PETER JOHN MYLER

Summary

Affiliation: Seattle Biomedical Research Institute
Country: USA

Publications

  1. ncbi request reprint Glyoxalase I from Leishmania donovani: a potential target for anti-parasite drug
    Prasad K Padmanabhan
    School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India
    Biochem Biophys Res Commun 337:1237-48. 2005
  2. pmc Gene organization and sequence analyses of transfer RNA genes in Trypanosomatid parasites
    Norma E Padilla-Mejía
    Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de Mexico, Av de los Barrios 1, Col Los Reyes Iztacala, Tlalnepantla, Edo de Mexico, Mexico
    BMC Genomics 10:232. 2009
  3. pmc Widespread variation in transcript abundance within and across developmental stages of Trypanosoma brucei
    Bryan C Jensen
    Seattle Biomedical Research Institute, 307 Westlake Ave North, Seattle, WA 98109, USA
    BMC Genomics 10:482. 2009
  4. pmc Patterns of gene recombination shape var gene repertoires in Plasmodium falciparum: comparisons of geographically diverse isolates
    Susan M Kraemer
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
    BMC Genomics 8:45. 2007
  5. pmc Histone acetylations mark origins of polycistronic transcription in Leishmania major
    Sean Thomas
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    BMC Genomics 10:152. 2009
  6. pmc Importing statistical measures into Artemis enhances gene identification in the Leishmania genome project
    Gautam Aggarwal
    Seattle Biomedical Research Institute 4 Nickerson Street, Seattle, WA 98109, USA
    BMC Bioinformatics 4:23. 2003
  7. pmc The Seattle Structural Genomics Center for Infectious Disease (SSGCID)
    P J Myler
    Seattle Biomedical Research Institute, 307 Westlake Ave N, Seattle, WA 98109 5219, USA
    Infect Disord Drug Targets 9:493-506. 2009
  8. doi request reprint Searching the Tritryp genomes for drug targets
    Peter J Myler
    Seattle Biomedical Research Institute, 307 Westlake Ave N, Suite 500, Seattle, Washington 98109 5219, USA
    Adv Exp Med Biol 625:133-40. 2008
  9. pmc Characterization of the RNA polymerase II and III complexes in Leishmania major
    Santiago Martinez-Calvillo
    Seattle Biomedical Research Institute, 307 Westlake Avenue N, Seattle, WA 98109 5219, USA
    Int J Parasitol 37:491-502. 2007
  10. pmc Leishmania major chromosome 3 contains two long convergent polycistronic gene clusters separated by a tRNA gene
    E A Worthey
    Seattle Biomedical Research Institute, 4 Nickerson Street, Seattle, WA 98109 1651, USA
    Nucleic Acids Res 31:4201-10. 2003

