
    ..To investigate the role of hepatitis B virus (HBV) replication in the development of hepatocellular carcinoma (HCC), a nested case-control study was performed to study the relationship between HBV DNA level and risk of HCC...
  35. ncbi request reprint The short hairpin RNA driven by polymerase II suppresses both wild-type and lamivudine-resistant hepatitis B virus strains
    Guang Li Ren
    Department of Pediatrics, General Hospital of Guangzhou Military Command of PLA, Guangzhou, China
    Antivir Ther 12:865-76. 2007
    ..1/HBV, were constructed to target open reading frames (ORFs) of the HBV C and S genes from wild-type and drug-resistant strains. Treatment efficiency was also assessed...