J R Brown

Summary

Affiliation: SmithKline Beecham Pharmaceuticals
Country: USA

Publications

  1. ncbi request reprint Evidence for the early divergence of tryptophanyl- and tyrosyl-tRNA synthetases
    J R Brown
    Department of Biochemistry, Canadian Institute for Advanced Research, Dalhousie University, Sir Charles Tupper Building, Halifax, Nova Scotia, Canada
    J Mol Evol 45:9-16. 1997
  2. pmc Archaea and the prokaryote-to-eukaryote transition
    J R Brown
    Canadian Institute for Advanced Research, Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada
    Microbiol Mol Biol Rev 61:456-502. 1997
  3. ncbi request reprint Gene descent, duplication, and horizontal transfer in the evolution of glutamyl- and glutaminyl-tRNA synthetases
    J R Brown
    Department of Bioinformatics, SmithKline Beecham Pharmaceuticals, 1250 South Collegeville Road, UP1345, Collegeville PA 19426 0989, USA
    J Mol Evol 49:485-95. 1999
  4. pmc Systematic identification of selective essential genes in Helicobacter pylori by genome prioritization and allelic replacement mutagenesis
    A F Chalker
    Department of Anti Infective Research, SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania 19426, USA
    J Bacteriol 183:1259-68. 2001
  5. ncbi request reprint Evolutionary relationships of bacterial and archaeal glutamine synthetase genes
    J R Brown
    Canadian Institute for Advanced Research, Department of Biochemistry, Halifax, Nova Scotia
    J Mol Evol 38:566-76. 1994
  6. ncbi request reprint Universal trees based on large combined protein sequence data sets
    J R Brown
    Anti Microbial Bioinformatics Group, GlaxoSmithKline, 1250 South Collegeville Road, UP1345 P O Box 5089, Collegeville, Pennsylvania 19426 0989, USA
    Nat Genet 28:281-5. 2001
  7. pmc Root of the universal tree of life based on ancient aminoacyl-tRNA synthetase gene duplications
    J R Brown
    Canadian Institute for Advanced Research, Department of Biochemistry, Dalhousie University, Halifax, NS
    Proc Natl Acad Sci U S A 92:2441-5. 1995
  8. pmc 3-hydroxy-3-methylglutaryl coenzyme A reductase of Sulfolobus solfataricus: DNA sequence, phylogeny, expression in Escherichia coli of the hmgA gene, and purification and kinetic characterization of the gene product
    D A Bochar
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
    J Bacteriol 179:3632-8. 1997
  9. ncbi request reprint Genomic and phylogenetic perspectives on the evolution of prokaryotes
    J R Brown
    Microbial Bioinformatics Department, Division of Bioinformatics, Glaxo SmithKline Pharmaceuticals, 1250 South Collegeville Road, P O Box 5089, UP1345, Collegeville, Pennsylvania 19426 0989, USA
    Syst Biol 50:497-512. 2001
  10. pmc Identification, evolution, and essentiality of the mevalonate pathway for isopentenyl diphosphate biosynthesis in gram-positive cocci
    E I Wilding
    Department of Microbiology, SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania, 19426, USA
    J Bacteriol 182:4319-27. 2000