Research Grants

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Glyoxalase I from Leishmania donovani: a potential target for anti-parasite drug
    Prasad K Padmanabhan
    School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India
    Biochem Biophys Res Commun 337:1237-48. 2005
    ..Difference in the substrate specificity of the human and LDGLOI enzyme could be exploited for structure-based drug designing of selective inhibitors against the parasite...
  2. pmc Gene organization and sequence analyses of transfer RNA genes in Trypanosomatid parasites
    Norma E Padilla-Mejía
    Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de Mexico, Av de los Barrios 1, Col Los Reyes Iztacala, Tlalnepantla, Edo de Mexico, Mexico
    BMC Genomics 10:232. 2009
    ..To analyze the sequences and genomic organization of tRNA genes and other Pol III-transcribed genes, we have performed an in silico analysis of the Tritryps genome sequences...
  3. pmc Widespread variation in transcript abundance within and across developmental stages of Trypanosoma brucei
    Bryan C Jensen
    Seattle Biomedical Research Institute, 307 Westlake Ave North, Seattle, WA 98109, USA
    BMC Genomics 10:482. 2009
    ..Trypanosomatids represent a unique case in eukaryotes in that they transcribe protein-coding genes as large polycistronic units, and rarely regulate gene expression at the level of transcription initiation...
  4. pmc Patterns of gene recombination shape var gene repertoires in Plasmodium falciparum: comparisons of geographically diverse isolates
    Susan M Kraemer
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
    BMC Genomics 8:45. 2007
    ....
  5. pmc Histone acetylations mark origins of polycistronic transcription in Leishmania major
    Sean Thomas
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    BMC Genomics 10:152. 2009
    ..However, the mechanism by which Kinetoplastidae initiate transcription is unclear, and promoter sequences are undefined...
  6. pmc Importing statistical measures into Artemis enhances gene identification in the Leishmania genome project
    Gautam Aggarwal
    Seattle Biomedical Research Institute 4 Nickerson Street, Seattle, WA 98109, USA
    BMC Bioinformatics 4:23. 2003
    ..At SBRI, chromosomal sequence is annotated using a combination of trained and untrained non-consensus gene-prediction algorithms with ARTEMIS, an annotation platform with rich and user-friendly interfaces...
  7. pmc The Seattle Structural Genomics Center for Infectious Disease (SSGCID)
    P J Myler
    Seattle Biomedical Research Institute, 307 Westlake Ave N, Seattle, WA 98109 5219, USA
    Infect Disord Drug Targets 9:493-506. 2009
    ..Physical materials and data produced by SSGCID will be made available to the scientific community, with the aim that they will provide essential groundwork benefiting future research and drug discovery...
  8. doi request reprint Searching the Tritryp genomes for drug targets
    Peter J Myler
    Seattle Biomedical Research Institute, 307 Westlake Ave N, Suite 500, Seattle, Washington 98109 5219, USA
    Adv Exp Med Biol 625:133-40. 2008
    ..This vast amount of information now makes possible several new approaches for target identification and ushers in a post-genomic era for trypanosomatid drug discovery...
  9. pmc Characterization of the RNA polymerase II and III complexes in Leishmania major
    Santiago Martinez-Calvillo
    Seattle Biomedical Research Institute, 307 Westlake Avenue N, Seattle, WA 98109 5219, USA
    Int J Parasitol 37:491-502. 2007
    ....
  10. pmc Leishmania major chromosome 3 contains two long convergent polycistronic gene clusters separated by a tRNA gene
    E A Worthey
    Seattle Biomedical Research Institute, 4 Nickerson Street, Seattle, WA 98109 1651, USA
    Nucleic Acids Res 31:4201-10. 2003
    ..Numerous genes have been identified, including those for metabolic enzymes, kinases, transporters, ribosomal proteins, spliceosome components, helicases, an RNA-binding protein and a DNA primase subunit...
  11. ncbi request reprint Ploidy changes associated with disruption of two adjacent genes on Leishmania major chromosome 1
    Santiago Martinez-Calvillo
    Seattle Biomedical Research Institute, 307 Westlake Ave N, Seattle, WA 98109 5219, USA
    Int J Parasitol 35:419-29. 2005
    ..Further evidence of genome plasticity in Leishmania was provided by changes in chr1 copy number that occurred during in vitro growth of wild-type LmjF promastigotes and following replacement of a single copy of LmjF01.0760...
  12. pmc Crystal structures and proposed structural/functional classification of three protozoan proteins from the isochorismatase superfamily
