Genomes and Genes
J R Brown
Affiliation: SmithKline Beecham Pharmaceuticals
- Evidence for the early divergence of tryptophanyl- and tyrosyl-tRNA synthetasesJ R Brown
Department of Biochemistry, Canadian Institute for Advanced Research, Dalhousie University, Sir Charles Tupper Building, Halifax, Nova Scotia, Canada
J Mol Evol 45:9-16. 1997..Furthermore, reciprocally rooted phylogenies of TrpRS and TyrRS sequences confirm the closer evolutionary relationship of Archaea to eukaryotes by placing the root of the universal tree in the Bacteria...
- Archaea and the prokaryote-to-eukaryote transitionJ R Brown
Canadian Institute for Advanced Research, Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada
Microbiol Mol Biol Rev 61:456-502. 1997..One horizontal gene exchange might have involved the gram-positive Bacteria and the Archaea, while the other might have occurred between proteobacteria and eukaryotes and might have been mediated by endosymbiosis...
- Gene descent, duplication, and horizontal transfer in the evolution of glutamyl- and glutaminyl-tRNA synthetasesJ R Brown
Department of Bioinformatics, SmithKline Beecham Pharmaceuticals, 1250 South Collegeville Road, UP1345, Collegeville PA 19426 0989, USA
J Mol Evol 49:485-95. 1999..Although all bacterial GlnRS form a monophyletic group, the broad phyletic distribution of this tRNA synthetase suggests that multiple gene transfers from eukaryotes to bacteria occurred shortly after the Archaea-eukaryote divergence...
- Systematic identification of selective essential genes in Helicobacter pylori by genome prioritization and allelic replacement mutagenesisA F Chalker
Department of Anti Infective Research, SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania 19426, USA
J Bacteriol 183:1259-68. 2001..We propose that the products of a number of these H. pylori-specific essential genes may be suitable targets for novel anti-H. pylori therapies...
- Evolutionary relationships of bacterial and archaeal glutamine synthetase genesJ R Brown
Canadian Institute for Advanced Research, Department of Biochemistry, Halifax, Nova Scotia
J Mol Evol 38:566-76. 1994..The GSI gene of Sulfolobus solfataricus, a member of the Crenarchaeota (extreme thermophiles), is exceptional and could not be definitely placed in either subdivision...
- Universal trees based on large combined protein sequence data setsJ R Brown
Anti Microbial Bioinformatics Group, GlaxoSmithKline, 1250 South Collegeville Road, UP1345 P O Box 5089, Collegeville, Pennsylvania 19426 0989, USA
Nat Genet 28:281-5. 2001..Thus, combined protein universal trees are highly congruent with SSU rRNA trees in their strong support for the separate monophyly of domains as well as the early evolution of thermophilic Bacteria...
- Root of the universal tree of life based on ancient aminoacyl-tRNA synthetase gene duplicationsJ R Brown
Canadian Institute for Advanced Research, Department of Biochemistry, Dalhousie University, Halifax, NS
Proc Natl Acad Sci U S A 92:2441-5. 1995..vaginalis clustered with other eukaryotic ValRS genes, which may have been transferred from the mitochondrial genome to the nuclear genome, suggesting that this amitochondrial trichomonad once harbored an endosymbiotic bacterium...
- 3-hydroxy-3-methylglutaryl coenzyme A reductase of Sulfolobus solfataricus: DNA sequence, phylogeny, expression in Escherichia coli of the hmgA gene, and purification and kinetic characterization of the gene productD A Bochar
Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
J Bacteriol 179:3632-8. 1997..Unlike other HMG-CoA reductases, the enzyme was stable at 90 degrees C and was optimally active at pH 5.5 and 85 degrees C...
- Genomic and phylogenetic perspectives on the evolution of prokaryotesJ R Brown
Microbial Bioinformatics Department, Division of Bioinformatics, Glaxo SmithKline Pharmaceuticals, 1250 South Collegeville Road, P O Box 5089, UP1345, Collegeville, Pennsylvania 19426 0989, USA
Syst Biol 50:497-512. 2001..Promising new approaches to genomic analyses, which could add to our understanding prokaryotic evolution and help in their classification, are discussed...
- Identification, evolution, and essentiality of the mevalonate pathway for isopentenyl diphosphate biosynthesis in gram-positive cocciE I Wilding
Department of Microbiology, SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania, 19426, USA
J Bacteriol 182:4319-27. 2000..The mevalonate pathway thus represents a potential antibacterial target in the low-G+C gram-positive cocci...
- Identification, cloning, and expression of a functional phenylalanyl-tRNA synthetase (pheRS) from Staphylococcus aureusJ W Savopoulos
GlaxoSmithKline Pharmaceuticals, New Frontiers Science Park North, Coldharbour Road, Harlow, Essex CM19 5AD, United Kingdom
Protein Expr Purif 21:470-84. 2001..aureus (Oxford Strain) and demonstrate that Michaelis-Menten parameters for both recombinant and native enzyme, at least for phenylalanine tRNA aminoacylation are comparable...
- DNA repair systems in archaea: mementos from the last universal common ancestor?J DiRuggiero
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD, USA
J Mol Evol 49:474-84. 1999....
- Biosynthesis of the linkage region of glycosaminoglycans: cloning and activity of galactosyltransferase II, the sixth member of the beta 1,3-galactosyltransferase family (beta 3GalT6)X Bai
Department of Cellular and Molecular Medicine, Glycobiology Research and Training Center, University of California, San Diego, La Jolla, California 92093 0687, USA
J Biol Chem 276:48189-95. 2001..Its identity was confirmed in vivo by siRNA-mediated inhibition of glycosaminoglycan synthesis in HeLa S3 cells. Its localization in the medial Golgi indicates that this is the major site for assembly of the linkage region...
- MtGimC, a novel archaeal chaperone related to the eukaryotic chaperonin cofactor GimC/prefoldinM R Leroux
Max Planck Institut fur Biochemie, Department of Cellular Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
EMBO J 18:6730-43. 1999..In light of the absence of Hsp70 chaperones in many archaea, GimC may fulfil an ATP-independent, Hsp70-like function in archaeal de novo protein folding...