Collaborators

Detail Information

Publications14

  1. ncbi request reprint Evidence for the early divergence of tryptophanyl- and tyrosyl-tRNA synthetases
    J R Brown
    Department of Biochemistry, Canadian Institute for Advanced Research, Dalhousie University, Sir Charles Tupper Building, Halifax, Nova Scotia, Canada
    J Mol Evol 45:9-16. 1997
    ..Furthermore, reciprocally rooted phylogenies of TrpRS and TyrRS sequences confirm the closer evolutionary relationship of Archaea to eukaryotes by placing the root of the universal tree in the Bacteria...
  2. pmc Archaea and the prokaryote-to-eukaryote transition
    J R Brown
    Canadian Institute for Advanced Research, Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada
    Microbiol Mol Biol Rev 61:456-502. 1997
    ..One horizontal gene exchange might have involved the gram-positive Bacteria and the Archaea, while the other might have occurred between proteobacteria and eukaryotes and might have been mediated by endosymbiosis...
  3. ncbi request reprint Gene descent, duplication, and horizontal transfer in the evolution of glutamyl- and glutaminyl-tRNA synthetases
    J R Brown
    Department of Bioinformatics, SmithKline Beecham Pharmaceuticals, 1250 South Collegeville Road, UP1345, Collegeville PA 19426 0989, USA
    J Mol Evol 49:485-95. 1999
    ..Although all bacterial GlnRS form a monophyletic group, the broad phyletic distribution of this tRNA synthetase suggests that multiple gene transfers from eukaryotes to bacteria occurred shortly after the Archaea-eukaryote divergence...
  4. pmc Systematic identification of selective essential genes in Helicobacter pylori by genome prioritization and allelic replacement mutagenesis
    A F Chalker
    Department of Anti Infective Research, SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania 19426, USA
    J Bacteriol 183:1259-68. 2001
    ..We propose that the products of a number of these H. pylori-specific essential genes may be suitable targets for novel anti-H. pylori therapies...
  5. ncbi request reprint Evolutionary relationships of bacterial and archaeal glutamine synthetase genes
    J R Brown
    Canadian Institute for Advanced Research, Department of Biochemistry, Halifax, Nova Scotia
    J Mol Evol 38:566-76. 1994
    ..The GSI gene of Sulfolobus solfataricus, a member of the Crenarchaeota (extreme thermophiles), is exceptional and could not be definitely placed in either subdivision...
  6. ncbi request reprint Universal trees based on large combined protein sequence data sets
    J R Brown
    Anti Microbial Bioinformatics Group, GlaxoSmithKline, 1250 South Collegeville Road, UP1345 P O Box 5089, Collegeville, Pennsylvania 19426 0989, USA
    Nat Genet 28:281-5. 2001
    ..Thus, combined protein universal trees are highly congruent with SSU rRNA trees in their strong support for the separate monophyly of domains as well as the early evolution of thermophilic Bacteria...
  7. pmc Root of the universal tree of life based on ancient aminoacyl-tRNA synthetase gene duplications
    J R Brown
    Canadian Institute for Advanced Research, Department of Biochemistry, Dalhousie University, Halifax, NS
    Proc Natl Acad Sci U S A 92:2441-5. 1995
    ..vaginalis clustered with other eukaryotic ValRS genes, which may have been transferred from the mitochondrial genome to the nuclear genome, suggesting that this amitochondrial trichomonad once harbored an endosymbiotic bacterium...
  8. pmc 3-hydroxy-3-methylglutaryl coenzyme A reductase of Sulfolobus solfataricus: DNA sequence, phylogeny, expression in Escherichia coli of the hmgA gene, and purification and kinetic characterization of the gene product
    D A Bochar
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
    J Bacteriol 179:3632-8. 1997
    ..Unlike other HMG-CoA reductases, the enzyme was stable at 90 degrees C and was optimally active at pH 5.5 and 85 degrees C...
  9. ncbi request reprint Genomic and phylogenetic perspectives on the evolution of prokaryotes
    J R Brown
    Microbial Bioinformatics Department, Division of Bioinformatics, Glaxo SmithKline Pharmaceuticals, 1250 South Collegeville Road, P O Box 5089, UP1345, Collegeville, Pennsylvania 19426 0989, USA
    Syst Biol 50:497-512. 2001
    ..Promising new approaches to genomic analyses, which could add to our understanding prokaryotic evolution and help in their classification, are discussed...
  10. pmc Identification, evolution, and essentiality of the mevalonate pathway for isopentenyl diphosphate biosynthesis in gram-positive cocci
    E I Wilding
    Department of Microbiology, SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania, 19426, USA
    J Bacteriol 182:4319-27. 2000
    ..The mevalonate pathway thus represents a potential antibacterial target in the low-G+C gram-positive cocci...
  11. ncbi request reprint Identification, cloning, and expression of a functional phenylalanyl-tRNA synthetase (pheRS) from Staphylococcus aureus
    J W Savopoulos
    GlaxoSmithKline Pharmaceuticals, New Frontiers Science Park North, Coldharbour Road, Harlow, Essex CM19 5AD, United Kingdom
    Protein Expr Purif 21:470-84. 2001
    ..aureus (Oxford Strain) and demonstrate that Michaelis-Menten parameters for both recombinant and native enzyme, at least for phenylalanine tRNA aminoacylation are comparable...
  12. ncbi request reprint DNA repair systems in archaea: mementos from the last universal common ancestor?
    J DiRuggiero
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD, USA
    J Mol Evol 49:474-84. 1999
    ....
  13. ncbi request reprint Biosynthesis of the linkage region of glycosaminoglycans: cloning and activity of galactosyltransferase II, the sixth member of the beta 1,3-galactosyltransferase family (beta 3GalT6)
    X Bai
    Department of Cellular and Molecular Medicine, Glycobiology Research and Training Center, University of California, San Diego, La Jolla, California 92093 0687, USA
    J Biol Chem 276:48189-95. 2001
    ..Its identity was confirmed in vivo by siRNA-mediated inhibition of glycosaminoglycan synthesis in HeLa S3 cells. Its localization in the medial Golgi indicates that this is the major site for assembly of the linkage region...
  14. pmc MtGimC, a novel archaeal chaperone related to the eukaryotic chaperonin cofactor GimC/prefoldin
    M R Leroux
    Max Planck Institut fur Biochemie, Department of Cellular Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    EMBO J 18:6730-43. 1999
    ..In light of the absence of Hsp70 chaperones in many archaea, GimC may fulfil an ATP-independent, Hsp70-like function in archaeal de novo protein folding...