    Jonathan Caruthers
    Biomolecular Structure Center M S 357742, University of Washington, Seattle, WA 98195, USA
    Protein Sci 14:2887-94. 2005
    ..The characterization of the active site is supported crystallographically by the presence of an unidentified ligand bound at the active site cysteine of the T. cruzi structure...
  13. ncbi request reprint Evaluation of differential gene expression in Leishmania major Friedlin procyclics and metacyclics using DNA microarray analysis
    Alka Saxena
    Department of Pathobiology, Seattle Biomedical Research Institute, 4 Nickerson Street, Seattle, WA 98109 1651, USA
    Mol Biochem Parasitol 129:103-14. 2003
    ..These studies confirmed the stage-specific expression of several known genes, as well as identifying a number of novel genes that are up-regulated in either procyclics or metacyclics...
  14. doi request reprint Structural genomics of pathogenic protozoa: an overview
    Erkang Fan
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Methods Mol Biol 426:497-513. 2008
    ..Fragment cocktail crystallography" for medical structural genomics is also described...
  15. pmc Trypanosoma brucei mitochondrial ribosomes: affinity purification and component identification by mass spectrometry
    Alena Zíková
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    Mol Cell Proteomics 7:1286-96. 2008
    ..Nevertheless most of the identified proteins have no homology outside Kinetoplastida implying very low conservation and/or a divergent function in kinetoplastid mitochondria...
  16. pmc A comprehensive analysis of Trypanosoma brucei mitochondrial proteome
    Aswini K Panigrahi
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Proteomics 9:434-50. 2009
    ..The identified proteins have predicted roles in macromolecular, metabolic, energy generating, and transport processes providing a comprehensive profile of the protein content and function of the T. brucei mt...
  17. ncbi request reprint Mitochondrial complexes in Trypanosoma brucei: a novel complex and a unique oxidoreductase complex
    Aswini K Panigrahi
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    Mol Cell Proteomics 7:534-45. 2008
    ..The relationship to divergent physiological processes in these pathogens is discussed...
  18. pmc Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major
    Tracy Arakaki
    Department of Biochemistry, University of Washington, Seattle, WA 98195 7742, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:175-9. 2006
    ..185 (Rfree = 0.229) at 1.60 A resolution. Structure and sequence comparisons based on Lmaj006129AAA suggest that proteins belonging to Pfam sequence families PF04543 and PF01878 may share a common ligand-binding motif...
  19. ncbi request reprint Heterologous expression of proteins from Plasmodium falciparum: results from 1000 genes
    Christopher Mehlin
    Structural Genomics of Pathogenic Protozoa, Box 357350, University of Washington, Seattle, WA 98195, USA
    Mol Biochem Parasitol 148:144-60. 2006
    ..A variety of factors conspire to make the heterologous expression of P. falciparum proteins challenging, and these observations lay the groundwork for a rational approach to prioritizing and, ultimately, eliminating these impediments...
  20. ncbi request reprint Using fragment cocktail crystallography to assist inhibitor design of Trypanosoma brucei nucleoside 2-deoxyribosyltransferase
    Jürgen Bosch
    Department of Biochemistry, Division of Infectious Disease, and Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195, USA
    J Med Chem 49:5939-46. 2006
    ....
  21. ncbi request reprint Transcription of Leishmania major Friedlin chromosome 1 initiates in both directions within a single region
    Santiago Martinez-Calvillo
    Seattle Biomedical Research Institute, and Department of Pathobiology, University of Washington, Seattle, WA 98109, USA
    Mol Cell 11:1291-9. 2003
    ..Thus, while in most eukaryotes each gene possesses its own promoter, a single region seems to drive the expression of the entire chr1 in LmjF...
  22. pmc Transcription initiation and termination on Leishmania major chromosome 3
    Santiago Martinez-Calvillo
    Seattle Biomedical Research Institute, Seattle, Washington 98109 1651, USA
    Eukaryot Cell 3:506-17. 2004
    ..Transcription on both strands terminates within the tRNA-gene region. Transient-transfection studies support the role of the tRNA-gene region as a transcription terminator for RNA polymerase II (Pol II) and Pol III, and also for Pol I...
  23. ncbi request reprint Genomic organization and expression of the expanded SCG/L/R gene family of Leishmania major: internal clusters and telomeric localization of SCGs mediating species-specific LPG modifications
    Deborah E Dobson
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Biochem Parasitol 146:231-41. 2006
    ..Potentially, telomeric localization of SCG genes may function primarily to facilitate gene conversion and the elaboration of functional evolutionary diversity in the degree of PG sc-galactosylation observed in other strains of L. major...
  24. ncbi request reprint Cloning, heterologous expression, and substrate specificities of protein farnesyltransferases from Trypanosoma cruzi and Leishmania major
    Frederick S Buckner
    Department of Medicine, University of Washington, Seattle 98195, USA
    Mol Biochem Parasitol 122:181-8. 2002
    ....
  25. ncbi request reprint Genomic organization and gene expression in a chromosomal region of Leishmania major
    Séverine Monnerat
    Department of Biochemistry, University of Lausanne, Ch des Boveresses 155, 1066 Epalinges, Switzerland
    Mol Biochem Parasitol 134:233-43. 2004
    ..In conclusion, the transcriptional organization of the Leishmania genome is complex, raising the possibility that computer predictions may not be comprehensive...
  26. ncbi request reprint A survey of Leishmania braziliensis genome by shotgun sequencing
    Eliane C Laurentino
    Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Av Bandeirantes, 3900, 14049 900, Brasil
    Mol Biochem Parasitol 137:81-6. 2004
    ..All data including sequences are also available at (www.ebi.ac.uk/embl/)...
  27. ncbi request reprint Comparative genomics of trypanosomatid parasitic protozoa
    Najib M El-Sayed
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 309:404-9. 2005
    ..Contrary to recent reports, our analyses reveal no evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont...
  28. ncbi request reprint The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease
    Najib M El-Sayed
    Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
    Science 309:409-15. 2005
    ....
  29. pmc The genome of the kinetoplastid parasite, Leishmania major
    Alasdair C Ivens
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    Science 309:436-42. 2005
    ..Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression...
  30. ncbi request reprint Sense and antisense transcripts in the histone H1 (HIS-1) locus of Leishmania major
    Sabina I Belli
    Institute of Biochemistry, University of Lausanne, Ch des Boveresses 155, Epalinges, 1066, Switzerland
    Int J Parasitol 33:965-75. 2003
    ..These findings suggest that the parasite has evolved in such a way as to maximise the transcription of its genome, a mechanism that might be important for it to maintain virulence...
  31. ncbi request reprint A novel active DNA topoisomerase I in Leishmania donovani
    Hector Villa
    Departamento de Farmacología y Toxicología INTOXCAL, Universidad de Leon, Campus de Vegazana sn, 24071 Leon, Spain
    J Biol Chem 278:3521-6. 2003
    ..DNA topoisomerase I activity was detected only when both genes (LdTOP1A and LdTOP1B) were co-expressed in a yeast expression system, suggesting the existence of a dimeric DNA topoisomerase I in Leishmania parasites...
  32. pmc New Trypanosoma cruzi repeated element that shows site specificity for insertion
    Renata T Souza
    Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, Sao Paulo, Brazil
    Eukaryot Cell 6:1228-38. 2007
    ..TcTREZO shows site specificity for insertion, suggesting that a sequence-specific endonuclease could be responsible for its insertion at a unique site...
  33. ncbi request reprint Expression of biopterin transporter (BT1) protein in Leishmania
    Vandana S Dole
    School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India
    FEMS Microbiol Lett 208:89-91. 2002
    ..These results indicate that 45-kDa BT1 protein in Leishmania is consistently and constitutively expressed in all the growth stages of the parasite...

Research Grants7

  1. Transcription of protein-coding genes in Leishmania
    Peter Myler; Fiscal Year: 2003
    ..abstract_text> ..
  2. Transcription of protein-coding genes in Leishmania
    Peter Myler; Fiscal Year: 2006
    ..abstract_text> ..
  3. Transcription of protein-coding genes in Leishmania
    Peter Myler; Fiscal Year: 2007
    ..abstract_text> ..
  4. Transcription of protein-coding genes in Leishmania
    PETER JOHN MYLER; Fiscal Year: 2010
    ..In this proposal, we will test this hypothesis and build on our current work to further characterize the chromatin-modifying enzymes that are involved in transcription initiation. ..